CN103808544A - Expandable soil microorganism fumigating device - Google Patents
Expandable soil microorganism fumigating device Download PDFInfo
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- CN103808544A CN103808544A CN201410056586.3A CN201410056586A CN103808544A CN 103808544 A CN103808544 A CN 103808544A CN 201410056586 A CN201410056586 A CN 201410056586A CN 103808544 A CN103808544 A CN 103808544A
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Abstract
The invention discloses an expandable soil microorganism fumigating device which comprises a gas supply part, a sample cup and a gas extraction part, wherein the gas supply part comprises a gas row group and a reagent bottle; the gas row group is a line formed by connecting a plurality of three-way joints with a plurality of right-angle two-way joints; one end of each line is communicated with the reagent bottle; a valve is arranged between the reagent bottle and the line; perpendicular holes of the three-way joints are communicated with gas needles I through pipelines; the gas row group is arranged on a bracket plate; the gas extraction part comprises a gas row group and a vacuum pump; one end of the gas row group is communicated with the vacuum pump; a valve is arranged between the vacuum pump and the gas row group; the perpendicular holes of the three-way joints are communicated with gas needles II through pipelines. The expandable soil microorganism fumigating device disclosed by the invention adopts an active fumigating technology, namely fumigated gas enters the sample cup from the bottom of the sample cup, passes through a soil sample arranged on a screen and is discharged to the outside through a gas pipeline of the gas extraction part along with gas extraction. Compared with the conventional equipment, the expandable soil microorganism fumigating device has the advantages that the contact area of soil and chloroform is enlarged, and the steam flux is increased, and therefore, the fumigating efficiency can be improved, and the fumigating time can be shortened.
Description
Technical field
The present invention relates to one and can expand soil microbe fumigating device, be applicable to the mensuration of Soil Microorganism biomass C, nitrogen, phosphorus.
Background technology
Existing exsiccator, mostly be material glass, base middle part is placed with a ceramic wafer with holes, can take out, and is above stamped valve, for controlling the opening of container, when use, pack soil into small beaker, put into exsiccator, vaseline airtight container is smeared at the exsiccator place of combining between the higher and lower levels, open upper valve connection vacuum pump and bleed, valve-off and vacuum pump after chloroform seethes with excitement 1 minute; The deficiency of existing equipment is: poor air-tightness, and glass material is fragile, and while bleeding, chloroform vapor, without soil, is just full of container, stifling not thorough.
Summary of the invention
The object of this invention is to provide one and can expand soil microbe fumigating device, the present invention can improve the stifling efficiency of soil microorganism, shortens the process of extracting.
One provided by the present invention can be expanded soil microbe fumigating device, comprises air feed part, sample cup and the part of bleeding;
Described gas supply part divides and comprises gas row group and reagent bottle; The described gas row group logical circuit that is connected and forms of several threeways and several right angles two of serving as reasons, one end of described circuit is connected with described reagent bottle; Between described reagent bottle and described circuit, be provided with valve; The upright opening of described threeway is connected with air tube I by a pipeline;
Described gas row is mounted on a supporting plate, and the top of described gas row group is provided with a base plate, and described base plate is provided with several through holes, and described through hole is corresponding with the position of described air tube I, and described air tube I is inserted in described through hole; Between described base plate and described supporting plate, support by a pole;
The described part of bleeding comprises described gas row group and vacuum pump; One end of described gas row group is connected with described vacuum pump; Between described vacuum pump and described gas row group, be provided with valve; The upright opening of described threeway is connected with air tube II by a pipeline;
Described sample cup comprises cup, spiral cover I, spiral cover II, elasticity through hole post I, elasticity through hole post II, gland I and gland II; The both ends open of described cup, the lower end of described cup is tightly connected with described spiral cover I and described gland I successively, and is provided with a screen cloth between described cup and described spiral cover I, is provided with described elasticity through hole post I between described gland I and described spiral cover I; The through hole arranging in described gland I and described spiral cover I is connected with the through hole of described elasticity through hole post I, and described air tube I is connected with described cup by the through hole of described gland I, described spiral cover I and described elasticity through hole post I; The upper end of described cup is tightly connected with described spiral cover II and described gland II successively, and between described gland II and described spiral cover II, is provided with described elasticity through hole post II; The through hole arranging in described gland II and described spiral cover II is connected with the through hole of described elasticity through hole post II, and described air tube II is connected with described cup by the through hole of described gland II, described spiral cover II and described elasticity through hole post II.
