CN209572543U - A kind of collection of root exudates original position and nearly rhizosphere soil divide root device - Google Patents
A kind of collection of root exudates original position and nearly rhizosphere soil divide root device Download PDFInfo
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- CN209572543U CN209572543U CN201821959139.9U CN201821959139U CN209572543U CN 209572543 U CN209572543 U CN 209572543U CN 201821959139 U CN201821959139 U CN 201821959139U CN 209572543 U CN209572543 U CN 209572543U
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Abstract
It is collected in situ the utility model discloses a kind of root exudates and nearly rhizosphere soil divides root device, including root box, activity is divided to take native box, soil collection inserted sheet and plant unit；Divide among root box and be provided with demarcation plate, activity takes native box for left and right two, and movable to be mounted on a point two sides for the demarcation plate of root box, activity takes native box to be internally provided with slot for being inserted into soil collection inserted sheet；Setting is fluted for fixing plant unit at the top of the demarcation plate, and plant is made to be located at the center for dividing root box.The present apparatus is suitable for studying the response of the root exudates and rhizosphere soil microorganism of plant when root system is by biology/abiotic stress or absorbs Different Nutrients, it designs rigorous, easy to use, it is that one kind can collect plant and divide root culture in the soil, Root morphology observation, root exudates are collected in situ and away from functions such as root different distance pedotheque collections in the plant cultivation device of one.
Description
Technical field
The utility model belongs to plant culture equipment technical field, is related to a kind of collection of root exudates original position and nearly rhizosphere
Soil divides root device, and specially a kind of plant divides root culture in the soil, collecting root exudate in situ and nearly rhizosphere soil
Device.
Background technique
Culture experiment is placed in somatomedin in special container, in the facilities such as greenhouse, solarium or phjytotron
The plant culture test carried out under manual simulation or artificial control condition.Since it is with strict control moisture, nutrient, temperature
Environmental conditions such as degree, illumination and the characteristics of avoid the uncontrollable environmental factor in crop field, and it is widely used in the life of plant simulation cultivation
It manages Biochemical Mechanism research and explores, be that the normal of current Plant Nutrition, Plant Pathology and corresponding plants ambit is tested
Method.
Currently, the plant soil-borne diseases regulation and prevention and treatment and Plant Nutrition and soil microbiology of plant pathology subject
Concern of the crossing research increasingly by vast researcher.Common soil-borne disease has root rot, wilt disease and gray mold
It is typically all to be entered in plant by the pathogen infection root system of plant in soil Deng, Pathogenic pathway, the growth generation of pathogen
The activity of thanking and its certain metabolites damage the histoorgan of plant, so as to cause the various physiological and biochemical procedures of plant
Change.Pathogen is widely present with soil environment, and the pathogen in general in soil-borne disease has the special of height to plant
One property, long-term continuous tillage method can lead to the enrichment of pathogen and soil microbial community in soil in agricultural system
Acute variation, thus increase soil-borne disease generation probability and degree.
Live in concentrated communities a large amount of microorganisms in the soil that plant depends on for existence, including bacterium, fungi, actinomyces, algae, primary dynamic
Object and virus etc., Soil Microorganism are influenced in colonizing for rhizosphere by root exudates, and apparent rhizosphere effect is shown.Root
It is the important medium that secretion is plant and Soil Micro-environment progress substance, energy and information interchange, is to maintain root-zone soil
The important component of material transport and adjusting in the key factor and rhizosphere microecological system of system vigor.Numerous studies
Show that rhizosphere microorganism structure of community and soil-borne disease have certain inner link, the generation of soil-borne disease is certain
Be in degree as rhizosphere microorganism structure of community it is unbalance caused by.Root exudates has direct shadow to rhizosphere microorganism activity
Sound, the formation of specified plant rhizosphere microflora and the component of root exudates and quantity close relation.Root system secretion
Object is not only the energy needed for rhizosphere microorganism provides growth, and can influence the quantity and population structure of rhizosphere microorganism,
And metabolism, growth and development and the diversity of rhizosphere microorganism, to influence the generation of disease.It is tested in traditional soil incubation
In, since root system of plant free growth is in soil media, there are technical difficulty to be collected to the in situ of root exudates, therefore,
Develop a kind of plant and divide root culture in the soil, can with the device of collecting root exudate in situ, to root system of plant regulation and
Divide root experimental study that there is important theoretical value and application prospect.On this basis, by away from root different distance soil-like
The collection of product, the important meaning that research plant has in edaphon subject with the soil microbial community in microbial interaction
Justice.
