CN219621189U - Ceiling device for wall-attached culture of floating cells - Google Patents
Ceiling device for wall-attached culture of floating cells Download PDFInfo
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- CN219621189U CN219621189U CN202320419147.9U CN202320419147U CN219621189U CN 219621189 U CN219621189 U CN 219621189U CN 202320419147 U CN202320419147 U CN 202320419147U CN 219621189 U CN219621189 U CN 219621189U
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Abstract
The utility model provides a ceiling device for wall-attached culture of floating cells, which comprises a ceiling plate and a base, wherein one side of the ceiling plate is provided with a plurality of placing grooves, and floating cells are placed in the placing grooves; a placing cavity with an upward opening is arranged in the base, and the ceiling plate is arranged on the side of the opening of the placing cavity so as to cover the opening of the placing cavity; wherein, place intracavity and be suitable for placing culture solution and float the cell, the standing groove is located the smallpox plate orientation place intracavity one side and with the culture solution contact, so that place intracavity float the cell and get into in the standing groove, in order to carry out spacingly to float the cell, prevent that it from removing in order to observe conveniently.
Description
Technical Field
The utility model relates to the technical field of biomedical experimental devices, in particular to a ceiling device for wall-attached culture of floating cells.
Background
Adult stem cells are taken as a stem cell source which can be obtained by self and transplanted, clinical application and research of the adult stem cells relate to tissues of various organs, and the adipose-derived stem cells are paid attention to at present, because the adipose tissues have the characteristics of wide sources, easy acquisition, small damage and the like, and mature adipose cells can also be dedifferentiated and converted into stem cells in the in vitro culture process.
The prior art discloses a cell culture dish, including a plurality of culture holes, be provided with the exchange district between the culture hole that needs to exchange, set up between each culture hole and the exchange district and exchange the board, exchange the board on open and have the exchange hole, exchange the hole and allow the liquid between different culture holes to contain its composition and flow each other.
However, in the cell culture dish, the culture solution is provided in the culture well to culture the cells provided therein, and when the directly isolated in-vivo mature adipocytes are placed in the culture solution to be cultured, the culture dish floats on the liquid surface and is difficult to observe, so that the cell culture dish cannot be applied to the culture observation of the mature adipocytes.
Disclosure of Invention
Therefore, the present utility model has been made to solve the problems of the prior art, in which a culture solution is provided in a culture well to culture cells provided therein, and in which mature adipocytes in a body directly separated from each other are not easily observed due to floating on a liquid surface when they are placed in the culture solution to be cultured, and thus the cell culture dish is not suitable for observation of culture of mature adipocytes.
To this end, the utility model provides a ceiling device for use in the adherent culture of floating cells, comprising:
a plurality of placing grooves are formed in one side of the ceiling plate, and floating cells are placed in the placing grooves;
the base is internally provided with an upward opening placing cavity, and the ceiling plate is arranged on the opening side of the placing cavity to cover the opening of the placing cavity;
the placing cavity is internally suitable for placing culture solution and floating cells, and the placing groove is formed in one side of the ceiling plate facing the placing cavity and is in contact with the culture solution, so that the floating cells in the placing cavity enter the placing groove.
Further, the placing groove is a quarter spherical groove.
Further, two adjacent side walls of the placing groove are connected with each other.
Further, the diameter of the spherical groove is 180-220 microns.
Furthermore, the base is suitable for being placed in an external culture dish, and a plurality of through grooves are formed in the placement cavity in the base, so that external culture solution and floating cells enter the placement cavity.
Further, the through slot opening is lower than the ceiling plate.
Further, the bottom of the base is provided with an opening.
Further, the height of the base is lower than 1cm.
Further, an opening is arranged on the placing cavity in the base, and a sealing cover is arranged on the opening.
The ceiling device for the wall-attached culture of floating cells has the following advantages:
1. the utility model provides a ceiling device for wall-attached culture of floating cells, which comprises a ceiling plate and a base, wherein one side of the ceiling plate is provided with a plurality of placing grooves, and floating cells are placed in the placing grooves; a placing cavity with an upward opening is arranged in the base, and the ceiling plate is arranged on the side of the opening of the placing cavity so as to cover the opening of the placing cavity; the placing cavity is internally suitable for placing culture solution and floating cells, and the placing groove is formed in one side of the ceiling plate facing the placing cavity and is in contact with the culture solution, so that the floating cells in the placing cavity enter the placing groove.
The ceiling device for floating cell wall-attached culture of this structure, the smallpox plate is close to one side of base and is provided with a plurality of standing grooves, and the inside mature adipocytes that is arranged in of standing groove to place to float in the culture solution to carry out spacingly to mature adipocytes, prevent that it from removing in order to observe conveniently.
2. The utility model provides a ceiling device for wall-attached culture of floating cells, which is characterized in that six square through grooves are formed in the side wall of a base, when culture solution is injected into a culture dish, the culture solution enters the base through the six square through grooves until the culture solution is completely filled in the base and submerges a ceiling plate, then a pipette is inserted into the base through square through grooves around the base and is arranged below the ceiling plate, fresh separated mature fat cells in the pipette are injected into the culture solution below the ceiling plate, the mature fat cells float close to the ceiling plate, and occupy the inside of a placing groove on the ceiling plate according to different cell diameter distribution, so that the limit of the mature fat cells is realized, and the observation of the mature fat cells is facilitated.
