CN211847940U

CN211847940U - Auxiliary sample adding device for PCR reaction tube

Info

Publication number: CN211847940U
Application number: CN202020268071.0U
Authority: CN
Inventors: 夏国庆
Original assignee: Changzhou Baidai Biotechnology Co ltd
Current assignee: Changzhou Baidai Biotechnology Co ltd
Priority date: 2020-03-06
Filing date: 2020-03-06
Publication date: 2020-11-03
Anticipated expiration: 2030-03-06

Abstract

The utility model discloses an auxiliary sample adding device for PCR reaction tubes, which belongs to the technical field of biological instruments. This supplementary application of sample device that PCR reaction was used, including end box and eight groups PCR reaction body, the quantity of every group PCR reaction body is twelve, the top fixedly connected with roof of end box, eight groups of round holes have been seted up to the upper surface of roof, the quantity of every group round hole is twelve, eight groups PCR reaction body plug-in connections respectively to the inside of eight groups of round holes, the top of end box is equipped with the lid, the inner wall of lid and the side swing joint of roof, the bottom of every group PCR reaction body all is equipped with a set of tray. The utility model discloses a set up holding in the palm piece, connecting rod, montant, circle piece and L shape pole, the condition in the application of sample in-process is difficult for observing PCR reaction tube to the place frame of having solved present common PCR reaction tube application of sample to and be not convenient for with the problem that PCR reaction tube took out from the rack.

Description

Auxiliary sample adding device for PCR reaction tube

Technical Field

The utility model belongs to the technical field of biological instrument, particularly, relate to an auxiliary application of sample device that PCR reaction was used.

Background

PCR reaction is a common experimental technique in the field of molecular biology, namely polymerase chain reaction, is a method for synthesizing specific DNA fragments in vitro enzymatically, a period is formed by high-temperature denaturation, low-temperature annealing, proper-temperature extension and other steps of reaction, and the reaction is circularly carried out, so that DNA can be rapidly amplified, and the method has the characteristics of strong specificity, high sensitivity, simplicity and convenience in operation, time saving and the like.

In the prior art, the upper surface of a common PCR reaction tube sample adding rack is generally a plane, so that the defect that the condition in the PCR reaction tube is difficult to observe in the sample adding process exists, and the PCR reaction tube is not convenient to take out from the rack.

SUMMERY OF THE UTILITY MODEL

The utility model provides an auxiliary sample adding device for PCR reaction tube, which has the advantages of being convenient for observing the conditions in the PCR reaction tube and taking out the PCR reaction tube from the placing rack in the sample adding process.

For the realization be convenient for observe the condition in the PCR reaction tube and be convenient for with the purpose that the PCR reaction tube taken out from the rack in the application of sample in-process, the utility model provides a following technical scheme: an auxiliary sample adding device for a PCR reaction tube comprises a bottom box and eight groups of PCR reaction tube bodies, wherein the number of each group of PCR reaction tube bodies is twelve, the top of the bottom box is fixedly connected with a top plate, eight groups of round holes are arranged on the upper surface of the top plate, the number of each group of round holes is twelve, eight groups of PCR reaction tube bodies are respectively inserted into the eight groups of round holes, the top of the bottom box is provided with a box cover, the inner wall of the box cover is movably connected with the side surface of the top plate, the bottom of each group of PCR reaction tube body is provided with a group of support parts, the number of the support parts is twelve, the bottom of each group of support parts is fixedly connected with a connecting rod, elongated slots are formed in the positions, corresponding to the two ends of the eight connecting rods, of the left side and the right side of the bottom box, and the left ends and the right ends of the eight connecting rods movably penetrate through the elongated slots and extend to the two sides of the bottom box.

As a preferred technical scheme of the utility model, the equal fixedly connected with fixed plate in lid side all around, the hand catching groove has been seted up in the outside of fixed plate.

