CN104611219B - Culture dish for fertilization - Google Patents
Culture dish for fertilization Download PDFInfo
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- CN104611219B CN104611219B CN201510039207.4A CN201510039207A CN104611219B CN 104611219 B CN104611219 B CN 104611219B CN 201510039207 A CN201510039207 A CN 201510039207A CN 104611219 B CN104611219 B CN 104611219B
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- annular groove
- side wall
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- internal orifice
- groove
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- 230000004720 fertilization Effects 0.000 title claims abstract description 27
- 239000011148 porous material Substances 0.000 claims description 4
- 102000002322 Egg Proteins Human genes 0.000 abstract description 17
- 108010000912 Egg Proteins Proteins 0.000 abstract description 17
- 210000004681 ovum Anatomy 0.000 abstract description 17
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 11
- 230000035935 pregnancy Effects 0.000 abstract description 11
- 239000011664 nicotinic acid Substances 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000686 essence Substances 0.000 description 2
- 206010050208 Teratospermia Diseases 0.000 description 1
- 208000002312 Teratozoospermia Diseases 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/22—Petri dishes
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- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
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- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of human assisted reproduction, in particular to a culture dish used for fertilization. The culture medium is a four-hole dish or an annular dish; a dish body of the culture dish comprises a bottom wall, a main side wall and an auxiliary side wall which are integrally formed; the main side wall and the auxiliary side wall are arranged on the bottom wall; the auxiliary side wall is positioned on an inner side of the main side wall; in the four-hole dish, a hole is formed in each of four corners on an inner side of the auxiliary side wall; in the annular dish, a middle hole is formed in the middle of the auxiliary side wall. Sperms added during fertilization are separated from an ovum by utilizing a bionic principle and good-quality sperms are enabled to swim to the periphery of the ovum for fertilization by utilizing a groove, so that the good-quality embryo rate and the clinical pregnancy rate of in-vitro fertilization can be increased.
Description
Technical field
The present invention relates to Issues of Human Assisted Reproductive Technologies field, specially a kind of culture dish for fertilization.
Background technology
At present, supplementary reproduction use for laboratory has four hole wares and annular two specifications of ware in the culture dish of external fertilization.Adopted
Fereilizing style is all that the certain density sperm after processing is added directly in the hole ware being placed with ovum.Due to all essences
Son can directly contact ovum, thus vigor is weak, of poor quality, or even the probability that teratospermia is fertilized to ovum also increases greatly
Plus, thus being unfavorable for the formation of embryo quality, and affect Clinical Pregnancy Rate in.
Content of the invention
In order to overcome above-mentioned deficiency, it is an object of the invention to provide a kind of fertilization culture dish, by being subject to being conventionally used to
The structure of four hole wares of essence and annular ware improves, to improve Embryo quality and the Clinical Pregnancy Rate in of external fertilization.
The technical scheme is that
A kind of fertilization culture dish, this culture dish is four hole wares or annular ware, the ware body of culture dish include diapire, major side wall and
Secondary side wall, three parts are integrally formed, and major side wall and time side wall are arranged on diapire, and secondary side wall is located at the inner side of major side wall；Four
In the ware of hole, inner side four angle of secondary side wall is respectively equipped with hole；In annular ware, the middle setting interstitial hole of secondary side wall；Wherein, improve
Afterwards the feature of annular ware and after improving the feature of annular ware be one below:
(1) groove having a cruciate flower between four holes of common four hole wares is connected with four holes；
(2) there are one and other four bore dia identical interstitial holes in the middle of four holes of common four hole wares, in the middle of here
Two ring annular grooves: outer annular groove, inner annular groove are had around hole；The inner side of outer annular groove has interior annular
Groove internal orifice, the outside of outer annular groove has outer annular groove collar extension；Outer annular groove passes through inner side with interstitial hole
Annular groove internal orifice connects, and outer annular groove is connected by outer annular groove collar extension with inner annular groove, and outer side ring
Connected in star is connected by four holes of four inner annular groove collar extensions and surroundings again respectively；Outer annular groove, exterior annular are recessed
Horizontally set baffle plate between groove, outer annular groove internal orifice and outer annular groove collar extension are located at the both sides of baffle plate respectively；
