CN103808544B - A kind of easily extensible soil microbe fumigating device - Google Patents
A kind of easily extensible soil microbe fumigating device Download PDFInfo
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- CN103808544B CN103808544B CN201410056586.3A CN201410056586A CN103808544B CN 103808544 B CN103808544 B CN 103808544B CN 201410056586 A CN201410056586 A CN 201410056586A CN 103808544 B CN103808544 B CN 103808544B
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Abstract
The invention discloses a kind of easily extensible soil microbe fumigating device.Comprise air feed part, sample cup and exhaust portion; Gas supply part divides and comprises gas row group and reagent bottle; Gas row group is for leading to by several threeways and several right angles two circuit formed that is connected, and one end of circuit is connected with reagent bottle; Valve is provided with between reagent bottle and circuit; The upright opening of threeway is connected with air tube I by pipeline; Gas row is mounted on supporting plate; Exhaust portion comprises gas row group and vacuum pump; One end of gas row group is connected with vacuum pump; Valve is provided with between vacuum pump and gas row group; The upright opening of described threeway is connected with air tube II by pipeline.Soil microbe fumigating device of the present invention adopts initiatively fumigation technology, and namely fumigating gas enters from the bottom of sample cup, by being placed on the pedotheque on screen cloth, along with draft is discharged to the external world by exhaust portion gas piping; Increase the contact area of soil and chloroform compared with legacy equipment, add vapor flux, thus stifling efficiency can be improved and shorten fumigation time.
Description
Technical field
The present invention relates to a kind of easily extensible soil microbe fumigating device, be applicable to Soil Microorganism biomass C, nitrogen, phosphorus yield.
Background technology
Existing exsiccator, mostly be material glass, be placed with a ceramic wafer with holes in the middle part of base, can take out, above be stamped valve, for controlling the opening of container, during use, soil is loaded small beaker, put into exsiccator, vaseline airtight container is smeared at the exsiccator place of combining between the higher and lower levels, open upper valve connection vacuum pump to bleed, valve-off and vacuum pump after chloroform seethes with excitement 1 minute; The deficiency of existing equipment is: poor air-tightness, and glass material is fragile, and when bleeding, chloroform vapor is without soil, is just full of container, stifling not thorough.
Summary of the invention
The object of this invention is to provide a kind of easily extensible soil microbe fumigating device, the present invention can improve soil microorganism and fumigate efficiency, shortens the process extracted.
A kind of easily extensible soil microbe fumigating device provided by the present invention, comprises air feed part, sample cup and exhaust portion;
Described gas supply part divides and comprises gas row group and reagent bottle; Described gas row group is for leading to by several threeways and several right angles two circuit formed that is connected, and one end of described circuit is connected with described reagent bottle; Valve is provided with between described reagent bottle and described circuit; The upright opening of described threeway is connected with air tube I by a pipeline;
Described gas row is mounted on a supporting plate, and the top of described gas row group is provided with a base plate, and described base plate is provided with several through holes, and described through hole is corresponding with the position of described air tube I, and described air tube I is inserted in described through hole; Supported by a pole between described base plate and described supporting plate;
Described exhaust portion comprises described gas row group and vacuum pump; One end of described gas row group is connected with described vacuum pump; Valve is provided with between described vacuum pump and described gas row group; The upright opening of described threeway is connected with air tube II by a pipeline;
Described sample cup comprises cup, spiral cover I, spiral cover II, elasticity through hole post I, elasticity through hole post II, gland I and gland II; The both ends open of described cup, the lower end of described cup is tightly connected with described spiral cover I and described gland I successively, and is provided with a screen cloth between described cup and described spiral cover I, is provided with described elasticity through hole post I between described gland I and described spiral cover I; Described gland I is connected with the through hole of described elasticity through hole post I with the through hole that described spiral cover I is arranged, and described air tube I is connected with described cup with the through hole of described elasticity through hole post I by described gland I, described spiral cover I; The upper end of described cup is tightly connected with described spiral cover II and described gland II successively, and is provided with described elasticity through hole post II between described gland II and described spiral cover II; Described gland II is connected with the through hole of described elasticity through hole post II with the through hole that described spiral cover II is arranged, and described air tube II is connected with described cup with the through hole of described elasticity through hole post II by described gland II, described spiral cover II.