Utility model content
Purpose of utility model: the purpose of this utility model is that in view of the deficiencies of the prior art, providing a kind of root system secretion
Object is collected in situ and nearly rhizosphere soil divides root device, which is suitable for studying root exudates and the micro- life of rhizosphere soil of plant
Response of the object when root system is by biology/abiotic stress or absorbs Different Nutrients, and design rigorous, easy to use, it is
One kind can collect plant and divide root culture in the soil, Root morphology observation, root exudates collect in situ and away from root difference away from
From functions such as pedotheque collections in the plant cultivation device of one.
Technical solution: to achieve the goals above, such as a kind of root exudates original position collection described in the utility model and closely
Rhizosphere soil divides root device, including root box, activity is divided to take native box, soil collection inserted sheet and plant unit；Among described point of root box
It is provided with demarcation plate, the activity takes native box for left and right two, and movable to be mounted on a point two sides for the demarcation plate of root box, activity takes
Native box is internally provided with slot for being inserted into soil collection inserted sheet；Setting is fluted for fixing plant list at the top of the demarcation plate
Member makes plant be located at the center for dividing root box.
Wherein, described to divide root box generally cuboid, it is opened wide above uncovered, centre is divided into identical two with demarcation plate
A region.
Wherein, the activity takes native box generally cuboid, and top and left/right side uncovered open wide, unlimited side nylon
Net package.
Wherein, the slot is arranged in activity and takes on the inside of native box and close to the unlimited place of nylon wire package, and each activity takes
1-10 slot is provided in native box.It is usually provided with 5 slots.
Wherein, the plant unit includes plant shoot, intermediate sponge ring and the plant left side for being divided into the left and right sides
Lateral root system, right side root system.
Preferably, the sponge ring is wrapped in plant basal part of stem, fill in the groove of demarcation plate, fixed plant unit.
Wherein, described to divide that root box, activity take native box and demarcation plate is organic glass.
The soil collection inserted sheet be stainless steel substrates, lower end polishing it is sharp, thickness is thin compared with other positions of inserted sheet, convenient for according to
The track reeve of slot, which divides, carries out collecting soil sample in the soil in root box.
Plant used in plant unit is rice, cucumber, wheat, one or more of corn etc. in the utility model.It plants
The plant of object unit needs the first preculture in water planting or vermiculite class matrix for a period of time, turns after root growth to certain volume
It moves in point root device.When plant unit is transferred to point root device, root system of plant needs to remove on main root and by lateral root average mark
For the left and right sides.
Further, soil media used by the utility model is conventional soil, cause of disease soil, sterile soil etc., soil
The left and right lateral root of nylon wire and plant unit that earth needs the full left and right sides activity of equivalent filling to take native box, and take native box for two
System is close to respectively.
Working principle: first root box will be divided to take native box clean clearly with activity when use, nylon wire is blocked with double-sided adhesive and is taken
The open side of native box is fixed on point groove in root box center using sponge ring after then dividing equally root system of plant seedlings or so
In, the root system of left and right sides is adjacent to the partition in root box center is divided, the small-sized native box that takes for being put into two sides, keeps demarcation plate, left and right sides
Root system and nylon wire three are adjacent to, then soil or culture substrate are added in native box to taking for two sides, and supply moisture and support
Divide progress plant culture, when testing completion, the careful native box that takes for taking out two sides removes nylon wire, will acquire inserted sheet according to slotting
The track of slot injects in the soil taken in native box and carries out collecting soil sample, and the pedotheque of different distance is retained separately,
Finally the left and right root system of plant two sides is carefully removed with demarcation plate, and plant holding original state is transferred to and new divides root box
In, sterile ultrapure water is added to two sides and carries out root exudates collection.