(3) there are one and other four bore dia identical interstitial holes in the middle of four holes of common four hole wares, in the middle of here
Three ring annular grooves: outer annular groove, intermediate annular groove, inner annular groove are had around hole；Outer annular groove interior
Side has outer annular groove internal orifice, and the outside of outer annular groove has outer annular groove collar extension；Outer annular groove with
Interstitial hole is connected by outer annular groove internal orifice, and outer annular groove passes through outer annular groove collar extension with intermediate annular groove
Connection；The inner side of inner annular groove has inner annular groove internal orifice, and inner annular groove and intermediate annular groove pass through outer
Side ring connected in star internal orifice UNICOM, and inner annular groove passes through four inner annular groove collar extensions and four Kong Lian of surrounding respectively
Logical；Horizontally set baffle plate between outer annular groove, intermediate annular groove, outside outer annular groove internal orifice and outer annular groove
Mouth is located at the both sides of baffle plate respectively；Inner annular groove internal orifice is located at the outer annular groove internal orifice homonymy of baffle plate；
(4) the major side wall top of annular ware is higher than time side wall top, forms outer shroud, secondary side between major side wall and secondary side wall
It is made up of common annular ware interstitial hole and its one, outside annular groove inside wall, the inner side of annular groove has in annular groove
Mouthful, the outside of annular groove has annular groove collar extension, and annular groove is connected by annular groove internal orifice with annular ware interstitial hole,
Annular groove is connected by annular groove collar extension with the sperm groove of setting on outer shroud；Sperm groove is separated with the other parts of outer shroud；
Annular groove horizontally set baffle plate, annular groove internal orifice and annular groove collar extension are located at the both sides of baffle plate respectively；
(5) the major side wall top of annular ware is higher than time side wall top, forms outer shroud, secondary side between major side wall and secondary side wall
By common annular ware interstitial hole and its outside two ring annular groove inside wall: outer annular groove and inner annular groove are constituted,
The inner side of outer annular groove has annular groove internal orifice, and the outside of outer annular groove has outer annular groove collar extension；Interior
Side ring connected in star is connected by outer annular groove internal orifice with annular ware interstitial hole, and outer annular groove is led to inner annular groove
Cross the connection of outer annular groove collar extension；The outside of inner annular groove has an inner annular groove collar extension, inner annular groove with
On outer shroud, the sperm groove of setting is connected by inner annular groove collar extension；Sperm groove is separated with the other parts of outer shroud；Two rings
Connected in star horizontally set baffle plate, outer annular groove internal orifice and outer annular groove collar extension are located at the both sides of baffle plate, outside respectively
Annular groove collar extension is located at the outer annular groove internal orifice homonymy of baffle plate.
In described (1st) item, bottom portion of groove arc diameter is 1.0mm, and groove top width is 2.0～2.5mm, and groove
Top is concordant with the top in four holes of culture dish, and depth of groove is 5.0～6.0mm.
In described (1st) item, each connectivity part width bottom be 1.0mm, top be 2.0～2.5mm, depth be 5.0～
6.0mm.
Described (2nd), in (3) item, annular groove bottom arc diameter be 1.0mm, annular groove top width be 2.0～
2.5mm, annular groove top and five holes: around four holes and interstitial hole top are concordant, annular groove depth be 5.0～
6.0mm.
Described (2nd), in (3) item, each connectivity part width bottom is 1.0mm, and top is 2.0～2.5mm, and depth is 5.0
～6.0mm.
Described (4th), in (5) item, the vertical interval between major side wall and secondary side wall is 3.0～5.0mm；Annular groove bottom
Portion's arc diameter is 1.0mm, and top width is 2.0～2.5mm, and annular groove top is concordant with interstitial hole top, annular groove
Depth is 3.0～4.0mm.
Described (4th), in (5) item, each logical place width bottom be 1.0mm, top be 2.0～2.5mm, depth be 3.0～
4.0mm.
Described (4th), in (5) item, the sperm groove place outer region connecting with annular groove collar extension is and four hole wares
The suitable space of one pore volume, this region is separated with the other parts of outer shroud.
Advantages of the present invention and beneficial effect are:
The present invention utilizes bionic principle, and the sperm that the time of fertilization is added is separated with ovum, and using groove by matter
Measured sperm is swum over to and is fertilized around ovum, thus being conducive to improving Embryo quality and the clinical pregnancy of external fertilization
Rate.
Brief description
Fig. 1 is the structural representation of one embodiment of the invention.