As a preferred technical scheme of the utility model, every the equal fixedly connected with pipe of lower surface of connecting rod, every the equal sliding connection in inside of pipe has the montant, and connecting rod sliding connection is on the surface of montant, the both ends of montant respectively with the top and the bottom fixed connection of end box inner wall.

As an optimal technical scheme of the utility model, every the pipe quantity of connecting rod lower surface is two.

As a preferred technical scheme of the utility model, every the equal fixedly connected with dead lever of upper surface at connecting rod both ends, the top fixedly connected with circle piece of dead lever, the surface of circle piece is rotated and is connected with the rotation piece, the side fixedly connected with L shape pole of rotating the piece.

As an optimized technical proposal of the utility model, the upper surface of the support piece is sunken, and the shape of the support piece is matched with the shape of the PCR reaction tube body.

Compared with the prior art, the utility model provides an auxiliary sample adding device for PCR reaction tube possesses following beneficial effect: by arranging the supporting piece, the connecting rods, the vertical rod, the round block and the L-shaped rods, when the PCR reaction tube is added with sample, one of the eight connecting rods is lifted up, then one group of PCR reaction tubes penetrates through one group of round holes from top to bottom, the PCR reaction tubes are supported by the supporting piece, one group of PCR reaction tubes are put on the supporting piece, the sample can be added at the moment, most of the PCR reaction tubes are positioned above the top plate in the sample adding process, the inside of one group of PCR reaction tubes can be observed in real time in the sample adding process, after the sample adding is finished, the two L-shaped rods are simultaneously pinched by two hands, the L-shaped rods are reversely rotated by one hundred and eighty degrees along the round block, as shown in figures 1 and 2, the group of PCR reaction tubes are retracted into the bottom box, the group of PCR reaction tubes after the sample adding is finished can be placed, and the above operations are repeated for seven times, alright carry out the application of sample with eight groups of PCR reaction bodys are whole, the PCR reaction body that the former group of application of sample was accomplished in a set of PCR reaction body application of sample in-process of back is inside through above operation indentation end box, the application of sample process alright observe with limit application of sample limit, the field of vision of observation is wider, thereby help the application of sample more, when taking out PCR reaction body, make eight connecting rods in proper order the upward movement can, it just takes out a set of PCR reaction body to rise a connecting rod, just can not be because every group PCR reaction body is in the coplanar and be difficult to the problem of taking out, the condition in the PCR reaction pipe is difficult to observe to the mount of present common PCR reaction pipe application of sample in-process, and be not convenient for the problem of.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged view of the structure A of FIG. 1 according to the present invention;

FIG. 3 is a side sectional view of the present invention;

FIG. 4 is a schematic structural view of the PCR reaction tube of the present invention when lifted;

fig. 5 is a top view of the top plate and the circular hole of the present invention.

In the figure: 1. a bottom case; 2. a PCR reaction tube body; 3. a top plate; 4. a circular hole; 5. a box cover; 6. a holder; 7. a connecting rod; 8. a long groove; 9. a fixing plate; 10. a hand buckle slot; 11. a circular tube; 12. a vertical rod; 13. fixing the rod; 14. a round block; 15. a rotating member; 16. an L-shaped rod.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-5, an auxiliary sample adding device for PCR reaction tubes comprises a bottom case 1 and eight sets of PCR reaction tubes 2, wherein the holes of the currently used hole plate are distributed in a 12 × 8 matrix, the present invention also adopts a 12 × 8 matrix distribution of round holes 4, the number of the PCR reaction tubes 2 in each set is twelve, the top of the bottom case 1 is fixedly connected with a top plate 3, the upper surface of the top plate 3 is provided with eight sets of round holes 4, the round holes 4 are distributed in a 12 × 8 matrix, the number of the round holes 4 in each set is twelve, the eight sets of PCR reaction tubes 2 are respectively inserted into the eight sets of round holes 4, the top of the bottom case 1 is provided with a case cover 5, the case cover 5 is clamped on the top of the bottom case 1 through a tight clamp, the inner wall of the case cover 5 is movably connected with the side of the top plate 3, the inner wall of the case cover 5 is in tight contact with the side of the, the bottom of each group of PCR reaction tube body 2 is provided with a group of supporting parts 6, the supporting parts 6 support the PCR reaction tube body 2, the number of the group of supporting parts 6 is twelve, the bottom of each group of supporting parts 6 is fixedly connected with a connecting rod 7, the connecting rods 7 fix the twelve supporting parts 6 in one group together to form a whole, the positions of the left side and the right side of the bottom box 1, which correspond to the two ends of the eight connecting rods 7, are respectively provided with an elongated slot 8, and the left end and the right end of the eight connecting rods 7 movably penetrate through the elongated slots 8 and extend to the two sides of the bottom box 1.