In microorganism device for fumigation of the present invention, the lateral aperture of described threeway is interconnected, described right angle two is logical to be connected with the lateral aperture of described threeway, microorganism device for fumigation of the present invention, by the horizontal and longitudinal expansion of the logical realization of described threeway and described right angle two, can regulate and control the logical number of threeway and right angle two of setting according to actual needs.
In above-mentioned microorganism device for fumigation, between described base plate and described supporting plate, support by screw, to support described sample cup.
In above-mentioned microorganism device for fumigation, between described vacuum pump and described gas row group, be provided with vacuum meter, for measuring the pressure in described sample cup.
In above-mentioned microorganism device for fumigation, described elasticity through hole post I and described elasticity through hole post II are through hole fluororubber post, described gland I and described spiral cover I and described gland II and described spiral cover II make its center through hole shrink the airtight effect of playing by screwing squeezing through-hole fluororubber post, and in the time of air tube I and the insertion of air tube II, gas circuit is communicated with.
In above-mentioned microorganism device for fumigation, between described spiral cover I and described cup and between described spiral cover II and described cup, be equipped with fluorine rubber ring, to strengthen the effect of sealing.
In above-mentioned microorganism device for fumigation, described mesh screen specifically can be stainless steel mesh screen.
In above-mentioned microorganism device for fumigation, between described pipeline and described air tube I and between described pipeline and described air tube II, all seal by anchor ear.
The soil microbe fumigating device of expanding provided by the invention, adopts initiatively fumigation technology, and fumigating gas enters from the bottom of sample cup, by being placed on the pedotheque on screen cloth, along with draft is discharged to the external world by the portion gas pipeline of bleeding; Compare the contact area that has increased soil and chloroform with classic method with equipment, increased steam flux, thereby can improve stifling efficiency and shorten fumigation time.
Accompanying drawing explanation
Fig. 1 is the structural representation that the present invention can expand soil microbe fumigating device.
Fig. 2 is that the present invention can expand gas circuit connection in soil microbe fumigating device (air feed part and the part of bleeding) schematic diagram.
Fig. 3 is the decomposition texture schematic diagram that the present invention can expand sample cup in soil microbe fumigating device.
In figure, each mark is as follows:
1 air feed part, 2 sample cups, 3 bleed part, 4 supporting plates, 5 base plates, 6 screws, 7 gas circuit row groups, 8a, 8b valve, 9 reagent bottles, 10 vacuum meters, 11 vacuum pumps, 12 air tube I (air tube II), 13 anchor ears, 14 pipelines, 15 threeways, 16 right angles two are logical, 171 gland I, 172 gland II, 181 through hole fluororubber post I, 182 through hole fluororubber post II, 191 spiral cover I, 192 spiral cover II, 201 fluorine rubber ring I, 202 fluorine rubber ring II, 21 cups, 22 screen clothes.
Embodiment
Below in conjunction with accompanying drawing, the present invention will be further described, but the present invention is not limited to following examples.
As shown in Figure 1, the soil microbe fumigating device of expanding provided by the invention comprises air feed part 1, sample cup 2 and the part 3 of bleeding.As shown in Figure 2, air feed part 1 comprises that gas row organizes 7 and reagent bottle 9, and wherein gas row organizes 7 for the circuit forming that is connected by multiple threeways 15 and multiple right angles two logical 16, and one end of this circuit is connected with reagent bottle 9.Between this reagent bottle 9 and circuit, be provided with valve 8a; The upright opening of threeway 15 is connected with air tube I by pipeline 14, and seals by anchor ear 13.As shown in Figure 1, gas row organizes 7 and is located on supporting plate 4, and gas row organizes 7 top and is provided with base plate 5, and this base plate 5 is provided with multiple through holes (in figure not mark), and these through holes are corresponding with the position of air tube I 12, and air tube I 12 can be inserted in this through hole.Between base plate 5 and supporting plate 4, support by screw 6.