The utility model has the advantages that compared with prior art, the utility model has the advantages that
Utility model device design is rigorous, easy to use, can be realized plant and divides root soil incubation, Root morphology is seen
Survey, root exudates in situ collect and away from root different distance pedotheque collect etc. functions, can be widely used for plant physiology,
The related sciences such as Plant Pathology and soil microbiology field.
Root system of plant and soil separate but not influenced between root system and soil by the nylon wire of the utility model
Mass exchange, can be used for being similar to soil incubation root exudates in situ collect；Using slot and soil collection inserted sheet
It is separately collected using by the anthropogenic soil apart from root system different distance, can be used for studying plant to the shadow of Soil biochemical property
It rings.
The root exudates of the utility model is collected in situ and nearly rhizosphere soil point root device can be used for studying plant roots
The response of root exudates, rhizosphere metabolin and rhizosphere microorganism when being by biotic (Mycophyta pathogen)；Research
The response of root exudates, rhizosphere metabolin and rhizosphere microorganism when root system of plant is by abiotic stress (arid)；And
The sound of root exudates, rhizosphere metabolin and rhizosphere microorganism when researching plant root absorbs Different Nutrients (high nitrogen and low nitrogen)
It should wait；Using simplicity, effect is obvious, significant effect.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model.
Specific embodiment
Below in conjunction with drawings and examples, the utility model is described in further detail.
Embodiment 1
As shown in Figure 1, a kind of root exudates is collected in situ and nearly rhizosphere soil divides root device, including divide root box 1, activity
Take native box 2, soil collection inserted sheet 3 and plant unit；It is provided with demarcation plate 4 among described point of root box 1, divides root box 1 generally long
Cube is made by organic glass, is opened wide above uncovered, long 30cm, wide 10cm, high 20cm, thickness 0.2cm, centre demarcation plate 4
It is divided into identical two regions, demarcation plate 4 is organic glass production, and shape is rectangle, and top is provided with rectangular recess
6, demarcation plate 4 is 18.8cm long, wide 9.6cm, and groove 6 is 2cm long, wide 1cm.
Activity takes native box 2 for left and right two, and movable to be mounted on a point two sides for the demarcation plate of root box 14, activity takes native box 2
Generally cuboid, long 13cm, wide 8.6cm, high 22cm, thickness 0.5cm, top and left/right side uncovered open wide, and open wide side and use
Nylon wire 7 wraps up.Activity takes native box 2 to be internally provided with slot 5 for being inserted into soil collection inserted sheet 3；The setting of slot 5 takes in activity
The unlimited place that native 2 inside of box and close nylon wire 7 wrap up, each activity, which takes, is provided with 5 slots 5 in native box 2, can be with 1-10
Setting is chosen in slot as needed, slot 5 is symmetrically provided on the inside of the front and rear sides box body that left/right opens wide place 0.1cm
First of slot of width 0.15cm, depth 0.2cm, length is 21.5cm from top to bottom, by the inside 0.1cm of first of slot
It is provided with identical second slot, and so on, there are 5 slots altogether.
4 top groove 6 of demarcation plate is used for the fixation of plant unit, and plant is made to be located at the center for dividing root box 1.Plant unit packet
Include plant shoot 8, intermediate sponge ring 9 and the plant left side root system 10, right side root system 11 that are divided into the left and right sides.
Sponge ring 9 for fixing is wrapped in plant basal part of stem, fills in the groove 6 of demarcation plate 4, fixed plant unit.
Soil collection inserted sheet 3 is stainless steel substrates, and lower end polishing is sharp, and thickness is thin compared with other positions of inserted sheet.
