CN103421683A - Sperm competition fertilization dish and application method thereof - Google Patents
Sperm competition fertilization dish and application method thereof Download PDFInfo
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- CN103421683A CN103421683A CN2012101647965A CN201210164796A CN103421683A CN 103421683 A CN103421683 A CN 103421683A CN 2012101647965 A CN2012101647965 A CN 2012101647965A CN 201210164796 A CN201210164796 A CN 201210164796A CN 103421683 A CN103421683 A CN 103421683A
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Abstract
The invention discloses a sperm competition fertilization dish and an application method thereof. The sperm competition fertilization dish comprises a dish body and a dish cover for enclosing an opening of the dish body. A plurality separator plates arranged in the dish body divide the dish cavity into an ovum accommodation zone and a sperm accommodation zone respectively at two sides of the dish body, and Z shape long channel communicating the ovum accommodation zone and the sperm accommodation zone. The invention simulates sperm dissociation path in human natural fertilization, realizes optimization of sperms, effectively guarantees that only high-quality sperms with enough vitality can arrive the and combine with the ovum, thereby raising growth rate of fertilized embryos and success rate of embryo transplantation, and finally guaranteeing excellent quality of the progeny.
Description
Technical field
The present invention relates to a kind of medical appliance, relate in particular to a kind of sperm competition fertilization ware.
Background technology
(In Vitro Fertilization) in vitro fertilization or (external fertilization) refer to that mammiferous sperm and ovum complete the technology of fertilization process in vitro in manually operated environment, English referred to as IVF.Because it is inseparable with embryo transfer technology (ET), again referred to as IVF-ET.In biology, claim test-tube animal (test-tube animal) IVF to the animal obtained after parent.Technology in vitro fertilization is significant to the research of animal reproduction mechanism, herding production, medical science and animals on the brink of extinction protection etc.The indispensable integral parts of modern biotechnology such as technology in vitro fertilization or mammal embryo transplanting, clone, transgenosis and sex control.
In fertilization technology in vitro, need to use a kind of sperm and ovum of supplying to carry out culture dish in vitro fertilization.Be distributed with several holes of independent distribution separately on external fertilization dish used in everyday, in vitro fertilizationly exactly sperm and ovum added in the hole of the ware of being fertilized, the rear cultivation that closes the lid, make sperm and ovum combination.But the fertilization process of state of nature is: sperm need to pass through uterine neck, uterus finally could arrive uterine tube, be that sperm must be through trudging or could arriving uterine tube and be combined with ovum through keen competition, the fertilization process of state of nature is the process of a survival of the fittest.Chinese patent specification sheets CN101451104A discloses a kind of external fertilization dish, by the first accommodating area and the second accommodating area and at least one passage that is communicated with between two accommodating areas, formed, described passage in a tubular form and its top have ventilating pit.External fertilization dish by this structure can filter out good sperm and be combined with ovum, but the structure relative complex of this fertilization ware.
Summary of the invention
The objective of the invention is to overcome the defect of prior art, a kind of sperm competition fertilization ware simple in structure of energy simulating nature fertilization survival of the fittest process is provided.
For achieving the above object, the invention provides a kind of sperm competition fertilization ware, comprise the ware lid of ware body and sealing ware body open-mouth, in described ware body, by a plurality of dividing plates are set, the ware intracoelomic cavity is separated into to ovum accommodating area and the sperm accommodating area that lays respectively at ware body both sides and is communicated with described ovum accommodating area and the zigzag long-channel of sperm accommodating area.
Described dividing plate comprises: the first wash plate be arranged in parallel successively, the second wash plate, the first short dividing plate of the 3rd wash plate and the 4th wash plate and arranged transversely and the second short dividing plate, described the first wash plate, the inner left wall of the first short dividing plate and ware body surrounds described ovum accommodating area, described the 4th wash plate, the right side inwall of the second short dividing plate and ware body surrounds described sperm accommodating area, described the second wash plate is affixed near an end and the ware body inwall of the first short dividing plate, the other end and ware body inwall have certain distance first passage that turns round, described the 3rd wash plate is affixed near an end and the ware body inwall of the second short dividing plate, the other end and ware body inwall have certain distance second passage that turns round.
