CN113265317A

CN113265317A - Microalgae culture equipment

Info

Publication number: CN113265317A
Application number: CN202110601212.5A
Authority: CN
Inventors: 周朝生; 陈星星; 朱洁; 胡园; 吴越; 陆荣茂; 柯爱英; 郑伊诺
Original assignee: Zhejiang Mariculture Research Institute
Current assignee: Zhejiang Mariculture Research Institute
Priority date: 2021-05-31
Filing date: 2021-05-31
Publication date: 2021-08-17

Abstract

The invention discloses microalgae culture equipment, which adopts the technical scheme that the microalgae culture equipment comprises a culture container, a feeding pipe, an inflation pipe, a discharge hole and a concentration monitor, wherein the concentration monitor comprises a detection module and a control module, the detection module is used for detecting whether carbon dioxide in the culture container reaches a specified threshold value, if so, a closing signal is sent, if not, an opening signal is sent, and the control module is coupled with a one-way valve and is used for receiving a signal of the detection module and controlling the opening and closing of the one-way valve. The invention adopts a closed culture mode, reduces carbon source loss and impurity algae pollution, improves the utilization efficiency of carbon dioxide, and effectively reduces the raw material cost of microalgae culture; the automatic filling of the carbon dioxide is realized by detecting the concentration of the carbon dioxide, so that the accurate management and control are more convenient, the unnecessary loss of the carbon dioxide is reduced, and the utilization rate of the carbon dioxide is improved; and the adoption of carbon dioxide as the carbon source for the growth of the algae also has good economic benefit and environmental benefit.

Description

Microalgae culture equipment

Technical Field

The invention relates to the technical field of algae cultivation, in particular to microalgae cultivation equipment.

Background

Algae is widely used as starter feed for shellfish and some seawater fishes and shrimps in seedling stage due to its advantages of small size, short propagation period, and high-density propagation.

At present, the domestic algae propagation method mainly takes an open cultivation barrel or a cement pond as a main part, and the propagation method generally causes pollution, so that a large amount of mixed algae grows and the quality of the propagated algae is influenced. The influence of weather and illumination intensity is large, and if continuous rainy days are encountered, the growth and propagation of algae are difficult; if the light intensity is not suitable, other miscellaneous algae will be propagated excessively. Moreover, harmful bacteria, pathogens and the like are easily introduced into the open propagation environment, so that the risk of breeding the shellfish and seawater fishes and shrimps is greatly increased.

Therefore, it is required to provide a closed culture system to reduce the influence of external environment on the algae liquid culture process and improve the yield of algae; the cost of carbon source needed by algae is as high as 60% of the cost of raw materials, and the cost of raw materials can be effectively reduced by using carbon dioxide as the carbon source for the auxiliary growth of the algae, so how to efficiently add carbon dioxide into the culture solution is an important aspect of the algae cultivation process.

The utilization rate of the carbon dioxide depends on the absorption rate of the algae liquid to the carbon dioxide, so that the management of the carbon dioxide concentration is increased on the basis of the closed culture system, the utilization efficiency of the carbon dioxide by the algae can be further improved, and the yield of high-quality algae can be further improved.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide microalgae culture equipment which can overcome the defect that open culture is greatly interfered by environment, and simultaneously monitors carbon dioxide to realize the effect of automatically filling carbon dioxide in the culture process.

In order to achieve the purpose, the invention provides the following technical scheme: the microalgae culturing equipment comprises a culturing container for culturing algae, a feeding pipe communicated with the culturing container and used for conveying algae liquid, an inflation pipe communicated with the culturing container and used for conveying carbon dioxide, a discharge hole arranged on the culturing container and used for collecting the algae, and a concentration monitor arranged on the culturing container and used for monitoring the concentration of the carbon dioxide in the culturing container, the other end of the gas-filled tube is connected with a carbon dioxide tank, a first valve is arranged at the joint of the gas-filled tube and the carbon dioxide tank, the first valve only allows carbon dioxide to flow into the culture container, the concentration monitor comprises a detection module and a control module, the detection module is used for detecting whether the carbon dioxide in the culture container reaches a specified threshold value, if so, and if not, sending an opening signal, wherein the control module is coupled with the first valve and used for receiving the signal of the detection module and controlling the opening and closing of the first valve.

