CN103733862B - Cultivation method for thuja seeds - Google Patents
Cultivation method for thuja seeds Download PDFInfo
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- CN103733862B CN103733862B CN201410014862.XA CN201410014862A CN103733862B CN 103733862 B CN103733862 B CN 103733862B CN 201410014862 A CN201410014862 A CN 201410014862A CN 103733862 B CN103733862 B CN 103733862B
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Abstract
The invention discloses a cultivation method for thuja seeds. The cultivation method includes the following steps: a, in the mid-to-late October every year, timely collecting the thuja seeds, picking cones during seed collection, putting the cones in indoor space for natural airing, screening out the seeds, putting the seeds into a ventilated cloth bag to be stored at the normal temperature for later use, and completing sowing before March of the next year; b, picking the thuja seeds, sterilizing the seeds by a HgCl2 (mercury chloride) solution with mass fraction of 1-2% for 5-10 minutes, soaking the seeds in a rooting powder solution with mass fraction of 0.001-0.002% for 8-12 hours, putting the seeds in a cultivation box, and cultivating the seeds at the temperature of 20-25 DEG C in a humid environment for 10-15 days to acquire budding seeds; C, sowing the budding seeds in media, placing the media under a sunshade, spraying mist and watering the seeds, performing routine management to acquire germinant seedlings, transplanting the seedlings to a seedbed with a sunshade after the seedlings grow to 3-5cm, and ensuring the seedlings to grow to acquire thuja seedlings. The soil of the seedbed is mixed soil formed by mixing of humus and river sand according to mass ratio of 1: 1.
Description
Technical field:
The invention belongs to field of plant growing technology, be specifically related to a kind of arborvitae seed propagation method.
Background technology:
Arborvitae Thuja sutchuenensis Franch. shrub or arbor; Branch is close, carries out, and the sprig of raw scale leaf is flat.Leaf squama shape, is born in leaf oblique side's shape obovate of sprig central authorities, bossed longitudinal ridge, some longitudinal ridges have strip groove, long 1.5 ~ 3mm, wide 1.2 ~ 1.5mm, tip is blunt, below without gland point, the leaf ship shape of side, wide lanceolar, more central leaf is slightly short, wide 0.8-1mm, tip is blunt, curved in tip, two sides is green, without white powder.Male cone is closely oval, is about 2.5mm, and stamen about 8 is right, intersects to life, and connective width egg shape, tip is blunt.Immature cone is about 5.5mm, and oval, fruit scale 8, intersects to life, and the outmost fruit scale shape of falling ovum is oval, has a squamous tip below top.
Be born in height above sea level 1400m Limestone Mountain, main product Chongqing City Chengkou County Daba Mountain.Also there is a small amount of distribution nature reserve, calyx mountain, Wanyuan City, Sichuan Province and Wudaoxia Nature Reserve, Baokang city of Hubei Province.The various soil zones of warm and moist are born in happiness, especially with well-grown on Limestone Mountain calcium soil.
Arborvitae is gymnosperm the most rare in the world.Once for the moment flouring in the Cretaceous period, have many types.When transition occur earth climatic environment, a large amount of extinct plants and animal becomes extinct in succession, but arborvitae relies on indomitable vitality to survive, and just population deterioration is serious, becomes " living fossil " species very rare in the world to today.
On April 9th, 1892, the sample obtaining this Plants is adopted at the Limestone Mountain at suitable small stream height above sea level 1400 meters of of the southeast, Chengkou, Chongqing county first by France missionary's Bauer Joe Fargis (R.P.Farges), sample is by Paris National Museum of Natural History collection (sample number: Farges1158) subsequently, becomes the type specimen of novel species.This is known to arborvitae science record the earliest in worldwide.But after this more than 100 year, scientist repeatedly went to the place of production to find, and arborvitae but disappears seemingly without foundation.1998 World Conservation Union (IUCN) arborvitae is classified as the Chinese Endemic and rare species of having died out.But through the travel of difficult searching, within 1999, arborvitae is rediscovered by Chinese Scientists, and developed to the not extinction of whole world declaration arborvitae by gymnosperm association of world chairman A Liusi.To these once " disappearance " seeds in more than 100 year, only there is the research of a small amount of COMMUNITY CHARACTERISTICS and genetic diversity aspect.
