CN211407170U - Water culture device for arabidopsis thaliana - Google Patents
Water culture device for arabidopsis thaliana Download PDFInfo
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- CN211407170U CN211407170U CN201922131168.7U CN201922131168U CN211407170U CN 211407170 U CN211407170 U CN 211407170U CN 201922131168 U CN201922131168 U CN 201922131168U CN 211407170 U CN211407170 U CN 211407170U
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- 241000219195 Arabidopsis thaliana Species 0.000 title claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title description 14
- 235000015097 nutrients Nutrition 0.000 claims abstract description 15
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 13
- 239000003501 hydroponics Substances 0.000 claims description 12
- 239000003755 preservative agent Substances 0.000 claims description 10
- 230000002335 preservative effect Effects 0.000 claims description 10
- 239000004033 plastic Substances 0.000 claims description 6
- 229920003023 plastic Polymers 0.000 claims description 6
- 241000219194 Arabidopsis Species 0.000 claims description 4
- 238000004891 communication Methods 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 12
- 238000000034 method Methods 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 7
- 239000000463 material Substances 0.000 description 5
- 239000002689 soil Substances 0.000 description 5
- 238000012136 culture method Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000004251 balanced diet Nutrition 0.000 description 1
- 230000037208 balanced nutrition Effects 0.000 description 1
- 235000019046 balanced nutrition Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Hydroponics (AREA)
Abstract
The utility model relates to an arabidopsis thaliana hydroponic device, a serial communication port, include: the cultivation box adopts an opaque box body, the top of the cultivation box is open, and nutrient solution is placed inside the cultivation box; the cultivation plate comprises a cultivation plate body horizontally arranged in the cultivation box and a plurality of cultivation cup bodies which are protruded and uniformly distributed at the bottom of the cultivation plate body, and the bottoms of the cultivation cup bodies are open; the shading plate horizontally covers the upper portion of the cultivation plate, and a plurality of through holes communicated with the cultivation cup bodies in a one-to-one correspondence mode are formed in the shading plate.
Description
Technical Field
The utility model relates to a hydroponic device especially relates to an arabidopsis thaliana hydroponic device, belongs to soilless culture technical field.
Background
The arabidopsis thaliana is used as an important model plant for molecular genetics research, and different planting modes lay a foundation for the deep research of the arabidopsis thaliana. Hydroponics (Hydroponics) is a novel soilless culture mode of plants, also called nutrient solution culture, and is characterized in that root systems of the plants are directly soaked in nutrient solution, and the nutrient solution can replace soil and provide growth factors such as water, nutrients, oxygen and the like for the plants, so that the plants can normally grow. By adopting a water culture method, people can manually prepare a solution with balanced nutrition to realize balanced diet of plants. On the other hand, a scientific researcher can treat a material by adding a specific substance to a culture solution according to the purpose of an experiment, and the specific treatment of the material by using a hydroponic planting method is simpler and more uniform than that of a soil-planted material. In addition, scientific researchers can also analyze root metabolites of planted materials in nutrient solutions as needed, which is difficult to achieve in soil culture. Therefore, the water culture has the advantage that the soil culture cannot be replaced no matter the cultivation is carried out or the scientific research is carried out.
At present, the method for planting arabidopsis on nutrient soil and a culture medium is relatively mature, and the water culture method is still to be perfected. The current reported hydroponic methods are all based on indoor small-scale cultivation, and large-scale arabidopsis hydroponic cultivation has certain difficulty at present, mainly because of high cost of hydroponic cultivation and low survival rate of grown seedlings compared with soil cultivation. There are two main methods of hydroponics previously reported: the first is a seedling transplanting method, in which Arabidopsis thaliana grown on a culture medium for 1-3 weeks is transplanted into a hydroponic device. The second method is a comprehensive method, namely seeds directly germinate and grow on a water culture device, and the method needs to inject a culture medium with an appropriate concentration into an eppendorf tube with a cut bottom, but the germination rate of the seeds in the culture medium is poorer than that of the seeds in a sterile culture medium, and the seeds can fall off when the concentration of the culture medium is not appropriate, so that the subsequent steps cannot be carried out. The device for fixing the arabidopsis thaliana and the device for containing the nutrient solution used in the two methods are the key points in the water culture method. Among the previously reported fixtures are perforated lunch box covers, perforated polyethylene sheets, centrifuge tube racks, etc. The devices for containing the nutrient solution comprise lunch boxes, plastic basins and the like which are wrapped by tinfoil paper, and professional tools and devices are required for manufacturing the devices, so that the manufacturing process of the devices is complex, time-consuming and cost-consuming.
