CN217722510U - Cell cryopreserving pipe group - Google Patents

Cell cryopreserving pipe group Download PDF

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Publication number
CN217722510U
CN217722510U CN202123297971.1U CN202123297971U CN217722510U CN 217722510 U CN217722510 U CN 217722510U CN 202123297971 U CN202123297971 U CN 202123297971U CN 217722510 U CN217722510 U CN 217722510U
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China
Prior art keywords
test tube
tube
cell
pipe
sealing ring
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CN202123297971.1U
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Chinese (zh)
Inventor
李栋
刘抗
张天友
邓恩杰
卢瑞安
戴晓宇
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Chongqing Boerfei Cell Biotechnology Co ltd
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Chongqing Boerfei Cell Biotechnology Co ltd
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Priority to CN202123297971.1U priority Critical patent/CN217722510U/en
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Abstract

The utility model provides a cell freezing tube group, in particular to the field of biological experiment equipment, which comprises a tube frame and a plurality of test tubes, wherein the tube frame comprises an upper floating plate, the upper floating plate is provided with a plurality of tube holes for placing the test tubes, the top parts of the test tubes are provided with tube caps, the outer diameters of the tube caps are larger than the outer diameters of the tube holes, the bottoms of the test tubes are internally provided with inner channels, and the inner channels extend from the bottoms of the test tubes to the tops of the test tubes and penetrate out from one sides of the test tubes; this cell cryopreserved nest of tubes can be with the test tube card on last kickboard, floats the kickboard in liquid nitrogen or warm water as the case may be, and the test tube sinks in liquid this moment, and liquid flows in and is full of the interior way from the interior way, and liquid nitrogen or warm water homoenergetic fully contact the test tube for inside cell evenly freezes or evenly unfreezes, has avoided freezing the cell damage that intraductal cell that deposits because of the temperature is inhomogeneous leads to, improves the accuracy of experiment.