Specifically, the side surfaces of the periphery of the box cover 5 are fixedly connected with fixing plates 9, and hand fastening grooves 10 are formed in the outer sides of the fixing plates 9.

In this embodiment, the hand-clasping groove 10 of the fixed plate 9 side of the fixed connection around the box cover 5 is mainly convenient for the user to open the box cover 5, and the hand-clasping groove 10 is clasped by fingers, thereby playing the effect of conveniently opening the box cover 5.

Specifically, every the equal fixedly connected with pipe 11 of lower surface of connecting rod 7, every the equal sliding connection in inside of pipe 11 has montant 12, and connecting rod 7 sliding connection is on montant 12's surface, montant 12's both ends respectively with the top and the bottom fixed connection of end box 1 inner wall.

In this embodiment, the connecting rod 7 can move along the vertical rod 12 when moving, and the movement of the connecting rod 7 is guided.

Specifically, the number of the circular tubes 11 on the lower surface of each connecting rod 7 is two.

In this embodiment, as shown in fig. 1, the number of the circular tubes 11 on the lower surface of each connecting rod 7 is two, which can prevent the two ends of the connecting rod 7 from tilting up and down, thereby making the movement of the connecting rod 7 more stable.

Specifically, every the equal fixedly connected with dead lever 13 of upper surface at connecting rod 7 both ends, the top fixedly connected with round block 14 of dead lever 13, the surperficial rotation of round block 14 is connected with rotates piece 15, the side fixedly connected with L shape pole 16 that rotates piece 15.

In this embodiment, the L-shaped rod 16 is held by both hands, and the L-shaped rod 16 is lifted up, and when the connecting rod 7 moves to the state shown in fig. 4, the L-shaped rod 16 is not released by the fingers, and the L-shaped rod 16 is rotated by one hundred eighty degrees around the surface of the round block 14 while holding the L-shaped rod 16 until the L-shaped rod 16 is caught on the top of the bottom case 1, and the rising height of the connecting rod 7 is fixed.

Specifically, the upper surface of the support member 6 is concave, and the shape of the support member 6 is matched with the shape of the PCR reaction tube body 2.

In this embodiment, the support member 6 can support the PCR reaction tube body 2 well, and is more stable.