In microorganism device for fumigation of the present invention, the lateral aperture of described threeway is interconnected, described right angle two is logical to be connected with the lateral aperture of described threeway, microorganism device for fumigation of the present invention by described threeway and described right angle two logical realizes laterally with longitudinal expansion, the number that the threeway of setting and right angle two lead to can be regulated and controled according to actual needs.
In above-mentioned microorganism device for fumigation, supported by screw between described base plate and described supporting plate, to support described sample cup.
In above-mentioned microorganism device for fumigation, be provided with vacuum meter between described vacuum pump and described gas row group, for measuring the pressure in described sample cup.
In above-mentioned microorganism device for fumigation, described elasticity through hole post I and described elasticity through hole post II are through hole fluororubber post, described gland I and described spiral cover I and described gland II and described spiral cover II play airtight effect by screwing squeezing through-hole fluororubber post and making its center through hole shrink, and when air tube I inserts with air tube II, gas circuit is then communicated with.
In above-mentioned microorganism device for fumigation, between described spiral cover I and described cup and between described spiral cover II and described cup, be equipped with fluorine rubber ring, to strengthen the effect of sealing.
In above-mentioned microorganism device for fumigation, described mesh screen specifically can be stainless steel mesh screen.
In above-mentioned microorganism device for fumigation, all sealed by anchor ear between described pipeline and described air tube I and between described pipeline and described air tube II.
Easily extensible soil microbe fumigating device provided by the invention, adopt initiatively fumigation technology, namely fumigating gas enters from the bottom of sample cup, by being placed on the pedotheque on screen cloth, along with draft is discharged to the external world by exhaust portion gas piping; Compare with classic method the contact area increasing soil and chloroform with equipment, add vapor flux, thus stifling efficiency can be improved and shorten fumigation time.
Accompanying drawing explanation
Fig. 1 is the structural representation of easily extensible soil microbe fumigating device of the present invention.
Fig. 2 is that in easily extensible soil microbe fumigating device of the present invention, gas circuit connects (air feed part and exhaust portion) schematic diagram.
Fig. 3 is the decomposition texture schematic diagram of sample cup in easily extensible soil microbe fumigating device of the present invention.
In figure, each mark is as follows:
1 air feed part, 2 sample cups, 3 exhaust portion, 4 supporting plates, 5 base plates, 6 screws, 7 gas circuit row groups, 8a, 8b valve, 9 reagent bottles, 10 vacuum meters, 11 vacuum pumps, 12 air tubes I (air tube II), 13 anchor ears, 14 pipelines, 15 threeways, 16 right angle two logical, 171 gland I, 172 gland II, 181 through hole fluororubber post I, 182 through hole fluororubber post II, 191 spiral cover I, 192 spiral cover II, 201 fluorine rubber ring I, 202 fluorine rubber ring II, 21 cups, 22 screen clothes.
Embodiment
Below in conjunction with accompanying drawing, the present invention will be further described, but the present invention is not limited to following examples.
As shown in Figure 1, easily extensible soil microbe fumigating device provided by the invention comprises air feed part 1, sample cup 2 and exhaust portion 3.As shown in Figure 2, air feed part 1 comprises gas row and organizes 7 and reagent bottle 9, and wherein gas row organizes 7 for the circuit formed that is connected by multiple threeway 15 and multiple right angle two logical 16, and one end of this circuit is connected with reagent bottle 9.Valve 8a is provided with between this reagent bottle 9 and circuit; The upright opening of threeway 15 is connected with air tube I by pipeline 14, and is sealed by anchor ear 13.As shown in Figure 1, gas row organizes 7 and is located on supporting plate 4, and the top that gas row organizes 7 is provided with base plate 5, and this base plate 5 is provided with multiple through hole (not marking in figure), and these through holes are corresponding with the position of air tube I 12, and air tube I 12 can be inserted in this through hole.Supported by screw 6 between base plate 5 and supporting plate 4.