3. The utility model provides a ceiling device for wall-attached culture of floating cells, wherein placing grooves are uniformly arranged on the surface of a ceiling plate, and the placing grooves are in the shape of quarter spherical grooves recessed from the surface of the ceiling plate to the inside of the surface of the ceiling plate.
4. The ceiling device for the wall-attached culture of floating cells is simple in structure, easy to mold and produce and convenient to adapt to various conventional cell culture vessels.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of the structure of a ceiling device for floating cell attachment culture provided in an embodiment of the present utility model;
FIG. 2 is a schematic diagram of the operation of a ceiling apparatus for use in floating cell attachment culture provided in an embodiment of the present utility model;
FIG. 3 is a schematic view showing another construction of a ceiling apparatus for floating cell attachment culture according to an embodiment of the present utility model;
reference numerals illustrate:
1-ceiling plate;
2-a placing groove;
3-adipocytes;
4-a base;
5-through grooves;
6, sealing the cover;
7-pipettor.
Detailed Description
The following description of the embodiments of the present utility model will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the utility model are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In addition, the technical features of the different embodiments of the present utility model described below may be combined with each other as long as they do not collide with each other.
Examples
Mature adipocytes are adherent adipocytes which contain a large number of cells with lighter lipid density, are helpful for clinical intervention of obesity and related diseases for intensive research, and most of the adipocytes used in vitro cell experiments are induced to differentiate, and the cell states of the adipocytes are different from those of mature adipocytes in vivo, so that the experimental results are often far from in vivo application. Directly isolated mature adipocytes in vivo are difficult to observe because they float on the fluid surface.
The embodiment provides a ceiling device for floating cell wall-attached culture, which is used for floating wall-attached culture of mature adipocytes so as to facilitate observation of the mature adipocytes.
As shown in fig. 1, the ceiling device for the adherent culture of floating cells provided in this embodiment comprises a ceiling plate 1 and a base 4, wherein the base 4 is provided in a cylindrical shape with upper and lower openings, a placement cavity is formed inside the base 4, and the ceiling plate 1 is fixedly connected to the upper side of the base 4 by means of adhesion or the like, thereby realizing the closing of the upper opening of the base 4, so as to prevent the fat cells inside the placement cavity of the base 4 from flowing out from the upper part during the culture.
The ceiling plate 1 in this embodiment is made of transparent polystyrene, and one side of the ceiling plate 1 near the base 4 is provided with a plurality of placement grooves 2, the inside of the placement grooves 2 is used for placing mature adipocytes floating in a culture solution (described below) to limit the mature adipocytes, and prevent them from moving to facilitate observation.
Further, as shown in fig. 1, the placement grooves 2 are uniformly arranged on the surface of the ceiling plate 1, and the placement grooves 2 are in the shape of quarter spherical grooves recessed from the surface of the ceiling plate 1 to the inside of the surface of the ceiling plate 1, and as the mature fat is mostly spherical, the placement grooves 2 are in the shape of quarter spherical grooves, so that the mature fat can be well attached to the surface of the mature fat, and the mature fat can be well limited.
Furthermore, the groove walls between the adjacent placing grooves 2 are mutually connected, i.e. no additional connection part exists between the adjacent placing grooves 2, so that the placing grooves 2 can be arranged on the surface of the ceiling plate 1 as much as possible, and more fat cells can be limited and observed.
Further, the diameter of the placement groove 2 is between 180 and 220 micrometers, and the preferred embodiment is 200 micrometers.
It will be appreciated that the edge portion of the ceiling board 1 is not provided with the placement groove 2 as shown in fig. 1, so as to facilitate bonding of the edge portion of the ceiling board 1 with the upper edge portion of the base 4.
In other embodiments, the ceiling board 1 and the base 4 may be arranged in other shapes, for example, the base 4 is arranged in a cube, and the ceiling board 1 is arranged in a square. It should be understood that ceiling plate 1 should cover the upper part of base 4 entirely so as to close the opening at the upper end of base 4, to prevent fat cells inside base 4 from flowing out from the upper part during cultivation.
As shown in fig. 2, when observing the adipocytes 3 by the ceiling device for the wall-attached culture of floating cells provided in this embodiment, the base 4 is fixed at the bottom of the external culture dish by means of bonding or other connection means, then the culture solution is injected into the culture dish, and the base 4 is fixed at the bottom of the external culture dish by means of bonding or other connection means, so that the base 4 can be prevented from floating in the culture solution, and the ceiling plate 1 connected with the base 4 is prevented from shaking, which is not beneficial to observing the adipocytes 3 in the placement groove 2 in the ceiling plate 1, wherein the height of the base 4 should be smaller than the height in the culture dish, and the height of the base 4 should be as low as 1cm according to the working distance of different microscope objectives.
