CN219260049U

CN219260049U - Microfluidic chip

Info

Publication number: CN219260049U
Application number: CN202320127402.2U
Authority: CN
Inventors: 肖同杰; 宋彦廷
Original assignee: Hainan University
Current assignee: Hainan University
Priority date: 2023-01-13
Filing date: 2023-01-13
Publication date: 2023-06-27
Anticipated expiration: 2033-01-13

Abstract

The utility model provides a microfluidic chip, which comprises a chip part, wherein the chip part comprises an upper layer channel and a lower layer channel, the upper layer channel and the lower layer channel are arranged in a crisscross manner and are separated by a microporous membrane, an elliptic cylindrical upper layer culture cavity is arranged in the middle of the upper layer channel, a cylindrical lower layer culture cavity is arranged in the middle of the lower layer channel, the center of the lower layer culture cavity and the center of the upper layer culture cavity are positioned on the same central line, and the lower layer culture cavity is internally tangent to the upper layer culture cavity in the extending direction. Compared with the traditional Transwell model, the microfluidic chip can provide a physiological environment similar to that in vivo for cells, tissues and the like, and can greatly save animal resources and research cost. Meanwhile, the device has the characteristics of simple configuration, low manufacturing cost, simple operation, high efficiency and easy cell and tissue growth.

Description

Microfluidic chip

Technical Field

The utility model belongs to the field of microfluidic chips and the fields of medicine, pharmacy and biological detection, and relates to a microfluidic chip.

Background

The microfluidic technology is a technical means for accurately manipulating micro-fluid in a micro-level microtube, and can be used for experimental research of chemistry, biology and the like in a chip with a few square centimeters.

It is well known that in vivo systems can provide a natural environment for research, but limit understanding of normal physiological processes and mechanisms of pathological states, and do not allow quantitative research or high throughput analysis in vivo. The traditional Transwell model ignores shear forces generated by blood flow as a static cultured in vitro model and does not simulate physiological structures well.

In recent years, the technology of microfluidic chips is rapidly developed, and the establishment of various in-vitro models greatly improves the efficiency of related researches. However, most microfluidic chips are complex in design, high in manufacturing cost, and difficult to operate and have not been widely used.

Disclosure of Invention

The present application provides a microfluidic chip for solving the above problems. Compared with the traditional Transwell model, the microfluidic chip can provide a physiological environment similar to that in vivo for cells, tissues and the like, and can greatly save animal resources and research cost. Meanwhile, the device has the characteristics of simple configuration, low manufacturing cost, simple operation, high efficiency and easy cell and tissue growth.

In order to achieve the purpose of the application, the technical scheme adopted by the application is as follows:

the microfluidic chip comprises a chip part, wherein the chip part comprises an upper layer channel and a lower layer channel, the upper layer channel and the lower layer channel are arranged in a crisscross manner and are separated by a microporous membrane, an elliptical cylindrical upper layer culture cavity is arranged in the middle of the upper layer channel, a cylindrical lower layer culture cavity is arranged in the middle of the lower layer channel, the center of the lower layer culture cavity and the center of the upper layer culture cavity are positioned on the same central line, and the lower layer culture cavity is inscribed in the upper layer culture cavity in the extending direction.

Preferably, the upper layer culture cavity and the lower layer culture cavity are both connected with a fluid channel, and the fluid channel and the upper layer culture cavity and the lower layer culture cavity are both provided with width differences.

More preferably, the ratio of the width of the fluid channel to the width of the upper culture chamber and the lower culture chamber is in the range of 1:2-1:10.

more preferably, the fluid channel is provided with an inlet and an outlet.

Preferably, the microfluidic chip further comprises a slide for supporting the chip portion.

Preferably, the material of the chip part is Polydimethylsiloxane (PDMS).

Preferably, the microporous membrane has a thickness of 10 μm and a membrane pore size of 0.4 to 8.0 μm.

More preferably, the material of the microporous membrane is selected from Polycarbonate (PC) or polyethylene terephthalate (PET).

Preferably, the length of the upper layer channel and the lower layer channel is 0.5-5cm, and the height of the upper layer channel and the lower layer channel is 0.1-0.2mm.

More preferably, the oval cylindrical upper culture chamber is 1-5mm long and 0.2-2mm wide, and the cylindrical lower culture chamber is 0.2-2mm in diameter.

The beneficial effects of this application lie in:

the unique cell culture cavity configuration design, the lower layer culture cavity is cylindrical, and the culture cavity is inscribed in the upper layer culture cavity from the top view, so that the contact area of the upper layer channel and the lower layer channel is defined, and the calculation of common parameters in a cell or tissue model, such as apparent permeability coefficient and the like, is facilitated. Meanwhile, the chip culture cavity is designed to be arc-shaped, so that the problem that the development of cells or tissues is affected due to the formation of intractable bubbles can be effectively avoided. The difference between the width of the fluid channel and the width of the culture cavity can facilitate the enrichment and uniform distribution of cells or tissues. Provides a microfluidic chip with convenient operation and high efficiency for constructing in vitro physiological models, such as barrier models and the like.

Drawings

Fig. 1 is an overall top view of a microfluidic chip of the present application.

Fig. 2 is a top view of an upper channel of a microfluidic chip according to the present application.

Fig. 3 is a top view of a lower channel of a microfluidic chip according to the present application.

In the figure:

1-glass slide, 2-chip part, 3-inlet and outlet, 4-fluid channel, 5-upper culture cavity and 6-lower culture cavity.

Detailed Description

The present utility model will be described in detail with reference to the following specific examples, which will assist those skilled in the art in further understanding the present utility model, but are not intended to limit the present utility model in any way. It should be noted that several variations or modifications can be made to the device without departing from the inventive concept. These are all within the scope of the present utility model.

