CN108913600B - Microscopic insemination operation dish - Google Patents
Microscopic insemination operation dish Download PDFInfo
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- CN108913600B CN108913600B CN201810933211.9A CN201810933211A CN108913600B CN 108913600 B CN108913600 B CN 108913600B CN 201810933211 A CN201810933211 A CN 201810933211A CN 108913600 B CN108913600 B CN 108913600B
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- dish body
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a microscopic insemination operation dish. In order to overcome the defects that the operation liquid in the conventional microscopic insemination operation dish is heated unevenly, the consumption of the operation liquid is large, waste exists, and the operation liquid is easy to shake out, the technical scheme adopted by the invention comprises that the cross section of the dish body is elliptical, and the bottom wall of the dish body is a plate body with consistent thickness and comprises a liquid accumulation part and a lifting part; the liquid accumulation part is positioned in the middle of the dish body, the outer wall of the liquid accumulation part is flush with the bottom edge of the side wall, the lifting parts are positioned at two sides of the liquid accumulation part, and the outer wall of the lifting parts is higher than the bottom edge of the side wall; the hydrops part is provided with a bump protruding upwards, and the top surface of the bump is inclined to form an operation surface for insemination operation. The advantages are that: the operation liquid can be effectively ensured to be heated uniformly in the insemination process and kept at the insemination environment temperature of about 37 ℃, the occupied space is small, the consumption of the operation liquid can be effectively reduced, the waste of the operation liquid is reduced, and in addition, the shaking of the operation liquid from a dish body can be effectively avoided.
Description
Technical Field
The invention belongs to the field of medical equipment, and particularly relates to a microscopic insemination operation dish used in the operation of single sperm injection in oocyte oocytosol in human assisted reproductive medicine.
Background
The single sperm injection in oocyte egg cytoplasm is a novel auxiliary reproduction technology developed in the 80 s of the 20 th century, and the aim of fertilization is achieved by directly injecting single sperm carried by a microinjection needle into the egg cytoplasm of an egg cell. The micro insemination operation dish is an important dish involved in the operation process and comprises a dish body with an upward opening and a dish cover covered on the dish body.
When in use, the operation liquid is firstly put into a dish body, covered with a dish cover and then put into a constant temperature box with the temperature of about 37 ℃ to be preheated and balanced for 4 hours, so as to create a insemination environment close to the human body environment; during insemination operation, the operation dish is taken out of the incubator and placed on a microscope table surface, and the microscope table surface is provided with a heating device so as to maintain the environment temperature of 37 ℃ for the operation dish; adding sperm and ovum into the operation liquid, fixing ovum with micro ovum holding needle, grabbing sperm with micro injection needle, and injecting sperm into ovum.
Most of the current micro insemination operation dishes are round dishes, and in order to be matched with the microinjection needle with a bent needle point, the bottom wall of the dish is arranged to be integrally inclined. The structure of the micro insemination operation dish has the defects in actual use: firstly, the bottom wall which is obliquely arranged cannot be attached to the microscope table surface, so that the operation liquid is heated unevenly in the insemination operation process, and the temperature of the operation liquid cannot be kept at 37 ℃ possibly, which is unfavorable for fertilization; secondly, the round dish body occupies large space, the consumption requirement of the operation liquid contained in the dish body is large, and waste exists; thirdly, the operating fluid is easy to shake out in the process of carrying and taking.
Disclosure of Invention
The invention provides a microscopic insemination operation dish which can effectively ensure that operation liquid is heated uniformly in the insemination process and kept at the insemination environment temperature of about 37 ℃, and the operation dish occupies small space, so that the consumption of the operation liquid can be effectively reduced, the waste of the operation liquid is reduced, and in addition, the shaking of the operation liquid from the dish body can be effectively avoided.
In order to solve the technical problems, the invention adopts the following technical scheme: the microscopic insemination operation dish comprises a dish body with an upward opening and a dish cover covered on the dish body, wherein the dish body comprises a bottom wall and a side wall, the cross section of the dish body is elliptical, the bottom wall of the dish body is a plate body with consistent thickness, and the bottom wall comprises a effusion part and a lifting part; the liquid accumulation part is positioned in the middle of the dish body, the outer wall of the liquid accumulation part is flush with the bottom edge of the side wall, the lifting parts are positioned at two sides of the liquid accumulation part, and the outer wall of the lifting parts is higher than the bottom edge of the side wall; the hydrops part is provided with a bump protruding upwards, and the top surface of the bump is inclined to one side along the length direction of the dish body to form an operation surface for insemination operation.