Description

Cell cryopreserving pipe group
Technical Field
The utility model relates to a biological assay equipment field especially relates to a cell cryopreserving nest of tubes.
Background
Cells need to be frozen after large-scale culture so as to ensure that the number of the cells can meet the subsequent use requirement. However, in the process of cell freezing and recovery, the cells are often damaged due to uneven temperature distribution inside the freezing tube, and the activity of the cells is directly affected. The casing of freezing the pipe that deposits commonly used at present is mostly hollow cylinder, freezes to deposit the pipe and only surface energy contact liquid nitrogen or 37 ℃'s warm water, and inside freezing deposit liquid can only be through heat transfer cooling gradually or rewarming, often lies in the regional freezing deposit liquid temperature of middle part and can not reach freezing deposit or rewarming requirement.
SUMMERY OF THE UTILITY MODEL
To exist not enough among the prior art, the utility model provides a cell cryopreserved nest of tubes to solve among the prior art cryopreserved inside temperature distribution uneven, at cryopreserved and the technical problem that the recovery in-process is fragile and injures the cell.
According to the utility model discloses an embodiment, a cell cryopreserving nest of tubes, including pipe support and a plurality of test tube, the pipe support includes the kickboard, it places to have seted up a plurality of places on the kickboard the tube hole of test tube, the top of test tube is equipped with the pipe cap, the pipe cap external diameter is greater than the tube hole external diameter, the bottom of test tube inwards is equipped with the interior way, the interior way be for set up in independent passageway in the test tube, the interior way is followed the test tube bottom to the test tube top extends and follows test tube one side is worn out.
Contrast prior art, the utility model discloses following beneficial effect has:
this cell freezes deposits nest of tubes can with the test-tube card on last kickboard, floats the kickboard in liquid nitrogen or warm water as the case requires, and the test tube sinks into liquid this moment, and liquid flows in and is full of interior way from the interior way, and liquid nitrogen or warm water homoenergetic fully contact test tube for inside cell evenly freezes or evenly unfreezes, has avoided freezing the cell damage that intraductal cell that deposits leads to because of the temperature is inhomogeneous, improves the accuracy of experiment.
Further, the tube cap includes plectane, interior seal ring and outer seal ring, interior seal ring with outer seal ring sets up the bottom of plectane, interior seal ring is located outer seal ring is inboard, the top end opening clamp of test tube is established interior seal ring with between the outer seal ring.
Furthermore, a pipe handle convenient for carrying is fixedly arranged on the top surface of the circular plate.
Further, the pipe support still includes the diving board, a plurality of support columns have set firmly on the diving board, the top of support column has set firmly the brace table, the upper floating plate place in on the brace table.
Furthermore, a plurality of tube supports are fixedly arranged on the submergence plate, the length of each tube support is longer than the height of the inner channel in the test tube, and the test tube is inserted into the tube hole and placed on the tube support.
Furthermore, a sliding column is further arranged at the top end of the supporting table, a sliding hole is formed in the upper floating plate, the sliding column is slidably arranged in the sliding hole, and a limiting block is fixedly arranged at the top end of the sliding column.
Furthermore, the support columns are respectively positioned on two sides of the upper floating plate, the support columns on two sides are respectively and fixedly connected with a carrying handle, and the carrying handles are higher than the upper floating plate.
Drawings
FIG. 1 is a schematic structural view of a cell freezing tube set according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of a pipe rack according to an embodiment of the present invention;
FIG. 3 is a cross-sectional view of the embodiment of the present invention at the location of the support post;
FIG. 4 is a cross-sectional view of a test tube and a cap according to an embodiment of the present invention;
in the above drawings: 1. a pipe frame; 11. an upper floating plate; 111. a tube hole; 112. a slide hole; 12. submerging the plate; 13. a support column; 14. a support table; 15. pipe bracing; 16. a traveler; 17. a limiting block; 18. a carrying handle; 2. a test tube; 21. an inner channel; 3. a pipe cap; 31. a circular plate; 32. an inner seal ring; 33. an outer seal ring; 34. and (4) a pipe handle.
Detailed Description
The technical solution of the present invention is further explained with reference to the drawings and the embodiments.
The embodiment of the utility model provides a cell freezing tube group, as shown in figure 1 and figure 2, including pipe support 1 and a plurality of test tubes 2, twenty test tubes 2 can be placed to every pipe support 1, pipe support 1 includes upper float plate 11, upper float plate 11 material selects the plastics material that can float in liquid nitrogen and surface of water, twenty pipe holes 111 of placing test tubes 2 are seted up on upper float plate 11, pipe hole 111 is four by five arrays, as shown in figure 4, the top of test tube 2 is equipped with pipe cap 3, pipe cap 3 external diameter is greater than pipe hole 111 external diameter, pipe cap 3 includes plectane 31, inner seal ring 32 and outer seal ring 33, the external diameter of plectane 31 is greater than the external diameter of pipe hole 111, the external diameter of outer seal ring 33 equals the diameter of pipe hole 111, inner seal ring 32 with outer seal ring 33 sets up the bottom of plectane 31, inner seal ring 32 is located outer seal ring 33 inboard, the top end opening of the test tube 2 is clamped between the inner sealing ring 32 and the outer sealing ring 33, the top surface of the circular plate 31 is further fixedly provided with a tube handle 34 convenient for carrying, the whole tube cap 3 is made of rubber, the test tube 2 can be stored in a sealing manner, when the test tube 2 is placed on the tube frame 1, the test tube 2 is positioned below the upper floating plate 11, the tube cap 3 is clamped in the tube hole 111, as shown in fig. 4, the bottom of the test tube 2 is provided with an inner channel 21 inwards, the inner channel 21 is an independent channel arranged in the test tube 2, the inner channel 21 extends from the bottom of the test tube 2 to the top of the test tube 2 and penetrates out from one side of the test tube 2, the structure can clamp the test tube 2 on the upper floating plate 11, the upper floating plate 11 is floated in liquid nitrogen or warm water according to circumstances, at this time, the test tube 2 sinks into the liquid, the liquid flows into and fills the inner channel 21, the outer wall of the test tube 2 is surrounded by the liquid all the inner channel 21, liquid nitrogen or warm water homoenergetic fully contact test tube 2 for inside cell evenly freezes or evenly unfreezes, has avoided freezing the cell damage that the intraductal cell of depositing leads to because of the temperature is inhomogeneous, improves the accuracy of experiment.
In a further scheme, as shown in fig. 2 and 3, the pipe rack 1 further comprises a submergence plate 12, the submergence plate 12 is made of metal capable of sinking in liquid nitrogen and warm water, four support columns 13 are fixedly arranged on the submergence plate 12, a support table 14 is fixedly arranged at the top ends of the support columns 13, and the upper floating plate 11 is placed on the support table 14; a plurality of pipe supports 15 are fixedly arranged on the submergence plate 12, the length of each pipe support 15 is longer than the height of the inner channel 21 in the test tube 2, and the test tube 2 is inserted into the pipe hole 111 and placed on the pipe supports 15; the top end of the support table 14 is further provided with a sliding column 16, the upper floating plate 11 is provided with a sliding hole 112, the sliding column 16 is slidably arranged in the sliding hole 112, and the top end of the sliding column 16 is fixedly provided with a limit block 17; four two liang lieing in of support column 13 go up the both sides of kickboard 11, both sides support column 13 is fixedly connected with handheld handle 18 respectively, the height of handheld handle 18 is higher than go up kickboard 11, dive board 12 is convenient for vertically place test tube 2, when kickboard 11 arranges supporting bench 14 in, the plane can directly be arranged in to pipe support 1, test tube 2 penetrates the back from tube hole 111, pipe brace 15 penetrates from test tube 2 bottom, and support the turn of lane 21 at test tube 2, test tube 2 is fixed on pipe support 1 by steady this moment, when needs place test tube 2 in liquid, both hands mention handheld handle 18, arrange pipe support 1 in liquid, dive board 12 shifts down and is limited in last kickboard 11 below by stopper 17, come up board 11 and float in the surface of water, pipe brace 15 kicks open test tube 2, each surface of test tube 2 this moment can be abundant with liquid contact, after cell in 2 evenly unfreezes or freezes, mention handheld handle 18, can once only be with 1 test tube 2 of whole test tube, this test tube structure is lifted, the external world is convenient for the test tube 2 of placing, the damage of being convenient for the test tube 2 of test tube 2, the test tube 2 that the collision of being convenient for this time prevents.
Finally, it is noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the scope of the claims of the present invention.