In the description of the present utility model, it should be noted that the terms "disposed," "connected," and the like should be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; may be directly connected, indirectly connected through an intermediate medium, or may be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

In addition, if not specifically described, the components adopted in the following embodiments are all existing components, and their corresponding connection modes can also be implemented by conventional technical means, which will not be described in detail in this application.

Examples

As shown in fig. 1 to 3, the microfluidic chip provided by the utility model comprises a glass slide 1 and a chip part 2, wherein the glass slide 1 has the main function of increasing the physical strength of the chip part 2, playing a supporting role, and simultaneously being convenient for fixation and observation under a microscope. The main material of the chip part 2 is Polydimethylsiloxane (PDMS), which has good air permeability and light transmittance, and is easy for growth of cells, tissues, etc. and observation under a mirror.

The chip part 2 comprises an upper layer channel and a lower layer channel which are arranged in a crisscross manner and are separated by a microporous membrane with the thickness of 10 mu m, wherein the microporous membrane material can be Polycarbonate (PC) or polyethylene terephthalate (PET), and the pore diameter of the membrane is 0.4-8.0 mu m. The length of the upper and lower layers of channels is 0.5-5cm, and the height is 0.1-0.2mm.

An elliptic cylindrical upper layer culture cavity 5 is arranged in the middle of the upper layer channel, the length of the elliptic cylindrical upper layer culture cavity is 1-5mm, the width of the elliptic cylindrical upper layer culture cavity is 0.2-2mm, a cylindrical lower layer culture cavity 6 is arranged in the middle of the lower layer channel, the diameter of the lower layer culture cavity is 0.2-2mm, the center of the lower layer culture cavity 6 and the center of the upper layer culture cavity 5 are located on the same central line, and the lower layer culture cavity 6 is inscribed in the upper layer culture cavity 5 in the extending direction. The upper layer culture cavity 5 and the lower layer culture cavity 6 are both connected with a fluid channel 4, the fluid channel 4 and the upper layer culture cavity 5 and the lower layer culture cavity 6 are both provided with width differences, and the width proportion range is 1:2-1:10, the design can generate fluid velocity difference in the inoculation process, and when the cell or tissue suspension passes through the culture cavity, the flow velocity is slowed down, so that the cells or tissues are conveniently enriched in the culture cavity and uniformly distributed.

The fluid channel 4 is provided with an inlet and an outlet 3, and the diameter of the inlet and the outlet 3 is 0.5-2.5mm.

The microfluidic chip can be suitable for the construction of single culture, contact co-culture and non-contact co-culture models of cells or tissues. Taking a contact co-culture model as an example, selecting a sterile injector with proper specification and a special pipe, sucking a proper amount of solution such as human fibronectin or matrigel according to experimental requirements, injecting the solution into a chip through a microfluidic pump, and carrying out surface treatment on a culture cavity so as to enable cells or tissues to be easily attached. Then, incubating in a carbon dioxide incubator at 37 ℃, taking out, cleaning the channel with a complete culture medium, injecting a prepared lower cell or tissue suspension into the lower channel until the culture cavity is full, and placing the chip upside down into the incubator to adhere cells or tissues; and (3) inoculating upper cells or tissues into the upper culture cavity, connecting a microfluidic pump to the chip after the upper cells or tissues are attached, continuously injecting a complete culture medium into the chip at a proper flow rate, simulating an in-vivo fluid environment, and dynamically culturing the cells or tissues in the chip until a model is built.

It should be understood that the foregoing examples of the present utility model are merely illustrative of the present utility model and not limiting of the embodiments of the present utility model, and that various other changes and modifications can be made by those skilled in the art based on the above description, and it is not intended to be exhaustive of all the embodiments of the present utility model, and all obvious changes and modifications that come within the scope of the utility model are defined by the following claims.

Claims

1. The microfluidic chip is characterized by comprising a chip part, wherein the chip part comprises an upper layer channel and a lower layer channel, the upper layer channel and the lower layer channel are arranged in a crisscross manner and are separated by a microporous membrane, an elliptic cylindrical upper layer culture cavity is arranged in the middle of the upper layer channel, a cylindrical lower layer culture cavity is arranged in the middle of the lower layer channel, the center of the lower layer culture cavity and the center of the upper layer culture cavity are positioned on the same central line, and the lower layer culture cavity is internally tangent to the upper layer culture cavity in the extending direction.

2. The microfluidic chip according to claim 1, wherein the upper culture chamber and the lower culture chamber are connected with fluid channels, and the fluid channels have a width difference from the upper culture chamber and the lower culture chamber.

3. The microfluidic chip according to claim 2, wherein the ratio of the width of the fluid channel to the width of the upper and lower culture chambers ranges from 1:2-1:10.

4. a microfluidic chip according to claim 2 or 3, wherein the fluid channel is provided with an inlet and an outlet.

5. The microfluidic chip of claim 1, further comprising a slide for supporting the chip portion.

6. A microfluidic chip according to claim 1, wherein the material of the chip portion is polydimethylsiloxane PDMS.

7. The microfluidic chip according to claim 1, wherein the microporous membrane has a thickness of 10 μm and a membrane pore size of 0.4 to 8.0 μm.

8. A microfluidic chip according to claim 1 or 7, wherein the material of the microporous membrane is selected from polycarbonate PC or polyethylene terephthalate PET.

9. The microfluidic chip according to claim 1, wherein the upper and lower channels are each 0.5-5cm in length and 0.1-0.2mm in height.

10. The microfluidic chip according to claim 9, wherein the upper culture chamber is 1-5mm long and 0.2-2mm wide, and the lower culture chamber is 0.2-2mm in diameter.