TWI417381B - Cactus camellia fruiting body and mycelia fast culture flask device - Google Patents
Cactus camellia fruiting body and mycelia fast culture flask device Download PDFInfo
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Description
本發明係有關於一種「牛樟子實體及菌絲體快速培養瓶裝置」,尤指其利用一菌絲體培養瓶於瓶內裝填牛樟木屑及植入牛樟菌絲體,並於瓶口密套有一適段經植菌後之牛樟木塞而形成一菌絲體培養瓶,配合一裝有適量水之塑膠杯容器相承置套設,並令容器內空氣保持流通及維持一定濕度,再藉由牛樟木塞中心設貫孔可釋出瓶內之牛樟芝生長因子氣態純露,俾達到可利於菌絲體滋長及快速誘發子實體長成牛樟菇。The invention relates to a "burdock fruit body and mycelium rapid culture bottle device", in particular to using a mycelium culture bottle to fill the bottle with burdock wood chips and implanting the burdock mycelium, and the bottle mouth is densely sealed. A set of mycelial culture flasks is formed by arranging a suitable burdock of the burdock, and is fitted with a plastic cup container containing a suitable amount of water, and the air in the container is kept in circulation and maintained at a certain humidity, and then the burdock is used. The center hole of the plug can release the gaseous deuterium of the growth factor of the Antrodia camphorata in the bottle, which can be beneficial to the growth of the mycelium and quickly induce the growth of the fruit body into the bovine mushroom.
按,目前現今可為培育生長牛樟芝子實體之菇類的生產設備,一般是將大量之牛樟木段木材以小量分批包裝置放於貨架上,並經機械式清洗桶及自動化過濾設備,以進行清洗、高溫殺菌後,再將段木材分批送入一較大環境空間之密閉培育室內,以倉儲方式將段木材逐一排列置放於該培育室內之培養框架上,使段木材於培育室內所定之溫度、濕度之人工環境中進行牛樟芝子實體之菇類長出至收成;然,此培育生產之過程中,是須經過多道之設備裝置,且每一道設備裝置之流程非連貫一致,且其高溫殺菌斧雖可達到100%完全滅菌,惟植入單一菌或人工製程菌種過程中菌絲體會近親交配,最後會失去活性產生呆滯,難長出優良牛樟子實體,因此,尚未系統化的培育作業方式十分繁雜、瑣碎,勢必耗費工時而提高各項人力成本,又培育室內之環境空間大,溫度及濕度難以在培育過程中查覺 到,一旦培育環境之條件控制不當,勢使影響每一批牛樟芝子實體之菇類品質,造成生產良率不高。According to the present, it is now possible to cultivate the production equipment of the mushroom body of the growth of A. angustifolia. Generally, a large amount of the burdock wood is placed on the shelf in a small amount of the batch packaging device, and the mechanical cleaning bucket and the automatic filtering device are used. After cleaning and high-temperature sterilization, the segmental wood is sent into a closed cultivation room of a large environmental space in batches, and the segmental wood is arranged one by one in the cultivation frame of the cultivation room, so that the segment of wood is in the cultivation room. In the artificial environment with the specified temperature and humidity, the mushroom of the body of the Antrodia camphorata grows to the harvest; however, in the process of cultivating and producing, it is necessary to go through multiple equipments, and the process of each equipment is not consistent. And its high-temperature sterilization axe can achieve 100% complete sterilization, but the mycelium will be intimately mated during the process of implanting single or artificial process bacteria, and finally it will lose its activity and become sluggish, and it is difficult to grow good burdock fruit body. Therefore, it has not been systematic. The cultivation method is very complicated and trivial. It will inevitably take time to improve the labor costs, and cultivate the indoor environment. Degree and humidity are difficult to detect during the cultivation process As soon as the conditions for cultivating the environment are not properly controlled, it will affect the quality of the mushrooms of each batch of A. sylvestris fruit, resulting in low production yield.
本發明人有鑑於此,乃思及創作的意念,遂以多年的經驗加以設計,經多方探討並試作樣品試驗,及多次修正改良,乃推出本發明。The present inventors have invented the present invention in view of the above, and the idea of creation and creation, which has been designed with many years of experience, and has been explored and tested by various parties, and has been modified several times.
