CN218879874U

CN218879874U - Sperm optimizing device

Info

Publication number: CN218879874U
Application number: CN202320521451.4U
Authority: CN
Inventors: 陈春晓; 任潇宇; 毕方成; 孟荣华; 王双; 毕胜成
Original assignee: Taipingyang Kangtai Scientific Apparatus Jinan Co ltd
Current assignee: Taipingyang Kangtai Scientific Apparatus Jinan Co ltd
Priority date: 2023-03-17
Filing date: 2023-03-17
Publication date: 2023-04-18
Anticipated expiration: 2033-03-17

Abstract

A sperm optimizing device relates to the technical field of auxiliary reproduction medical instruments and is used for optimizing sperms with better vitality. The device comprises a preferable dish and a dish cover, wherein a storage groove and a preferable groove are arranged in the upper surface of the preferable dish, a plurality of independent microfluidic channels are arranged between the storage groove and the preferable groove, and transition grooves are arranged on the microfluidic channels; the dish cover is located above the preferred dish and used for sealing the preferred dish, and the dish cover is detachably connected with the preferred dish. The utility model stores the semen through the arrangement of the storage tank, and provides a channel for the movement of the semen through the arrangement of the microfluidic channel; the transition groove is arranged to provide a temporary end point for the sperms, the sperms with general activity can stay in the transition groove, the sperms with stronger activity can continue to move to the optimal groove along the microfluidic channel, and the sperms with the best activity are preferentially selected.

Description

Sperm optimizing device

Technical Field

The utility model relates to the technical field of auxiliary reproduction medical equipment, in particular to a sperm optimizing device.

Background

At present, two conventional methods for optimizing semen are provided, namely an upstream method and a density gradient centrifugation method. The upstream method is suitable for semen specimen with normal semen indexes in semen routine examination, and adopts the principle that the motile sperm has the motility and can swim upwards from the bottom of the test tube and swim into the liquid level layer of the culture solution. While the motile, immotile, agglutinated and other cellular components remain at the bottom of the tube. By sucking the culture solution on the upper layer, the sperms with better activity can be collected, and the aim of optimizing the sperms is fulfilled. The upstream method has the defects that the upward swimming stroke of the sperms against the gravity is long, and the sperm recovery rate is low. (2) The density gradient centrifugation method is suitable for semen samples with normal semen and little weak semen. Preparing gradient liquid into gradient layers with different concentrations from top to bottom and from low to high, and placing the liquefied semen on the uppermost layer. After centrifugation, the sperm enter different concentration layers according to different sperm quality and different specific gravities of other components. Most of the sperms with good vitality and normal shape enter the high-density layer, and the sperms with better quality can be separated out by matching with centrifugation. The density gradient centrifugation method has the defect that the semen sample needs to be transferred and centrifuged for multiple times, and has certain damage to sperms.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a preferred device of sperm of simple structure for the realization is to the preferred of the better sperm of vitality.

The utility model provides a technical scheme that its technical problem adopted is: the sperm optimizing device comprises an optimizing vessel and a vessel cover, wherein a storage groove and an optimizing groove are arranged in the upper surface of the optimizing vessel, a plurality of mutually independent microfluidic channels are arranged between the storage groove and the optimizing groove, and transition grooves are arranged on the microfluidic channels; the ware lid is located preferred ware top and carries out the closing cap to preferred ware, just the ware lid can be dismantled with preferred ware and be connected.

Further, the lower part of the dish cover is in threaded connection with the upper part of the optimized dish.

Further, the upper part of the preferable dish is provided with a groove, and the lower part of the dish cover is inserted into the groove.

Further, the depth of the reservoir is greater than the depth of the preferred tank.

Further, the volume of the reservoir tank is larger than the volume of the preferred tank.

Further, the transition groove has a spherical inner wall, and is located in the middle of the microfluidic channel.

Further, the microfluidic channel is arc-shaped.

Further, the preferred dish is of circular or oval configuration.

Furthermore, the microfluidic channels are provided with a plurality of groups, and the two microfluidic channels which are arranged in groups are symmetrically arranged by taking the connecting line of the optimized groove and the storage groove as a symmetrical line.

The water tank is further included, the bottom of the optimized dish is hollow to form a bottom groove, the middle lower part of the optimized dish is placed in the water tank, and warm water is filled in the water tank.

The utility model has the advantages that: the utility model stores the semen through the arrangement of the storage tank, and provides a channel for the movement of the semen through the arrangement of the microfluidic channel; the transition groove is arranged to provide a temporary end point for the sperms, the sperms with general activity can stay in the transition groove, the sperms with stronger activity can continue to move to the optimal groove along the microfluidic channel, and the sperms with the best activity are preferentially selected.