Disclosure of Invention
In view of the above problems, the present invention is to provide an arabidopsis thaliana hydroponic device with simple operation and low cost.
In order to achieve the above object, the utility model adopts the following technical scheme, an arabidopsis thaliana hydroponic device, a serial communication port, include:
the cultivation box adopts an opaque box body, the top of the cultivation box is open, and nutrient solution is placed inside the cultivation box;
the cultivation plate comprises a cultivation plate body horizontally arranged in the cultivation box and a plurality of cultivation cup bodies which are protruded and uniformly distributed at the bottom of the cultivation plate body, and the bottoms of the cultivation cup bodies are open;
the shading plate horizontally covers the upper portion of the cultivation plate, and a plurality of through holes communicated with the cultivation cup bodies in a one-to-one correspondence mode are formed in the shading plate.
Preferably, the cultivation plate body horizontally covers the top opening of the cultivation box, support columns are respectively inserted into the cultivation cup bodies at four corners of the top of the cultivation plate body, the preservative film covers the cultivation box through the four support columns, and the peripheral edge of the preservative film is fixed to the edge of the opening of the cultivation box.
Preferably, the bottom of the cultivation cup body positioned at the four corners of the top of the cultivation plate body is in a closed state.
Preferably, the open top periphery of the cultivation box is formed with an outward extending table.
Preferably, the cultivation box is in an inverted trapezoidal structure with a large top and a small bottom.
Preferably, the cultivation box is a colored plastic box.
Preferably, the culture plate is a PCR plate.
Preferably, the light screen adopts the tin foil paper, the tin foil paper parcel is in on the top surface of the cultivation plate body of cultivation board, be formed with on the tin foil paper with a plurality of through-holes of cultivation cup one-to-one.
Preferably, the support column adopts a 200 μ l gun head.
The utility model discloses owing to take above technical scheme, it has following advantage:
1. the utility model discloses a hydroponic device is including cultivation box, cultivation board and light screen, and the bottom protrusion equipartition of cultivation board has a plurality of cultivation cups, and the bottom of cultivation cup is uncovered, shifts the arabidopsis thaliana seedling to in the cultivation cup with tweezers, and the nutrient solution in the cultivation box provides the required nutrient substance of seedling, whole horizontal installation simple structure, and convenient operation need not complicated preparation in earlier stage, save time and cost.
2. The utility model discloses a cultivation board can adopt the PCR board, and the interval is unanimous between the hole of PCR board, can adjust the distance between the plant at will as required (like two holes of plant interval, three holes or four holes), overcomes among the prior art plant and places the rigidity in hole, wants to increase or reduce the interval must all the drawback of driling again.
3. The utility model discloses a cultivation box adopts coloured plastic box, and the cultivation board adopts the PCR board, and the light screen adopts the tin foil paper, and the support column adopts the rifle head, and each component source in the whole device is extensive, reduces the cost of whole device, cuts the hole on the PCR board according to plant interval demand during the use to correspond on the tin foil paper and cut the hole, utilize the rifle head to insert in PCR board four corners as the support column, whole device easy operation is easily promoted.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic top view of the structure of FIG. 1;
fig. 3 is another schematic structural diagram of the present invention.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings and examples.
