CN2822540Y - Sperm sorting vessel - Google Patents
Sperm sorting vessel Download PDFInfo
- Publication number
- CN2822540Y CN2822540Y CN 200520016391 CN200520016391U CN2822540Y CN 2822540 Y CN2822540 Y CN 2822540Y CN 200520016391 CN200520016391 CN 200520016391 CN 200520016391 U CN200520016391 U CN 200520016391U CN 2822540 Y CN2822540 Y CN 2822540Y
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- sperm
- ware
- seminal fluid
- concentrated solution
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Abstract
The utility model provides a sperm sorting vessel which can be used for still holding sperms, sperm concentrated solution, etc. which is provided with a sperm still holding part of sperm liquids and easily used for holding sperms or sperm concentrated solution with no air bubbles. A concave sperm still holding part is arranged on the bottom face of the sperm sorting vessel. The utility model is characterized in that the sperm still holding part comprises an approximately horizontal bottom and an inclined side-wall part, wherein an angle which is formed by the perpendicular line of the bottom of the side-wall face is five degrees to 45 DEG.
Description
Technical field
The present invention relates to a kind of sperm that can choose the high sperm of motor function and choose ware.
Background technology
In recent years, as infertile treatment, the treatment of being undertaken by supplementary reproduction medical treatment such as in vitro fertilization-embryo transfer (Assisted Reproductive Techology:ART) has been subjected to people's attention.For example, in vitro fertilization-embryo transfer, use the seminal fluid that concentrates sperm, be used for artificial inseminations such as microscopic insemination.In addition, for example also use the sperm that manually to take to inject the depths in uterus and carry out test-tube method.Be used for this test-tube sperm and can use spissated seminal fluid, swim (Swim up) method but wish to use, this is a kind of method of using normal motion sperm and taking the higher sperm of exercise performance.
Play the medical apparatus that uses in (Swim up) method as above-mentioned trip, for example proposed a kind of recess that is provided with in the bottom and formed physical barrier with motion to sperm, thus the ware of selectively sperm of swimming out of the tubular recess being taked (patent documentation 1).
Therefore, the angle that is become with the bottom by the side wall surface that makes recess be the right angle to acute angle, can control freely spreading of sperm well.
The sperm concentrated solution uses the sperm of ormal weight to guarantee to stipulate.It is usually following and use that sperm is chosen ware: inject than the concavity seminal fluid and leave standstill seminal fluid that the spatial cubical content of portion Duos or sperm concentrated solution etc., sperm concentrated solution etc. is exceeded until the ware bottom surface.Yet spissated seminal fluid is because there is the high situation of viscosity in individual differences.Therefore, the angle that this side wall surface is become with the bottom is right angle during to acute angle, if use the high sperm concentrated solution of viscosity, then is easy to generate bubble in the corner of side wall surface and bottom.If produce such bubble, then become the bottom side that leaves standstill portion at above-mentioned seminal fluid and have bubble, and on this bubble, have the situation of sperm concentrated solution.
When having the sperm concentrated solution on bubble, the concentrated seminal fluid of configuration is more near the side surface direction of ware, thereby the sperm that occurs the exercise performance difference easily also can be swum over to the lateral situation of ware.When producing bubble, situation about shortening to the beeline at the interface of sperm concentrated solution and culture fluid from the position of the close bottom of sperm concentrated solution appears.In addition, when producing bubble, because the area at sperm concentrated solution and the interface of culture fluid increases, thereby the situation of quantity increase of the sperm at this interface appears also being present in.In these cases, because the distance that sperm moves about in the high sperm concentrated solution of ratio of viscosities culture fluid reduces, thereby the sperm of exercise performance difference becomes easy near swimming over to the ware side.The generation of above-mentioned bubble just becomes the obstacle of taking the high sperm of exercise performance.
Patent documentation 1: the spy opens 2003-289850
Summary of the invention
As removing this method that results from the bubble of this corner portions located, for example can use the rod in thin footpath or pin to carry out stirring method.
Yet, when implementing described measure, the situation of sperm may occur damaging, and may spermatiferously freely spread.This measure is not preferred as the reproduction medical treatment, if can avoid then needing to avoid.
The object of the present invention is to provide a kind of sperm to choose ware, can leave standstill portion being used to leave standstill the seminal fluid that seminal fluid, sperm concentrated solution, sperm suspension etc. contain the liquid of sperm, do not produce bubble and seminal fluid easily is set or the sperm concentrated solution.