Further, the culture container is arranged in a tubular structure, and the inner wall of the culture container is arranged in a smooth structure.

Further, the discharge port is arranged at the bottom of the culture container, the discharge port is communicated with a three-way pipe, the three-way pipe comprises a main pipe communicated with the discharge port, a first distribution pipe communicated with the main pipe and used for recovering algae liquid, and a second distribution pipe communicated with the main pipe and used for collecting algae, the three pipes are arranged in an inverted Y-shaped structure, a second valve is arranged on the main pipe, a sieve plate is arranged at an inlet of the first distribution pipe, the sieve plate is obliquely arranged from the main pipe to the second distribution pipe, the main pipe is vertically arranged, and the orthographic area of the main pipe on the sieve plate is smaller than the actual area of the sieve plate.

Further, one side of the screening plate facing the second distribution pipe is arranged in a smooth arc-shaped structure.

Further, pan feeding pipe, gas tube are the hose material, be connected with the swing subassembly that is used for driving the culture container and makes the rocking motion of falling on the culture container, the swing subassembly is including the support with culture container fixed connection, be connected with the support transmission and be used for driving the runner that culture container length direction went up one end and be the winding motion, be used for driving the rocker that culture container length direction went up and down the motion with the leg joint, be used for driving motor that first runner rotated and be used for supplying the base of runner and rocker installation, the runner output is connected with the bull stick, the bull stick becomes "Z" style of calligraphy structure setting, and its other end is connected on the support, the one end and the support fixed connection of rocker, the other end is articulated with the base.

Furthermore, the rotating rods are arranged in two and are respectively positioned on two opposite sides of one end of the support, and the rocking rods are arranged in two and are respectively positioned on two opposite sides of the other end of the support.

Further, a plurality of culture containers of fixedly connected with on the support, a plurality of culture container individual layers tiling sets up on the support, and the length direction of each culture container all corresponds the setting with the length direction of support.

Furthermore, the two opposite ends of the support in the length direction are provided with clamping seats for embedding the culture container, and the embedding grooves formed by surrounding of the clamping seats are matched with the size of the culture container.

Furthermore, an illuminator is detachably connected to the support and is positioned below the culture container, and the culture container is made of a light-transmitting material.

Furthermore, a temperature adjusting component is arranged in the culture container, an external control console is coupled with the temperature adjusting component, and the external control console sends out a control command to control the temperature adjusting component to release the specified temperature to the culture container.

In conclusion, the invention has the following beneficial effects: by adopting a closed culture mode, the carbon source loss and the impurity algae pollution are reduced, the utilization efficiency of carbon dioxide is improved, and the raw material cost for microalgae culture is effectively reduced; the automatic filling of the carbon dioxide is realized by detecting the concentration of the carbon dioxide, so that the accurate management and control are more convenient, the unnecessary loss of the carbon dioxide is reduced, and the utilization rate of the carbon dioxide is improved; and the adoption of carbon dioxide as the carbon source for the growth of the algae also has good economic benefit and environmental benefit.

Drawings

FIG. 1 is a schematic diagram of a microalgae cultivation apparatus;

FIG. 2 is a sectional view of a culture vessel;

fig. 3 is a schematic perspective view of the swing assembly.

Reference numerals: 1. a culture vessel; 2. a feeding pipe; 3. an inflation tube; 31. a first valve; 4. a discharge port; 41. a main pipe; 411. a valve; 42. a first distribution pipe; 421. sieving a sieve plate; 43. a second distribution pipe; 5. a swing assembly; 51. a support; 52. a rotating wheel; 521. a rotating rod; 53. a rocker; 54. a motor; 55. a base; 6. an illuminator.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and examples. In which like parts are designated by like reference numerals. It should be noted that the terms "front," "back," "left," "right," "upper" and "lower" used in the following description refer to directions in the drawings, and the terms "bottom" and "top," "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.