Described ware body sidewall is higher than described the first wash plate, the second wash plate, the 3rd wash plate and the 4th wash plate, and described the first wash plate, the second wash plate, the 3rd wash plate and the 4th wash plate are higher than the described first short dividing plate and the second short dividing plate.
The height of described ware body is 1 centimetre, and the height of described the first wash plate, the second wash plate, the 3rd wash plate and the 4th wash plate is 0.7 centimetre, and the height of the described first short dividing plate and the second short dividing plate is 0.2 centimetre.
Described the first wash plate, the second wash plate, the 3rd wash plate and the 4th wash plate are straight line dividing plate or S-shaped arc-shaped partition.
A kind of using method of sperm competition fertilization ware is characterized in that comprising the following steps:
Steps A: nutrient solution is injected in ovum accommodating area and sperm accommodating area to the fertilization ware, and described nutrient solution also flows into the zigzag long-channel that is communicated with described ovum accommodating area and sperm accommodating area simultaneously;
Step B: ovum and sperm are inserted respectively in the described ovum accommodating area and sperm accommodating area that is marked with nutrient solution;
Step C: proportion, the mineral oil less than nutrient solution is poured the mineral oil that the fertilization ware makes top covering one deck prevention nutrient solution evaporation of described nutrient solution into;
Step D: described fertilization ware is put into to incubator and cultivate 6-8 hour;
Step e: at first at the sperm of described sperm accommodating area, must do all one can to pass through the zigzag long-channel and could arrive described ovum accommodating area and be combined with ovum.
Preferably, the inside of described incubator keeps the interior environment of 37 ℃ of temperature, humidity 95%, CO2 concentration 5%.
Preferably, the amount that steps A is injected nutrient solution to ovum accommodating area and the sperm accommodating area of fertilization ware should make the height of the liquid level of nutrient solution a little less than the dividing plate that forms the zigzag long-channel.
Preferably, the amount of mineral oil that step C pours the fertilization ware into should make the liquid level of described mineral oil cover the dividing plate that forms the zigzag long-channel.
Compared with prior art, beneficial effect of the present invention is: the present invention has simulated the free path of human body natural's fertilization process sperm, and by the competition between sperm, the sperm that vigor is stronger could be combined with ovum, thereby improves the quality of IVF Embryos.
The accompanying drawing explanation
With reference to accompanying drawing, the present invention will be better understood.Accompanying drawing is as follows:
Fig. 1 is the vertical view of sperm competition fertilization ware of the present invention;
Fig. 2 is the sectional view of sperm competition fertilization ware of the present invention along its A-A line;
Fig. 3 is the sectional view of sperm competition fertilization ware of the present invention along its B-B line;
Fig. 4 is the sectional view of sperm competition fertilization ware of the present invention along its C-C line.
Embodiment
In order to make purpose of the present invention, technical scheme, beneficial effect clearer, below in conjunction with drawings and Examples, the present invention is described in further details.Should be appreciated that specific embodiment described herein, only for explaining the present invention, is not intended to limit the present invention.
Below in conjunction with specific embodiment, realization of the present invention is described in detail:
As shown in Figures 1 to 4, a kind of sperm competition fertilization ware, the ware lid 2 that comprises ware body 1 and sealing ware body open-mouth, be arranged with four wash plates in ware body 1 in parallel, the first short dividing plate 145 and the second short dividing plate 146 of the first wash plate 141, the second wash plate 142, the 3rd wash plate 143 and the 4th wash plate 144 and arranged transversely.The sidewall of ware body 1 higher than described the first wash plate 141, the second wash plate 142, the 3rd wash plate 143 and the 4th wash plate 144, the first wash plates 141, the second wash plate 142, the 3rd wash plate 143 and the 4th wash plate 144 higher than the first short dividing plate 145 and the second short dividing plate 146.Preferably, the height of ware body 1 is 1 centimetre, and the height of the first wash plate 141, the second wash plate 142, the 3rd wash plate 143 and the 4th wash plate 144 is 0.7 centimetre, and the height of the first short dividing plate 145 and the second short dividing plate 146 is 0.2 centimetre.The first wash plate 141, the second wash plate 142, the 3rd wash plate 143 and the 4th wash plate 144 are straight line dividing plate or S-shaped arc-shaped partition, also can adopt other shapes with identical function.