As depicted in figs. 1 and 2, exhaust portion 3 comprises that gas row organizes 7, vacuum meter 10 and vacuum pump 11, and one end that this gas row organizes 7 is connected with vacuum pump 11, the pipeline of this connection is arranged valve 8b and vacuum meter 10.The upright opening of threeway 15 is connected with air tube II 12 by pipeline 14.
As shown in figures 1 and 3, sample cup 2 comprises cup 21, spiral cover I 191, spiral cover II 192, through hole fluororubber post I 181, through hole fluororubber post 182 II, gland I 171 and gland II 172.The wherein both ends open of cup 21, the lower end of cup 21 is tightly connected with spiral cover I 191 and gland I 171 successively, is specially and is threaded, be provided with fluorine rubber ring I between spiral cover I 191 and cup 21, to strengthen the effect of sealing.A screen cloth 22 is provided with, for holding pedotheque between cup 21 and spiral cover I 191.And between gland I 171 and spiral cover I 191, be provided with through hole fluororubber post I 181, gland I 171 is connected with the through hole of through hole fluororubber post I 181 with the through hole that spiral cover I 191 is arranged, thus air tube I 12 is connected with cup 21 with the through hole of through hole fluororubber post I 181 by gland I 171, spiral cover I 191.The upper end of cup 21 is tightly connected with spiral cover II 192 and gland II 172 successively, is specially and is threaded, be provided with fluorine rubber ring II between spiral cover II 192 and cup 21, to strengthen the effect of sealing.And between gland II 172 and spiral cover II 192, be provided with through hole fluororubber post II 182, gland II 172 is connected with the through hole of through hole fluororubber post II 182 with the through hole that spiral cover II 192 is arranged, and air tube II 12 is connected with cup 21 with the through hole of through hole fluororubber post II 182 by gland II 172, spiral cover II 192.
Microorganism device for fumigation provided by the invention, in gas row group, the lateral aperture of threeway is interconnected, right angle two is logical to be connected with the lateral aperture of threeway, microorganism device for fumigation of the present invention by threeway and right angle two logical realizes laterally with longitudinal expansion, the number that the threeway of setting and right angle two lead to can be regulated and controled according to actual needs.
Microorganism device for fumigation provided by the invention, the material of cup 21 also may be selected to be the material of teflon or other resistance to chloroform corrosion; Also coordinate by alternate manners such as interference fit between gland, spiral cover, cup; The screen cloth 22 order number arranged in this device can adjust according to actual needs.
When using above-mentioned easily extensible soil microbe fumigating device, first air feed part 1 and exhaust portion 2 is assembled, and then ethanol chloroform is added in reagent bottle 9, then the Lower Half of sample cup 2 is first assembled, pedotheque is evenly placed on screen cloth 22, the first half of sealed sample cup again, screw that sample cup spiral cover 191 is airtight to be steadily pressed onto on base plate 5 afterwards, make air tube I 12 corresponding with sample cup bottom opening, be communicated with gas circuit, after installing sample cup, the row of bleeding is organized air tube II 12 correspondence insertion sample cup top tapping, thus through gas circuit; Next step gas circuit row opening air feed part 1 and exhaust portion 3 organizes valve 8a and 8b, connect vacuum pump 11 power supply to bleed, after chloroform boiling, steam is by the sample on screen cloth 22, first valve-off 8b and vacuum pump 11 after chloroform seethes with excitement 1 minute, after a while valve-off 8a again, extract the row of bleeding and organize air tube II 12, upwards extract sample cup 2, under sample cup 2 is placed in 25 degrees Celsius, carry out 24 hours suffocating treatment; After terminating, sample cup 2 is pressed on base plate again, and air tube II 12 of bleeding inserts sample cup upper hole position, now need to remove reagent bottle 9 and close gas supply part sub valve 8a, only opening valve 8b and connecting vacuum pump 11 power supply carries out except chloroform operation, take out and close 8b in 2-3 minute, open 8a air inlet, after balance, close 8a again, open 8b and repeatedly bleed until chloroform in soil is taken out to the greatest extent.