Present inventors are through wholwe-hearted research, found that, choose ware by using following a kind of sperm, can not produce bubble and easily leave standstill seminal fluid or sperm concentrated solution in the seminal fluid portion of leaving standstill, thereby make the present invention, this sperm is chosen ware, the seminal fluid that has concavity in the bottom surface leaves standstill portion, it is characterized in that this seminal fluid portion of leaving standstill comprises the bottom and the angled side walls portion of approximate horizontal, the angle that the vertical line of this bottom is become with this side wall surface is 5～45 °.
The sperm of the application of the invention is chosen ware, can easily leave standstill seminal fluid or sperm concentrated solution, thereby the loss of the seminal fluid that can not occur taking.Therefore, the operation of supplementary reproduction medical treatment also becomes easily, also can reduce the operation of taking seminal fluid from the sperm supplier, thereby can also alleviate sperm supplier's burden.
Description of drawings
Fig. 1 is the general perspective that sperm of the present invention is chosen an embodiment of ware.
Fig. 2 is the longitudinal section that the sperm of Fig. 1 is chosen ware.
Fig. 3 (a) is the sketch map that the sperm of Fig. 1 is chosen ware middle reaches time-out, (b) is the sketch map that in the past sperm is chosen ware middle reaches time-out.
The specific embodiment
The present invention will be described below to use accompanying drawing, but the present invention is not limited to these embodiments shown in the drawings.
Fig. 1 is the general perspective that sperm of the present invention is chosen an embodiment of ware.Fig. 2 is the longitudinal section that the sperm of Fig. 1 is chosen ware.
In Fig. 1, choose in the ware 1 at sperm, seminal fluid is left standstill portion 2 be arranged on the bottom surface 3 that this sperm is chosen ware 1.
By utensils such as syringes seminal fluid, sperm concentrated solution or sperm suspension being statically placed in seminal fluid leaves standstill in the portion 2.Then as shown in Figure 3, the culture fluid that will Duo than the seminal fluid equivalent that leaves standstill does not mix with seminal fluid etc. and is disposed on the seminal fluid that leaves standstill etc., and then the oil that will be used to avoid evaporating is configured in this culture fluid.
In the drawings, the above-mentioned seminal fluid side wall portion 5 that leaves standstill portion forms conical surface-shaped.The seminal fluid of concavity leaves standstill portion 2, has // the circular open portion of diameter 2～10mm, has the volume of 0.01～0.2mL, preferred 40～80 μ L as inner space.Be used to take from the seminal fluid to the needed amount of sperm of culture fluid trip (Swim up) such as seminal fluid by above-mentioned volume being in the above-mentioned scope, can accommodating, and the height from the bottom to the peristome is the height that is suitable for selecting the good sperm of mobility.
As shown in Figure 2, seminal fluid leaves standstill bottom 4 and the angled side walls portion 5 that portion 2 has approximate horizontal.Because the angle θ that the vertical line s of bottom 4 and the extended line t of side wall portion 5 are become is in 5～45 ° of scopes, preferably in 5～30 ° scope, air is difficult to enter the corner of bottom and side wall portion, and, do not have sperm because horizontal the diffusion naturally and from the situation of seminal fluid to the culture fluid transition because angle θ is not excessive yet.In the drawings, side wall portion is from the linearity of peristome to the bottom on the longitudinal section.Above-mentioned side wall portion can be convex or concavity laterally.When side wall portion is convex or concavity, can be with the line that connects the peristome side end of side wall portion and bottom side end as above-mentioned extended line, and calculate angle θ.
As mentioned above, seminal fluid etc., culture fluid and oil are configured in sperm in turn to be chosen in the ware, left standstill under the room temperature 30～60 minutes, the sperm that then well moves moves to culture fluid from seminal fluid etc., take when using handstand microscope etc. that the sperm that moves to the culture fluid on the bottom surface 3 etc. is observed, can take the sperm that well moves.When using sperm of the present invention to choose ware, shown in Fig. 3 (a),, thereby take the good sperm of exercise performance easily because the sperm of the motion of the gravity of putting up a resistance is more.
Yet using angle θ is 0 ° sperm in the past when choosing ware, because air enters the corner portions located that seminal fluid leaves standstill portion easily, thereby is easy to generate bubble.At this moment, shown in Fig. 3 (b), originally should with the sperm concentrated solution 7 of bottom connection ' because bubble 12 and on short transverse, rising, be present in bubble 12 and sperm concentrated solution 7 ' the sperm of near interface, its to sperm concentrated solution 7 ' and culture fluid 8 ' the beeline at interface and the situation reduced in comparison of Fig. 3 (a).In addition, sperm concentrated solution 7 ' and culture fluid 8 ' the area at interface increase.Therefore, the short sperm of the distance of moving about in the high sperm concentrated solution of viscosity increases, thereby when using in the past sperm to choose ware, can produce the situation of the sperm of having taked the exercise performance difference.
