CN102559477A - Thin film photo-bioreactor used for culturing microalgae in industrialization manner - Google Patents

Thin film photo-bioreactor used for culturing microalgae in industrialization manner Download PDF

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Publication number
CN102559477A
CN102559477A CN2011103873097A CN201110387309A CN102559477A CN 102559477 A CN102559477 A CN 102559477A CN 2011103873097 A CN2011103873097 A CN 2011103873097A CN 201110387309 A CN201110387309 A CN 201110387309A CN 102559477 A CN102559477 A CN 102559477A
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film
reactor
opening
algae
algae liquid
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邱志荣
马忠岩
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ZHENJIANG LVNENG ENVIRONMENTAL PROTECTION TECHNOLOGY CO LTD
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ZHENJIANG LVNENG ENVIRONMENTAL PROTECTION TECHNOLOGY CO LTD
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a device and a method for culturing microalgae in a large scale, in particular to a cylinder-shaped thin film reactor device for fast culturing the microalgae. In the cylinder-shaped thin film reactor device, a main body is a vertical steel structure cylinder-shaped or polygonal cylinder bracket, the upper end and the lower end of the bracket are formed by a plurality of stainless steel metal straps arranged in a circular shape through welding, and the cylinder-shaped or polygonal cylinder bracket is arranged in a rectangular formation or a square formation. The lower end of the cylinder-shaped thin film reactor is opened, a pipe is arranged at the position of an opening, the pipe and a thin film are integrated to be a closed structure, the pipe is of a screw structure, the reactor is directly sleeved on the base of the cylinder-shaped bracket, and the upper end of the reactor is provided with an exhaust port, a feed port and an inlet port. A top cover of the reactor is installed and fixed at the upper end of the cylinder-shaped bracket so that direct impact of rainwater, and the like and attachment of dust can be avoided. The base of the cylinder-shaped bracket is provided with the opening, the opening is of the screw structure and can be connected with the pipe of the opening at the lower end of the thin film reactor, and raw material belts are filled in connection position of the opening and the pipe for closing.

