CN104651221A - Micro-insemination culture dish - Google Patents
Micro-insemination culture dish Download PDFInfo
- Publication number
- CN104651221A CN104651221A CN201310596544.4A CN201310596544A CN104651221A CN 104651221 A CN104651221 A CN 104651221A CN 201310596544 A CN201310596544 A CN 201310596544A CN 104651221 A CN104651221 A CN 104651221A
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- CN
- China
- Prior art keywords
- micro
- ware
- injection needle
- culture dish
- insemination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a micro-insemination culture dish, which includes a dish bottom, a dish pool side, and a dish bottom edge. The dish bottom includes an inclined plane with certain angle. By disposing the inclined micro-insemination culture dish bottom, a micro-injection needle front end and the dish bottom can still form an angle when the micro-injection needle front end is at a horizontal angle, and at the moment, if the needle tip touches the bottom, the rear part of the micro-injection needle front end does not touch the bottom to cause need tip upwarp and break away from the bottom sperms, so that sperms can be grabbed and pressed smoothly, thereby realizing normal micro-insemination operation. As the operation under the circumstance of a horizontal micro-injection needle front end is feasible, the micro-injection needle can be employed to conduct micro-insemination over against ova, thus avoiding the increased ovum disintegration risk caused by non-directly facing puncture in traditional methods.
Description
Technical field
The present invention relates to technical field of medical instruments, particularly a kind of culture dish carried out in supplementary reproduction medical science with using during the microscopic insemination of human sperm to ovum.
Background technology
At present, known microscopic insemination culture dish is horizontal bottom when operating.This is a kind of conventionally form that usual culture dish makes.But, the ovum disintegration rate of the 3%-8% that has an appointment in current microscopic insemination operation.Cause some normal ovums dead.This just has the pressure of sizable risk of failure for some cases that natively ovum number is few.The reason of this risk is caused to have ovum factor and operation factors.The disintegration with regard to easy puncture when the after birth of ovum is more fragile, disadvantageous operating method then can aggravate the generation of this risk.This disadvantageous operating method is exactly mainly that the penetration damage that causes of the inclination of microscopical puncture pin strengthens.
In traditional operation, microscopical puncture pin fore-end needs the state be installed into obliquely.This is because microscopical puncture pin front end be not completely all sized by kapillary, be tapered needle-like.When this pin horizontal positioned, when pressing close to crawl sperm at the bottom of ware with it, what touch the end at first will not be needle point, but the rear portion of this front end, rear portion is touched the end and is caused tip to upwarp on the contrary, makes needle point more cannot contact and capture sperm, more cannot push down sperm with needle point and carry out immobilization of spermatozoa operation.And these two operations are the necessary processes of microscopic insemination.So, in order to avoid the operational issue that this heterogeneity front end of injection needle is brought, injection needle is then mounted to state obliquely by conventional microscopic insemination method, when this makes needle point touch end operation sperm, the rear portion of entry needle front end is still the unsettled state not touching the end, ensure that operation is carried out.
But, this angle of inclination then can produce the right pressure of anon-normal to ovum when puncturing ovum, just ovum zona pellucida can be worn out when puncture needs are oppressed largely, the consistent moment when zona pellucida is the worse for wear, ovum after birth is subject to suddenly and immediately compared with the puncture of great, ovum after birth is easily the worse for wear immediately, instead of not wearing out under normal circumstances.This can cause the increasing of ovum disintegration risk subsequently.
Summary of the invention
In order to solve at present the above-mentioned problem that microscopic insemination culture dish in operation exists, now provide a kind of microscopic insemination culture dish.Concrete technical scheme is as follows:
A kind of microscopic insemination culture dish, comprises at the bottom of ware, ware pool side, ware root edge, includes the scarp of certain angle at the bottom of described ware.
Preferably, be an integral inclined plane at the bottom of described ware, described ware bottom surface and described ware root edge place plane are at an angle.
Preferably, described ware root edge place plane and ware pool side place plane parallel.
Preferably, described ware bottom surface is provided with projection, and described projection is the scarp with radian or the gradient.
Compared with prior art, the microscopic insemination culture dish that technique scheme provides has the following advantages: have at the bottom of inclination microscopic insemination culture dish ware by arranging, injection needle is made to revert to level angle, at this moment when needle point touches the end, the end, can not be touched in rear portion, injection needle front end, can normal running microscopic insemination.This just just can do microscopic insemination to ovum with injection needle, and the anon-normal avoiding traditional method increases the risk of ovum disintegration to puncture.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme in the embodiment of the present invention, below the accompanying drawing used required in describing embodiment is briefly described, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the microscopic insemination culture dish structural representation provided in the embodiment of the present invention;
Fig. 2 is the microscopic insemination culture dish operational stage schematic diagram provided in the embodiment of the present invention.
