CN203794894U - Culture dish special for micro-injection of zebrafish embryos - Google Patents
Culture dish special for micro-injection of zebrafish embryos Download PDFInfo
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- CN203794894U CN203794894U CN201420228812.7U CN201420228812U CN203794894U CN 203794894 U CN203794894 U CN 203794894U CN 201420228812 U CN201420228812 U CN 201420228812U CN 203794894 U CN203794894 U CN 203794894U
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- culture dish
- embryo
- groove
- cell board
- semisphere
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Abstract
The utility model relates to a culture dish, in particular to a culture dish special for micro-injection of zebrafish embryos. The culture dish special for micro-injection of the zebrafish embryos aims at solving the problems that the positions of fish eggs can not be fixed, the quantity of the fish eggs can not be counted and the positions of the embryos can not be adjusted due to the fact that the culture dish special for micro-injection of the zebra fish embryos does not exist at present. The upper end face of the box bottom of a culture dish box is provided with at least two square grooves in the length direction. The number of culture unit plates is the same as that of the square grooves. Each square groove is provided with a culture unit plate. Hemispherical embryo grooves are distributed in the upper end face of each culture unit plate in an array mode and are arranged in a 10*10 array mode. The bottom end of each hemispherical embryo groove is provided with a drain hole. A mark number is arranged at each hemispherical embryo groove. The culture dish is used for micro-injection of the zebrafish embryos.
Description
Technical field
The utility model relates to a kind of culture dish, is specifically related to a kind of zebrafish embryo microinjection special culture dish.
Background technology
Zebra fish (Danio rerio, be commonly called as zebrafish) there is strong, the in vitro fertilization and various features such as growth, embryo is transparent, sexual maturation cycle is short, individual little Yi cultivation of fecundity, particularly can carry out large-scale forward gene saturation mutagenesis and screening.These features become one of model animal important in genome times afterwards comprehensively life science.Microinjection is a most frequently used technology in zebra fish research.By injection predetermined substance such as in nucleic acid, protein or certain drug introducing embryo, this normally carries out at the commitment of fetal development.But, do not have at present zebrafish embryo microinjection special culture dish, normally zebrafish embryo is deposited on culture dish, water is removed, be so both unfavorable for embryo's position adjustments, be also unfavorable for the counting of ovum quantity, also be unfavorable for the fixing of ovum position simultaneously, increased the difficulty of injection.
To sum up, there is no at present zebrafish embryo microinjection special culture dish, cause that fish-egg position cannot be fixed, quantity cannot be added up and embryo position cannot regulate.
Utility model content
The utility model does not have zebrafish embryo microinjection special culture dish at present for solving, and causes the problem that fish-egg position cannot be fixed, quantity cannot be added up and embryo position cannot regulate, and then a kind of zebrafish embryo microinjection special culture dish is provided.
The utility model is to solve the problems of the technologies described above the technical scheme of taking to be:
Zebrafish embryo microinjection special culture dish of the present utility model comprises culture dish box body and lid, lid covers on culture dish box body, described culture dish box body is rectangular parallelepiped box body, described special culture dish also comprises that at least two are cultivated cell board, on upper surface at the bottom of culture dish box body box, be processed with along its length at least two square indentations, cultivate the quantity of cell board and the quantity of square indentations is consistent arranges, in each square indentations, be provided with one and cultivate cell board, cultivate matrix on cell board upper surface and be placed with semisphere embryo groove, semisphere embryo's groove is that 10 × 10 matrixes are arranged, the bottom of each semisphere embryo's groove is provided with water vent, each semisphere embryo's groove is provided with label.
The beneficial effects of the utility model are:
Zebrafish embryo microinjection special culture dish of the present utility model is provided with cultivation cell board, cultivate matrix on cell board and be placed with semisphere embryo groove, in each semisphere embryo's groove, be provided with a zebrafish embryo, be conducive to adjust the position of zebrafish embryo, and zebrafish embryo position can be fixed when microinjection, zebrafish embryo can not be touched;
Semisphere embryo's groove of zebrafish embryo microinjection special culture dish of the present utility model is provided with label, in microinjection process, can carry out quantity statistics to zebrafish embryo;
Zebrafish embryo microinjection special culture dish of the present utility model is processed with square indentations, after microinjection, can pull down cultivating cell board, water is flushed to zebrafish embryo in the square indentations of culture dish, zebrafish embryo can be gathered in square indentations, is convenient to collect zebrafish embryo;
Zebrafish embryo microinjection special culture dish of the present utility model is processed with water vent, in the time zebrafish embryo being placed on to semisphere embryo groove with transfer pipet, being convenient to water and being discharged by water vent.