Form passage S1 between the first wash plate 141 and the second wash plate 142, form passage S2 between the second wash plate 142 and the 3rd wash plate 143, form passage S3 between the 3rd wash plate and the 4th wash plate.The second wash plate 142 is affixed near an end and ware body 1 inwall of the first short dividing plate 145, the other end and ware body 1 inwall have certain distance first passage 131 that turns round, the 3rd wash plate 143 is affixed near an end and ware body 1 inwall of the second short dividing plates 146, and the other end and ware body 1 inwall have certain distance second passage 132 that turns round.Passage S1, S2, S3 and first passage 131, second that turns round turns round that passage 132 is common forms zigzag long-channel 13.
For the bubble that prevents the 13 interior generations of zigzag long-channel causes the interior nutrient solution of long-channel 13, cut off, described zigzag long-channel 13 should have certain width, and preferable width is 0.64cm.
The right side inwall that the inner left wall of the short dividing plate 145 of the first wash plate 141, first and ware body 1 surrounds the ovum accommodating area short dividing plate 146 of 11, the four wash plate 144, second and ware body 1 surrounds sperm accommodating area 12.Ovum accommodating area 11 and sperm accommodating area 12 are communicated with by zigzag long-channel 13.
The using method of sperm competition fertilization ware of the present invention comprises the following steps:
Steps A: nutrient solution is injected in ovum accommodating area 11 and sperm accommodating area 12 to the fertilization ware, and described nutrient solution also flows into the zigzag long-channel 13 that is communicated with described ovum accommodating area 11 and sperm accommodating area 12 simultaneously;
Step B: ovum and sperm are inserted respectively in the ovum accommodating area 11 and sperm accommodating area 12 that is marked with nutrient solution;
Step C: proportion, the mineral oil less than nutrient solution is poured the mineral oil that the fertilization ware makes top covering one deck prevention nutrient solution evaporation of described nutrient solution into;
Step D: described fertilization ware is put into to incubator and cultivate 6-8 hour;
Step e: at first at the sperm of described sperm accommodating area 12, must do all one can to pass through zigzag long-channel 13 and could arrive described ovum accommodating area 11 and be combined with ovum.
The amount that steps A is injected nutrient solution to ovum accommodating area 11 and the sperm accommodating area 12 of fertilization ware should make the height of the liquid level of nutrient solution a little less than the dividing plate that forms zigzag long-channel 13.The amount that step C pours the mineral oil of fertilization ware into should make the liquid level of described mineral oil cover the dividing plate that forms zigzag long-channel 13.The inside of incubator described in step D keeps the interior environment of 37 ℃ of temperature, humidity 95%, CO2 concentration 5%.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (9)
1. sperm competition fertilization ware, comprise the ware lid (2) of ware body (1) and sealing ware body open-mouth, it is characterized in that: in described ware body (1), by a plurality of dividing plates (14) are set, the ware intracoelomic cavity is separated into to the ovum accommodating area (11) that lays respectively at ware body both sides and sperm accommodating area (12) and is communicated with described ovum accommodating area (11) and the zigzag long-channel (13) of sperm accommodating area (12).
2. sperm competition as claimed in claim 1 fertilization ware, it is characterized in that: described dividing plate (14) comprising: the first wash plate (141) be arranged in parallel successively, the second wash plate (142), the first short dividing plate (145) of the 3rd wash plate (143) and the 4th wash plate (144) and arranged transversely and the second short dividing plate (146), described the first wash plate (141), the inner left wall of the first short dividing plate (145) and ware body (1) surrounds described ovum accommodating area (11), described the 4th wash plate (144), the right side inwall of the second short dividing plate (146) and ware body (1) surrounds described sperm accommodating area (12), described the second wash plate (142) is affixed near an end and ware body (1) inwall of the first short dividing plate (145), the other end and ware body (1) inwall have certain distance first passage (131) that turns round, described the 3rd wash plate (143) is affixed near an end and ware body (1) inwall of the second short dividing plate (146), the other end and ware body (1) inwall have certain distance second passage (132) that turns round.