Description

A kind of film light bio-reactor that is used for the industrialization cultivating microalgae
Technical field
The present invention relates to be used for the tubular film light bioreactor system that little algae is cultivated in the biological technical field, particularly relate to and utilize power plant's waste gas and waste heat, adopt the LED energy-saving illuminator to be used for the apparatus and method for of industrialization culturing microalgae.
Background technology
Contain dust, CO, CO in power plant's waste gas 2, objectionable impurities such as sulfide, be discharged into arbitrarily and can cause environmental pollution in the atmosphere, also can aggravate Greenhouse effect simultaneously.And the little algae of autotrophic type can be with CO 2Carry out product such as protein, the VITAMINs etc. that photosynthesis produces multiple high added value for carbon source, the algae that some fat contents are high also can be used for the production biofuel.Therefore, if can be with the CO in the waste gas 2Recovery is used for the cultivation of little algae and cultures the dual value that will have economy and environmental protection.
Open bioreactor culturing process receives natural environment influences such as illumination, temperature bigger; And be prone to polluted by fungi, protozoon and other algae kinds; Simultaneously moisture evaporation is serious, the carbonic acid gas undersupply, and these factors will cause all that the frustule culture density is on the low side, the cost of gathering is higher.Closed photosynthetic organism reactor drum culture condition is stable, but high-density culture is carried out in aseptic technique easily, has become developing direction from now on.Little algae algae kind is different, and its growth conditions is different, some algae kind suspension growth in nutrient solution, and some then can be deposited to the nutrient solution bottom.In addition, illumination is a significant effects factor in little algae is cultivated, and therefore, how in the high-density culture of little algae, to guarantee CO easily and effectively 2With the thorough mixing of algae liquid and circulating of algae liquid, necessity of supply simultaneously and competent illumination are problem demanding prompt solutions.
Summary of the invention
1, technical problem to be solved:
Utilize the exhaust gas emission of power plant, make CO in the waste gas 2Gas recycling makes that simultaneously algae kind growth conditions is controlled in the overall optical reactor drum, has realized the high-density culture of algae kind, has both reduced energy consumption, has embodied environmental protection again.
2, technical scheme:
The invention provides a kind of film light bio-reactor that is used for the industrialization cultivating microalgae, comprise Vestolen PP 7052 (PP) film 1, through metal 13 and base 4 thereof, the sample introduction path 5 of reactor drum, the CO of reactor drum 2Air feed path 6, gas drain passageway 12, reactor drum algae liquid is collected path 7; It is characterized in that: Vestolen PP 7052 (PP) film of vertically placing 1 is positioned over the reaction vessel of cultivating as algae liquid in the metal-coating 2, is placed in the open vertical placed metal support 13 described Vestolen PP 7052 (PP) film 1 lower ending opening; And connecting band spiral vessel; Conduit and Vestolen PP 7052 (PP) film 1 is the closed structure of one, and base 4 has an opening to collect outlet ligation device algae liquid collection path 7 for algae liquid, and this opening is that helicitic texture can closely be connected with the film lower ending opening; The sample introduction path 5 of described reactor drum, the CO of reactor drum 212, three paths of air feed path 6 and gas drain passageway close up by the unique opening in film reactor upper end and get into, and pipeline and opening junction are airtight with sealing film, and each path pipeline is separately installed with opening-closing valve.
3, beneficial effect:
Reduce on the one hand the exhaust gas emission of power plant, made CO in the waste gas 2Gas recycling makes that simultaneously algae kind growth conditions is controlled in the overall optical reactor drum, has realized the high-density culture of algae kind, has both reduced energy consumption, has embodied environmental protection again.
Description of drawings
Fig. 1 reactor unit synoptic diagram.
Embodiment
A kind of film light bio-reactor that is used for the industrialization cultivating microalgae comprises Vestolen PP 7052 (PP) film 1, through metal 13 and base 4 thereof, the sample introduction path 5 of reactor drum, the CO of reactor drum 2Air feed path 6, gas drain passageway 12, reactor drum algae liquid is collected path 7; It is characterized in that: Vestolen PP 7052 (PP) film of vertically placing 1 is positioned over the reaction vessel of cultivating as algae liquid in the metal-coating 2, is placed in the open vertical placed metal support 13 described Vestolen PP 7052 (PP) film 1 lower ending opening; And connecting band spiral vessel; Conduit and Vestolen PP 7052 (PP) film 1 is the closed structure of one, and base 4 has an opening to collect outlet ligation device algae liquid collection path 7 for algae liquid, and this opening is that helicitic texture can closely be connected with the film lower ending opening; The sample introduction path 5 of described reactor drum, the CO of reactor drum 212, three paths of air feed path 6 and gas drain passageway close up by the unique opening in film reactor upper end and get into, and pipeline and opening junction are airtight with sealing film, and each path pipeline is separately installed with opening-closing valve.