The liquid storage space of the dish body is in a step shape, the liquid accumulation part is a main operation area, the operation liquid is mainly located at the liquid accumulation part, the outer wall of the liquid accumulation part is flush with the bottom edge of the side wall, so that after the dish body is placed on a microscope table, the liquid accumulation part can be completely attached to the microscope table, uniform heating is achieved, the operation liquid in the operation area is uniformly heated, and the operation liquid is more stably maintained at about 37 ℃ under the insemination environment temperature.
The micro-ovum holding needle and the micro-injection needle are arranged left and right along the length direction of the dish body so as to be convenient to operate, and the volume of the dish body can be reduced on the premise that the injection operation is not affected by the oval dish body, so that the occupied space of the dish body can be reduced, and the using amount of the contained operating fluid can be reduced. In addition, the arrangement of the lifting part reduces the volume of the operating liquid under the condition of ensuring the depth of the operating liquid in the operating area, further reduces the dosage of the operating liquid, and can effectively avoid easy shaking out of the operating liquid.
In addition, set up the lug of taking the operation face of slope on hydrops portion for cooperate the operation of microinjection needle, when guaranteeing the operation area, reduced the processing degree of difficulty of the ware body, the structure is simpler, and the diapire of the ware body also can keep thickness unanimous, and the heating effect is better.
Furthermore, the side wall of the elliptic dish body is provided with a straight wall section which can be used for labeling and labeling, so that the labeling and labeling are smoother and writing is facilitated.
As an improvement, the bottom wall includes a sloped connecting portion, the liquid accumulation portion and the elevation portion are connected by a connecting portion, and the connecting portion gradually slopes upward from the liquid accumulation portion to the elevation portion. The connecting portion can smoothly connect hydrops portion and portion of raising for the diapire shaping of the ware body is simpler, moreover, and the connecting portion of slope can avoid the ware body diapire to the interference in the operation process of micro ovum needle and microinjection needle, makes insemination operation more smooth and easy.
As a further improvement, the projection of the connecting portion on the horizontal plane is in a curved arc shape, and the connecting portion is curved toward the effusion portion. This setting for hydrops portion is little in the space that occupies on the diapire, has further saved the quantity of operating fluid, and moreover, the connecting portion that the bending set up can adapt to wholly to be oval, both ends are circular dish body structure for dish body structural strength is higher.
Preferably, the included angle between the connecting part and the horizontal plane is alpha, wherein alpha is more than or equal to 10 degrees and less than or equal to 15 degrees. The included angle between the connecting part and the horizontal plane is moderate, if alpha is smaller than 10 degrees, the height difference between the lifting part and the dropsy part is too small, the effect of reducing the consumption of the operation liquid is not obvious, and the processing difficulty of the dish body is increased; if α > 15 °, the connection may interfere with the operation of the needle.
As an improvement, the dish body is of an axisymmetric structure, and the operating surface is positioned on one side of the midpoint in the length direction of the dish body and inclines towards the midpoint. During insemination operation, the operation surface is actually towards the microinjection needle, and the boss is biased towards one side of the dish body, so that the operation surface is positioned at one side of the dish body instead of being right, and more operation space is reserved for operating the microinjection needle with complex operation.
As an improvement, one side edge of the operation surface is connected with the liquid accumulation part. After insemination is completed, fertilized eggs peeled off from the microscopic egg holding needle can smoothly and stably roll onto the hydrops part along the operation surface; in addition, one side of the operation surface is connected with the effusion part, so that the structure of the bottom of the dish body is simpler and the processing is convenient.
As an improvement, the operation surface is an arc surface with the middle part recessed downwards. After insemination is completed, the fertilized eggs peeled from the microscopic egg holding needle can be better and more stably supported by the arc-shaped surface which is downwards concave, and slow rolling of the fertilized eggs is facilitated.
Preferably, the tangential angle of the operation surface is 1 ° to 5 °.
Preferably, the area of the operation surface is 0.8cm to 2 cm. The area of the operation surface is moderate; if the area of the operation surface is smaller than 0.8cm, the operation surface is too small, so that the experimental operation is inconvenient, and fertilized eggs after insemination are stripped from the microscopic egg holding needle and easily deviate from the microscopic egg holding needle to directly fall into the effusion part; if the area of the operation surface is larger than 2cm, the operation surface is too large, the occupied space is large, the operation liquid around the convex blocks is heated unevenly, and the occupied space of the operation liquid is small, namely, the space for accommodating the ovum, the sperm and the fertilized ovum is small.