3. sperm competition as claimed in claim 2 fertilization ware, it is characterized in that: described ware body (1) sidewall is higher than described the first wash plate (141), the second wash plate (142), the 3rd wash plate (143) and the 4th wash plate (144), and described the first wash plate (141), the second wash plate (142), the 3rd wash plate (143) and the 4th wash plate (144) are higher than the described first short dividing plate (145) and the second short dividing plate (146).
4. sperm competition as claimed in claim 3 fertilization ware, it is characterized in that: the height of described ware body (1) is 1 centimetre, the height of described the first wash plate (141), the second wash plate (142), the 3rd wash plate (143) and the 4th wash plate (144) is 0.7 centimetre, and the height of the described first short dividing plate (145) and the second short dividing plate (146) is 0.2 centimetre.
5. as described as claim 1 to 4 any one claim sperm competition fertilization ware, it is characterized in that: described the first wash plate (141), the second wash plate (142), the 3rd wash plate (143) and the 4th wash plate (144) are straight line dividing plate or S-shaped arc-shaped partition.
6. the using method of a sperm competition as claimed in claim 1 fertilization ware is characterized in that comprising the following steps:
Steps A: nutrient solution is injected in ovum accommodating area (11) and sperm accommodating area (12) to the fertilization ware, and described nutrient solution also flows into the zigzag long-channel (13) that is communicated with described ovum accommodating area (11) and sperm accommodating area (12) simultaneously;
Step B: ovum and sperm are inserted respectively in the described ovum accommodating area (11) and sperm accommodating area (12) that is marked with nutrient solution;
Step C: proportion, the mineral oil less than nutrient solution is poured the mineral oil that the fertilization ware makes top covering one deck prevention nutrient solution evaporation of described nutrient solution into;
Step D: described fertilization ware is put into to incubator and cultivate 6-8 hour;
Step e: must do all one can to pass through zigzag long-channel (13) at the sperm of described sperm accommodating area (12) at first and could arrive described ovum accommodating area (11) and be combined with ovum.
7. the using method of sperm competition fertilization ware as claimed in claim 6, is characterized in that: the interior environment of 37 ℃ of the temperature of inside maintenance of described incubator, humidity 95%, CO2 concentration 5%.
8. the using method of sperm competition as claimed in claim 6 fertilization ware, it is characterized in that: the amount that steps A is injected nutrient solution to ovum accommodating area (11) and sperm accommodating area (12) of fertilization ware should make the height of the liquid level of nutrient solution a little less than the dividing plate of formation zigzag long-channel (13).