The utility model discloses a theory of operation and use flow: when the front box cover 5 is used and in an open state, the hand buckling grooves 10 on the side face of the fixing plate 9 fixedly connected with the periphery of the box cover 5 are mainly convenient for a user to open the box cover 5, fingers buckle the hand buckling grooves 10, when samples are added to the PCR reaction tube body 2, firstly, one connecting rod 7 of the eight connecting rods 7 is lifted, the specific operation is that the L-shaped rod 16 is pinched by two hands, the L-shaped rod 16 is lifted upwards at the same time, the circular tube 11 moves along the vertical rod 12 to guide the movement of the connecting rod 7 and prevent the deviation of the connecting rod 7, when the connecting rod 7 moves to the state shown in figure 4, the L-shaped rod 16 is not released by fingers in the process, the L-shaped rod 16 is rotated by one hundred eighty degrees around the surface of the circular block 14 while being pinched, until the L-shaped rod 16 is put on the top of the bottom box 1, the lifting height of the connecting rod 7 is fixed, and then, one group of the PCR, the support member 6 supports the PCR reaction tube 2, the PCR reaction tube 2 in one group is arranged on the support member 6, the sample can be added, the sample adding process is performed because most of the PCR reaction tube 2 is positioned above the top plate 3, the inside of the PCR reaction tube 2 in one group can be observed in real time in the sample adding process, after the sample adding is finished, two L-shaped rods 16 are simultaneously pinched by two hands, and meanwhile, the L-shaped rods 16 reversely rotate by one hundred eighty degrees along the round block 14, as shown in figures 1 and 2, the PCR reaction tube 2 in one group is retracted into the bottom box 1, the PCR reaction tube 2 in the other group can be placed, the above operations are repeated for seven times, the samples of the PCR reaction tube 2 in eight groups can be completely added, the PCR reaction tube 2 in the former group after the sample adding of the PCR reaction tube 2 in the latter group can be retracted into the bottom box 1 through the above operations, the sample adding process can be observed while adding the sample, and the observation field is wider, so that the sample adding is more facilitated; when taking out the PCR reaction tube body 2, make eight connecting rods 7 in proper order the upward movement can, rise a connecting rod 7 and just take out a set of PCR reaction tube body 2, just can not appear because of the problem that every group PCR reaction tube body 2 is in the coplanar and is difficult to take out.

Claims

1. The utility model provides an auxiliary sample adding device that PCR reaction was used, includes end box (1) and eight groups PCR reaction body (2), every group the quantity of PCR reaction body (2) is twelve, its characterized in that: the top of the bottom box (1) is fixedly connected with a top plate (3), eight groups of round holes (4) are formed in the upper surface of the top plate (3), the number of each group of round holes (4) is twelve, eight groups of PCR reaction tube bodies (2) are respectively inserted into the eight groups of round holes (4), the top of the bottom box (1) is provided with a box cover (5), the inner wall of the box cover (5) is movably connected with the side surface of the top plate (3), the bottom of each group of PCR reaction tube body (2) is provided with a group of supporting pieces (6), the number of the group of supporting pieces (6) is twelve, the bottom of each group of supporting pieces (6) is fixedly connected with a connecting rod (7), elongated slots (8) are formed in positions, corresponding to the two ends of the eight connecting rods (7), of the left side and the right side of the bottom box (1), and the left ends and the right ends of the eight connecting rods (7) movably penetrate through the elongated slots (8) and extend to the two sides of the bottom box (1).

2. The auxiliary sample adding device for the PCR reaction tube according to claim 1, wherein: the side all around of lid (5) all fixedly connected with fixed plate (9), buckle groove (10) have been seted up in the outside of fixed plate (9).

3. The auxiliary sample adding device for the PCR reaction tube according to claim 1, wherein: every the equal fixedly connected with pipe (11) of lower surface of connecting rod (7), every the equal sliding connection in inside of pipe (11) has montant (12), and connecting rod (7) sliding connection is on the surface of montant (12), the both ends of montant (12) respectively with the top and the bottom fixed connection of end box (1) inner wall.

4. The auxiliary sample adding device for the PCR reaction tube according to claim 3, wherein: the number of the round pipes (11) on the lower surface of each connecting rod (7) is two.

5. The auxiliary sample adding device for the PCR reaction tube according to claim 1, wherein: every the equal fixedly connected with dead lever (13) of upper surface at connecting rod (7) both ends, the top fixedly connected with circle piece (14) of dead lever (13), the surperficial rotation of circle piece (14) is connected with rotates piece (15), the side fixedly connected with L shape pole (16) that rotates piece (15).

6. The auxiliary sample adding device for the PCR reaction tube according to claim 1, wherein: the upper surface of the support piece (6) is concave, and the shape of the support piece (6) is matched with that of the PCR reaction tube body (2).