Fig. 2 is the structural representation of another embodiment of the present invention.
Fig. 3 is the structural representation of further embodiment of this invention.
Fig. 4 is the structural representation of further embodiment of this invention.
Fig. 5 is the structural representation of further embodiment of this invention.
In figure, 1 hole；2 grooves；3 diapires；4 major side wall；5 side walls；6 outer annular groove；7 inner annular groove；In 8
Side ring connected in star internal orifice；9 outer annular groove collar extensions；10 baffle plates；11 outer shrouds；12 annular grooves；13 interstitial holes；14 annular grooves
Collar extension；15 annular groove internal orifices；16 sperm grooves；17 intermediate annular grooves；18 inner annular groove internal orifices；19 inner annular groove
Collar extension.
Specific embodiment
As shown in Fig. 1～Fig. 5, the present invention is fertilization culture dish, including four hole wares and annular two specifications of ware, each specification
All include ware body and ware lid.Described ware body includes diapire 3, major side wall 4 and secondary side wall 5, and three parts are integrally formed, major side wall 4
It is arranged on diapire 3 with secondary side wall 5, secondary side wall 5 is located at the inner side of major side wall 4.In four hole wares, inner side four angle of secondary side wall 5
It is respectively equipped with hole 1.In annular ware, the middle setting interstitial hole 13 of secondary side wall 5.
(1) feature of four hole wares after improving:
1. as shown in figure 1, having the groove 2 of a cruciate flower and four between four holes 1 of common four hole culture dishs
Hole 1 connects, and groove 2 bottom arc diameter is 1.0mm, and groove 2 top width is 2.0～2.5mm, and groove 2 top and culture
The top in four holes 1 of ware is concordant, and groove 2 depth is 5.0～6.0mm.The same bottom of each connectivity part width is 1.0mm, top
For 2.0～2.5mm, depth is similarly 5.0～6.0mm.In order to suitably increase the distance between four holes, (namely sperm is in order to complete
Fertilization needs travelling distance), four pitch-row major side wall 4 can be allowed slightly closer.
The sperm of the present embodiment is added in a hole 1, and ovum is added in the other three hole 1.After cruciate flower groove,
Using the sperm path of cruciate flower groove and particular design, allow the measured sperm of matter to swim over to and be fertilized around ovum, thus having
Beneficial to the Embryo quality improving external fertilization and Clinical Pregnancy Rate in.
2. as shown in Fig. 2 having one and other four bore dia identicals in the middle of four holes 1 of common four hole culture dishs
Interstitial hole 13, has two ring annular grooves: outer annular groove 6, inner annular groove 7, annular groove around this interstitial hole 13
Bottom arc diameter is 1.0mm, and annular groove top width is 2.0～2.5mm, annular groove top and five holes (around four
Individual hole 1 and interstitial hole 13) top is concordant, and annular groove depth is 5.0～6.0mm.The inner side of outer annular groove 6 has inner side
Annular groove internal orifice 8, the outside of outer annular groove 6 has outer annular groove collar extension 9.Outer annular groove 6 and interstitial hole
13 are connected by outer annular groove internal orifice 8, and outer annular groove 6 passes through outer annular groove collar extension 9 with inner annular groove 7
Connection, and inner annular groove 7 is connected by four holes 1 of four inner annular groove collar extensions 19 and surroundings respectively.Each connection
The place same bottom of width is 1.0mm, and top is 2.0～2.5mm, and depth is 5.0～6.0mm.Outer annular groove 6, outer side ring
Horizontally set baffle plate 10 between connected in star 7, outer annular groove internal orifice 8 and outer annular groove collar extension 9 are located at baffle plate 10 respectively
Both sides.Similarly, since interstitial hole 13 and two ring annular grooves need certain space, so four holes 1 of surrounding also will be away from master
Wall 4 is slightly closer.
The ovum of the present embodiment is added in four holes 1, and sperm is added in interstitial hole 13.Using two ring annular grooves
Afterwards, using the sperm path of two ring annular grooves and particular design, allow the sperm of better quality to swim over to and be fertilized around ovum,
Thus being conducive to improving Embryo quality and the Clinical Pregnancy Rate in of external fertilization.