Referring to fig. 1-2, the microalgae cultivation apparatus comprises a cultivation container 1 for cultivating algae, a feeding pipe 2 communicated with the cultivation container 1 for conveying algae liquid, an inflation pipe 3 communicated with the cultivation container 1 for conveying carbon dioxide, a discharge port 4 arranged on the cultivation container 1 for collecting algae, and a concentration monitor arranged on the cultivation container 1 for monitoring the concentration of carbon dioxide in the cultivation container 1.

The algae liquid is a mixture of algae seeds and a culture medium liquid, the algae liquid enters the culture container 1 through the feeding pipe 2, and the gas filling pipe 3 conveys a carbon source required by the growth of algae bodies, so that the algae bodies perform photosynthesis to absorb carbon dioxide.

The culture container 1 can be placed outdoors and takes sunlight as a light source; the LED lamp can also be arranged indoors and takes lamplight as a light source.

The other end of the gas-filled tube 3 is connected with a carbon dioxide tank, a first valve 31 is arranged at the joint of the gas-filled tube 3 and the carbon dioxide tank, the first valve 31 preferably adopts a one-way valve only for carbon dioxide to flow into the culture container 1, the concentration monitor comprises a detection module and a control module, the detection module is used for detecting whether carbon dioxide in the culture container 1 reaches a specified threshold value, if so, a closing signal is sent, if not, an opening signal is sent, and the control module is coupled with the first valve 31 and used for receiving the signal of the detection module and controlling the first valve 31 to be opened and closed.

The above arrangement realizes timely supplement of carbon dioxide according to the change of the concentration of carbon dioxide in the culture container 1 in the culture process, thereby realizing automatic filling of carbon dioxide under the specified concentration of carbon dioxide. In the process of cultivating microalgae, the demand of carbon dioxide is not constant, and the culture container 1 belongs to a closed space, once the carbon dioxide which is previously conveyed is absorbed by the algae without being supplemented, the algae can grow slowly, the harvest of the algae can not be realized efficiently, and the efficiency is influenced. The carbon dioxide concentration monitoring and automatic filling realize the regulation and control of carbon dioxide delivery according to the growth period of the algae, and realize continuous supply, so that the algae can not absorb carbon dioxide intermittently, thus realizing high-efficiency cultivation and improving the production efficiency.

The culture vessel 1 is arranged in a tubular structure, and the inner wall of the culture vessel 1 is arranged in a smooth structure. The tubular structure can effectively reduce the deposition of culture nutrients at the bottom of the culture container 1, and the inner wall of the tubular structure adopts a smooth structure, so that the phenomenon of wall hanging of the algae in the culture container 1 in the cultivation process is reduced.

The discharge port 4 is arranged at the bottom of the culture container 1, the discharge port 4 is communicated with a three-way pipe, the three-way pipe comprises a main pipe 41 communicated with the discharge port 4, a first distribution pipe 42 communicated with the feeding pipe 2 and used for recovering algae liquid, and a second distribution pipe 43 communicated with the outside and used for collecting algae bodies, the three pipes are arranged in an inverted Y-shaped structure, a second valve 411 is arranged on the main pipe 41, a sieving plate 421 is arranged at the inlet of the first distribution pipe 42, and the sieving plate 421 is obliquely arranged from the main pipe 41 to the second distribution pipe 43.

The main pipe 41 is vertically arranged, and the projection area of the main pipe 41 on the sieving plate 421 is smaller than the actual area of the sieving plate 421. This arrangement ensures that the algae liquid flowing down from the main pipe 41 can first pass through the sieve plate 421 for separation and then flow into the second distribution pipes 43.

