CN101457203A - External fertilization culture dish and external embryo fertilization method using the same - Google Patents
External fertilization culture dish and external embryo fertilization method using the same Download PDFInfo
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- CN101457203A CN101457203A CN 200810241529 CN200810241529A CN101457203A CN 101457203 A CN101457203 A CN 101457203A CN 200810241529 CN200810241529 CN 200810241529 CN 200810241529 A CN200810241529 A CN 200810241529A CN 101457203 A CN101457203 A CN 101457203A
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- disposal area
- culture dish
- fertilization
- groove
- ovum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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Abstract
The invention discloses an in vitro fertilization culture dish comprising a dish body (1); a first storage area (11) for storing ovum and a second storage area (12) for storing sperms arranged in the dish body (1), wherein the first storage area (11) and the second storage area (12) are communicated by at least a groove (13). Moreover the invention also discloses an vitro fertilization method using said culture dish. The in vitro fertilization culture dish and method of the invention can effectively guarantee only the high quality sperms with enough energy to go to the ovum and possibly combine with the ovum, thereby increasing the development rate of the fertilization embryo and the success rate of the embryo transplantation and finally guaranteeing the propagated progenies to have more excellent quality.
Description
Technical field
The present invention relates to the Apparatus and method for of use in a kind of reproductive medicine test, relate in particular to a kind of external fertilization method that is used for vessel in vitro fertilization and utilizes these vessel to finish.
Background technology
(In Vitro Fertilization) in vitro fertilization or (external fertilization) are meant that mammiferous sperm and ovum finish the technology of fertilization process in external manually operated environment, and English abbreviates IVF as.Because it is inseparable with embryo transfer technology (ET), abbreviates IVF-ET again as.In biology, the animal that embryo transfer in vitro fertilization is obtained behind the parent claims test-tube animal (test-tube animal).The success of this technology is the 1950's, development in nearest 20 years rapidly, now reaches its maturity and becomes an important and conventional animal reproduction biotechnology.
Technology in vitro fertilization is significant to the research of animal reproduction mechanism, herding production, medical science and animals on the brink of extinction protection etc.As make the experiment material with mouse, rat or rabbit etc., technology in vitro fertilization can be used for studying the generation of Mammals gamete, fertilization and early embryo development mechanism.In the domestic animal breed improvement, technology in vitro fertilization provides cheap means efficiently for Embryo Production, to making full use of the improved seeds resource, shortens the livestock reproduction cycle, and quickening breed improvement speed etc. has important value.The mankind, the IVF-ET technology is some Infertility of treatment and overcomes one of important measures of sex linkage disease.Technology in vitro fertilization still is the indispensable integral parts of modern biotechnology such as mammal embryo transplanting, clone, transgenosis and sex control.
In technology in vitro fertilization, need to use a kind of sperm and ovum of supplying to carry out culture dish in vitro fertilization, in the prior art, design that this vessel have nothing special, normally in these vessel, put into nutrient solution, simply sperm and ovum are put together to finish fertilization process then.But we know, the quality of numerous sperms is uneven, if ovum and numerous sperm are put together simultaneously, then very possible and ovum bonded is a second-rate sperm, offspring's quality that breeding is come out is not good, perhaps directly influences fertilization embryo's the developmental rate and the success ratio of embryo transfer.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of external embryo fertilization method that can filter out good sperm and ovum bonded culture dish in vitro fertilization and utilize this culture dish.
The technical scheme that the present invention solves the problems of the technologies described above employing is, manufactures and designs a kind of culture dish in vitro fertilization, comprising: the ware body; In described ware body, be provided with and for first disposal area of placing ovum and can be communicated with by at least one groove between described first disposal area and second disposal area for second disposal area of placing sperm.Sperm must be swum across groove earlier when arriving the ovum position like this, can filter out the high-quality sperm with enough vigor thus.
The length of described groove can be 2 centimetres to 10 centimetres.Preferred implementation is that the length of described groove is 3 centimetres.