As an improvement, handles are arranged on the outer walls of the two opposite sides of the dish body, and the two handles are symmetrically arranged along the length direction of the dish body. This setting is convenient for the operator to carry, take the ware body.
Drawings
FIG. 1 is an exploded view of the present invention;
FIG. 2 is a cross-sectional view of the dish of the present invention;
FIG. 3 is a cross-sectional view of the dish of the present invention;
FIG. 4 is a top view of the dish of the present invention;
the figure shows: 1. a dish body; 2. a dish cover; 3. a bottom wall; 31. a liquid accumulation part; 32. a raised portion; 33. a connection part; 4. a sidewall; 5. a bump; 51. an operation surface; 6. a handle.
Detailed Description
The technical solutions of the embodiments of the present invention will be explained and illustrated below with reference to the drawings of the embodiments of the present invention, but the following embodiments are only preferred embodiments of the present invention, and not all embodiments. Based on the examples in the embodiments, other examples that a person skilled in the art would obtain without making any inventive effort fall within the scope of the invention.
A microscopic insemination operation dish shown in fig. 1 comprises a dish body 1 with an upward opening and a dish cover 2 covered on the dish body 1, wherein the cross section of the dish body 1 is elliptical.
Referring to fig. 2 and 3, the dish body 1 includes a bottom wall 3 and a side wall 4, the bottom wall 3 of the dish body 1 is a plate body with a uniform thickness, and the bottom wall 3 includes a liquid accumulation portion 31 and a raised portion 32. The liquid accumulation part 31 is positioned in the middle of the dish body 1, and the outer wall of the liquid accumulation part 31 is flush with the bottom edge of the side wall 4. The raised portions 32 are located on both sides of the liquid accumulating portion 31, and the outer wall of the raised portion 32 is higher than the bottom edge of the side wall 4, so as to form a height difference with the liquid accumulating portion 31.
The liquid accumulation part 31 is provided with a bump 5 protruding upwards, the top surface of the bump 5 is inclined to one side along the length direction of the dish body 1 to form an operation surface 51 for insemination operation, and the added operation liquid is higher than the bump 5.
The bottom wall 3 includes an inclined connecting portion 33, the liquid accumulation portion 31 and the elevation portion 32 are connected by the connecting portion 33, and the connecting portion 33 gradually inclines upward from the liquid accumulation portion 31 toward the elevation portion 32.
The included angle between the connecting part 33 and the horizontal plane is alpha, wherein alpha is more than or equal to 10 degrees and less than or equal to 15 degrees. In this embodiment, α=12°.
As shown in fig. 3 and 4, the connection portion 33 is curved in a projection on a horizontal plane, and the connection portion 33 is curved toward the liquid accumulation portion 31.
The dish body 1 has an axisymmetric structure, and the operation surface 51 is located at one side of a midpoint in the length direction of the dish body 1 and is inclined toward the midpoint. In particular, in this case, the projection 5 is arranged on the left side of the midline of the capsule 1, the operating surface 51 being oriented to the right.
One side of the operation surface 51 is connected to the liquid accumulation portion 31.
The operation surface 51 is an arc surface with a concave middle part.
The tangential angle of the operation surface 51 is 1 ° to 5 °. In this embodiment, the tangential angle of the operating surface 51 is 3 °.
The area of the operation surface 51 is 0.8cm to 2 cm. In this embodiment, the area of the operation surface 51 is 1.2 cm.
The dish body 1 is of an integrally formed structure.
The dish body 1 is a transparent piece.
While the invention has been described in terms of embodiments, it will be appreciated by those skilled in the art that the invention is not limited thereto but rather includes the drawings and the description of the embodiments above. Any modifications which do not depart from the functional and structural principles of the present invention are intended to be included within the scope of the appended claims.
Claims (2)
1. The utility model provides a microscopic insemination operation dish, includes the dish body and the dish lid of lid on the dish body that the opening up, and the dish body includes diapire and lateral wall, its characterized in that: the cross section of the dish body is elliptical, the bottom wall of the dish body is a plate body with consistent thickness, and the bottom wall comprises a liquid accumulation part and a lifting part; the liquid accumulation part is positioned in the middle of the dish body, the outer wall of the liquid accumulation part is flush with the bottom edge of the side wall, the lifting parts are positioned at two sides of the liquid accumulation part, and the outer wall of the lifting parts is higher than the bottom edge of the side wall; the liquid accumulation part is provided with an upward protruding block, and the top surface of the protruding block is inclined to one side along the length direction of the dish body to form an operation surface for insemination operation; the bottom wall comprises an inclined connecting part, the effusion part is connected with the lifting part through the connecting part, and the connecting part gradually inclines upwards from the effusion part to the lifting part; the projection of the connecting part on the horizontal plane is in a curved arc shape, and the connecting part is curved towards the effusion part; the included angle between the connecting part and the horizontal plane is alpha, wherein alpha is more than or equal to 10 degrees and less than or equal to 15 degrees; the dish body is of an axisymmetric structure, and the operation surface is positioned on one side of the midpoint in the length direction of the dish body and inclines towards the midpoint; one side edge of the operation surface is connected with the liquid accumulation part; the operation surface is an arc surface with the middle part recessed downwards; the tangential angle of the operation surface is 1-5 degrees; the area of the operation surface is 0.8cm to 2 cm.