Claims (7)

1. A cell freezing tube group is characterized in that: including pipe support (1) and a plurality of test tube (2), pipe support (1) is including kickboard (11), go up and seted up a plurality of places on kickboard (11) tube hole (111) of test tube (2), the top of test tube (2) is equipped with cap (3), cap (3) external diameter is greater than tube hole (111) external diameter, the bottom of test tube (2) is inwards equipped with interior way (21), interior way (21) be for setting up in independent passageway in test tube (2), interior way (21) are followed test tube (2) bottom to test tube (2) top extends and follows test tube (2) one side is worn out.
2. The set of cell freezers of claim 1, wherein: the pipe cap (3) comprises a circular plate (31), an inner sealing ring (32) and an outer sealing ring (33), wherein the inner sealing ring (32) and the outer sealing ring (33) are arranged at the bottom of the circular plate (31), the inner sealing ring (32) is positioned on the inner side of the outer sealing ring (33), and the top end opening of the test tube (2) is clamped between the inner sealing ring (32) and the outer sealing ring (33).
3. The set of cell freezers of claim 2, wherein: the top surface of the circular plate (31) is also fixedly provided with a pipe handle (34) convenient for hand carrying.
4. The set of cell freezers of claim 1, wherein: pipe support (1) still includes dive board (12), a plurality of support columns (13) have set firmly on dive board (12), the top of support column (13) has set firmly brace table (14), it places to go up kickboard (11) on brace table (14).
5. The set of cell freezers of claim 4, wherein: the submergence plate (12) is fixedly provided with a plurality of pipe supports (15), the length of each pipe support (15) is higher than the height of the inner channel (21) in the test tube (2), and the test tube (2) is inserted into the pipe hole (111) and placed on the pipe support (15).
6. The set of cell freezers of claim 4, wherein: the top end of the supporting platform (14) is further provided with a sliding column (16), a sliding hole (112) is formed in the upper floating plate (11), the sliding column (16) is arranged in the sliding hole (112) in a sliding mode, and a limiting block (17) is fixedly arranged at the top end of the sliding column (16).
7. The set of cell freezers of claim 4, wherein: the supporting columns (13) are respectively positioned on two sides of the upper floating plate (11), the supporting columns (13) on the two sides are respectively and fixedly connected with a handle (18), and the height of the handle (18) is higher than that of the upper floating plate (11).
CN202123297971.1U 2021-12-24 2021-12-24 Cell cryopreserving pipe group Active CN217722510U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202123297971.1U CN217722510U (en) 2021-12-24 2021-12-24 Cell cryopreserving pipe group

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202123297971.1U CN217722510U (en) 2021-12-24 2021-12-24 Cell cryopreserving pipe group

Publications (1)

Publication Number Publication Date
CN217722510U true CN217722510U (en) 2022-11-04

Family

ID=83810826

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202123297971.1U Active CN217722510U (en) 2021-12-24 2021-12-24 Cell cryopreserving pipe group

Country Status (1)

Country Link
CN (1) CN217722510U (en)

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