Brief description of the drawings
Fig. 1 is the stereographic map of zebrafish embryo microinjection special culture dish of the present utility model, and Fig. 2 is the vertical view of box body 1 in embodiment one, and Fig. 3 is the vertical view of cultivating cell board 3 in embodiment one, and Fig. 4 is the main sectional view of box body 1 in Fig. 1.
Embodiment
Embodiment one: as shown in Fig. 1～4, the zebrafish embryo microinjection special culture dish of present embodiment comprises culture dish box body 1 and lid 2, lid 2 covers on culture dish box body 1, described culture dish box body 1 is rectangular parallelepiped box body, described special culture dish also comprises that at least two are cultivated cell board 3, on upper surface at the bottom of culture dish box body 1 box, be processed with along its length at least two square indentations 1-1, cultivate the quantity of cell board 3 and consistent setting of quantity of square indentations 1-1, on each square indentations 1-1, be provided with one and cultivate cell board 3, cultivate matrix on cell board 3 upper surfaces and be placed with semisphere embryo groove 3-1, semisphere embryo's groove 3-1 is that 10 × 10 matrixes are arranged, the bottom of each semisphere embryo's groove 3-1 is provided with water vent 3-2, each semisphere embryo's groove 3-1 place is provided with label.
Embodiment two: as shown in Figure 3 and Figure 4, the diameter of present embodiment semisphere embryo groove 3-1 is 1～1.5 millimeter.So design, can cultivate cell board 3 accordingly according to the concrete size Selection of zebrafish embryo.Other composition and annexation are identical with embodiment one.
Embodiment three: as shown in Figure 3 and Figure 4, the diameter of present embodiment semisphere embryo groove 3-1 is 1.1～1.4 millimeters.So design, can cultivate cell board 3 accordingly according to the concrete size Selection of zebrafish embryo.Other composition and annexation are identical with embodiment two.
Embodiment four: as shown in Figure 3 and Figure 4, the diameter of present embodiment semisphere embryo groove 3-1 is 1.2～1.3 millimeters.So design, can cultivate cell board 3 accordingly according to the concrete size Selection of zebrafish embryo.Other composition and annexation are identical with embodiment three.
Embodiment five: as shown in Figure 2, the length of side of present embodiment square indentations 1-1 is 3.5 centimetres, cultivating cell board 3 is square shaped cells plate.So design, is conducive to the collection of zebrafish embryo,
Be conducive to cultivate the fixing of cell board 3 simultaneously.Other composition and annexation are identical with embodiment one, two, three or four.
Embodiment six: as shown in Figure 4, the groove depth of present embodiment square indentations 1-1 is 1.5 millimeters.So design, groove depth is more than or equal to the diameter of zebrafish embryo, and zebrafish embryo can only be positioned at square indentations 1-1, is convenient to collect zebrafish embryo.Other composition and annexation are identical with embodiment five.
Embodiment seven: it is silica gel cell board that present embodiment is cultivated cell board 3.So design, silica gel has certain frictional force, can not scratch zebrafish embryo.Other composition and annexation are identical with embodiment one, two, three, four or six.
Embodiment eight: as shown in Figure 4, the thickness that present embodiment is cultivated cell board 3 is 0.5～0.8 millimeter.So design can be processed semisphere embryo groove 3-1 on cultivation cell board 3.Other composition and annexation are identical with embodiment seven.
Embodiment nine: as shown in Figure 3, on the each cultivation cell board 3 of present embodiment, correspondence is provided with two handle 3-3.So design, it is convenient to extract.Other composition and annexation are identical with embodiment one, two, three, four, six or eight.