Summary of the invention:
The object of the invention is to break through arborvitae seminal propagation obstacle, explore its seed germination mechanism, and provide a kind of easy and simple to handle, with low cost, the arborvitae seed propagation method of good seed can be provided for arborvitae industrialized development.
Arborvitae seed propagation method of the present invention, is characterized in that, comprise the following steps:
A, to collect seed: in annual mid or late October, arborvitae seed of immediately gathering, adopts cone during seed collecting and transfer Indoor Natural and dry in good time, sift out seed and load normal temperature in the cloth bag that ventilates and preserve, for subsequent use, complete sowing before treating March in next year;
B, presprouting of seeds: get arborvitae seed, first through mass fraction 1 ~ 2 ‰ HgCl
2solution disinfection 5-10 minute, then puts into mass fraction 0.001-0.002% rooting powder solution and soaks 8-12h, then be positioned in incubator by seed, under moist environment, cultivation temperature controls at 20 DEG C ~ 25 DEG C, cultivates 10 ~ 15 days, obtain the seed after sprouting, rate 40%-50% of sprouting;
C, sowing and seedling replanting: by the planting seed after sprouting in matrix, under being placed in shading shed, spraying is watered, Routine Management, obtains the seedling germinateed, when seedling grows to 3 ~ 5cm, be transplanted to and have on the seedbed of shading shed, the soil on seedbed is humus and the sandy composite soil mixed according to mass ratio 1:1, growth of seedling, obtain arborvitae seedling, described matrix is by humus soil and the river sand mixed composite soil of 1:1 or humus or river sand or vermiculite in mass ratio.
Preferably, sowing was completed before the March in next year described in step a.This time period is extremely important, because arborvitae seed is along with the prolongation of holding time, germination rate declines rapidly.Described in step c by the planting seed after sprouting in matrix, under being placed in shading shed, the shading rate of the shading screen on shading shed is 70%, and being transplanted to described in step c has on the seedbed of shading shed, and the shading rate of the shading screen on shading shed is 60 ~ 70%.
The present invention determines the best period of arborvitae seeds and sowing, have selected best seeding method and best sowing media, thus achieves arborvitae seminal propagation.Arborvitae seed propagation method of the present invention solves a difficult problem for arborvitae seed natural propagation rate extremely low (about 0.1%), by suitable process, can larger raising arborvitae reproduction speed and emergence rate, not only can to meet in production practices and research work to the portion requirements of seedling, also can solve the problem that vegetative propagation seedling resistance is poor, survival rate is low.The seedling of seminal propagation is healthy and strong, and strong stress resistance, easily tames, and success rate is high, has good ecological benefits, economic benefit and social benefit.The method key technical problems be find arborvitae to sow right times, vernalization measure, technical operation is easy, with low cost, can provide good seed for arborvitae industrialized development.
Embodiment:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1:
A, to collect seed: in Chongqing province of China Chengkou County, annual mid or late October, arborvitae Thuja sutchuenensisFranch seed of immediately gathering, adopts cone during seed collecting and transfer Indoor Natural and dry in good time, sift out seed and load normal temperature in the cloth bag that ventilates and preserve, for subsequent use.
B, to sow in March in next year, adopt quartering to divide and get seed: be on experimental bench, put three blank sheet of paper, centre is a 8K paper, both sides are 16K paper, the arborvitae seed sample of about the 500g in seed packet is poured on 8K paper, and stand rectangularity or square, require that thickness is even, then diagonally arborvitae seed is divided into four triangles with ruler, then from every part, gets 1000 at random as sample.