As depicted in figs. 1 and 2, the part of bleeding 3 comprises that gas row organizes 7, vacuum meter 10 and vacuum pump 11, and this gas row organizes one end of 7 and is connected with vacuum pump 11, and valve 8b and vacuum meter 10 are set on the pipeline of this connection.The upright opening of threeway 15 is connected with air tube II 12 by pipeline 14.
As shown in figures 1 and 3, sample cup 2 comprises cup 21, spiral cover I 191, spiral cover II 192, through hole fluororubber post I 181, through hole fluororubber post 182 II, gland I 171 and gland II 172.The wherein both ends open of cup 21, the lower end of cup 21 is tightly connected with spiral cover I 191 and gland I 171 successively, is specially and is threaded, and between spiral cover I 191 and cup 21, is provided with fluorine rubber ring I, to strengthen the effect of sealing.Between cup 21 and spiral cover I 191, be provided with a screen cloth 22, for holding pedotheque.And between gland I 171 and spiral cover I 191, be provided with through hole fluororubber post I 181, the through hole arranging in gland I 171 and spiral cover I 191 is connected with the through hole of through hole fluororubber post I 181, thereby air tube I 12 is connected with cup 21 by the through hole of gland I 171, spiral cover I 191 and through hole fluororubber post I 181.The upper end of cup 21 is tightly connected with spiral cover II 192 and gland II 172 successively, is specially and is threaded, and between spiral cover II 192 and cup 21, is provided with fluorine rubber ring II, to strengthen the effect of sealing.And between gland II 172 and spiral cover II 192, be provided with through hole fluororubber post II 182, the through hole arranging in gland II 172 and spiral cover II 192 is connected with the through hole of through hole fluororubber post II 182, and air tube II 12 is connected with cup 21 by the through hole of gland II 172, spiral cover II 192 and through hole fluororubber post II 182.
Microorganism device for fumigation provided by the invention, in gas row group, the lateral aperture of threeway is interconnected, right angle two is logical to be connected with the lateral aperture of threeway, microorganism device for fumigation of the present invention, by the horizontal and longitudinal expansion of the logical realization of threeway and right angle two, can regulate and control the logical number of threeway and right angle two of setting according to actual needs.
Microorganism device for fumigation provided by the invention, the material of cup 21 also may be selected to be the material of teflon or other resistance to chloroform corrosion; Between gland, spiral cover, cup, also can coordinate by alternate manners such as interference fit; The screen cloth 22 order numbers that arrange in this device can be adjusted according to actual needs.
Use above-mentioned while expanding soil microbe fumigating device, first assemble air feed part 1 and the part 2 of bleeding, and then in reagent bottle 9, add ethanol chloroform, the Lower Half of then first assembling sample cup 2, pedotheque is evenly placed on screen cloth 22, the first half of sealed sample cup again, screw airtight rear being steadily pressed onto on base plate 5 of sample cup spiral cover 191, make air tube I 12 corresponding with sample cup bottom opening, be communicated with gas circuit, after installing sample cup, the row of bleeding is organized to the corresponding sample cup top tapping that inserts of air tube II 12, thereby connect gas circuit; Next step gas circuit row who opens air feed part 1 and the part 3 of bleeding organizes valve 8a and 8b, connecting vacuum pump 11 power supplys bleeds, after chloroform boiling, steam is by the sample on screen cloth 22, until chloroform boiling first valve-off 8b and vacuum pump 11 after 1 minute, valve-off 8a more after a while, extracts the row of bleeding and organizes air tube II 12, upwards extract sample cup 2, sample cup 2 is placed in and under 25 degrees Celsius, carries out 24 hours stifling processing; After finishing, sample cup 2 is pressed on base plate again, and the air tube II 12 of bleeding is inserted sample cup upper hole position, now need to remove reagent bottle 9 and close gas supply part sub valve 8a, only open valve 8b and connect vacuum pump 11 power supplys and remove chloroform operation, take out and within 2-3 minute, close 8b, open 8a air inlet, after balance, close again 8a, open that 8b bleeds repeatedly until chloroform in soil is taken out to the greatest extent.