3. as shown in figure 3, having one and other four bore dia identicals in the middle of four holes 1 of common four hole culture dishs
Interstitial hole 13, has three ring annular grooves: outer annular groove 6, intermediate annular groove 17, exterior annular around this interstitial hole 13
Groove 7, annular groove bottom arc diameter be 1.0mm, annular groove top width be 2.0～2.5mm, annular groove top with
Five hole (around four holes 1 and interstitial hole 13) tops are concordant, and annular groove depth is 5.0～6.0mm.Outer annular groove 6
Inner side have outer annular groove internal orifice 8, the outside of outer annular groove 6 has outer annular groove collar extension 9, exterior annular
Inner annular groove internal orifice 18 is had inside groove 7.Outer annular groove 6 passes through outer annular groove internal orifice 8 with interstitial hole 13
Connection, outer annular groove 6 connected by outer annular groove collar extension 9 with intermediate annular groove 17, intermediate annular groove 17 and
Inner annular groove 7 is connected by inner annular groove internal orifice 18, and inner annular groove 7 passes through four exterior annular respectively
Four holes 1 of groove collar extension 19 and surrounding connect.The same bottom of each connectivity part width is 1.0mm, and top is 2.0～2.5mm, depth
For 5.0～6.0mm.Horizontally set baffle plate 10 between outer annular groove 6, intermediate annular groove 17, outer annular groove internal orifice 8
It is located at the both sides of baffle plate 10 with outer annular groove collar extension 9 respectively, and inner annular groove internal orifice 18 is then located at the interior of baffle plate 10
Side ring connected in star internal orifice 8 homonymy.Similarly, since interstitial hole 13 and three ring annular grooves need certain space, so surrounding four
Hole 1 is also slightly closer away from major side wall 4.
The ovum of the present embodiment is added in four holes 1, and sperm is added in interstitial hole 13.Using three ring annular grooves
Afterwards, using the sperm path of three ring annular grooves and particular design, allow the sperm of better quality to swim over to and be fertilized around ovum,
Thus being conducive to improving Embryo quality and the Clinical Pregnancy Rate in of external fertilization.
(2) feature of annular ware after improving:
1. as shown in figure 4, major side wall 4 top of annular ware is higher than time side wall 5 top, between major side wall 4 and secondary side wall 5
Form outer shroud 11, the vertical interval between major side wall 4 and secondary side wall 5 is 3.0～5.0mm；By in commonly annular inside secondary side wall 5
Ware interstitial hole 13 and its one, outside annular groove 12 are constituted, and annular groove 12 bottom arc diameter is 1.0mm, and top width is
2.0～2.5mm, annular groove 12 top is concordant with interstitial hole 13 top, and annular groove 12 depth is 3.0～4.0mm.Annular is recessed
The inner side of groove 12 has annular groove internal orifice 15, and the outside of annular groove 12 has annular groove collar extension 14.Annular groove 12 leads to
Cross annular groove internal orifice 15 to connect with annular ware interstitial hole 13, by the sperm groove of annular groove collar extension 14 and setting on outer shroud 11
16 connections.Sperm groove 16 is the space suitable with a pore volume of four hole wares, and this region is separated with the other parts of outer shroud 11.
It is 1.0mm with interstitial hole 13, the same bottom of outer shroud 11 connectivity part width, top is 2.0～2.5mm, depth is similarly 3.0～
4.0mm.Annular groove 12 horizontally set baffle plate 10, annular groove internal orifice 15 and annular groove collar extension 14 are located at baffle plate 10 respectively
Both sides.
The sperm of the present embodiment is added in the sperm groove 16 on outer shroud 11, and ovum is added in interstitial hole 13.Using ring
After connected in star, using the sperm path of annular groove and particular design, allow the measured sperm of matter to swim over to and be fertilized around ovum,
Thus being conducive to improving Embryo quality and the Clinical Pregnancy Rate in of external fertilization.