C, presprouting of seeds: by above-mentioned 4 parts of arborvitae seed samples of taking-up through mass fraction 1 ‰ HgCl
2solution disinfection 10 minutes, then polymer solution in water seed being put into mass fraction 0.001% root-inducing powder soaks 12h, afterwards seed is placed in respectively and is covered with in 4 culture dishes of 2 metafiltration paper, watering, be positioned over afterwards in incubator, cultivation temperature controls at 20 DEG C ~ 25 DEG C again, cultivates 10 days, obtain the seed after sprouting, rate 40 ~ 50% of sprouting.
D, sowing: sowed by the seed after sprouting respectively in 4 kinds of different matrix: a, fill humus soil and river sand (mass ratio 1:1); B, full humus; C, full river sand; D, full vermiculite, matrix is contained in high 50cm respectively, and in the round plastic basin of diameter 40cm, the height of matrix is 40cm.100 seeds are broadcast in each process, if 3 repetitions.Plastic basin put in the shading shed of 70% shading rate, Routine Management, every day, spraying was watered, about 30 days, humus and river sand composite soil were emerged survival rate 20-25%, and humus soil is emerged survival rate 17-20%, sandy survival rate 15-18% of emerging, vermiculite soil is emerged survival rate 13-15%.
E, seedling replanting and management: when the seedling germinateed grows to 3 ~ 5cm, be transplanted on seedbed, nursery soil is humus and sandy composite soil (mass ratio 1:1), frame is built in seedbed, frame is added a cover the shading screen of shade rate 60%; After 7 ~ 10 days, seedling takes root growth, within 2 ~ 3 days, waters once; About 150 days, seedling grew to 10 ~ 15cm, carefully packed, conventional nursery bed management, obtained arborvitae seedling.
Embodiment 2:
A, to collect seed: in Chongqing province of China Chengkou County, annual mid or late October, seed of immediately gathering, adopts cone during seed collecting and transfer Indoor Natural and dry in good time, sift out seed and load normal temperature in the cloth bag that ventilates and preserve, for subsequent use.
B, to sow in March in next year, adopt quartering to divide and get seed: be on experimental bench, put three blank sheet of paper, centre is a 8K paper, both sides are 16K paper, the arborvitae seed sample of about the 500g in seed packet is poured on 8K paper, and stand rectangularity or square, require that thickness is even, then diagonally arborvitae seed is divided into four triangles with ruler, then from every part, gets 1000 at random as sample.
C, presprouting of seeds: by above-mentioned 4 parts of arborvitae seed samples of taking-up through mass fraction 2 ‰ HgCl
2solution disinfection 5 minutes, then polymer solution in water seed being put into mass fraction 0.002% root-inducing powder soaks 8h, afterwards seed is placed in respectively and is covered with in 4 culture dishes of 2 metafiltration paper, watering, be positioned over afterwards in incubator, cultivation temperature controls at 20 DEG C ~ 25 DEG C again, cultivates 15 days, obtain the seed after sprouting, rate 40 ~ 50% of sprouting.
D, sowing: sowed by the seed after sprouting respectively in 4 kinds of different matrix: a, fill humus soil and river sand (mass ratio 1:1); B, full humus; C, full river sand; D, full vermiculite, matrix is contained in high 50cm respectively, and in the round plastic basin of diameter 40cm, the height of matrix is 40cm.100 seeds are broadcast in each process, if 3 repetitions.Plastic basin put in the shading shed of 70% shading rate, Routine Management, every day, spraying was watered, about 30 days, humus and river sand composite soil were emerged survival rate 20-25%, and humus soil is emerged survival rate 17-20%, sandy survival rate 15-18% of emerging, vermiculite soil is emerged survival rate 13-15%.
E, seedling replanting and management: when the seedling germinateed grows to 3 ~ 5cm, be transplanted on seedbed, soil is humus and sandy composite soil (mass ratio 1:1), frame is built in seedbed, frame is added a cover the shading screen of shade rate 70%; After 7 ~ 10 days, seedling takes root growth, within 2 ~ 3 days, waters once; About 150 days, seedling grew to 10 ~ 15cm, carefully packed, conventional nursery bed management, obtained arborvitae seedling.
