CN201469382U

CN201469382U - Culture dish for in vitro fertilization

Info

Publication number: CN201469382U
Application number: CN200920133819XU
Authority: CN
Inventors: 曾勇; 陈伟滨
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-07-10
Filing date: 2009-07-10
Publication date: 2010-05-19
Anticipated expiration: 2019-07-10

Abstract

The utility model discloses a culture dish for in vitro fertilization, which comprises: a dish body (2) and a plurality of culture units (1) arranged at the bottom of the dish body (2), each culture units (1) comprises: an ovum containing region (11) and a sperm containing region (12) which are concave down relative to the bottom of the dish body (2), and a groove (13) communicating the ovum containing region (11) and the sperm containing region (12), the vertical section of the ovum containing region (11) and the sperm containing region (12) is in an inverted trapezoidal. The culture dish for in vitro fertilization of the utility model can effectively ensure that only the good quality sperm having enough activity can arrive to the ovum, only thus can combine with the ovum, thereby improving the development rate of the fertilized embryo and the success ratio of the embryo transplantation, and finally ensuring that the breeding out posterity has excellent quality, convenient operation, and saved a great deal of nutrient solution.

Description

The culture dish that is used for external fertilization

Technical field

This utility model relates to the equipment that uses in a kind of reproductive medicine test, relates in particular to a kind of vessel that are used for external fertilization.

Background technology

Fertilization is that ovum and sperm are in conjunction with the process that forms germ cell, under the normal condition, the higher mammal fertilization process is carried out in vivo, continuous progress along with medical science, mammiferous sperm and ovum are finished fertilization process in external manually operated environment external fertilization technology is succeedd and development in nearest 20 years rapidly, now reaches its maturity and becomes an important and conventional animal reproduction biotechnology.

The external fertilization technology is significant to the research of animal reproduction mechanism, herding production, medical science and animals on the brink of extinction protection etc.As make the experiment material with mice, rat or rabbit etc., the external fertilization technology can be used for studying the generation of mammal gamete, fertilization and early embryo development mechanism.In the domestic animal breed improvement, the external fertilization technology provides cheap means efficiently for Embryo Production, to making full use of the improved seeds resource, shortens the livestock reproduction cycle, and quickening breed improvement speed etc. has important value.The mankind, the external fertilization technology is some infertility of treatment and overcomes one of important measures of Sex linkage disease.The external fertilization technology still is the indispensable ingredients of modern biotechnology such as mammal embryo transplanting, clone, transgenic and sex controll.

In the external fertilization technology, need to use a kind of culture dish as ovum, sperm fertilization and the further place of growing of germ cell, traditional culture dish is one with cover columnar structured, during cultivation, sperm and ovum are placed in the same culture fluid, the ratio of ovum and sperm combines fertilization so that have the sperm of mobility and fertility with ovum generally at 1: 10000.But in the sperm that adds, a part is the eupyrene sperm with propulsion ability and fertility, some is sperm or the Necrospermia that does not have the propulsion ability simultaneously, these sperms can not make ovum fertilization, also can produce metabolite or toxin simultaneously, ovum and germ cell are had a negative impact.Even if normal sperm, its quality is also uneven, if ovum and numerous sperm are put together simultaneously, then very possible and ovum is bonded to be a second-rate sperm, directly influence fertilization embryo's the developmental rate and the success rate of embryo transfer thus, offspring's quality that breeding is come out is not good.Simultaneously, using traditional culture dish, can combine with ovum smoothly in order to guarantee sperm, the vessel of existing structure need use more culture fluid, and the cost of culture fluid is than higher, this has increased the cost of external fertilization undoubtedly greatly.

The utility model content

The technical problems to be solved in the utility model is to provide a kind of can filter out good sperm combined and can save culture fluid with ovum external fertilization culture dish.