Vestolen PP 7052 (PP) film 1 lower ending opening; The upper end is semiclosed and to be added with fastener 3 fixing; The spiral vessel of lower ending opening can directly closely be connected with the base of through metal 13; Vestolen PP 7052 (PP) film 1 integral body is vertically placed on the inside of through metal, and film height and diameter can be made corresponding change as required.
Through metal 13 open upper end; Lower end and base 4 directly are welded as a whole, and arrange a week by several metal strip welding between through metal 13 upper and lower surfaces, and the base 4 and the support of through metal 13 are structure as a whole; Base has an opening to collect outlet for algae liquid; This opening is that female thread structure can closely be connected with the film lower ending opening, twines with raw material band between the two, is used for airtight.
Vestolen PP 7052 (PP) film (1) is plastics tubular or polyhedron column.
It is poor that Vestolen PP 7052 (PP) film (1) and through metal 13 tops are reserved with certain altitude.
Also be provided with external light source and additional light source, described external light source adopts the plane electricity-saving lamp, and light source is positioned at the top of axial reactor drum, and the height of light source and quantity can be done corresponding adjustment according to height for reactor.Described additional light source adopts the LED electricity-saving lamp, and the LED electricity-saving lamp is arranged in the formation between any two of reactor drum, also can be built in reactor drum.
A plurality of reactors in series are used the little algae cultivation that is applicable to different scales with the film light biochemical reactor unit of industrialization cultivating microalgae with being together in parallel to form.
A kind of film light biochemical reactor of industrialization cultivating microalgae that utilizes is used for little algae cultivation, comprises following steps:
One. after all parts of said device are installed, close all valves, open algae liquid sample introduction tube valve (8); Inject the chlorine water of about 25mg/l, make chlorine water be full of whole film reactor (1), chlorine water is deposited more than the 24h in film reactor; Behind the 24h; Open algae collection valve (10), close after the emptying chlorine water, use with film reactor of distilled water flushing again.Open algae collection valve (10) once more, close behind the emptying zero(ppm) water;
Two. open nutrient solution input tube valve (8); The volume that injects algae liquid is about 90 ﹪ of whole film reactor, closes nutrient solution input tube valve then, opens algae liquid sample introduction tube valve; Inject the algae liquid of logarithmic phase; Inject long-pending 5 ﹪ that are about the nutrient solution volume of algae liquid, close algae liquid sample introduction pipe (8) then, open CO 2Inlet pipe valve (9) feeds through the CO after the stack gas filtration 2, CO 2Getting into the aeration tube ejection through inlet pipe mixes with algae liquid;
Three. cultivate after 7 ~ 10 days, open algae liquid collection tube valve, utilize microscope under cell counting count board, to count after the sampling, when cell count is higher, open the algae collection and gather.
Culturing with the grid algae is example, adopts the test grade scale, and the access volume ratio is 1% grid algae in the reactor drum of 30L, and the salts solution prescription is following: NaNO3 45g; K2HPO4 .3H20 1.2g; MgSO4 7H2O 2.25g; CaCl2 2H2O 1.08g; Na2CO3 0.6g; Hydrocerol A 0.18g; Ferric ammonium citrate 0.18g; EDTA 2Na 0.03g; MnCl2 4H2O 0.1086g; ZnSO4 7H20 0.01332g; NaMoO4 5H2O 0.0234g; CuSO4 5H2O 0.00474g; H3BO3 0,1776g; Co (NO3) 26H2O 0.002964g; Culture condition is provided with as follows:
Employing power is that 2 watts cold-cathode tube (CCFL) is a light source, and light intensity is 1500 lx, and the alternation of light and darkness time is illumination in 16 hours, 8 hours dark, CO 2Air flow 5% (volume ratio of duty gas).The initial concentration of grid frustule is 4.67 * 10 6Individual/ml, can reach the harvesting time of little algae in 15 days, cell concn reaches 6.78 * 10 7Individual/ml, than significantly having shortened about 7-10 days the harvesting time under ordinary incandescent lamp (power the is 20 watts) illumination condition, living weight also has significantly increases (5.16 * 10 7Individual/ml).The biomass increment of (20w incandescent light) is about 2.11 * 10 under the common illumination condition 6Individual/day, dry weight increase is about 35.28 g m -2d -1,The experimental group biomass increment is about 3.43 * 10 6Individual/day, dry weight increase is about 53.16g m -2d -1,, contrast obviously explains that the grid algae has certain preference to the light source luminescent wavelength, and is more energy-conservation relatively.
Owing to adopted described technical scheme, reduced the exhaust gas emission of power plant on the one hand, make CO in the waste gas 2Gas recycling makes that simultaneously algae kind growth conditions is controlled in the overall optical reactor drum, has realized the high-density culture of algae kind, has both reduced energy consumption, has embodied environmental protection again.Film tubular reactor drum used in the present invention adopts cheap Vestolen PP 7052 (PP) material, has reduced the production cost that little algae is cultivated.
Though the present invention with preferred embodiment openly as above; But they be not be used for limiting of the present invention; Anyly have the knack of this art; In spirit that does not break away from the present invention and scope, certainly when can doing various variations or retouching, so being as the criterion of should being defined with the application's claim protection domain of protection scope of the present invention.