Claims (7)
1. can expand a soil microbe fumigating device, it is characterized in that:
Described device for fumigation comprises air feed part, sample cup and the part of bleeding;
Described gas supply part divides and comprises gas row group and reagent bottle; The described gas row group logical circuit that is connected and forms of several threeways and several right angles two of serving as reasons, one end of described circuit is connected with described reagent bottle; Between described reagent bottle and described circuit, be provided with valve; The upright opening of described threeway is connected with air tube I by a pipeline;
Described gas row is mounted on a supporting plate, and the top of described gas row group is provided with a base plate, and described base plate is provided with several through holes, and described through hole is corresponding with the position of described air tube I, and described air tube I is inserted in described through hole; Between described base plate and described supporting plate, support by a pole;
The described part of bleeding comprises described gas row group and vacuum pump; One end of described gas row group is connected with described vacuum pump; Between described vacuum pump and described gas row group, be provided with valve; The upright opening of described threeway is connected with air tube II by a pipeline;
Described sample cup comprises cup, spiral cover I, spiral cover II, elasticity through hole post I, elasticity through hole post II, gland I and gland II; The both ends open of described cup, the lower end of described cup is tightly connected with described spiral cover I and described gland I successively, and is provided with a screen cloth between described cup and described spiral cover I, is provided with described elasticity through hole post I between described gland I and described spiral cover I; The through hole arranging in described gland I and described spiral cover I is connected with the through hole of described elasticity through hole post I, and described air tube I is connected with described cup by the through hole of described gland I, described spiral cover I and described elasticity through hole post I; The upper end of described cup is tightly connected with described spiral cover II and described gland II successively, and between described gland II and described spiral cover II, is provided with described elasticity through hole post II; The through hole arranging in described gland II and described spiral cover II is connected with the through hole of described elasticity through hole post II, and described air tube II is connected with described cup by the through hole of described gland II, described spiral cover II and described elasticity through hole post II.
2. microorganism device for fumigation according to claim 1, is characterized in that: between described base plate and described supporting plate, support by screw.
3. microorganism device for fumigation according to claim 1 and 2, is characterized in that: between described vacuum pump and described gas row group, be provided with vacuum meter.
4. according to the microorganism device for fumigation described in any one in claim 1-3, it is characterized in that: described elasticity through hole post I and described elasticity through hole post II are through hole fluororubber post.
5. according to the microorganism device for fumigation described in any one in claim 1-4, it is characterized in that: between described spiral cover I and described cup and between described spiral cover II and described cup, be equipped with fluorine rubber ring.
6. according to the microorganism device for fumigation described in any one in claim 1-5, it is characterized in that: described mesh screen is stainless steel mesh screen.