2. as shown in figure 5, major side wall 4 top of annular ware is higher than time side wall 5 top, between major side wall 4 and secondary side wall 5
Form outer shroud 11, the vertical interval between major side wall 4 and secondary side wall 5 is 3.0～5.0mm；By in commonly annular inside secondary side wall 5
Ware interstitial hole 13 and its outside two ring annular groove: outer annular groove 6, inner annular groove 7 are constituted, annular groove bottom is justified
Arc dia is 1.0mm, and top width is 2.0～2.5mm, and annular groove top is concordant with interstitial hole 13 top, and annular groove is deep
Spend for 3.0～4.0mm.The inner side of outer annular groove 6 has outer annular groove internal orifice 8, and the outside of outer annular groove 6 is opened
There is outer annular groove collar extension 9, the outside of inner annular groove 7 has inner annular groove collar extension 19.Outer annular groove 6 with
Interstitial hole 13 is connected by outer annular groove internal orifice 8, and is connected with inner annular groove 7 by outer annular groove collar extension 9,
Inner annular groove 7 is passed through inner annular groove collar extension 19 and is connected with the sperm groove 16 of setting on outer shroud 11.Sperm groove 16 be with
The suitable space of one pore volume of four hole wares, this region is separated with the other parts of outer shroud 11.The same bottom of each connectivity part width
Portion is 1.0mm, and top is 2.0～2.5mm, and depth is similarly 3.0～4.0mm.Outer annular groove 6, inner annular groove 7 it
Between horizontally set baffle plate 10, outer annular groove internal orifice 8 and outer annular groove collar extension 9 are located at the both sides of baffle plate 10, outward respectively
Side ring connected in star collar extension 19 is located at outer annular groove internal orifice 8 homonymy of baffle plate 10.
The sperm of the present embodiment is added in the sperm groove 16 on outer shroud 11, and ovum is added in interstitial hole 13.Using two
After ring annular groove, using the sperm path of two ring annular grooves and particular design, the sperm of better quality is allowed to swim over to ovum week
Enclose and be fertilized, thus being conducive to improving Embryo quality and the Clinical Pregnancy Rate in of external fertilization.
Embodiment result shows, the present invention solves conventional art and is unfavorable for the formation of embryo quality, impact Clinical Pregnancy Rate in
The problems such as, Embryo quality and the Clinical Pregnancy Rate in of external fertilization can be improved.
Claims (6)
1. it is characterised in that this culture dish is four hole wares or annular ware, the ware body of culture dish includes bottom to a kind of fertilization culture dish
Wall, major side wall and secondary side wall, three parts are integrally formed, and major side wall and time side wall are arranged on diapire, and secondary side wall is located at master
The inner side of wall；In four hole wares, inner side four angle of secondary side wall is respectively equipped with hole；In annular ware, in the middle of the middle setting of secondary side wall
Hole；
Wherein, the feature of four hole wares is one below:
(1) there are one and other four bore dia identical interstitial holes in the middle of four holes of four hole wares, have around this interstitial hole
Two ring annular grooves: outer annular groove, inner annular groove；The inner side of outer annular groove has in outer annular groove
Mouthful, the outside of outer annular groove has outer annular groove collar extension；It is recessed that outer annular groove and interstitial hole pass through interior annular
Groove internal orifice connects, and outer annular groove is connected by outer annular groove collar extension with inner annular groove, and inner annular groove
Connected by four holes of four inner annular groove collar extensions and surroundings respectively again；Between outer annular groove, inner annular groove
Horizontally set baffle plate, outer annular groove internal orifice and outer annular groove collar extension are located at the both sides of baffle plate respectively；
(2) there are one and other four bore dia identical interstitial holes in the middle of four holes of four hole wares, have around this interstitial hole
Three ring annular grooves: outer annular groove, intermediate annular groove, inner annular groove；In the inner side of outer annular groove has
Side ring connected in star internal orifice, the outside of outer annular groove has outer annular groove collar extension；Outer annular groove is led to interstitial hole
Cross the connection of outer annular groove internal orifice, outer annular groove is connected by outer annular groove collar extension with intermediate annular groove；Outward
The inner side of side ring connected in star has inner annular groove internal orifice, and it is recessed that inner annular groove and intermediate annular groove pass through exterior annular
Groove internal orifice UNICOM, and inner annular groove is connected by four holes of four inner annular groove collar extensions and surroundings respectively；Inner side
Horizontally set baffle plate between annular groove, intermediate annular groove, outer annular groove internal orifice and outer annular groove collar extension are respectively