Described groove is bending, and the size of ware body can be done smallerly like this.
Described groove can have two, is communicated with described first disposal area and second disposal area respectively in both sides.
The bottom faces of described first disposal area, second disposal area and groove is on the same horizontal plane.
The present invention utilizes the external embryo fertilization method of described culture dish to comprise the steps:
(1) nutrient solution is injected in first disposal area in culture dish and second disposal area, and described nutrient solution also flows in the groove that is communicated with described first disposal area and second disposal area simultaneously;
(2) in being inserted respectively, ovum and sperm have in described first disposal area and second disposal area of nutrient solution;
(3) have only the stronger sperm of those vigor can be, swim over to described first disposal area through described groove and contact with ovum and finish fertilization process from described second disposal area.
The length of described groove is 2 centimetres to 10 centimetres and combines with ovum to guarantee effectively to stop the strong inadequately sperm of vigor to arrive first disposal area.
Compare with prior art, culture dish in vitro fertilization of the present invention is placed on sperm and ovum respectively in two disposal areas, and connect by groove, sperm must have enough vigor just can swim across groove to combine with ovum, thereby the sperm quality that can guarantee to arrive ovum effectively is all better, avoid ropy sperm to combine with ovum easily, thereby improved fertilization embryo's the developmental rate and the success ratio of embryo transfer, and guaranteed that finally the offspring that breeding is come out has better quality.
Description of drawings
Fig. 1 is the structural representation of the present invention's culture dish in vitro fertilization.
Embodiment
Be described in further detail below in conjunction with the most preferred embodiment shown in the accompanying drawing.
As shown in Figure 1, culture dish in vitro fertilization of the present invention comprises ware body 1; Two ends in described ware body 1 are provided with first disposal area 11 and second disposal area 12, are communicated with by at least one groove 13 between described first disposal area 11 and second disposal area 12.
Described first disposal area 11, second disposal area 12 and groove 13 are by surrounding from the upwardly extending perpendicular wall in ware body 1 bottom.Wherein, described first disposal area 11 is bigger, rounded, is used to place ovum, and described second disposal area 12 is less, and ovalize is used to place sperm.
The nutrient solution that is injected during because of experiment is few usually, and includes oily matter in the nutrient solution, disconnects through groove the time for fear of nutrient solution, and the bottom faces of described first disposal area 11, second disposal area 12 and groove 13 is on the same horizontal plane.Among the present invention, described groove 13 is provided with two, is communicated with described first disposal area 11 and second disposal area 12 respectively in the left and right sides.Desirable 2 centimetres to 10 centimetres of the length of groove 13 combines with ovum to guarantee effectively to stop the strong inadequately sperm of vigor to arrive first disposal area 12.Preferred implementation is that the length of described groove 13 is 3 centimetres.Oversize for fear of ware body 1, described groove 13 can be made bending, can make full use of the intravital space of ware.
Utilize the external embryo fertilization method of culture dish of the present invention to comprise the steps:
(1) nutrient solution is injected in first disposal area 11 in culture dish and second disposal area 12, and described nutrient solution also flows in the groove 13 that is communicated with described first disposal area 11 and second disposal area 12 simultaneously;
(2) in being inserted respectively, ovum and sperm have in described first disposal area 11 and second disposal area 12 of nutrient solution;
(3) have only the stronger sperm of those vigor can be from described second disposal area 12, swimming over to described first disposal area 11 through described groove 13 contacts with ovum and finishes fertilization process, can filter out high-quality sperm thus, guarantee quality in vitro fertilization with enough vigor.
Claims (8)
1, a kind of culture dish in vitro fertilization comprises: ware body (1); It is characterized in that:
In described ware body (1), be provided with and for first disposal area (11) of placing ovum and can be communicated with by at least one groove (13) between described first disposal area (11) and second disposal area (12) for second disposal area (12) of placing sperm.
2, culture dish in vitro fertilization according to claim 1 is characterized in that: the length of described groove (13) is 2 centimetres to 10 centimetres.