The technical scheme in the invention for solving the above technical problem is, manufactures and designs a kind of culture dish that is used for external fertilization, comprising: the ware body; Its special feature is:

Described ware body bottom is provided with a plurality of cultivations unit, described cultivation unit comprises: recessed ovum disposal area and sperm disposal area, described relatively ware body bottom, and the groove that is communicated with described ovum disposal area and sperm disposal area, the vertical cross-section of described ovum disposal area and sperm disposal area is trapezoidal.Like this, sperm must be swum across groove earlier when arriving the ovum position, can filter out high-quality sperm thus and combine, in addition, only need in two disposal areas and groove, to inject culture fluid and get final product with ovum with enough vigor, reduced the consumption of culture fluid greatly, and two the disposal area for falling the trapezoid cross section, its bottom surface is the plane, the convenient glass capillary that uses carries out suction operation, and help the metabolite come-up, reduce its influence to ovum and germ cell.

Because of the volume of ovum is bigger, the volume of the volumetric ratio sperm disposal area of described ovum disposal area is big.

Described groove can be straight line or curve, and it is trapezoidal that its vertical cross-section also is, and makes things convenient for the experimenter to operate.

The length of described groove is 2 centimetres to 10 centimetres.

Described ware body can be square, and is provided with the linearly cultivation unit of a plurality of grooves in it, and a plurality of cultivations be arranged in parallel between the unit, and in the same side of described a plurality of grooves, described ovum disposal area and sperm disposal area are alternately.

Described ware body also can be rounded, is provided with the sweeping cultivation of a plurality of grooves unit in it, and unitary sperm disposal area and the adjacent next unitary ovum disposal area vicinity of cultivating wherein one are cultivated along circumferential array in a plurality of cultivations unit.

The bottom faces of described ovum disposal area and sperm disposal area is lower than the bottom faces of described groove, and some Necrospermias and unvital sperm will be deposited in the sperm disposal area like this, have significantly reduced the influence to ovum or germ cell.

In addition, culture dish of the present utility model also comprises the top cover that seals described ware body upper opening, and the inwall of this top cover is provided with the raised line that is set up in edge, described ware body top, can prevent to be sticked together with edge, ware body top after top cover is stained with liquid.

Compare with prior art, the culture dish that is used for external fertilization of the present utility model is placed on sperm and ovum respectively in two disposal areas, and connect by groove, the sperm that only has enough forward momentum just can be swum across groove and arrive the ovum disposal area and combine with ovum, thereby the sperm quality that can guarantee to arrive ovum effectively is all better, avoid ropy sperm to combine with ovum easily, simultaneously, minimizing produces harmful effect by metabolite or the toxin that sperm produces to ovum or fertilization ovum, improved fertilization embryo's the developmental rate and the success rate of embryo transfer, and guaranteed that finally the offspring that breeding is come out has better quality; Only need in two disposal areas and groove, to inject culture fluid when cultivating in addition and get final product, can save a large amount of culture fluid; The vertical cross-section of two disposal areas is trapezoidal, its bottom surface is a plane, and when drawing sperm or ovum with glass capillary, this glass capillary is frangibility not like this, handled easily also helps simultaneously the metabolite come-up and reduces harmful effect to ovum or germ cell.

Description of drawings

Fig. 1 is the structural representation of this utility model external fertilization culture dish embodiment one;

Fig. 2 is the structural representation of this utility model external fertilization culture dish embodiment two;

Fig. 3 is perpendicular to the vertical cross section figure of channel direction among the embodiment one;

Fig. 4 is for being parallel to the vertical cross section figure of channel direction among the embodiment one.

The specific embodiment

Be described in further detail below in conjunction with the most preferred embodiment shown in the accompanying drawing.

As depicted in figs. 1 and 2, the culture dish that is used for external fertilization of the present utility model a plurality of cultivations unit 1 of comprising ware body 2 and being arranged on these ware body 2 bottoms.Wherein, described cultivation unit 1 comprises: ovum disposal area 11 that described relatively ware body 2 bottoms are recessed and sperm disposal area 12, and the groove 13 that is communicated with described ovum disposal area 11 and sperm disposal area 12.Like this, only need during cultivation two

disposal areas

11 and 12 and groove 13 in the injection culture fluid get final product, can save many culture fluid, effectively reduced the cultivation cost.Because of the volume of ovum is bigger, the volume of described ovum disposal area 11 is done greatlyyer than the volume of sperm disposal area 12.Described groove 13 both can be straight line, also can be curve, desirable 2 centimetres to 10 centimetres of the length of groove 13 combines with ovum to guarantee effectively to stop the strong inadequately sperm of vigor to arrive ovum disposal area 11, preferred implementation is that the length of described groove 13 is got 3 centimetres.

