CN104388306A - Culture dish for external fertilization - Google Patents

Culture dish for external fertilization Download PDF

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Publication number
CN104388306A
CN104388306A CN201410650348.5A CN201410650348A CN104388306A CN 104388306 A CN104388306 A CN 104388306A CN 201410650348 A CN201410650348 A CN 201410650348A CN 104388306 A CN104388306 A CN 104388306A
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CN
China
Prior art keywords
sperm
groove
fertilization
ovum
culture dish
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201410650348.5A
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Chinese (zh)
Inventor
丁丽艳
韩永胜
丁得利
殷元虎
何宝国
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Heilongjiang Institute of Animal Husbandrry
Original Assignee
Heilongjiang Institute of Animal Husbandrry
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Heilongjiang Institute of Animal Husbandrry filed Critical Heilongjiang Institute of Animal Husbandrry
Priority to CN201410650348.5A priority Critical patent/CN104388306A/en
Publication of CN104388306A publication Critical patent/CN104388306A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of a culture dish, and particularly relates to a culture dish for external fertilization. The culture dish comprises a dish body and is characterized in that the inner wall of the dish body is provided with a sperm accommodating groove, a fertilization groove and at least one sperm screening groove; the sperm accommodating groove, the sperm screening groove and the fertilization groove are sequentially communicated with one another through arc-shaped grooves. The technical scheme of the culture dish has the beneficial effects of having a function of washing ovum, avoiding adverse effects caused by abnormal ovum to normal ovum, also having a function of screening sperms and preferably selecting good-quality sperms so as to ensure that the success rate of the external fertilization is increased and finally the bred generation is enabled to have good quality.

Description

A kind of external fertilization culture vessel
Technical field
The present invention relates to culture dish technical field, refer to a kind of external fertilization culture vessel especially.
Background technology
At present, culture dish in vitro fertilization has two kinds: a kind of is plate, and another is the culture dish of ovum service sink and fertilization pond composition.The former does not possess the function of cleaning ovum, although the latter has the service sink of cleaning ovum, only has one for ovum fertilization pond in vitro fertilization.Because the ovum taken out from donor nest has heterogeneity, some maturations, have immature, have overdone, have with clot, have by chocolate cyst liquid pollution etc.These dissimilar ovums are all placed in common fertilization pond, prematurity, overdone and may be had negative impact to the fertilization of mature egg by the ovum that clot and chocolate cyst liquid pollute, and likely make ovum rate in vitro fertilization reduce.
In addition, the quality of numerous sperm is uneven, if ovum and numerous sperm put together simultaneously, then what be probably combined with ovum is a second-rate sperm, the offspring quality of breeding out is thus not good, or directly affects the developmental rate of fertilized embryo and the success ratio of embryo transfer.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of external fertilization culture vessel, while raising rate of fertilization, filters out excellent sperm and ovum combines.
For solving the problems of the technologies described above, embodiments of the invention provide a kind of external fertilization culture vessel, comprise ware body, it is characterized in that, described ware body inwall is provided with sperm storage tank, fertilization groove and at least one sperm screening tank, and described sperm storage tank, sperm screening tank, fertilization groove are communicated with successively by arc groove.Sperm arrives the fertilization groove at ovum place must swim across groove, can filter out the sperm with high-quality thus, sperm quality poor or the sperm of dying on the way then can precipitate in sperm screening tank.
As preferably, described ware body middle part is provided with ovum rinse bath.Ovum is cleaned, avoids the negative impact of abnormal ovum to normal ovum.
As preferably, described depth of groove is 2mm to 5mm, and described sperm storage tank, sperm screening tank, fertilization groove depth are all 2cm to 3.5cm mutually.
As preferably, the ring structure internal diameter that described groove is formed is 10cm to 15cm.
The beneficial effect of technique scheme of the present invention is as follows: the function possessing cleaning ovum, abnormal ovum can be avoided the negative impact of normal ovum, and possess the function that sperm is screened, the sperm that preferred mass is good, improve success ratio in vitro fertilization, and finally ensure that breeding offspring out has more excellent quality.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of external fertilization culture vessel embodiment of the present invention.
Embodiment
For making the technical problem to be solved in the present invention, technical scheme and advantage clearly, be described in detail below in conjunction with the accompanying drawings and the specific embodiments.
The present invention is directed to existing deficiency and a kind of external fertilization culture vessel is provided, as shown in Figure 1, comprise ware body 1, described ware body 1 inwall is provided with sperm storage tank 2, fertilization groove 5 and at least one sperm screening tank 6, described sperm storage tank 2, sperm screening tank 6, fertilization groove 5 is communicated with successively by arc groove 7, described groove 7 degree of depth is 2mm to 5mm, the ring structure internal diameter that described groove 7 is formed is 10cm to 15cm, wherein sperm storage tank 2, sperm screening tank 6, fertilization groove 5 degree of depth is all 2cm to 3.5cm mutually, in addition, two sperm screening tanks 6 are provided with between preferred sperm storage tank 2 and fertilization groove 5, can be good at rejecting second-rate sperm, described ware body 1 middle part is provided with ovum rinse bath 3.
This concrete enforcement possesses the function of cleaning ovum, abnormal ovum can be avoided the negative impact of normal ovum, and possess the function that sperm is screened, the sperm that preferred mass is good, improve success ratio in vitro fertilization, and finally ensure that breeding offspring out has more excellent quality.
The above is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from principle of the present invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims (4)