9. the using method of sperm competition as claimed in claim 6 fertilization ware, it is characterized in that: the amount that step C pours the mineral oil of fertilization ware into should make the liquid level of described mineral oil cover the dividing plate that forms zigzag long-channel (13).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210164796.5A CN103421683B (en) | 2012-05-25 | 2012-05-25 | Sperm competition fertilization dish and application method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210164796.5A CN103421683B (en) | 2012-05-25 | 2012-05-25 | Sperm competition fertilization dish and application method thereof |
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| Publication Number | Publication Date |
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| CN103421683A true CN103421683A (en) | 2013-12-04 |
| CN103421683B CN103421683B (en) | 2015-06-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210164796.5A Expired - Fee Related CN103421683B (en) | 2012-05-25 | 2012-05-25 | Sperm competition fertilization dish and application method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109468226A (en) * | 2018-11-16 | 2019-03-15 | 浙江大学 | Simulate the device in vitro fertilization and application method in sperm travelling path under natural mode |
| CN110343615A (en) * | 2019-08-27 | 2019-10-18 | 浙江大学 | A kind of essence oophyte combines the culture dish and application method of fertilization outside |
Citations (8)
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|---|---|---|---|---|
| WO1993022421A1 (en) * | 1992-05-01 | 1993-11-11 | Trustees Of The University Of Pennsylvania | Microfabricated sperm handling devices |
| CN201168020Y (en) * | 2007-12-25 | 2008-12-24 | 童先宏 | External fertilization dish |
| CN101457203A (en) * | 2008-12-18 | 2009-06-17 | 曾勇 | External fertilization culture dish and external embryo fertilization method using the same |
| JP2009273385A (en) * | 2008-05-13 | 2009-11-26 | Nipro Corp | Petri dish for in vitro fertilization and method for in vitro fertilization |
| CN201469382U (en) * | 2009-07-10 | 2010-05-19 | 曾勇 | Culture dish for in vitro fertilization |
| CN201634677U (en) * | 2010-02-27 | 2010-11-17 | 张微 | Novel bacterial culture dish |
| CN202322835U (en) * | 2011-12-05 | 2012-07-11 | 戴志俊 | In vitro fertilization culture dish for optimizing sperms |
| CN202744550U (en) * | 2012-05-25 | 2013-02-20 | 曾勇 | Fertilization dish adopting sperm competition |
-
2012
- 2012-05-25 CN CN201210164796.5A patent/CN103421683B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993022421A1 (en) * | 1992-05-01 | 1993-11-11 | Trustees Of The University Of Pennsylvania | Microfabricated sperm handling devices |
| AU677781B2 (en) * | 1992-05-01 | 1997-05-08 | Trustees Of The University Of Pennsylvania, The | Microfabricated sperm handling devices |
| CN201168020Y (en) * | 2007-12-25 | 2008-12-24 | 童先宏 | External fertilization dish |
| JP2009273385A (en) * | 2008-05-13 | 2009-11-26 | Nipro Corp | Petri dish for in vitro fertilization and method for in vitro fertilization |
| CN101457203A (en) * | 2008-12-18 | 2009-06-17 | 曾勇 | External fertilization culture dish and external embryo fertilization method using the same |
| CN201469382U (en) * | 2009-07-10 | 2010-05-19 | 曾勇 | Culture dish for in vitro fertilization |
| CN201634677U (en) * | 2010-02-27 | 2010-11-17 | 张微 | Novel bacterial culture dish |
| CN202322835U (en) * | 2011-12-05 | 2012-07-11 | 戴志俊 | In vitro fertilization culture dish for optimizing sperms |
| CN202744550U (en) * | 2012-05-25 | 2013-02-20 | 曾勇 | Fertilization dish adopting sperm competition |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109468226A (en) * | 2018-11-16 | 2019-03-15 | 浙江大学 | Simulate the device in vitro fertilization and application method in sperm travelling path under natural mode |
| CN109468226B (en) * | 2018-11-16 | 2023-08-18 | 浙江大学 | In-vitro fertilization device simulating sperm swimming path in natural state and application method thereof |
| CN110343615A (en) * | 2019-08-27 | 2019-10-18 | 浙江大学 | A kind of essence oophyte combines the culture dish and application method of fertilization outside |
| CN110343615B (en) * | 2019-08-27 | 2023-05-23 | 浙江大学 | Culture dish for sperm-egg in-vitro combined fertilization and use method |
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| CN103421683B (en) | 2015-06-17 |
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Owner name: ZHOU CANQUAN YIN BIAO CHEN WEIBIN HU XIAODONG Effective date: 20150422 |
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Effective date of registration: 20150422 Address after: 518000 No. 1001 Fu Qiang Road, Shenzhen, Guangdong, Futian District Applicant after: Zeng Yong Applicant after: Zhou Canquan Applicant after: Yin Biao Applicant after: Chen Weibin Applicant after: Hu Xiaodong Address before: 518000 No. 1001 Fu Qiang Road, Shenzhen, Guangdong, Futian District Applicant before: Zeng Yong |
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