Embodiment
For making the object, technical solutions and advantages of the present invention clearly, below in conjunction with accompanying drawing, embodiment of the present invention is described further in detail.
As shown in Figure 1, embodiments provide a kind of microscopic insemination culture dish, to comprise at the bottom of ware 1, ware pool side 3, ware root edge 2,1 scarp including certain angle at the bottom of ware.At the bottom of ware, 1 is an integral inclined plane, and 1 and ware root edge 2 place plane at the bottom of ware are at an angle.Ware root edge 2 place plane and ware pool side 3 place plane parallel.Ware bottom surface is provided with projection, and projection is the scarp with radian.
As shown in Figure 2, owing to being scarp bottom culture dish, have at the bottom of inclination microscopic insemination culture dish ware, make injection needle front end when becoming level angle still and at the bottom of ware between have angle, if needle point at this moment touches the end, then rear portion, injection needle front end can not touch the end and cause needle point upwarp depart from be in bottom sperm, thus can capture sperm smoothly and press draw, realize normal microscopic insemination operate.Because this injection needle front end becomes level angle operation feasible, just make it possible to just do microscopic insemination to ovum with injection needle, the anon-normal avoiding traditional method increases the risk of ovum disintegration to puncture.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (4)
1. a microscopic insemination culture dish, comprises at the bottom of ware, ware pool side, ware root edge, it is characterized in that, includes the scarp of certain angle at the bottom of described ware.
2. microscopic insemination culture dish according to claim 1, is characterized in that, is an integral inclined plane at the bottom of described ware, and described ware bottom surface and described ware root edge place plane are at an angle.
3. microscopic insemination culture dish according to claim 2, is characterized in that, described ware root edge place plane and ware pool side place plane parallel.
4. microscopic insemination culture dish according to claim 1, is characterized in that, described ware bottom surface is provided with projection, and described projection is the scarp with radian or the gradient.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310596544.4A CN104651221A (en) | 2013-11-21 | 2013-11-21 | Micro-insemination culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310596544.4A CN104651221A (en) | 2013-11-21 | 2013-11-21 | Micro-insemination culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104651221A true CN104651221A (en) | 2015-05-27 |
Family
ID=53242874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310596544.4A Pending CN104651221A (en) | 2013-11-21 | 2013-11-21 | Micro-insemination culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104651221A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108546633A (en) * | 2018-04-17 | 2018-09-18 | 贵州省中国科学院天然产物化学重点实验室 | A kind of auxiliary device that culture dish uses |
| CN108913600A (en) * | 2018-08-16 | 2018-11-30 | 杭州德适生物科技有限公司 | A kind of microscopic insemination operation ware |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102212468A (en) * | 2010-01-18 | 2011-10-12 | 贝克顿·迪金森公司 | Container assembly |
| CN202030741U (en) * | 2011-02-14 | 2011-11-09 | 重庆大学 | Culture dish containing micro spherical array |
| CN202175676U (en) * | 2011-08-22 | 2012-03-28 | 唐亚军 | Biochemical culture dish |
| CN203048938U (en) * | 2013-01-16 | 2013-07-10 | 于凤丽 | Novel culture dish |
| CN203212574U (en) * | 2013-03-15 | 2013-09-25 | 黄冈职业技术学院 | Bacterial colony point selecting and counting culture dish |
-
2013
- 2013-11-21 CN CN201310596544.4A patent/CN104651221A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102212468A (en) * | 2010-01-18 | 2011-10-12 | 贝克顿·迪金森公司 | Container assembly |
| CN202030741U (en) * | 2011-02-14 | 2011-11-09 | 重庆大学 | Culture dish containing micro spherical array |
| CN202175676U (en) * | 2011-08-22 | 2012-03-28 | 唐亚军 | Biochemical culture dish |
| CN203048938U (en) * | 2013-01-16 | 2013-07-10 | 于凤丽 | Novel culture dish |
| CN203212574U (en) * | 2013-03-15 | 2013-09-25 | 黄冈职业技术学院 | Bacterial colony point selecting and counting culture dish |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108546633A (en) * | 2018-04-17 | 2018-09-18 | 贵州省中国科学院天然产物化学重点实验室 | A kind of auxiliary device that culture dish uses |
| CN108913600A (en) * | 2018-08-16 | 2018-11-30 | 杭州德适生物科技有限公司 | A kind of microscopic insemination operation ware |
| WO2020034815A1 (en) * | 2018-08-16 | 2020-02-20 | 杭州德适生物科技有限公司 | Micro-insemination operation container |
| CN108913600B (en) * | 2018-08-16 | 2023-06-27 | 杭州德适生物科技有限公司 | Microscopic insemination operation dish |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150527 |