Drawings

Fig. 1 is a front view of a first embodiment of the present invention;

FIG. 2 is one of three-dimensional views of a preferred dish according to the first embodiment;

FIG. 3 is a second three-dimensional view of a preferred dish according to the first embodiment;

FIG. 4 is a top view of the preferred dish of the first embodiment;

FIG. 5 isbase:Sub>A sectional view taken along line A-A of FIG. 4;

FIG. 6 is a sectional view taken along line B-B of FIG. 4;

fig. 7 is a cross-sectional view of a first embodiment of the present invention;

FIG. 8 is a top view of a preferred vessel according to a second embodiment of the present invention;

FIG. 9 is a sectional view of a preferred vessel in accordance with a third embodiment of the present invention;

in the figure: 1 is preferably a dish, 11 is preferably a groove, 12 is a storage groove, 13 is a microfluidic channel, 14 is a transition groove, 15 is a bottom groove, 16 is a groove, 2 is a dish cover, 3 is a water groove, and 4 is warm water.

Detailed Description

As shown in fig. 1 to 9, the present invention comprises a preferred dish 1 and a dish cover 2, and the present invention will be described in detail with reference to the accompanying drawings.

The first embodiment is as follows:

as shown in fig. 1 and 7, the sperm optimizing device comprises an optimizing dish 1 and a dish cover 2, wherein the upper part of the optimizing dish 1 is provided with a groove 16, and the lower part of the dish cover 2 is inserted into the groove 16 to realize the sealing of the optimizing dish 1. The lower part of the dish cover 2 can also be provided with an external thread, the inner wall of the groove 16 is provided with an internal thread, and then the lower part of the dish cover 2 is in threaded connection with the upper part of the optimized dish 1. As shown in FIG. 3, it is preferable that the bottom of the dish 1 is hollowed to form a bottom groove 15 to reduce the overall weight.

As shown in fig. 2 and 4, the upper surface of the preferred dish 1 has a storage groove 12 and a preferred groove 11, a plurality of independent microfluidic channels 13 are arranged between the storage groove 12 and the preferred groove 11, one end of each microfluidic channel 13 is communicated with the storage groove 12, and the other end of each microfluidic channel 13 is communicated with the preferred groove 11. The depth of the reservoir 12 is greater than the depth of the preferred reservoir 11, and the volume of the reservoir 12 may be 2-4ml, and the volume of the preferred reservoir 11 may be 1-2ml. The preferred dish 1 is of circular configuration and as shown in fig. 6, the reservoir 12 and the preferred well 11 are located on the same diameter of the preferred dish 1 to maximize the distance between the reservoir 12 and the preferred well 11. The width and depth of the microfluidic channel 13 have various dimensions, the width of the microfluidic channel 13 may be 5mm, 1mm or 500um, and the depth of the microfluidic channel 13 may be 4mm, 1mm or 500um. As shown in fig. 5, the microfluidic channel 13 has a transition groove 14 thereon, the transition groove 14 has a spherical inner wall, and the diameter of the transition groove 14 has various size choices, such as 5mm, 4mm, 1mm or 500um. The transition groove 14 is positioned in the middle of the microfluidic channel 13 to divide the microfluidic channel 13 into two sections, and the two sections of the microfluidic channel 13 are communicated through the transition groove 14. The shape of the microfluidic channel 13 may be curved, circular arc or zigzag, and the shape of the microfluidic channel 13 is preferably circular arc. The microfluidic channels 13 have a plurality of groups, and two microfluidic channels 13 arranged in a group are symmetrically arranged by taking a connecting line of the preferred groove 11 and the storage groove 12 as a symmetrical line.

The following describes the method of use of the present invention:

(1) Putting the liquefied semen and the culture solution into a storage tank 12, and covering a dish cover;

(2) And (3) standing the preferable dish for a period of time, wherein the sperms enter the microfluidic channel from the storage groove by virtue of self-movement capacity in the process, and the sperms with good movement power move to the preferable groove 11 through the microfluidic channel. After a certain period of time, the sperm in the preferred well 11 is aspirated and transferred to fertilization.

Example two:

as shown in fig. 8, the difference between the second embodiment and the first embodiment is that the shape of the dish 1 is different, that is, the dish 1 in the second embodiment has an elliptical structure, and the dish lid 2 engaged with the elliptical structure. Example two is described in detail below

The sperm optimizing device in the second embodiment comprises an optimizing dish 1 and a dish cover 2, wherein the optimizing dish 1 is of an oval structure, the upper part of the optimizing dish 1 is provided with an oval groove 16, the dish cover 2 is also oval, and the lower part of the dish cover 2 is inserted into the groove 16 to realize the sealing of the optimizing dish 1. Preferably, the bottom of the dish 1 is hollowed to form a bottom groove 15 to reduce the overall weight.