Claims (7)
1. an easily extensible soil microbe fumigating device, is characterized in that:
Described device for fumigation comprises air feed part, sample cup and exhaust portion;
Described gas supply part divides and comprises gas row group and reagent bottle; Described gas row group is for leading to by several threeways and several right angles two circuit formed that is connected, and one end of described circuit is connected with described reagent bottle; Valve is provided with between described reagent bottle and described circuit; The upright opening of described threeway is connected with air tube I by a pipeline;
Described gas row is mounted on a supporting plate, and the top of described gas row group is provided with a base plate, and described base plate is provided with several through holes, and described through hole is corresponding with the position of described air tube I, and described air tube I is inserted in described through hole; Supported by a pole between described base plate and described supporting plate;
Described exhaust portion comprises described gas row group and vacuum pump; One end of described gas row group is connected with described vacuum pump; Valve is provided with between described vacuum pump and described gas row group; The upright opening of described threeway is connected with air tube II by a pipeline;
Described sample cup comprises cup, spiral cover I, spiral cover II, elasticity through hole post I, elasticity through hole post II, gland I and gland II; The both ends open of described cup, the lower end of described cup is tightly connected with described spiral cover I and described gland I successively, and is provided with a screen cloth between described cup and described spiral cover I, is provided with described elasticity through hole post I between described gland I and described spiral cover I; Described gland I is connected with the through hole of described elasticity through hole post I with the through hole that described spiral cover I is arranged, and described air tube I is connected with described cup with the through hole of described elasticity through hole post I by described gland I, described spiral cover I; The upper end of described cup is tightly connected with described spiral cover II and described gland II successively, and is provided with described elasticity through hole post II between described gland II and described spiral cover II; Described gland II is connected with the through hole of described elasticity through hole post II with the through hole that described spiral cover II is arranged, and described air tube II is connected with described cup with the through hole of described elasticity through hole post II by described gland II, described spiral cover II.
2. microorganism device for fumigation according to claim 1, be is characterized in that: supported by screw between described base plate and described supporting plate.
3. microorganism device for fumigation according to claim 1 and 2, is characterized in that: be provided with vacuum meter between described vacuum pump and described gas row group.
4. microorganism device for fumigation according to claim 1, is characterized in that: described elasticity through hole post I and described elasticity through hole post II are through hole fluororubber post.
5. microorganism device for fumigation according to claim 1, is characterized in that: be equipped with fluorine rubber ring between described spiral cover I and described cup and between described spiral cover II and described cup.
6. microorganism device for fumigation according to claim 1, is characterized in that: described screen cloth is stainless steel mesh.
7. microorganism device for fumigation according to claim 1, be is characterized in that: all sealed by anchor ear between described pipeline and described air tube I and between described pipeline and described air tube II.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410056586.3A CN103808544B (en) | 2014-02-19 | 2014-02-19 | A kind of easily extensible soil microbe fumigating device |
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| CN201410056586.3A CN103808544B (en) | 2014-02-19 | 2014-02-19 | A kind of easily extensible soil microbe fumigating device |
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| CN103808544A CN103808544A (en) | 2014-05-21 |
| CN103808544B true CN103808544B (en) | 2016-03-16 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104764639B (en) * | 2015-03-19 | 2017-11-14 | 新疆农业科学院土壤肥料与农业节水研究所 | A kind of soil microbe fumigating device and its application |
| CN105277404B (en) * | 2015-10-08 | 2018-01-23 | 浙江省农业科学院 | Determine soil microbes biomass carbon nitrogen chloroform device for fumigation and fumigating method |
| CN106404514A (en) * | 2015-11-29 | 2017-02-15 | 杭州跟策科技有限公司 | Chloroform fumigating apparatus |
| CN105638626B (en) * | 2015-12-24 | 2018-05-04 | 李晓亮 | Soil-fumigating capsule, stifling leaching device and stifling extracting technology |
| CN111751185A (en) * | 2020-07-07 | 2020-10-09 | 盐城工学院 | Soil microorganism fumigation device |
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| JP2000234990A (en) * | 1999-02-17 | 2000-08-29 | Dainippon Ink & Chem Inc | Device for analyzing trace volatile gas constituent |
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