When observing the adipocytes 3, as shown in fig. 2, after the base 4 is fixed at the bottom of an external culture dish in a connection manner such as bonding, and the liquid level of the culture solution in the culture dish is higher than that of the ceiling plate 1 above the base 4, six square through grooves 5 (only 3 are shown in fig. 1) are arranged on the side wall of the base 4, when the culture solution is injected into the culture dish, the culture solution enters the base 4 through the six square through grooves 5 until the culture solution is completely filled in the base 4 and submerges the ceiling plate 1, then the freshly separated mature adipocytes 3 in the liquid transfer device 7 are injected into the culture solution below the ceiling plate 1 through the square through grooves 5 around the base 4, the mature adipocytes 3 float close to the ceiling plate 1, and occupy the inside of the placement groove 2 on the ceiling plate 1 according to different cell diameter distributions, so that the mature adipocytes 3 can be conveniently limited, and the mature adipocytes 3 can be observed. Wherein, the bottom of standing groove 2 sets up thinness to make things convenient for the microscope to observe the inside mature adipocyte of standing groove 2.
In other embodiments, the through slots 5 provided on the side wall of the base 4 may be provided in other numbers or other shapes, and the through slots 5 are opened lower than the ceiling member 1, so as to prevent mature fat cells below the ceiling member 1 from flowing out of the base 4 through the through slots 5.
According to the ceiling device for the wall-attached culture of floating cells, the ceiling plate 1 and the base 4 are arranged, the ceiling plate 1 is fixedly connected to the upper side of the base 4 in a bonding mode and the like, a plurality of placing grooves 2 are formed in one side, close to the base 4, of the ceiling plate 1, when fat cells are observed, the inside of the base 4 is inserted into the base 4 through square through grooves 5 in the periphery of the base 4 through the pipettor 7, the lower portion of the ceiling plate 1 is placed, fresh separated mature fat cells in the pipettor 7 are injected into a culture solution below the ceiling plate 1, the mature fat cells float close to the ceiling plate 1, and occupy the inside of the placing grooves 2 in the ceiling plate 1 according to different cell diameter distribution, so that limit is carried out on the mature fat cells, observation is facilitated on the mature fat cells in a body, the problem that the mature fat cells are difficult to observe due to floating on the liquid surface is solved, direct cognition on the fat cells and fat cells are facilitated, a large amount of preparation of autologous fat dedifferentiated stem cells is facilitated, and the method is used for autologous cell therapy.
In other embodiments, as shown in fig. 3, the base 4 may also be configured as a conventional cell culture flask, on which the ceiling plate 1 is connected in a sealing manner, and the placement groove 2 of the ceiling plate 1 is disposed inside the base 4, so as to form a new fat cell culture flask, the whole culture flask is required to be filled with cell suspension and culture solution, and the sealing cover 6 is sealed.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the utility model.
Claims (9)
1. A ceiling apparatus for use in the adherent culture of floating cells, comprising:
a plurality of placing grooves are formed in one side of the ceiling plate, and floating cells are placed in the placing grooves;
the base is internally provided with an upward opening placing cavity, and the ceiling plate is arranged on the opening side of the placing cavity to cover the opening of the placing cavity;
the placing cavity is internally suitable for placing culture solution and floating cells, and the placing groove is formed in one side of the ceiling plate facing the placing cavity and is in contact with the culture solution, so that the floating cells in the placing cavity enter the placing groove.
2. The ceiling apparatus for use in the adherent culture of floating cells of claim 1, wherein said placement groove is a quarter-spherical groove.
3. The ceiling apparatus for use in the adherent culture of floating cells according to claim 2, wherein adjacent two of said placement tank side walls are connected to each other.
4. The ceiling apparatus for use in the adherent culture of floating cells of claim 2, wherein the spherical recess has a diameter of 180-220 microns.
5. The ceiling device for adherent culture of floating cells according to any one of claims 1-4, wherein the base is adapted to be placed in an external culture dish and wherein a plurality of through slots are provided in the placement cavity in the base for allowing external culture fluid and floating cells to enter the placement cavity.
6. The ceiling apparatus for use in the adherent culture of floating cells of claim 5 wherein said through slot opening is lower than said ceiling plate.
7. The ceiling apparatus for use in the adherent culture of floating cells of claim 6, wherein the base bottom is provided as an opening.
8. The ceiling apparatus for use in the adherent culture of floating cells of claim 7, wherein the base is less than 1cm in height.
9. The ceiling device for use in the adherent culture of floating cells according to any one of claims 1 to 4, wherein an opening is provided in the placement chamber in the base, and a sealing cover is provided on the opening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320419147.9U CN219621189U (en) | 2023-03-07 | 2023-03-07 | Ceiling device for wall-attached culture of floating cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320419147.9U CN219621189U (en) | 2023-03-07 | 2023-03-07 | Ceiling device for wall-attached culture of floating cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219621189U true CN219621189U (en) | 2023-09-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320419147.9U Active CN219621189U (en) | 2023-03-07 | 2023-03-07 | Ceiling device for wall-attached culture of floating cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219621189U (en) |
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2023
- 2023-03-07 CN CN202320419147.9U patent/CN219621189U/en active Active
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