本發明之主要目的,乃在提供一種牛樟子實體及菌絲體快速培養瓶裝置,尤指其利用一菌絲體培養瓶內裝填牛樟木屑及植入菌絲體,瓶口密套有一中心設有貫孔之牛樟木塞,即可形成一菌絲體培養瓶,再於木塞表面植設適量牛樟菌,配合一裝有適量水位之塑膠杯容器套設於瓶口承緣倒置,藉由容器內空氣可保持流通及維持一定濕度,使牛樟木塞中心貫孔可釋出培養瓶內發酵後之牛樟芝生長因子氣態純露以利菌絲體滋長及快速誘發子實體成長,達到簡化培育流程而有效降低人力與設備成本。The main object of the present invention is to provide a rapid culture flask device for burdock fruit body and mycelium, in particular to use a mycelium culture bottle to fill burdock wood chips and implant mycelium, and the bottle mouth has a central set. A sirloin stalk with a through hole can form a mycelium culture bottle, and then plant a proper amount of burdock on the surface of the cork, and a plastic cup container with a proper amount of water is placed on the edge of the bottle to be inverted, by the container The internal air can maintain circulation and maintain a certain humidity, so that the central hole of the burdock cork can release the fermentation of the Astragalus sinensis growth factor gaseous pure dew in the culture bottle to facilitate the growth of the mycelium and rapidly induce the growth of the fruit body, thereby simplifying the cultivation process. Effectively reduce labor and equipment costs.
本發明之另一目的,乃在提供一種牛樟子實體及菌絲體快速培養瓶裝置,其利用培養瓶瓶體之下方設組接口係可與一內設有牛樟木屑作為培養基營養供給之補充瓶相連接,令菌絲體培養瓶之瓶內菌絲長滿呈白色狀時,即旋出旋蓋並更換套接補充瓶以提供菌絲體及子實體繼續生長所需之營養源,依此類推,無須再次植菌,以達培育牛樟菌絲體長成菇形子實體之流程作業上更為快速、簡化而利於量產。Another object of the present invention is to provide a rapid culture flask device for burdock fruit body and mycelium, which can be used as a supplementary bottle for providing nutrient supply of burdock wood with a set of interface system under the bottle body of the culture bottle. When the hyphae in the bottle of the mycelial culture flask is full of white, the screw cap is screwed out and the sleeve is replaced to provide the nutrient source for the mycelium and the fruit body to continue growing. By analogy, there is no need to replant the bacteria, so that the process of growing the burdock mycelium into a mushroom-shaped fruit body is faster, simpler and more convenient for mass production.
茲配合圖式將列舉出較佳之實施例,詳細說明本發明之內容如下:如第一圖所示係為本發明之流程圖一(請同時參考第二圖所示),其主要包含下列組件:一培養瓶(10)、一牛樟木塞(11)及一預設有適當容積量之塑膠杯容器(12),培養瓶(10)之一側設一供牛樟木塞(11)作緊密套入之瓶口(101),另一側設一開放組接口(102)與一旋蓋(13)相旋緊封閉蓋合,牛樟木塞(11)係配合瓶口(101)之內徑而設呈適大圓段狀且中心貫設一貫孔(111),一或一以上裝填有牛樟木屑(14)之補充瓶(15)上方開設有一套接口(151)與一套蓋(16)套合,套接口(151)恰可與培養瓶(10)之組接口(102)相套設密合組接銜設;其培養製程步驟流程說明如下:The preferred embodiment will be described with reference to the drawings, and the details of the present invention are as follows: as shown in the first figure, it is a flow chart 1 of the present invention (please refer to the second figure at the same time), which mainly includes the following components. : a culture flask (10), a calf stopper (11) and a plastic cup container (12) pre-set with a suitable volume, and a side of the culture flask (10) is provided with a burdock (11) for tight nesting The bottle mouth (101), the other side is provided with an open group interface (102) and a screw cap (13) is tightly closed and closed, and the burdock plug (11) is matched with the inner diameter of the bottle mouth (101). Suitable for large round segments and continuous through holes (111), one or more filling bottles (15) filled with burdock chips (14) are provided with a set of interfaces (151) and a set of covers (16). The socket (151) can be closely combined with the group interface (102) of the culture bottle (10); the process of the cultivation process is as follows:
一、首先進行菌絲體培養:First, the mycelium culture is first carried out:
(A)先取一培養瓶(10),將適量材積之牛樟木屑(14)經牛樟精油滅菌後裝填於瓶內為培養基,並將牛樟菌菌絲體植入瓶內,再將牛樟木塞(11)上端緣環圍塗設有塗劑,經牛樟精油滅菌過後氣密旋入瓶口(101)中,瓶口(101)與木塞(11)接合處使形成氣密封口,而牛樟木塞(11)下端則凸伸一適段至瓶內,並於牛樟木塞(11)表面植上適量牛樟菌絲體,即完成第一階段菌絲體培養瓶(10)之植菌步驟,此時可將殺一菌棉條(112)暫塞設於木塞貫孔(111)以防止霉菌進入,俟於進行子實體培養時取出;(A) First take a culture flask (10), sterilize the appropriate amount of burdock wood chips (14) through burdock essential oil, fill it into the bottle as a medium, and implant the burdock mycelium into the bottle, and then burdock The top edge of the plug (11) is coated with a coating agent, and after being sterilized by the burdock oil, it is airtightly screwed into the bottle mouth (101), and the mouth of the bottle mouth (101) and the cork (11) are formed to form a gas seal port. The lower end of the burdock (11) protrudes from the appropriate section into the bottle, and the appropriate amount of burdock mycelium is planted on the surface of the burdock (11) to complete the germination step of the first stage mycelium culture flask (10). At this time, the killing tampon (112) can be temporarily plugged in the wooden plug hole (111) to prevent mold from entering, and taken out when the fruit body culture is carried out;
(B)接著,進行第二階段牛樟子實體培養:係先將塑膠杯容器(12)倒入適量的水(121),將植菌後之菌絲體培養瓶(10)上之牛樟木塞(11)貫孔(111)內之殺菌棉條(112)取出,此時並將菌絲體培養瓶(10)瓶口面朝下直接倒放置於塑膠杯容器(12)之杯口承緣上,使瓶口(101)中之牛樟木塞(11)面呈朝下靜置於塑膠杯容器(12)中,藉由塑膠杯容器(12)杯口承緣與菌絲體培養瓶(10)套置周圍處之間隙讓空氣可自由進出及塑膠杯容器(12)內適量之水氣循環,使倒置於塑膠杯容器(12)內空間之牛樟木塞(11)可維持濕度60%以上;及藉由菌絲之好氧性及於生長發酵分解牛樟木屑(14)木質部時會自然產生微量之牛樟芝生長因子氣態純露,令菌絲體培養瓶(10)瓶內之牛樟芝生長因子氣態純露可由牛樟木塞(11)之中心貫孔(111)釋出,以形成有利於菌絲體快速培育滋長及誘發培養子實體成長,並長出牛樟子實體;(B) Next, carry out the second stage of the cultivation of the burdock fruit body: first, pour the plastic cup container (12) into an appropriate amount of water (121), and place the burdock on the mycelium culture flask (10) after the germination (11). The sterilizing sliver (112) in the through hole (111) is taken out, and the mycelium culture bottle (10) is placed face down directly on the cup mouth of the plastic cup container (12), so that The face of the burdock (11) in the mouth of the bottle (101) is placed in the plastic cup container (12) downwards, and is surrounded by the cup mouth of the plastic cup container (12) and the mycelium culture bottle (10). The gap between the air allows the air to enter and exit and the proper amount of water in the plastic cup container (12) to circulate, so that the burdock plug (11) placed in the space inside the plastic cup container (12) can maintain the humidity above 60%; The aerobic property of the silk and the natural fermentation of the xylem of the burdock (14) xylem will naturally produce a trace amount of the gaseous dew of the growth factor of the Antrodia camphorata, so that the growth factor of the Antrodia camphorata in the mycelial culture flask (10) The pure dew can be released from the central through hole (111) of the burdock (11) to form a rapid growth and growth of the mycelium and induce the growth of the cultured fruit body, and grow the burdock fruit. body;
(C)第三階段培養瓶之子實體採收及再培養利用:當上述菌絲體培養瓶(10)長出牛樟子實體後,即可將該菌絲體培養瓶(10)取出採下牛樟子實體之樟菇產物(A),並可視商品化需求,製成膠囊型式或其他機能性之產品,而該採收後之菌絲體培養瓶(10)與牛樟木塞(11)再經牛樟精油殺菌後,即可再回復將其倒置放回該塑膠杯容器(12)杯口承緣套置,繼續進行培養下一批牛樟子實體之養成,依此類推,進行培育新的牛樟子實體長成;藉此,俾達到簡化培養菌絲體及培育子實體之流程而有效降低人力與設備成本。(C) Harvesting and recultivation of the fruit body of the third stage culture bottle: When the mycelium culture flask (10) grows the burdock fruit body, the mycelium culture bottle (10) can be taken out and the burdock fruit body is taken out. The mushroom product (A) can be made into a capsule type or other functional product according to the commercialization demand, and the harvested mycelial culture bottle (10) and the burdock (11) are further subjected to the burdock oil. After sterilizing, it can be returned to the plastic cup container (12) and placed at the edge of the cup. The cultivation of the next batch of burdock fruit body is continued, and the new burdock fruit body is grown. In this way, the process of simplifying the cultivation of the mycelium and the cultivation of the fruiting bodies is achieved, and the labor and equipment costs are effectively reduced.