Claims (9)
1. a zebrafish embryo microinjection special culture dish, described special culture dish comprises culture dish box body (1) and lid (2), lid (2) covers on culture dish box body (1), described culture dish box body (1) is rectangular parallelepiped box body, it is characterized in that: described special culture dish also comprises that at least two are cultivated cell board (3), on upper surface at the bottom of culture dish box body (1) box, be processed with along its length at least two square indentations (1-1), cultivate consistent setting of quantity of quantity with the square indentations (1-1) of cell board (3), in each square indentations (1-1), be provided with one and cultivate cell board (3), cultivate matrix on cell board (3) upper surface and be placed with semisphere embryo groove (3-1), semisphere embryo's groove (3-1) is that 10 × 10 matrixes are arranged, the bottom of each semisphere embryo's groove (3-1) is provided with water vent (3-2), each semisphere embryo's groove (3-1) locates to be provided with label.
2. zebrafish embryo microinjection special culture dish according to claim 1, is characterized in that: the diameter of semisphere embryo's groove (3-1) is 1～1.5 millimeter.
3. zebrafish embryo microinjection special culture dish according to claim 2, is characterized in that: the diameter of semisphere embryo's groove (3-1) is 1.1～1.4 millimeters.
4. zebrafish embryo microinjection special culture dish according to claim 3, is characterized in that: the diameter of semisphere embryo's groove (3-1) is 1.2～1.3 millimeters.
5. according to the zebrafish embryo microinjection special culture dish described in claim 1,2,3 or 4, it is characterized in that: the length of side of square indentations (1-1) is 3.5 centimetres, cultivating cell board (3) is square shaped cells plate.
6. zebrafish embryo microinjection special culture dish according to claim 5, is characterized in that: the groove depth of square indentations (1-1) is 1.5 millimeters.
7. according to the zebrafish embryo microinjection special culture dish described in claim 1,2,3,4 or 6, it is characterized in that: cultivate cell board (3) for silica gel cell board.
8. zebrafish embryo microinjection special culture dish according to claim 7, is characterized in that: the thickness of cultivating cell board (3) is 0.5～0.8 millimeter.
9. according to the zebrafish embryo microinjection special culture dish described in claim 1,2,3,4,6 or 8, it is characterized in that: the upper correspondence of each cultivation cell board (3) is provided with two handles (3-3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420228812.7U CN203794894U (en) | 2014-05-06 | 2014-05-06 | Culture dish special for micro-injection of zebrafish embryos |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420228812.7U CN203794894U (en) | 2014-05-06 | 2014-05-06 | Culture dish special for micro-injection of zebrafish embryos |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203794894U true CN203794894U (en) | 2014-08-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420228812.7U Expired - Fee Related CN203794894U (en) | 2014-05-06 | 2014-05-06 | Culture dish special for micro-injection of zebrafish embryos |
Country Status (1)
| Country | Link |
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| CN (1) | CN203794894U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107027694A (en) * | 2016-10-19 | 2017-08-11 | 中国水产科学研究院长江水产研究所 | A kind of device and method that soft-shelled turtle ovum is imported for exogenous inducer |
| CN107047396A (en) * | 2017-06-23 | 2017-08-18 | 哈尔滨工业大学 | A kind of visual servo zebra fish injection of heart method |
| CN107966581A (en) * | 2017-11-03 | 2018-04-27 | 同济大学 | A kind of zebrafish embryo microwell plate arranges adding set |
| CN113528583A (en) * | 2021-07-15 | 2021-10-22 | 澳门大学 | Automatic microinjection method, device, system, equipment and storage medium |
-
2014
- 2014-05-06 CN CN201420228812.7U patent/CN203794894U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107027694A (en) * | 2016-10-19 | 2017-08-11 | 中国水产科学研究院长江水产研究所 | A kind of device and method that soft-shelled turtle ovum is imported for exogenous inducer |
| CN107027694B (en) * | 2016-10-19 | 2019-10-01 | 中国水产科学研究院长江水产研究所 | A kind of device and method importing soft-shelled turtle ovum for exogenous inducer |
| CN107047396A (en) * | 2017-06-23 | 2017-08-18 | 哈尔滨工业大学 | A kind of visual servo zebra fish injection of heart method |
| CN107966581A (en) * | 2017-11-03 | 2018-04-27 | 同济大学 | A kind of zebrafish embryo microwell plate arranges adding set |
| CN107966581B (en) * | 2017-11-03 | 2023-08-29 | 同济大学 | Zebra fish embryo microplate arrangement adding device |
| CN113528583A (en) * | 2021-07-15 | 2021-10-22 | 澳门大学 | Automatic microinjection method, device, system, equipment and storage medium |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140827 Termination date: 20150506 |
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| EXPY | Termination of patent right or utility model |