Both sides positioned at baffle plate；Inner annular groove internal orifice is located at the outer annular groove internal orifice homonymy of baffle plate；
Wherein, the feature of annular ware is one below:
(1) the major side wall top of annular ware is higher than time side wall top, forms outer shroud, secondary inside sidewalls between major side wall and secondary side wall
It is made up of annular ware interstitial hole and its one, outside annular groove, the inner side of annular groove has annular groove internal orifice, and annular is recessed
The outside of groove has annular groove collar extension, and annular groove is connected by annular groove internal orifice with annular ware interstitial hole, annular groove
Connected by annular groove collar extension with the sperm groove of setting on outer shroud；Sperm groove is separated with the other parts of outer shroud；Annular groove
Horizontally set baffle plate, annular groove internal orifice and annular groove collar extension are located at the both sides of baffle plate respectively；
(2) the major side wall top of annular ware is higher than time side wall top, forms outer shroud, secondary inside sidewalls between major side wall and secondary side wall
By annular ware interstitial hole and its outside two ring annular groove: outer annular groove and inner annular groove are constituted, and interior annular is recessed
The inner side of groove has annular groove internal orifice, and the outside of outer annular groove has outer annular groove collar extension；Outer annular groove
Connected by outer annular groove internal orifice with annular ware interstitial hole, outer annular groove passes through interior annular with inner annular groove
Groove collar extension connects；The outside of inner annular groove has inner annular groove collar extension, and inner annular groove and outer shroud are arranged
Sperm groove connected by inner annular groove collar extension；Sperm groove is separated with the other parts of outer shroud；Two ring annular grooves are horizontal
Setting baffle plate, outer annular groove internal orifice and outer annular groove collar extension are located at the both sides of baffle plate respectively, outside inner annular groove
Mouth is located at the outer annular groove internal orifice homonymy of baffle plate.
2. according to the fertilization culture dish described in claim 1 it is characterised in that in the feature (1st) of described four hole wares, (2) item,
Annular groove bottom arc diameter be 1.0mm, annular groove top width be 2.0～2.5mm, annular groove top with five
Hole: around four holes are concordant with interstitial hole top, annular groove depth is 5.0～6.0mm.
3. according to the fertilization culture dish described in claim 1 it is characterised in that in the feature (1st) of described four hole wares, (2) item,
Each connectivity part width bottom is 1.0mm, and top is 2.0～2.5mm, and depth is 5.0～6.0mm.
4. according to the fertilization culture dish described in claim 1 it is characterised in that in the described annular feature (1st) of ware, (2) item,
Vertical interval between major side wall and secondary side wall is 3.0～5.0mm；Annular groove bottom arc diameter is 1.0mm, top width
For 2.0～2.5mm, annular groove top is concordant with interstitial hole top, and annular groove depth is 3.0～4.0mm.
5. according to the fertilization culture dish described in claim 1 it is characterised in that in the described annular feature (1st) of ware, (2) item,
Each connectivity part width bottom is 1.0mm, and top is 2.0～2.5mm, and depth is 3.0～4.0mm.
6. according to the fertilization culture dish described in claim 1 it is characterised in that in the described annular feature (1st) of ware, (2) item,
Sperm groove place outer region with the connection of annular groove collar extension is the space suitable with a pore volume of four hole wares, this region
Separate with the other parts of outer shroud.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510039207.4A CN104611219B (en) | 2015-01-26 | 2015-01-26 | Culture dish for fertilization |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510039207.4A CN104611219B (en) | 2015-01-26 | 2015-01-26 | Culture dish for fertilization |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104611219A CN104611219A (en) | 2015-05-13 |
| CN104611219B true CN104611219B (en) | 2017-01-25 |
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ID=53145873
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202322835U (en) * | 2011-12-05 | 2012-07-11 | 戴志俊 | In vitro fertilization culture dish for optimizing sperms |
| CN202482319U (en) * | 2012-03-27 | 2012-10-10 | 石家庄市第四医院 | External fertilization culture dish |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100075411A1 (en) * | 2008-09-22 | 2010-03-25 | Cecchi Michael D | Specimen manipulation device for micro manipulation and biopsy in assisted reproduction and in vitro fertilization |
| CN204455095U (en) * | 2015-01-26 | 2015-07-08 | 辽宁省计划生育科学研究院 | Novel fertilization culture dish |
-
2015
- 2015-01-26 CN CN201510039207.4A patent/CN104611219B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202322835U (en) * | 2011-12-05 | 2012-07-11 | 戴志俊 | In vitro fertilization culture dish for optimizing sperms |
| CN202482319U (en) * | 2012-03-27 | 2012-10-10 | 石家庄市第四医院 | External fertilization culture dish |
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| CN104611219A (en) | 2015-05-13 |
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