又,其中,上述菌絲體培瓶體(10)所設之組接口(102),係可與一內充填有牛樟木屑(14)作為培養基之補充瓶(15)相連接(如第三圖所示),當菌絲體培養瓶(10)之瓶內菌絲長滿呈白色狀時(發酵完全),即可隨時旋出旋蓋(13)並套接一補充瓶(15),以提供補充菌絲體及子實體繼續生長所需之營養源,無須再次植菌,如第四圖所示,且又當補充瓶(15)之瓶內菌絲長滿呈白色狀時(發酵完全),即可直接將該補充瓶(15)拆離取下,更換套接新的補充瓶(15)以提供菌絲體及子實體繼續生長所需之營養源,依此類推,俾達到培育牛樟菌絲體長成菇形子實體之流程作業上更為快速、簡化而利於量產。In addition, the group interface (102) provided by the mycelium body (10) can be connected to a supplementary bottle (15) filled with burdock wood chips (14) as a medium (such as the third). As shown in the figure), when the mycelium in the bottle of the mycelial culture flask (10) is full of white (the fermentation is complete), the screw cap (13) can be unscrewed at any time and a supplementary bottle (15) can be sleeved. To provide the nutrient source needed to supplement the mycelium and fruiting bodies to continue to grow, no need to replant the bacteria, as shown in the fourth figure, and when the hyphae in the bottle of the supplemental bottle (15) is full of white (fermentation) Completely), the supplemental bottle (15) can be directly removed and removed, and the new supplementary bottle (15) can be replaced to provide the nutrient source for the mycelium and fruiting bodies to continue to grow, and so on. The process of cultivating the burdock mycelium into a mushroom-shaped fruit body is faster, simpler and more convenient for mass production.
綜上所述,當知本發明確實可為相關產業廣為利用,極具有進步性與新穎性,且本發明於申請前未見公開,已符合專利法之規定,爰依法提出發明專利申請,懇請 鈞局明察,惠准專利,實為感禱。In summary, it is known that the present invention can be widely utilized by related industries, and is extremely progressive and novel, and the present invention has not been disclosed before the application, has complied with the provisions of the Patent Law, and has filed an invention patent application according to law. I would like to ask the bureau to observe and benefit from patents.
惟以上所述者,僅為本發明之其中較佳實施例而已,當不能以之限定本發明實施之範圍;即大凡依本發明申請專利範圍所作之均等變化與修飾,皆應仍屬本發明專利涵蓋之範圍內。The above is only the preferred embodiment of the present invention, and the scope of the present invention is not limited thereto; that is, the equivalent changes and modifications made by the scope of the present invention should still belong to the present invention. Within the scope of the patent.
10...培養瓶10. . . Culture bottle
101...瓶口101. . . Bottle mouth
102...組接口102. . . Group interface
11...牛樟木塞11. . . Burdock
111...貫孔111. . . Through hole
112...殺菌棉條112. . . Sterilized sliver
12...容器12. . . container
121...水121. . . water
13...旋蓋13. . . Screw cap
14...牛樟木屑14. . . Burdock sawdust
15...補充瓶15. . . Supplementary bottle
151...套接口151. . . Socket
16...套蓋16. . . Cover
A...樟菇產物A. . . Mushroom product
第一圖:係為本發明之流程圖一。The first figure is the flow chart 1 of the present invention.
第二圖:係為本發明之菌絲體培養瓶剖面圖。Second figure: is a sectional view of the mycelial culture flask of the present invention.
第三圖:係為本發明之菌絲體培養瓶與補充瓶相連接組合剖面圖。The third figure is a sectional view showing the combination of the mycelium culture bottle and the supplementary bottle of the present invention.
第四圖:係為本發明之流程圖二。The fourth figure is the flow chart 2 of the present invention.
10...培養瓶10. . . Culture bottle
101...瓶口101. . . Bottle mouth
102...組接口102. . . Group interface
11...牛樟木塞11. . . Burdock
111...貫孔111. . . Through hole
12...容器12. . . container
121...水121. . . water
13...旋蓋13. . . Screw cap
14...牛樟木屑14. . . Burdock sawdust
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TW200819041A (en) * | 2006-10-27 | 2008-05-01 | Da-Ming Tsai | Method for cultivating Antrodia camphorate |
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