Sperm of the present invention is chosen ware, if possess the bottom surface of the culture fluid of taking operation to become to be easy to capacity that can splendid attire makes sperm, the sperm that a plurality of concavities then also can be set leaves standstill portion.When the above-mentioned sperm portion of leaving standstill was 1, choosing the diameter that can make the bottom surface in the ware at above-mentioned sperm was 20～80mm.And this moment, the outside wall portions 6 that above-mentioned sperm is chosen ware is not specially limited, and can make it highly is 2～5mm.
Sperm of the present invention is chosen ware, its material is not specially limited, and can use glass or plastics, owing to can carry out electron beam sterilization, and observe the motion of sperm easily, thereby preferably use with polystyrene, polyethylene and polyamide plastic material as the high transparent of representative.
The sperm of the application of the invention is chosen ware, can easily prevent the generation of the bubble that produces when seminal fluid injects in the seminal fluid portion of leaving standstill, thereby can prevent that sperm from easily moving in the culture fluid, thereby can take the good sperm of exercise performance efficiently.Therefore, sperm of the present invention is chosen ware and is gone for the supplementary reproduction medical treatment.
Claims (2)
1. a sperm is chosen ware, and the seminal fluid that has concavity in the bottom surface leaves standstill portion, it is characterized in that, this seminal fluid portion of leaving standstill comprises the bottom and the angled side walls portion of approximate horizontal, and the angle that the vertical line of this bottom is become with this side wall surface is 5～45 °.
2. sperm as claimed in claim 1 is chosen ware, it is characterized in that, the described seminal fluid portion of leaving standstill has the circular open portion that diameter is 2～10mm, and the volume with 0.01～0.2mL is as inner space.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520016391 CN2822540Y (en) | 2005-04-28 | 2005-04-28 | Sperm sorting vessel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520016391 CN2822540Y (en) | 2005-04-28 | 2005-04-28 | Sperm sorting vessel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2822540Y true CN2822540Y (en) | 2006-10-04 |
Family
ID=37031648
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520016391 Expired - Lifetime CN2822540Y (en) | 2005-04-28 | 2005-04-28 | Sperm sorting vessel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2822540Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101805691A (en) * | 2010-04-30 | 2010-08-18 | 邱毅 | Inner sleeve type separation screening sperm tube |
| CN101156823B (en) * | 2007-09-29 | 2011-07-06 | 曾勇 | Seminal fluid collecting cup and its using method |
| CN104823966A (en) * | 2015-05-19 | 2015-08-12 | 上海交通大学医学院附属第九人民医院 | Trace sperm cryopreservation carrier and corresponding freezing and thawing methods thereof |
| CN106754254A (en) * | 2017-03-21 | 2017-05-31 | 明光现代农业科技合作推广服务中心 | A kind of animal capacitated sperm collects ware |
| CN108913600A (en) * | 2018-08-16 | 2018-11-30 | 杭州德适生物科技有限公司 | A kind of microscopic insemination operation ware |
-
2005
- 2005-04-28 CN CN 200520016391 patent/CN2822540Y/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156823B (en) * | 2007-09-29 | 2011-07-06 | 曾勇 | Seminal fluid collecting cup and its using method |
| CN101805691A (en) * | 2010-04-30 | 2010-08-18 | 邱毅 | Inner sleeve type separation screening sperm tube |
| CN101805691B (en) * | 2010-04-30 | 2012-10-17 | 山东省计划生育科学技术研究所 | Inner sleeve type separation screening sperm tube |
| CN104823966A (en) * | 2015-05-19 | 2015-08-12 | 上海交通大学医学院附属第九人民医院 | Trace sperm cryopreservation carrier and corresponding freezing and thawing methods thereof |
| CN106754254A (en) * | 2017-03-21 | 2017-05-31 | 明光现代农业科技合作推广服务中心 | A kind of animal capacitated sperm collects ware |
| CN108913600A (en) * | 2018-08-16 | 2018-11-30 | 杭州德适生物科技有限公司 | A kind of microscopic insemination operation ware |
| CN108913600B (en) * | 2018-08-16 | 2023-06-27 | 杭州德适生物科技有限公司 | Microscopic insemination operation dish |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CX01 | Expiry of patent term |
Expiration termination date: 20150428 Granted publication date: 20061004 |