As shown in fig. 1 and 2, the utility model provides an arabidopsis thaliana hydroponic device, which comprises a cultivation box 1, wherein an opaque box body is adopted, the top of the box body is open, and nutrient solution is placed inside the box body; the cultivation plate 2 comprises a cultivation plate body 21 horizontally covering the open top of the cultivation box 1 and a plurality of cultivation cup bodies 22 protruding and uniformly distributed at the bottom of the cultivation plate body 21, wherein the bottoms of the cultivation cup bodies 22 are open; and a shading plate 3 horizontally covering the upper part of the cultivation plate 2, wherein a plurality of through holes 31 which are communicated with the cultivation cup bodies 22 in a one-to-one correspondence manner are formed on the shading plate 3.
In the above embodiment, preferably, a support column 4 is respectively inserted into the cultivation cup 22 at the four corners of the top of the cultivation plate body 21, the preservative film 5 is covered above the cultivation box 1 through the four support columns 4, and the peripheral edge of the preservative film 5 is fixed on the open edge of the cultivation box 1, so that a heat-preserving and moisture-preserving growth condition can be created for arabidopsis thaliana just transferred into the hydroponics device.
In the above embodiment, it is preferable that the bottom of the cultivation cup 22 located at the four corners of the top of the cultivation plate body 21 is closed in order to stably insert the support column 4 into the cultivation cup 22.
In the above embodiment, it is preferable that the outer extension table 11 is formed on the outer circumference of the open top of the cultivation box 1 as shown in fig. 3, so that the cultivation plate 2 can be stably placed at the open top of the cultivation box 1.
In the above embodiment, it is preferable that the cultivating box 1 has an inverted trapezoidal structure with a large top and a small bottom.
In the above embodiment, preferably, the cultivation box 1 may adopt a colored plastic box, such as a blue or black plastic box, which has a good light shading effect;
in the above embodiment, it is preferable that the cultivation plate 2 is a PCR plate, such as a 96-well PCR plate, and the bottom of the PCR plate can be cut at a suitable position by scissors according to the plant spacing requirement, and an opening is formed at the bottom of the PCR plate to facilitate the transfer of Arabidopsis thaliana. According to the specification and size of the cultivation box 1, a plurality of PCR plates can be placed in the cultivation box 1.
In the above embodiment, preferably, the shading plate 3 is made of tinfoil paper, the tinfoil paper is wrapped on the top surface of the cultivation plate body 21 of the cultivation plate 2 to prevent the root greening caused by the later-stage light irradiation of the hydroponic liquid from affecting the growth of arabidopsis thaliana, a plurality of through holes corresponding to the plurality of cultivation cup bodies 22 one to one are formed in the tinfoil paper, and the tinfoil paper can be punched by a 200 μ l gun head.
In the above embodiment, preferably, the support column 4 can adopt 200 μ l tips, a 200 μ l tip is correspondingly inserted into four corners of the top of the PCR plate as a support, and the top is covered with the preservative film 5, so as to create a heat-preserving and moisture-preserving condition for the seedlings just moved to the water culture device.
The arabidopsis thaliana hydroponic device provided by the embodiment has the following specific hydroponic method:
1) the planting material is Arabidopsis thaliana ecotype Columbia, and the vernalized seeds at 4 deg.C are sterilized in 12% queen bleached water for 10-15min, and then washed with sterile water for 5 times. Seeded on 1/2MS medium and grown in light culture room (22 ℃, 16h light/8 h dark). And (5) moving to a water culture device for culture when the plant grows for 12 d.
2) The assembly of the hydroponic device is carried out,
3) pouring prepared 1/3MS nutrient solution into the cultivation box 1;
4) slightly taking out the arabidopsis seedlings growing for 12d on the culture medium and growing uniformly by using a small forceps, wherein the action needs to be gentle and slow so as to avoid injuring roots;
5) wrapping the root of Arabidopsis thaliana with a proper amount of cotton, and moving the cotton into a cultivation cup body 22 on a cultivation plate 2 by using a small forceps;
6) after all arabidopsis thaliana are transferred to a water culture device, a support column 4 is correspondingly inserted into four culture cup bodies 22 at four corners of the top of a culture plate body 21 respectively, and a preservative film 5 is covered on the support columns to make a heat preservation and moisture preservation condition for the seedlings which are just transferred to the water culture device.