3, culture dish in vitro fertilization according to claim 2 is characterized in that: the length of described groove (13) is 3 centimetres.
4, according to claim 1,2 or 3 described culture dish in vitro fertilization, it is characterized in that: described groove (13) is bending.
5, according to claim 1,2 or 3 described culture dish in vitro fertilization, it is characterized in that: described groove (13) has two, is communicated with described first disposal area (11) and second disposal area (12) respectively in both sides.
6, according to claim 1,2 or 3 described culture dish in vitro fertilization, it is characterized in that: the bottom faces of described first disposal area (11), second disposal area (12) and groove (13) is on the same horizontal plane.
7, a kind of external embryo fertilization method that utilizes the described culture dish of claim 1 is characterized in that this method comprises the steps:
(1) nutrient solution is injected in first disposal area (11) in culture dish and second disposal area (12), and described nutrient solution also flows in the groove (13) that is communicated with described first disposal area (11) and second disposal area (12) simultaneously;
(2) in being inserted respectively, ovum and sperm have in described first disposal area (11) and second disposal area (12) of nutrient solution;
(3) have only the stronger sperm of those vigor can be, swim over to described first disposal area (11) through described groove (13) and contact with ovum and finish fertilization process from described second disposal area (12).
8, external embryo fertilization method according to claim 7 is characterized in that: the length of described groove (13) is 2 centimetres to 10 centimetres and combines with ovum to guarantee effectively to stop the strong inadequately sperm of vigor to arrive first disposal area (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810241529 CN101457203A (en) | 2008-12-18 | 2008-12-18 | External fertilization culture dish and external embryo fertilization method using the same |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810241529 CN101457203A (en) | 2008-12-18 | 2008-12-18 | External fertilization culture dish and external embryo fertilization method using the same |
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| CN101457203A true CN101457203A (en) | 2009-06-17 |
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| CN 200810241529 Pending CN101457203A (en) | 2008-12-18 | 2008-12-18 | External fertilization culture dish and external embryo fertilization method using the same |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103421683A (en) * | 2012-05-25 | 2013-12-04 | 曾勇 | Sperm competition fertilization dish and application method thereof |
| CN110408540A (en) * | 2019-08-07 | 2019-11-05 | 中国科学院苏州生物医学工程技术研究所 | Embryo's changing liquid automatically culture dish |
| CN113355277A (en) * | 2021-06-10 | 2021-09-07 | 四川大学 | Method for improving implantation success rate of mammal in vitro fertilization-embryo transplantation |
| CN115491301A (en) * | 2022-09-05 | 2022-12-20 | 武汉大学 | Microfluidic chip integrating sperm capacitation, sperm sorting, in-vitro fertilization and embryo culture, and preparation method and application thereof |
-
2008
- 2008-12-18 CN CN 200810241529 patent/CN101457203A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103421683A (en) * | 2012-05-25 | 2013-12-04 | 曾勇 | Sperm competition fertilization dish and application method thereof |
| CN103421683B (en) * | 2012-05-25 | 2015-06-17 | 曾勇 | Sperm competition fertilization dish and application method thereof |
| CN110408540A (en) * | 2019-08-07 | 2019-11-05 | 中国科学院苏州生物医学工程技术研究所 | Embryo's changing liquid automatically culture dish |
| CN113355277A (en) * | 2021-06-10 | 2021-09-07 | 四川大学 | Method for improving implantation success rate of mammal in vitro fertilization-embryo transplantation |
| CN115491301A (en) * | 2022-09-05 | 2022-12-20 | 武汉大学 | Microfluidic chip integrating sperm capacitation, sperm sorting, in-vitro fertilization and embryo culture, and preparation method and application thereof |
| CN115491301B (en) * | 2022-09-05 | 2023-09-15 | 武汉大学 | Microfluidic chip integrating sperm capacitation, sperm sorting, in-vitro fertilization and embryo culture and preparation method and application thereof |
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Open date: 20090617 |