Mature algae meeting the harvest volume can be trapped by the sieve plate 421 and cannot enter the first distribution pipe 42, and then the formed algae meeting the conditions can enter the second distribution pipe 43 and be output by matching with the inclination angle of the sieve plate 421 along with the subsequent washing of the algae liquid;

the algae liquid entering the first distribution pipe 42 contains semi-mature algae which do not meet harvest conditions and culture medium liquid which nutrient components are not completely absorbed, the semi-mature algae can be extracted by further sieving after the algae liquid is conveyed out by the first distribution pipe 42, and the communication between the first distribution pipe 42 and the feeding pipe 2 means that the semi-mature algae can be conveyed to the culture container 1 through the feeding pipe 2 after being recovered. Therefore, the algae can be cultivated circularly and the harvest quality is improved.

The side of the screening deck 421 facing the second distribution pipes 43 is arranged in a smooth arc-shaped configuration. The arc structure here is the convex arc of directional second distributing pipe 43, because when passing through sieve plate 421, culture medium liquid and small-size algae body get into first distributing pipe 42, and lacked the effect of carrying of culture medium liquid for large-size algae body is carried inconveniently, and above-mentioned setting can reduce the hindrance of shaping algae body on sieve plate 421, can make the shaping algae body pass through sieve plate 421 back and smoothly get into second distributing pipe 43 and export.

The feeding pipe 2 and the gas filling pipe 3 are both connected to the side of the culture container 1, and the feeding pipe 2 is preferably arranged above the side of the culture container 1, so that the algae liquid can be quickly transmitted by using gravity, and meanwhile, the algae liquid is prevented from flowing backwards; the discharge port 4 is preferably arranged at the bottom of the culture container 1 and can also play a role in quickly discharging mature algae; the gas-filled tube 3 is arranged at the side of the culture container 1, so that carbon dioxide can be uniformly transmitted to the liquid space and the white space in the culture container 1, and the algae in the culture container 1 can be fully contacted with the carbon dioxide, thereby fully absorbing and utilizing the carbon source provided by the carbon dioxide.

The feeding pipe 2 and the gas filling pipe 3 are made of flexible pipe materials, the culture container 1 is connected with a swinging component 5 for driving the culture container 1 to do swinging motion, the swinging component 5 comprises a support 51 fixedly connected with the culture container 1, a rotating wheel 52 in transmission connection with the support 51 and used for driving one end of the culture container 1 to do winding motion in the length direction, a rocker 53 connected with the support 51 and used for driving the other end of the culture container 1 to do swinging motion in the length direction, a motor 54 used for driving the first rotating wheel 52 to rotate, and a base 55 used for mounting the rotating wheel 52 and the rocker 53, the output end of the rotating wheel 52 is connected with a rotating rod 521, the rotating rod 521 is arranged in a Z-shaped structure, the other end of the rotating rod is connected to the support 51, one end of the rocker 53 is fixedly connected with the support 51, and the other end of the rocker 53 is hinged to the base 55.

Swing subassembly 5 drives culture container 1 and makes the swinging movement that lodges, and its purpose is avoided the in-process that the alga body was cultivated in culture container 1 to produce the phenomenon of adherence, and makes culture container 1 not immovable in the complete cultivation in-process of the alga body through swing subassembly 5, but can appear rocking for shake appears in the algae liquid among the culture container 1, and the alga body that carries in the algae liquid just can be in planktonic state all the time, and can not adhere to on culture container 1's inner wall. This has the following advantages: when the algae needs to be harvested, the algae in the culture container 1 can be completely and smoothly taken out;

swing subassembly 5 drives culture container 1 and is made fluctuation motion back and forth along its length direction, and is softer for other macroseism sense motions, can not harm culture container 1 on the one hand, and on the other hand has also avoided leading to the excessive volatilization of carbon dioxide in the liquid space because of violent motion. Preferably, an air outlet can be further provided on the culture container 1 for recovering air, and it is known that the algae releases oxygen after absorbing carbon dioxide for photosynthesis, and the bracket 51 drives the culture container 1 to move, which also causes partial carbon dioxide to overflow, so that the exhausted air can be periodically recovered through the air outlet, and the recycling of the overflowed carbon dioxide is realized.