7) According to the growth condition of the transplanted arabidopsis thaliana, the preservative film 5 is slowly uncovered after the arabidopsis thaliana is cultured for about 7-10 days in a lighting culture room, and the environmental humidity is kept to be about 50% after the preservative film 5 is uncovered. And replacing the hydroponic solution every 5 days, and growing for about 5 weeks to obtain mature arabidopsis thaliana plants.
The present invention has been described only with reference to the above embodiments, and the structure, arrangement position and connection of the components may be changed. On the basis of the technical scheme of the utility model, the all sides according to the utility model discloses the principle is all not excluded to the improvement that individual part goes on or the transform of equivalence the utility model discloses a protection scope is outside.
Claims (9)
1. An arabidopsis hydroponic device, comprising:
the cultivation box (1) adopts an opaque box body, the top of the cultivation box (1) is open, and nutrient solution is placed inside the cultivation box;
the cultivation plate (2) comprises a cultivation plate body (21) horizontally arranged in the cultivation box (1) and a plurality of cultivation cup bodies (22) protruding and uniformly distributed at the bottom of the cultivation plate body (21), and the bottoms of the cultivation cup bodies (22) are open;
the shading plate (3) horizontally covers the upper part of the cultivation plate (2), and a plurality of through holes (31) which are communicated with the cultivation cup bodies (22) in a one-to-one correspondence mode are formed in the shading plate (3).
2. The hydroponics device of arabidopsis thaliana of claim 1, wherein: the cultivation plate body (21) horizontally covers the top opening of the cultivation box (1), a support column (4) is respectively inserted into the cultivation cup bodies (22) at four corners of the top of the cultivation plate body (21), the preservative film (5) covers the cultivation box (1) through four support columns (4), and the edges of the periphery of the preservative film (5) are fixed to the edge of the opening of the cultivation box (1).
3. The hydroponics device of arabidopsis thaliana of claim 2, wherein: the bottom of the cultivation cup body (22) positioned at the four corners of the top of the cultivation plate body (21) is in a closed state.
4. The hydroponics device of arabidopsis thaliana of claim 1, wherein: an outer extension table (11) is formed on the periphery of the top opening of the cultivation box (1).
5. The hydroponics device of arabidopsis thaliana of claim 1, wherein: the cultivation box (1) is of an inverted trapezoidal structure with a large upper part and a small lower part.
6. The hydroponics device of arabidopsis thaliana of claim 1, wherein: the cultivation box (1) adopts a colored plastic box.
7. The hydroponics device of arabidopsis thaliana of claim 1, wherein: the culture plate (2) adopts a PCR plate.
8. The hydroponics device of arabidopsis thaliana of claim 1, wherein: the light screen (3) adopts the tin foil paper, the tin foil paper parcel is in on the top surface of the cultivation plate body (21) of cultivation board (2), be formed with on the tin foil paper with a plurality of through-holes of cultivation cup (22) one-to-one.
9. The hydroponics device of arabidopsis thaliana of claim 2, wherein: and the supporting column (4) adopts a 200-microliter gun head.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2019209900704 | 2019-06-28 | ||
| CN201920990070 | 2019-06-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211407170U true CN211407170U (en) | 2020-09-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201922131168.7U Active CN211407170U (en) | 2019-06-28 | 2019-12-03 | Water culture device for arabidopsis thaliana |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113057046A (en) * | 2021-04-06 | 2021-07-02 | 四维生态科技（杭州）有限公司 | Plant planting device and method |
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2019
- 2019-12-03 CN CN201922131168.7U patent/CN211407170U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113057046A (en) * | 2021-04-06 | 2021-07-02 | 四维生态科技（杭州）有限公司 | Plant planting device and method |
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