CN202482319U - External fertilization culture dish - Google Patents

External fertilization culture dish Download PDF

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Publication number
CN202482319U
CN202482319U CN 201220119757 CN201220119757U CN202482319U CN 202482319 U CN202482319 U CN 202482319U CN 201220119757 CN201220119757 CN 201220119757 CN 201220119757 U CN201220119757 U CN 201220119757U CN 202482319 U CN202482319 U CN 202482319U
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China
Prior art keywords
sperm
ovum
culture dish
disposal area
fertilization
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Expired - Fee Related
Application number
CN 201220119757
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Chinese (zh)
Inventor
蒋彦
曹琴英
李淑贤
李清雪
孟繁玉
宋歌
耿彩平
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Shijiazhuang No4 Hospital
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Shijiazhuang No4 Hospital
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Priority to CN 201220119757 priority Critical patent/CN202482319U/en
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Publication of CN202482319U publication Critical patent/CN202482319U/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The utility model discloses an external fertilization culture dish which comprises a culture dish and a seal cap. The external fertilization culture dish is characterized in that the culture dish is provided with a sperm accommodating area and more than two ovum accommodating areas. A passage is disposed between the sperm accommodating area and each ovum accommodating area. The external fertilization culture dish has the advantages that sperm and ovum can be respectively placed, the sperm of high quality can be screened by simulating the mode of internal fertilization, survival rate of fertilized embryos is increased, the quality of bred descendants is guaranteed, environment of the union of a sperm and an ovum is optimized, and the like.

Description

A kind of petridish in vitro fertilization
Technical field
The utility model belongs to medical science equipment field, especially a kind of petridish in vitro fertilization.
Background technology
(IVF) in vitro fertilization is meant that mammiferous sperm and ovum accomplish the technology of fertilization process in external manually operated environment.In biology, claim test-tube animal (test-tube animal) animal that embryo transfer in vitro fertilization obtains behind the parent.Technology in vitro fertilization is significant to the research of animal reproduction mechanism, herding production, medical science and animals on the brink of extinction protection etc.In the Domestic Animal breed improvement, technology in vitro fertilization provides cheap means efficiently for Embryo Production, to making full use of the improved seeds resource, shortens the livestock reproduction cycle, and quickening breed improvement speed etc. has important value.The mankind, the IVF-ET technology is one of some Infertility of treatment important measures sick with overcoming sex linkage.Technology in vitro fertilization still is the indispensable integral parts of modern biotechnology such as mammal embryo transplanting, clone, transgenic and sex controll.
Usually in artificial insemination, in petridish, put into nutrient solution, sperm and ovum are placed in the petridish accomplish fertilization process then.Need about 50,000 to 100,000 sperms to obtain to be fertilized preferably chance when in vitro fertilization, the human body the time of fertilization only needs a spot of sperm.This is because during internal fertilization, and the sperm that human body penetrates also not all possesses fertility, further ripe growth that also must be in female genital tract, and this process is called capacitation, and the external capacitation of sperm depends on BSA in the nutrient solution in IVF.But in extracorporeal fertilization process, sperm concentration is too high, and quality is uneven, and ovum possibly combine with immature sperm, also therefore produces a lot of disadvantageous effects, and for example, the polyploid incidence increases; May influence embryo's potentiality of development; The lyase that the perforatorium dissolving discharges is unfavorable to ovum and embryo; The oxyradical that Necrospermia produces exerts an influence to ovum etc.
The utility model content
The technical problem that the utility model will solve provides a kind of petridish in vitro fertilization, has sperm and ovum is placed respectively, simulation internal fertilization pattern; Screening high-quality sperm; Improve fertilization embryo's surviving rate, guarantee offspring's quality of breeding, optimize advantages such as ovum and sperm bonded environment.
The technical scheme that the utility model is taked is:
A kind of petridish in vitro fertilization, it comprises culture plate and sealing cover, its improvements are: on culture plate, be provided with a sperm disposal area and plural ovum disposal area, be provided with passage between sperm disposal area and the ovum disposal area.
Wherein, the sperm disposal area is positioned at the middle part of culture plate.
Preferably, the ovum disposal area is 2-5.
Preferred, the ovum disposal area is 4.
Adopt the beneficial effect that technique scheme produced to be:
1. sperm is placed on different zones respectively with ovum; And continuous through passage, simulation internal fertilization pattern has only the high-quality The mature sperm just can swim across passage and combines with ovum; Reached screening high-quality sperm; Reduced the polyploid incidence, improved fertilization embryo's surviving rate, guaranteed that the offspring of breeding has fine qualities.
2. ovum and sperm bonded environment have been optimized, the disadvantageous effect of avoiding Necrospermia that ovum is produced.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the utility model is done further detailed explanation.
Fig. 1 is the structural representation of the utility model;
Fig. 2 is an A-A sectional view among Fig. 1.
Wherein, 1 culture plate; 2 sealing covers; 3 sperm disposal areas; 4 ovum disposal areas; 5 passages.
Embodiment
A kind of petridish in vitro fertilization; It comprises culture plate 1 and sealing cover 2; Its improvements are: on culture plate 1, be provided with a sperm disposal area 3 and plural ovum disposal area 4; Sperm disposal area 3 is groove-like with ovum disposal area 4, is provided with passage 5 between sperm disposal area 3 and the ovum disposal area 4.
Sperm disposal area 3 is positioned at the middle part of culture plate 1.
Ovum disposal area 4 is 2-5, and more preferably ovum disposal area 4 is 4.
During use, inject nutrient solution in sperm disposal area 3 on culture plate 1 and the ovum disposal area 4, in the nutrient solution while admission passage 5; Then sperm and ovum are put into sperm disposal area 3 and ovum disposal area 4 respectively, the sperm with enough vigor is swum over to from sperm disposal area 3 the ovum disposal area 4 and is combined to accomplish fertilization process with ovum, thereby reaches the purpose of screening the high-quality sperm.
Sperm is swum into ovum disposal area 4 and is combined with ovum, the disadvantageous effect of avoiding Necrospermia that ovum is produced.