1. an external fertilization culture vessel, comprises ware body, it is characterized in that, described ware body inwall is provided with sperm storage tank, fertilization groove and at least one sperm screening tank, and described sperm storage tank, sperm screening tank, fertilization groove are communicated with successively by arc groove.
2. a kind of external fertilization culture vessel according to claim 1, is characterized in that, described ware body middle part is provided with ovum rinse bath.
3. a kind of external fertilization culture vessel according to claim 2, is characterized in that, described depth of groove is 2mm to 5mm, and described sperm storage tank, sperm screening tank, fertilization groove depth are all 2cm to 3.5cm mutually.
4. a kind of external fertilization culture vessel according to claim 3, is characterized in that, the ring structure internal diameter that described groove is formed is 10cm to 15cm.
CN201410650348.5A 2014-11-08 2014-11-08 Culture dish for external fertilization Pending CN104388306A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410650348.5A CN104388306A (en) 2014-11-08 2014-11-08 Culture dish for external fertilization

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410650348.5A CN104388306A (en) 2014-11-08 2014-11-08 Culture dish for external fertilization

Publications (1)

Publication Number Publication Date
CN104388306A true CN104388306A (en) 2015-03-04

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410650348.5A Pending CN104388306A (en) 2014-11-08 2014-11-08 Culture dish for external fertilization

Country Status (1)

Country Link
CN (1) CN104388306A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105861423A (en) * 2016-04-19 2016-08-17 湖北省农业科学院畜牧兽医研究所 Making method of operation vessel for preparation of pig ICSI zygotes
CN107177490A (en) * 2017-07-19 2017-09-19 同济大学苏州研究院 One kind is new to pick up ovum device
CN111500457A (en) * 2020-06-03 2020-08-07 厦门市妇幼保健院(厦门市计划生育服务中心) Semen optimization culture dish and using method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101451104A (en) * 2008-12-18 2009-06-10 曾勇 In vitro fertilization culture dish and in vitro embryo fertilization method
CN202322835U (en) * 2011-12-05 2012-07-11 戴志俊 In vitro fertilization culture dish for optimizing sperms
CN202482319U (en) * 2012-03-27 2012-10-10 石家庄市第四医院 External fertilization culture dish
CN202519247U (en) * 2012-04-28 2012-11-07 王怀秀 In-vitro fertilization culture dish
CN202744550U (en) * 2012-05-25 2013-02-20 曾勇 Fertilization dish adopting sperm competition

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101451104A (en) * 2008-12-18 2009-06-10 曾勇 In vitro fertilization culture dish and in vitro embryo fertilization method
CN202322835U (en) * 2011-12-05 2012-07-11 戴志俊 In vitro fertilization culture dish for optimizing sperms
CN202482319U (en) * 2012-03-27 2012-10-10 石家庄市第四医院 External fertilization culture dish
CN202519247U (en) * 2012-04-28 2012-11-07 王怀秀 In-vitro fertilization culture dish
CN202744550U (en) * 2012-05-25 2013-02-20 曾勇 Fertilization dish adopting sperm competition

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105861423A (en) * 2016-04-19 2016-08-17 湖北省农业科学院畜牧兽医研究所 Making method of operation vessel for preparation of pig ICSI zygotes
CN107177490A (en) * 2017-07-19 2017-09-19 同济大学苏州研究院 One kind is new to pick up ovum device
CN111500457A (en) * 2020-06-03 2020-08-07 厦门市妇幼保健院(厦门市计划生育服务中心) Semen optimization culture dish and using method thereof

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Application publication date: 20150304