Claims (2)
1. an arborvitae seed propagation method, is characterized in that, comprises the following steps:
A, to collect seed: in annual mid or late October, arborvitae seed of immediately gathering, adopts cone during seed collecting and transfer Indoor Natural and dry in good time, sift out seed and load normal temperature in the cloth bag that ventilates and preserve, for subsequent use, complete sowing before treating March in next year;
B, presprouting of seeds: get arborvitae seed, first through mass fraction 1 ~ 2 ‰ HgCl
2solution disinfection 5-10 minute, then puts into mass fraction 0.001-0.002% rooting powder solution and soaks 8-12h, then be positioned in incubator by seed, under moist environment, cultivation temperature controls at 20 DEG C ~ 25 DEG C, cultivates 10 ~ 15 days, obtains the seed after sprouting;
C, sowing and seedling replanting: by the planting seed after sprouting in matrix, under being placed in shading shed, spraying is watered, Routine Management, obtains the seedling germinateed, when seedling grows to 3 ~ 5cm, be transplanted to and have on the seedbed of shading shed, the soil on seedbed is humus and the sandy composite soil mixed according to mass ratio 1:1, growth of seedling, obtain arborvitae seedling, described matrix is by humus soil and the river sand mixed composite soil of 1:1 or humus or river sand or vermiculite in mass ratio.
2. arborvitae seed propagation method according to claim 1, it is characterized in that, sowing was completed before March in next year described in step a, this time period is extremely important, because arborvitae seed is along with the prolongation of holding time, germination rate declines rapidly, described in step c by the planting seed after sprouting in matrix, under being placed in shading shed, the shading rate of the shading screen on its shading shed is 70%, being transplanted to described in step c has on the seedbed of shading shed, and the shading rate of the shading screen on shading shed is 60 ~ 70%.
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| CN104871981A (en) * | 2015-07-01 | 2015-09-02 | 临沂大学 | Western red cedar tissue culture rapid propagation method |
| CN107360755A (en) * | 2017-08-09 | 2017-11-21 | 合肥沁牧生态农业有限公司 | A kind of arborvitae method for treating seeds for improving germination percentage |
Citations (4)
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|---|---|---|---|---|
| US4353184A (en) * | 1981-05-18 | 1982-10-12 | Weyerhaeuser Company | Method for asexual reproduction of coniferous trees |
| CN101699946A (en) * | 2009-09-30 | 2010-05-05 | 甘肃省祁连山水源涵养林研究院 | Pregermination method of Sabina Przewalskii kom seeds |
| CN101869027A (en) * | 2009-04-23 | 2010-10-27 | 上海上房园林植物研究所 | Cutting propagation method for orange Thuja |
| CN102960249A (en) * | 2012-11-30 | 2013-03-13 | 通化师范学院 | In-vitro efficient seedling cultivation method synchronous in rooting and growing by utilizing tender stem segments of thuja koraiensis |
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2014
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4353184A (en) * | 1981-05-18 | 1982-10-12 | Weyerhaeuser Company | Method for asexual reproduction of coniferous trees |
| CN101869027A (en) * | 2009-04-23 | 2010-10-27 | 上海上房园林植物研究所 | Cutting propagation method for orange Thuja |
| CN101699946A (en) * | 2009-09-30 | 2010-05-05 | 甘肃省祁连山水源涵养林研究院 | Pregermination method of Sabina Przewalskii kom seeds |
| CN102960249A (en) * | 2012-11-30 | 2013-03-13 | 通化师范学院 | In-vitro efficient seedling cultivation method synchronous in rooting and growing by utilizing tender stem segments of thuja koraiensis |
Non-Patent Citations (2)
| Title |
|---|
| 《崖柏和侧柏幼苗对自然降温的生理生化反应》;朱莉等;《林业科学研究》;20130415;第26卷(第2期);全文 * |
| 《福建柏圃地育苗技术》;侯伯鑫等;《林业实用技术》;20061215(第12期);全文 * |
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