Claims (4)

1. petridish in vitro fertilization; It comprises culture plate (1) and sealing cover (2); It is characterized in that: on culture plate (1), be provided with a sperm disposal area (3) and plural ovum disposal area (4), be provided with passage (5) between sperm disposal area (3) and ovum disposal area (4).
2. a kind of petridish in vitro fertilization according to claim 1 is characterized in that said sperm disposal area (3) is positioned at the middle part of culture plate (1).
3. a kind of petridish in vitro fertilization according to claim 1 and 2 is characterized in that said ovum disposal area (4) is 2-5.
4. a kind of petridish in vitro fertilization according to claim 3 is characterized in that said ovum disposal area (4) is 4.
CN 201220119757 2012-03-27 2012-03-27 External fertilization culture dish Expired - Fee Related CN202482319U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201220119757 CN202482319U (en) 2012-03-27 2012-03-27 External fertilization culture dish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201220119757 CN202482319U (en) 2012-03-27 2012-03-27 External fertilization culture dish

Publications (1)

Publication Number Publication Date
CN202482319U true CN202482319U (en) 2012-10-10

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CN 201220119757 Expired - Fee Related CN202482319U (en) 2012-03-27 2012-03-27 External fertilization culture dish

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CN (1) CN202482319U (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104388306A (en) * 2014-11-08 2015-03-04 黑龙江省畜牧研究所 Culture dish for external fertilization
CN104611219A (en) * 2015-01-26 2015-05-13 辽宁省计划生育科学研究院 Novel culture dish for fertilization
CN104762192A (en) * 2015-03-26 2015-07-08 浙江星博生物科技有限公司 Bionic device for preferably selecting human sperms and carrying out in-vitro fertilization
CN110042057A (en) * 2019-05-07 2019-07-23 遵义医学院附属医院 It is a kind of for external seminal fluid collecting and the device of Embryo Culture

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104388306A (en) * 2014-11-08 2015-03-04 黑龙江省畜牧研究所 Culture dish for external fertilization
CN104611219A (en) * 2015-01-26 2015-05-13 辽宁省计划生育科学研究院 Novel culture dish for fertilization
CN104611219B (en) * 2015-01-26 2017-01-25 辽宁省计划生育科学研究院 Culture dish for fertilization
CN104762192A (en) * 2015-03-26 2015-07-08 浙江星博生物科技有限公司 Bionic device for preferably selecting human sperms and carrying out in-vitro fertilization
CN110042057A (en) * 2019-05-07 2019-07-23 遵义医学院附属医院 It is a kind of for external seminal fluid collecting and the device of Embryo Culture

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Granted publication date: 20121010

Termination date: 20130327