The upper surface of the optimized dish 1 is provided with a storage groove 12 and an optimized groove 11, a plurality of independent micro-fluidic channels 13 are arranged between the storage groove 12 and the optimized groove 11, one end of each micro-fluidic channel 13 is communicated with the storage groove 12, and the other end of each micro-fluidic channel 13 is communicated with the optimized groove 11. The depth of the reservoir 12 is greater than the depth of the preferred tank 11, and the volume of the reservoir 12 may be 2-4ml, and the volume of the preferred tank 11 may be 1-2ml.

The reservoir 12 and the preferred well 11 are located on the line connecting the two foci of the ellipse in which the preferred dish 1 is located, so that the distance between the reservoir 12 and the preferred well 11 is maximized. The width and depth of the microfluidic channel 13 have various dimensions, the width of the microfluidic channel 13 may be 5mm, 1mm or 500um, and the depth of the microfluidic channel 13 may be 4mm, 1mm or 500um. The microfluidic channel 13 has a transition groove 14, the transition groove 14 has a spherical inner wall, and the diameter of the transition groove 14 has various size choices, such as 5mm, 4mm, 1mm or 500um. The transition groove 14 is positioned in the middle of the microfluidic channel 13 to divide the microfluidic channel 13 into two sections, and the two sections of the microfluidic channel 13 are communicated through the transition groove 14. The shape of the microfluidic channel 13 may be curved, circular arc or zigzag, and the shape of the microfluidic channel 13 is preferably circular arc. The microfluidic channels 13 have a plurality of groups, and two microfluidic channels 13 arranged in a group are symmetrically arranged by taking a connecting line of the preferred groove 11 and the storage groove 12 as a symmetrical line.

Compared with the preferred dish 1 of the first embodiment, the preferred dish 1 of the second embodiment has the advantages that the distance between the preferred groove 11 and the storage groove 12 and the length of the microfluidic channel are relatively larger, so that sperm with stronger motility can be screened out, and the preferred quality is ensured. The sperm cell preference apparatus of example two is used in the same manner as the sperm cell preference apparatus of example one.

EXAMPLE III

As shown in fig. 9, the third embodiment is different from the first embodiment in that a water tank 3 is further provided. Preferably, the lower middle portion of the dish 1 is placed in a water tank 3, and the water tank 3 is filled with warm water 4, such as warm water at 37 ℃. The setting of basin 3 for splendid attire warm water when placing preferred groove 1 in basin 3, can keep warm to hold up tank 12 and preferred groove 11, and then makes the sperm be in under being equated with human body temperature's temperature environment, and then guarantees the activity of sperm.

The utility model stores the semen through the arrangement of the storage tank, and provides a channel for the movement of the semen through the arrangement of the microfluidic channel; the transition groove is arranged to provide a temporary end point for the sperms, the sperms with general activity can stay in the transition groove, the sperms with stronger activity can continue to move to the optimal groove along the microfluidic channel, and the sperms with the best activity are preferentially selected. The arrangement of the water tank can provide a temperature environment which is equal to the body temperature of a human body for the storage tank and the optimal tank, and further the activity of sperms is ensured.

Claims

1. The sperm optimizing device is characterized by comprising an optimizing dish and a dish cover, wherein a storage groove and an optimizing groove are arranged in the upper surface of the optimizing dish, a plurality of mutually independent microfluidic channels are arranged between the storage groove and the optimizing groove, and transition grooves are arranged on the microfluidic channels; the ware lid is located preferred ware top and carries out the closing cap to preferred ware, just the ware lid can be dismantled with preferred ware and be connected.

2. The sperm cell preference device of claim 1, wherein the capsule lower portion is threadably coupled to the preferred capsule upper portion.

3. The sperm cell preference device of claim 1, wherein the preference dish has a recess in an upper portion thereof and the lower portion of the dish lid is inserted into the recess.

4. The sperm cell preference device of claim 1, wherein the reservoir has a depth greater than the depth of the preference reservoir.

5. The sperm cell preference device of claim 4, wherein the volume of the reservoir is greater than the volume of the preference reservoir.

6. The sperm cell preference device of claim 1, wherein the transition channel has a spherical inner wall, the transition channel being located in the middle of the microfluidic channel.

7. The sperm cell preference device of claim 1, wherein the microfluidic channel is circular.

8. The sperm cell preference device of claim 1, wherein the preference dish is of a circular or oval configuration.

9. The sperm optimizing apparatus of claim 1 wherein the microfluidic channel has a plurality of sets, two microfluidic channels arranged in a set being symmetrically arranged with respect to a line connecting the optimization reservoir and the reservoir.

10. The sperm optimizing apparatus according to claim 1, further comprising a water tank, wherein the bottom of said optimized dish is hollow to form a bottom tank, the middle lower part of said optimized dish is placed in the water tank, and the water tank is filled with warm water.