The two rotating rods 521 are respectively positioned at two opposite sides of one end of the bracket 51, and the two rocking rods 53 are respectively positioned at two opposite sides of the other end of the bracket 51. The arrangement makes the base 55 support the bracket 51 more strongly and more stably.

The plurality of culture containers 1 are fixedly connected to the support 51, the plurality of culture containers 1 are flatly laid on the support 51 in a single layer, and the length direction of each culture container 1 corresponds to the length direction of the support 51. Swing subassembly 5's effect mainly is that the algae liquid that drives in the culture vessel 1 vibrates to avoid the algae body in the culture vessel 1 to appear adherence phenomenon at the cultivation in-process, reduced the degree of difficulty of receiving into the algae body, consequently, set up a plurality of culture vessel 1 on support 51, not only do not change culture vessel 1 along with the state of the fluctuation swing motion that support 51 produced, use a set of swing subassembly 5 to realize a plurality of culture vessel 1 simultaneous movement moreover, save space more, the energy saving.

The installation mode of a plurality of culture containers 1 on support 51 preferably adopts single file/list structure, and each culture container 1 orientation unanimous, is favorable to gas tube 3, pan feeding pipe 2 more regular, orderly access like this, avoids intertwining, also can make the light coverage area not obstructed simultaneously.

The opposite ends of the bracket 51 in the length direction are provided with clamping seats for embedding the culture container 1, and the embedding grooves formed by the surrounding of the clamping seats are matched with the size of the culture container 1.

A silica gel pad is arranged on one side surface of the clamping seat abutting against the culture container 1. The arrangement of the silica gel pad can enhance the contact friction force between the clamping seat and the culture container 1, so that the installation stability of the culture container 1 is enhanced; on the other hand, the silica gel pad has certain elasticity, and can play a role in shock absorption in the process that the bracket 51 drives the culture container 1 to move.

The illuminator 6 is detachably connected to the support 51, the illuminator 6 is positioned below the culture container 1, and the culture container 1 is made of a light-transmitting material. The illuminator 6 preferably uses a cold white fluorescent tube as a light source, and does not change the temperature of the culture due to the illumination, which facilitates precise control of the culture temperature.

The single-layer arrangement can avoid overlapping and blocking light rays, and the light-transmitting property of the culture container 1 is matched with the illuminator 6 arranged below the culture container, so that the light rays of the illuminator 6 radially cover the whole culture container 1, and sufficient and stable light energy is provided for the algae in the culture container 1. On the other hand, the illuminator 6 is arranged below the culture container 1, and the culture container 1 can be used as a protective device to avoid damage to the illuminator 6 caused by external environment interference, for example, in an outdoor rainy day environment, rain water infiltration can cause the illuminator 6 to generate a disconnection risk. Further, a rain shield may be provided on the side surface of the culture container 1, or a light-transmitting protective cover may be provided outside the illuminator 6.

The illuminator 6 can also be connected with an external controller, and the illumination intensity, illumination time and the like can be regulated and controlled through the external controller, so that the intelligent control is more realized.

The culture container 1 is internally provided with a temperature adjusting component which is coupled with an external console, and the external console sends a control instruction to control the temperature adjusting component to release the specified temperature to the culture container 1. The temperature control in the microalgae culture process is realized by the arrangement, so that the culture conditions of the microalgae are optimized and controllable.

The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above-mentioned embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.

Claims

1. Microalgae culture equipment is characterized in that: comprises a culture container (1) for culturing algae, a feeding pipe (2) communicated with the culture container (1) and used for conveying algae liquid, an inflation pipe (3) communicated with the culture container (1) and used for conveying carbon dioxide, a discharge hole (4) arranged on the culture container (1) and used for collecting algae, and a concentration monitor arranged on the culture container (1) and used for monitoring the concentration of the carbon dioxide in the culture container (1), wherein the other end of the inflation pipe (3) is connected with a carbon dioxide tank, a first valve (31) is arranged at the joint of the inflation pipe (3) and the carbon dioxide tank, the concentration monitor comprises a detection module and a control module, the detection module is used for detecting whether the carbon dioxide in the culture container (1) reaches a specified threshold value, if yes, a closing signal is sent, if not, an opening signal is sent, and the control module is coupled with the first valve (31), the valve is used for receiving the signal of the detection module and controlling the opening and closing of the first valve (31).