It will first divide root box 1 and activity to take native box 2 clear clean when use, nylon wire 7 is blocked with double-sided adhesive and takes native box
Open side is fixed on a point root using sponge ring 9 after then dividing equally the left side root system 10 of the root system of plant seedlings, right side root system 11
In the groove 6 in box center, left side root system 10, right side root system 11 are adjacent to the partition 4 in root box center is divided, the small-sized work for being put into two sides
It is dynamic to take native box 2, keep demarcation plate 4, root system and 7 three of nylon wire to be adjacent to, then soil or training are added in native box 2 to taking for two sides
Matrix is supported, and supplies moisture and nutrient progress plant culture according to experimental design, when testing completion, careful taking-up two sides
Take native box 2, remove nylon wire 7, by soil collection inserted sheet 3 according to the track reeve activity of slot 5 take in the soil in native box 2 into
Row collecting soil sample, and the pedotheque of different distance is retained separately, finally by the left side root system 10 of plant two sides, right side
Root system 11 carefully with demarcation plate 4 remove, and by plant holding original state be transferred to it is new divide in root box 1, to two sides be added it is sterile
Ultrapure water carries out root exudates and collects
Embodiment 2
1, collected in situ using the utility model root exudates and nearly rhizosphere soil divide root device researching plant root by
The response of root exudates, rhizosphere metabolin and rhizosphere microorganism when to biotic (Mycophyta pathogen): by plant children
Root system of seedling or so moves into divide in root box after dividing equally to be started to cultivate, and left and right sides edaphic condition keeps identical (quality, physics knot
Structure, nutrient, moisture), it is transplanting after a week, left side soil inoculation pathogen spore suspension, right side adds the sterile Fluid Dynamics of equivalent, to
Culture after a certain period of time, carefully takes out soil chamber, and acquire different distance rhizosphere soil sample using inserted sheet, is used as rhizosphere and is metabolized
Object and rhizosphere microorganism measurement analysis, by plant whole be transferred to it is new divide in root box, during which keep left and right sides root system point
It opens, ultrapure water is added in root box and collects root exudates 12 hours, it is to be measured after freeze-drying concentration.
2, collected in situ using the utility model root exudates and nearly rhizosphere soil divide root device researching plant root by
The response of root exudates, rhizosphere metabolin and rhizosphere microorganism when to abiotic stress (arid): by the root of plant seedlings
System moves into after dividing equally left and right to be divided in root box and starts to cultivate, left and right sides edaphic condition keep it is identical (quality, physical structure, nutrient,
Moisture), in transplanting after a week by the irrigation amount of control left and right sides, so that left side soil moisture content is maintained 10% or so, right side
Soil moisture content maintains 25% or so, careful to take out soil chamber and acquired not using inserted sheet wait continue culture after a certain period of time
Same distance rhizosphere soil sample is used as rhizosphere metabolin and rhizosphere microorganism measurement analysis, plant whole is transferred to new
Divide in root box, left and right sides root system is during which kept to separate, ultrapure water collection root exudates 12 hours, freezing are added in root box
It is to be measured after dry concentration.
3, it is collected in situ using the utility model root exudates and nearly rhizosphere soil divides root device researching plant root to inhale
The response of root exudates, rhizosphere metabolin and rhizosphere microorganism when receiving Different Nutrients (high nitrogen and low nitrogen): by plant seedlings
Root system or so divide equally after move into divide in root box and start to cultivate, left and right sides edaphic condition keep it is identical (quality, physical structure,
Non-nitrogen nutrient, moisture), the nitrogenous fertilizer of a large amount level is used in the soil of left side, right side soil applies the nitrogenous fertilizer for being reduced half, wait cultivate one
After fixing time, careful soil chamber of taking out simultaneously acquires different distance rhizosphere soil sample using inserted sheet, be used as rhizosphere metabolin and
Rhizosphere microorganism measurement analysis, by plant whole be transferred to it is new divide in root box, during which keep the left and right sides root system separate, in root
Ultrapure water is added in box to collect root exudates 12 hours, it is to be measured after freeze-drying concentration.
Claims (9)
1. a kind of root exudates is collected in situ and nearly rhizosphere soil divides root device, which is characterized in that including dividing root box (1), living
It is dynamic to take native box (2), soil collection inserted sheet (3) and plant unit；Demarcation plate (4), the work are provided among described point of root box (1)
Dynamic to take native box (2) for left and right two, the two sides of the movable demarcation plate (4) for being mounted on point root box (1), activity takes in native box (2)
Portion is provided with slot (5) for being inserted into soil collection inserted sheet (3)；Fluted (6) are set at the top of the demarcation plate (4) for fixing
Plant unit makes plant be located at the center for dividing root box (1).
2. root exudates according to claim 1 is collected in situ and nearly rhizosphere soil divides root device, which is characterized in that institute
Point root box (1) generally cuboid is stated, is opened wide above uncovered, centre is divided into identical two regions with demarcation plate (4).