Claims (9)

1. a film light bio-reactor that is used for the industrialization cultivating microalgae comprises Vestolen PP 7052 (PP) film (1), the through metal (13) of Vestolen PP 7052 (PP) film (1) and base (4) thereof, the sample introduction path (5) of reactor drum, the CO of reactor drum 2Air feed path (6); Gas drain passageway (12), reactor drum algae liquid is collected path (7), it is characterized in that: Vestolen PP 7052 (PP) film of vertically placing (1) is positioned over the reaction vessel of cultivating as algae liquid in the metal-coating (2); Be placed in the open vertical placed metal support (13); Described Vestolen PP 7052 (PP) film (1) lower ending opening, and connecting band spiral vessel, conduit and Vestolen PP 7052 (PP) film (1) is the closed structure of one; Base (4) has an opening to collect outlet ligation device algae liquid collection path (7) for algae liquid; This opening is that helicitic texture can closely be connected with the film lower ending opening, the sample introduction path (5) of described reactor drum, the CO of reactor drum 2Air feed path (6) and gas drain passageway (12), three paths close up by the unique opening in film reactor upper end and get into, and pipeline and opening junction are airtight with sealing film, and each path pipeline is separately installed with opening-closing valve.
2. the film light bio-reactor that is used for the industrialization cultivating microalgae as claimed in claim 1; It is characterized in that: described Vestolen PP 7052 (PP) film (1) lower ending opening; The upper end is semiclosed and to be added with fastener (3) fixing; The spiral vessel of lower ending opening can directly closely be connected with the base of through metal (13), and Vestolen PP 7052 (PP) film (1) integral body is vertically placed on the inside of through metal, and film height and diameter can be made corresponding change as required.
3. the film light bio-reactor that is used for the industrialization cultivating microalgae as claimed in claim 1; It is characterized in that: described through metal (13) open upper end; Lower end and base (4) directly are welded as a whole, and arrange a week by several metal strip welding between through metal (13) upper and lower surfaces, and the base (4) of through metal (13) is structure as a whole with support; Base has an opening to collect outlet for algae liquid; This opening is that female thread structure can closely be connected with the film lower ending opening, twines with raw material band between the two, is used for airtight.
4. the film light bio-reactor that is used for the industrialization cultivating microalgae as claimed in claim 1 is characterized in that: it is poor that described Vestolen PP 7052 (PP) film (1) and through metal (13) top are reserved with certain altitude.
5. like the described film light bio-reactor that is used for the industrialization cultivating microalgae of the arbitrary claim of claim 1-4, it is characterized in that: Vestolen PP 7052 (PP) film (1) is plastics tubular or polyhedron column.
6. like the described film light bio-reactor that is used for the industrialization cultivating microalgae of the arbitrary claim of claim 1-4; It is characterized in that: be provided with external light source; Described external light source adopts the plane electricity-saving lamp; Light source is positioned at the top of axial reactor drum, and the height of light source and quantity can be done corresponding adjustment according to height for reactor.
7. like the described film light bio-reactor that is used for the industrialization cultivating microalgae of the arbitrary claim of claim 1-4; It is characterized in that: be provided with additional light source; Described additional light source adopts the LED electricity-saving lamp, and the LED electricity-saving lamp is arranged in the formation between any two of reactor drum, also can be built in reactor drum.
One kind with the film light biochemical reactor unit of industrialization cultivating microalgae, it is characterized in that: a plurality of reactors in series are formed the little algae that is applicable to different scales and cultivated with being together in parallel.
9. one kind is utilized the film light biochemical reactor of industrialization cultivating microalgae to be used for little algae cultivation, comprises following steps:
One. after all parts of said device are installed, close all valves, open algae liquid sample introduction tube valve (8); Inject the chlorine water of about 25mg/l, make chlorine water be full of whole film reactor (1), chlorine water is deposited more than the 24h in film reactor; Behind the 24h, open algae collection valve (10), close after the emptying chlorine water; With with film reactor of distilled water flushing, open algae collection valve (10) once more again, close behind the emptying zero(ppm) water;
Two. open nutrient solution input tube valve (8); The volume that injects algae liquid is about 90 ﹪ of whole film reactor, closes nutrient solution input tube valve then, opens algae liquid sample introduction tube valve; Inject the algae liquid of logarithmic phase; Inject long-pending 5 ﹪ that are about the nutrient solution volume of algae liquid, close algae liquid sample introduction pipe (8) then, open CO 2Inlet pipe valve (9) feeds through the CO after the stack gas filtration 2, CO 2Getting into the aeration tube ejection through inlet pipe mixes with algae liquid;
Three. cultivate after 7 ~ 10 days, open algae liquid collection tube valve, utilize microscope under cell counting count board, to count after the sampling, when cell count is higher, open the algae collection and gather.
CN2011103873097A 2011-11-30 2011-11-30 Thin film photo-bioreactor used for culturing microalgae in industrialization manner Pending CN102559477A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103865758A (en) * 2012-12-13 2014-06-18 财团法人工业技术研究院 Modular photosynthetic bioreactor system, algae cultivation method and clamp group
CN115159771A (en) * 2022-06-16 2022-10-11 科盛环保科技股份有限公司 Water environment restoration device and process

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101709262A (en) * 2009-12-10 2010-05-19 中国科学院广州能源研究所 Solar spectral photosynthetic bioreactor system for culturing microalgae in high density
CN101748054A (en) * 2008-12-17 2010-06-23 新奥科技发展有限公司 Photobioreactor for cultivating microalgae
CN101914431A (en) * 2010-07-23 2010-12-15 江苏省海洋水产研究所 Device and method for cultivating microalgae by utilizing all plastic modular photobioreactor system
CN202369581U (en) * 2011-11-30 2012-08-08 镇江绿能环保科技有限公司 Film bioreactor and reactor unit for industrialized breeding of microalgae

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101748054A (en) * 2008-12-17 2010-06-23 新奥科技发展有限公司 Photobioreactor for cultivating microalgae
CN101709262A (en) * 2009-12-10 2010-05-19 中国科学院广州能源研究所 Solar spectral photosynthetic bioreactor system for culturing microalgae in high density
CN101914431A (en) * 2010-07-23 2010-12-15 江苏省海洋水产研究所 Device and method for cultivating microalgae by utilizing all plastic modular photobioreactor system
CN202369581U (en) * 2011-11-30 2012-08-08 镇江绿能环保科技有限公司 Film bioreactor and reactor unit for industrialized breeding of microalgae

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103865758A (en) * 2012-12-13 2014-06-18 财团法人工业技术研究院 Modular photosynthetic bioreactor system, algae cultivation method and clamp group
CN115159771A (en) * 2022-06-16 2022-10-11 科盛环保科技股份有限公司 Water environment restoration device and process
CN115159771B (en) * 2022-06-16 2024-02-27 科盛环保科技股份有限公司 Water environment restoration device and technology

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Application publication date: 20120711