2. A micro insemination cuvette according to claim 1, wherein: handles are arranged on the outer walls of the two opposite sides of the dish body, and the two handles are symmetrically arranged along the length direction of the dish body.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810933211.9A CN108913600B (en) | 2018-08-16 | 2018-08-16 | Microscopic insemination operation dish |
| PCT/CN2019/097386 WO2020034815A1 (en) | 2018-08-16 | 2019-07-24 | Micro-insemination operation container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810933211.9A CN108913600B (en) | 2018-08-16 | 2018-08-16 | Microscopic insemination operation dish |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108913600A CN108913600A (en) | 2018-11-30 |
| CN108913600B true CN108913600B (en) | 2023-06-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810933211.9A Active CN108913600B (en) | 2018-08-16 | 2018-08-16 | Microscopic insemination operation dish |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN108913600B (en) |
| WO (1) | WO2020034815A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108913600B (en) * | 2018-08-16 | 2023-06-27 | 杭州德适生物科技有限公司 | Microscopic insemination operation dish |
| CN114703061B (en) * | 2022-06-07 | 2022-08-16 | 中国人民解放军总医院第六医学中心 | Be used for cell culture to use multi-functional intelligent cell culture dish |
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| JPH0582299U (en) * | 1992-04-17 | 1993-11-09 | 住友ベークライト株式会社 | Culture instrument |
| CN2822540Y (en) * | 2005-04-28 | 2006-10-04 | 尼普洛株式会社 | Sperm sorting vessel |
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| US20100075411A1 (en) * | 2008-09-22 | 2010-03-25 | Cecchi Michael D | Specimen manipulation device for micro manipulation and biopsy in assisted reproduction and in vitro fertilization |
| JP5990403B2 (en) * | 2012-05-31 | 2016-09-14 | 株式会社 ジャパン・ティッシュ・エンジニアリング | Culture dish and culture kit |
| EP2931871A4 (en) * | 2012-12-11 | 2016-07-20 | Pall Technology Uk Ltd | Recipient for cell cultivation |
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| CN106479888A (en) * | 2015-08-31 | 2017-03-08 | 赛默飞世尔（上海）仪器有限公司 | Culture dish |
| CN208883903U (en) * | 2018-08-16 | 2019-05-21 | 杭州德适生物科技有限公司 | A kind of microscopic insemination operation ware |
| CN108913600B (en) * | 2018-08-16 | 2023-06-27 | 杭州德适生物科技有限公司 | Microscopic insemination operation dish |
-
2018
- 2018-08-16 CN CN201810933211.9A patent/CN108913600B/en active Active
-
2019
- 2019-07-24 WO PCT/CN2019/097386 patent/WO2020034815A1/en active Application Filing
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| EP0124212A1 (en) * | 1983-05-02 | 1984-11-07 | Becton Dickinson and Company | Petri dish |
| JPH0582299U (en) * | 1992-04-17 | 1993-11-09 | 住友ベークライト株式会社 | Culture instrument |
| CN2822540Y (en) * | 2005-04-28 | 2006-10-04 | 尼普洛株式会社 | Sperm sorting vessel |
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| CN104651221A (en) * | 2013-11-21 | 2015-05-27 | 上海交通大学医学院附属第九人民医院 | Micro-insemination culture dish |
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Also Published As
| Publication number | Publication date |
|---|---|
| WO2020034815A1 (en) | 2020-02-20 |
| CN108913600A (en) | 2018-11-30 |
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Inventor after: Song Ning Inventor after: Xiong Xiaoping Inventor after: Bai Zhixing Inventor after: Wu Chaoyu Inventor after: Zheng Zhong Inventor before: Song Ning Inventor before: Lv Qifeng Inventor before: Xiong Xiaoping Inventor before: Bai Zhixing Inventor before: Wu Chaoyu Inventor before: Zheng Zhong |
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