As shown in Figure 3 and Figure 4, the vertical cross-section of described ovum disposal area 11 and sperm disposal area 12 is trapezoidal, it is trapezoidal that the vertical cross-section of described groove 13 also is, three's bottom surface is the plane, more convenient operation when drawing sperm or ovum with glass capillary, glass capillary is not easy to fracture, and helps the harmful effect of the metabolite come-up minimizing of cell to ovum or germ cell.

As Fig. 4, the bottom faces of described ovum disposal area 11 and sperm disposal area 12 is lower than the bottom faces of described groove 13, Necrospermia or unvital sperm will be deposited in the sperm disposal area 12 like this, have reduced the metabolite that produced by sperm or the toxin adverse effect to ovum or germ cell.

In order to prevent that dust etc. from polluting culture fluid, as Fig. 3 or shown in Figure 4, culture dish of the present utility model also is provided with the top cover 3 of described ware body 2 upper openings of sealing, the inwall of this top cover 3 is provided with many raised lines 31 that are set up in edge, described ware body 2 top, can prevent to be sticked together with edge, ware body 2 top after top cover 3 is stained with liquid.

Culture dish of the present utility model can be made multiple shape:

Among the embodiment one, as Fig. 1, described ware body 2 is square, be provided with the linearly cultivation unit 1 of a plurality of groove 13 in it, be arranged in parallel between a plurality of cultivations unit 1, in the same side of described a plurality of grooves 13, described ovum disposal area 11 and sperm disposal area 12 can make full use of the space alternately.

Among the embodiment two, as Fig. 2, described ware body 2 is rounded, be provided with a plurality of grooves 13 sweeping cultivation unit 1 in it, a plurality of cultivations unit 1 is along circumferential array, wherein a sperm disposal area 12 and adjacent next ovum disposal area 11 vicinities of cultivating unit 1 of cultivating unit 1 can make full use of the space equally.

Claims

1. a culture dish that is used for external fertilization comprises: ware body (2); It is characterized in that:

Described ware body (2) bottom is provided with a plurality of cultivations unit (1), each is cultivated unit (1) and comprising: ovum disposal area (11) and sperm disposal area (12) that described relatively ware body (2) bottom is recessed, and the groove (13) that is communicated with described ovum disposal area (11) and sperm disposal area (12), the vertical cross-section of described ovum disposal area (11) and sperm disposal area (12) is trapezoidal.

2. the culture dish that is used for external fertilization according to claim 1 is characterized in that: the volume of the volumetric ratio sperm disposal area (12) of described ovum disposal area (11) is big.

3. the culture dish that is used for external fertilization according to claim 2 is characterized in that: described groove (13) is straight line or curve, and its vertical cross-section is trapezoidal.

4. according to claim 1, the 2 or 3 described culture dishs that are used for external fertilization, it is characterized in that: the length of described groove (13) is 2 centimetres to 10 centimetres.

5. the culture dish that is used for external fertilization according to claim 4, it is characterized in that: described ware body (2) is square, be provided with the linearly cultivation unit (1) of a plurality of grooves (13) in it, be arranged in parallel between a plurality of cultivations unit (1), in the same side of described a plurality of grooves (13), described ovum disposal area (11) and sperm disposal area (12) are alternately.

6. the culture dish that is used for external fertilization according to claim 4, it is characterized in that: described ware body (2) is rounded, be provided with a plurality of grooves (13) sweeping cultivation unit (1) in it, the sperm disposal area (12) of unit (1) and ovum disposal area (11) vicinity that the adjacent next one is cultivated unit (1) wherein one are cultivated along circumferential array in a plurality of cultivations unit (1).

7. according to claim 1, the 2 or 3 described culture dishs that are used for external fertilization, it is characterized in that: the bottom faces of described ovum disposal area (11) and sperm disposal area (12) is lower than the bottom faces of described groove (13).

8. according to claim 1, the 2 or 3 described culture dishs that are used for external fertilization, it is characterized in that: also comprise the top cover (3) of sealing described ware body (2) upper opening, the inwall of this top cover (3) is provided with the raised line (31) that is set up in edge, described ware body (2) top.