3. root exudates according to claim 1 is collected in situ and nearly rhizosphere soil divides root device, which is characterized in that institute
The activity of stating takes native box (2) generally cuboid, and top and left/right side uncovered open wide, and opens wide side and is wrapped up with nylon wire (7).
4. root exudates according to claim 3 is collected in situ and nearly rhizosphere soil divides root device, which is characterized in that institute
It states slot (5) setting to take in activity on the inside of native box (2) and close to the unlimited place of nylon wire (7) package, each activity takes native box (2)
Inside it is provided with 1-10 slot (5).
5. root exudates according to claim 1 is collected in situ and nearly rhizosphere soil divides root device, which is characterized in that institute
State soil collection inserted sheet (3) lower end polishing it is sharp, thickness is thin compared with other positions of inserted sheet.
6. root exudates according to claim 1 is collected in situ and nearly rhizosphere soil divides root device, which is characterized in that institute
Stating plant unit includes plant shoot (8), intermediate sponge ring (9) and the plant left side root system for being divided into the left and right sides
(10), right side root system (11).
7. root exudates according to claim 6 is collected in situ and nearly rhizosphere soil divides root device, which is characterized in that institute
It states sponge ring (9) and is wrapped in plant basal part of stem, fill in the groove (6) of demarcation plate (4), fixed plant unit.
8. root exudates according to claim 1 is collected in situ and nearly rhizosphere soil divides root device, which is characterized in that institute
State a point root box (1), activity takes native box (2) and demarcation plate (4) is organic glass.
9. root exudates according to claim 1 is collected in situ and nearly rhizosphere soil divides root device, which is characterized in that institute
Stating soil collection inserted sheet (3) is stainless steel substrates.
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| CN201821959139.9U CN209572543U (en) | 2018-11-26 | 2018-11-26 | A kind of collection of root exudates original position and nearly rhizosphere soil divide root device |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110672351A (en) * | 2019-11-08 | 2020-01-10 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
| CN111011029A (en) * | 2019-12-31 | 2020-04-17 | 中国科学院成都生物研究所 | Plant whole life cycle rhizosphere information acquisition method and system |
| CN111642277A (en) * | 2020-07-08 | 2020-09-11 | 江西省农业科学院土壤肥料与资源环境研究所 | Plant culture device and method for collecting rhizosphere soil |
| CN113170721A (en) * | 2021-04-01 | 2021-07-27 | 沈阳大学 | Method and system for obtaining compound absorbed and transformed by plant without damage |
| CN115316252A (en) * | 2022-10-10 | 2022-11-11 | 中国科学院昆明植物研究所 | Method for establishing root division system in herbaceous root hemiparasitic plant vessel |
-
2018
- 2018-11-26 CN CN201821959139.9U patent/CN209572543U/en active Active
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110672351A (en) * | 2019-11-08 | 2020-01-10 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
| CN110672351B (en) * | 2019-11-08 | 2021-02-02 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
| CN111011029A (en) * | 2019-12-31 | 2020-04-17 | 中国科学院成都生物研究所 | Plant whole life cycle rhizosphere information acquisition method and system |
| CN111011029B (en) * | 2019-12-31 | 2022-10-14 | 中国科学院成都生物研究所 | Plant whole life cycle rhizosphere information acquisition method and system |
| CN111642277A (en) * | 2020-07-08 | 2020-09-11 | 江西省农业科学院土壤肥料与资源环境研究所 | Plant culture device and method for collecting rhizosphere soil |
| CN111642277B (en) * | 2020-07-08 | 2021-12-10 | 江西省农业科学院土壤肥料与资源环境研究所 | Plant culture device and method for collecting rhizosphere soil |
| CN113170721A (en) * | 2021-04-01 | 2021-07-27 | 沈阳大学 | Method and system for obtaining compound absorbed and transformed by plant without damage |
| CN115316252A (en) * | 2022-10-10 | 2022-11-11 | 中国科学院昆明植物研究所 | Method for establishing root division system in herbaceous root hemiparasitic plant vessel |
| CN115316252B (en) * | 2022-10-10 | 2023-01-31 | 中国科学院昆明植物研究所 | Method for establishing root division system in herbaceous root hemiparasitic plant vessel |
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