2. The microalgae cultivation apparatus as claimed in claim 1, wherein: the utility model discloses a cultivation container, including culture container (1), discharge gate (4) intercommunication have the three-way pipe, the three-way pipe is including being responsible for (41) with discharge gate (4) intercommunication, being used for retrieving first distributing pipe (42) of algae liquid with pan feeding pipe (2) intercommunication, being used for receiving into second distributing pipe (43) of algae body with external intercommunication, and the three becomes "Y" structure setting, be provided with second valve (411) on being responsible for (41), the entry of first distributing pipe (42) is provided with sieve board (421), cross sieve board (421) and set up to second distributing pipe (43) direction slope by being responsible for (41), be responsible for (41) vertical setting, the orthographic projection area of being responsible for (41) on sieve board (421) is less than the actual area of passing sieve board (421).

3. The microalgae cultivation apparatus as claimed in claim 2, wherein: one side of the screening plate (421) facing the second distribution pipe (43) is arranged in a smooth arc structure.

4. The microalgae cultivation apparatus as claimed in claim 1, wherein: the feeding pipe (2) and the inflation pipe (3) are made of hose materials, the culture container (1) is connected with a swing assembly (5) which is used for driving the culture container (1) to do swinging motion, the swing assembly (5) comprises a support (51) fixedly connected with the culture container (1), a rotating wheel (52) which is in transmission connection with the support (51) and is used for driving one end of the culture container (1) to do winding motion in the length direction, a rocker (53) which is connected with the support (51) and is used for driving the other end of the culture container (1) to do swinging motion in the length direction, a motor (54) which is used for driving the first rotating wheel (52) to rotate, and a base (55) which is used for installing the rotating wheel (52) and the rocker (53), the output end of the rotating wheel (52) is connected with a rotating rod (521), the rotating rod (521) is arranged in a Z-shaped structure, and the other end of the rotating rod is connected with the support (51), one end of the rocker (53) is fixedly connected with the bracket (51), and the other end of the rocker is hinged with the base (55).

5. The microalgae cultivation apparatus as claimed in claim 4, wherein: the number of the rotating rods (521) is two, the rotating rods are respectively located on two opposite sides of one end of the support (51), and the number of the rocking rods (53) is two, and the rocking rods are respectively located on two opposite sides of the other end of the support (51).

6. The microalgae cultivation apparatus as claimed in claim 5, wherein: the culture container is characterized in that a plurality of culture containers (1) are fixedly connected to the support (51), the culture containers (1) are flatly laid on the support (51) in a single layer mode, and the length direction of each culture container (1) corresponds to the length direction of the support (51).

7. The microalgae cultivation apparatus as claimed in claim 6, wherein: the opposite two ends of the support (51) in the length direction are provided with clamping seats for embedding the culture container (1), and the embedding grooves formed by surrounding of the clamping seats are matched with the size of the culture container (1).

8. The microalgae cultivation apparatus as claimed in claim 7, wherein: and a silica gel pad is arranged on one side surface of the clamping seat abutting against the culture container (1).

9. An apparatus for cultivating microalgae according to claim 4 or 8, wherein: the illuminating device (6) is detachably connected to the support (51), the illuminating device (6) is located below the culture container (1), and the culture container (1) is made of light-transmitting materials.

10. The microalgae cultivation apparatus as claimed in claim 1, wherein: the temperature adjusting device is characterized in that a temperature adjusting component is arranged in the culture container (1), an external control console is coupled with the temperature adjusting component, and the external control console sends a control instruction to control the temperature adjusting component to release the specified temperature to the culture container (1).