7. according to the microorganism device for fumigation described in any one in claim 1-6, it is characterized in that: between described pipeline and described air tube I and between described pipeline and described air tube II, all seal by anchor ear.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410056586.3A CN103808544B (en) | 2014-02-19 | 2014-02-19 | A kind of easily extensible soil microbe fumigating device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410056586.3A CN103808544B (en) | 2014-02-19 | 2014-02-19 | A kind of easily extensible soil microbe fumigating device |
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| CN103808544A true CN103808544A (en) | 2014-05-21 |
| CN103808544B CN103808544B (en) | 2016-03-16 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105277404A (en) * | 2015-10-08 | 2016-01-27 | 浙江省农业科学院 | Chloroform fumigation device for measuring soil microbial biomass carbon nitrogen and fumigation method |
| CN105638626A (en) * | 2015-12-24 | 2016-06-08 | 李晓亮 | Soil fumigation capsule, fumigation and digestion device and fumigation and digestion process |
| CN106404514A (en) * | 2015-11-29 | 2017-02-15 | 杭州跟策科技有限公司 | Chloroform fumigating apparatus |
| CN104764639B (en) * | 2015-03-19 | 2017-11-14 | 新疆农业科学院土壤肥料与农业节水研究所 | A kind of soil microbe fumigating device and its application |
| CN111751185A (en) * | 2020-07-07 | 2020-10-09 | 盐城工学院 | Soil microorganism fumigation device |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000234990A (en) * | 1999-02-17 | 2000-08-29 | Dainippon Ink & Chem Inc | Device for analyzing trace volatile gas constituent |
| CN2508249Y (en) * | 2001-09-18 | 2002-08-28 | 宋刚 | Gas fumigating device for thin-layer chromatography test |
| CN1466997A (en) * | 2002-12-30 | 2004-01-14 | 中华人民共和国北京出入境检验检疫局 | Method and equipment for disinfecting with vacuum circulation suffocating |
| CN101210882A (en) * | 2006-12-27 | 2008-07-02 | 中国科学院沈阳应用生态研究所 | Method for determining soil microbes biomass carbon |
| CN101526442A (en) * | 2007-08-20 | 2009-09-09 | 香港科技大学 | A high suction double-cell extractor |
| CN201740685U (en) * | 2010-07-22 | 2011-02-09 | 中国石油天然气股份有限公司 | Inert gas extraction and separation sample preparation system |
| CN102507286A (en) * | 2011-11-14 | 2012-06-20 | 中国科学院植物研究所 | Soil microbe fumigating device |
| CN202512006U (en) * | 2012-03-31 | 2012-10-31 | 中国地质科学院岩溶地质研究所 | Sample preparation device for carbon isotope of organic carbon |
-
2014
- 2014-02-19 CN CN201410056586.3A patent/CN103808544B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000234990A (en) * | 1999-02-17 | 2000-08-29 | Dainippon Ink & Chem Inc | Device for analyzing trace volatile gas constituent |
| CN2508249Y (en) * | 2001-09-18 | 2002-08-28 | 宋刚 | Gas fumigating device for thin-layer chromatography test |
| CN1466997A (en) * | 2002-12-30 | 2004-01-14 | 中华人民共和国北京出入境检验检疫局 | Method and equipment for disinfecting with vacuum circulation suffocating |
| CN101210882A (en) * | 2006-12-27 | 2008-07-02 | 中国科学院沈阳应用生态研究所 | Method for determining soil microbes biomass carbon |
| CN101526442A (en) * | 2007-08-20 | 2009-09-09 | 香港科技大学 | A high suction double-cell extractor |
| CN201740685U (en) * | 2010-07-22 | 2011-02-09 | 中国石油天然气股份有限公司 | Inert gas extraction and separation sample preparation system |
| CN102507286A (en) * | 2011-11-14 | 2012-06-20 | 中国科学院植物研究所 | Soil microbe fumigating device |
| CN202512006U (en) * | 2012-03-31 | 2012-10-31 | 中国地质科学院岩溶地质研究所 | Sample preparation device for carbon isotope of organic carbon |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104764639B (en) * | 2015-03-19 | 2017-11-14 | 新疆农业科学院土壤肥料与农业节水研究所 | A kind of soil microbe fumigating device and its application |
| CN105277404A (en) * | 2015-10-08 | 2016-01-27 | 浙江省农业科学院 | Chloroform fumigation device for measuring soil microbial biomass carbon nitrogen and fumigation method |
| CN106404514A (en) * | 2015-11-29 | 2017-02-15 | 杭州跟策科技有限公司 | Chloroform fumigating apparatus |
| CN105638626A (en) * | 2015-12-24 | 2016-06-08 | 李晓亮 | Soil fumigation capsule, fumigation and digestion device and fumigation and digestion process |
| CN105638626B (en) * | 2015-12-24 | 2018-05-04 | 李晓亮 | Soil-fumigating capsule, stifling leaching device and stifling extracting technology |
| CN111751185A (en) * | 2020-07-07 | 2020-10-09 | 盐城工学院 | Soil microorganism fumigation device |
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| CN103808544B (en) | 2016-03-16 |
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