CN206783656U - The orifice plate ice chest of quantitative fluorescent PCR 96 - Google Patents

The orifice plate ice chest of quantitative fluorescent PCR 96 Download PDF

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Publication number
CN206783656U
CN206783656U CN201720552709.1U CN201720552709U CN206783656U CN 206783656 U CN206783656 U CN 206783656U CN 201720552709 U CN201720552709 U CN 201720552709U CN 206783656 U CN206783656 U CN 206783656U
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China
Prior art keywords
ice chest
orifice plate
centimetres
halfpace
quantitative fluorescent
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CN201720552709.1U
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Chinese (zh)
Inventor
吴少鸿
李欣
刘奇志
王祖雄
付剑锋
李胜果
周文根
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Shenzhen Maine Medical Laboratory Laboratory
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Xiamen Beauty Biotechnology Co Ltd
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Abstract

The utility model discloses a kind of orifice plate ice chest of quantitative fluorescent PCR 96, including box body, the spill receiving portion for accommodating 96 orifice plates is provided with the cartridge upper surface, with with the spill receiving portion close to and higher than the spill receiving portion halfpace portion, an arc nest portion is respectively arranged with the corresponding two side of the spill receiving portion, the halfpace portion is provided with the big hole portion more than 96 orifice plate apertures, the spill receiving portion accounts for 3/4ths of cartridge upper surface, the halfpace portion accounts for a quarter of cartridge upper surface, and periphery also has outer portion above the box body.The orifice plate ice chest of quantitative fluorescent PCR 96 provided by the utility model, solve no 96 orifice plate ice chests in the prior art, the problem of control of 96 orifice plate low temperature is unstable, sample-adding is inconvenient for operation is kept in PCR courses of reaction.

Description

The orifice plate ice chest of quantitative fluorescent PCR 96
Technical field
It the utility model is related to zymetology or microbiology engineering device technique field, more particularly to a kind of quantitative fluorescent PCR 96 Orifice plate ice chest.
Background technology
PCR (Polymerase Chain Reaction), abbreviation PCR, is a kind of molecular biology skill Art, for amplifying specific DNA fragmentation.The special DNA replication dna of in vitro can be regarded as.In quantitative fluorescent PCR reaction, enzyme Activity influences very big on the efficiency of PCR reactions, it usually needs PCR system is stored in 0~4 DEG C of environment.Generally, in laboratory Carry out in PCR system process for preparation, sample-adding is aided in using the biological ice box in 38 holes shown in Fig. 1, i.e., 96 orifice plates are placed on ice chest On so that 96 hole PCR plates can be in cryogenic conditions during sample-adding, it is ensured that the activity of enzyme, but existing 96 hole PCR plate is with showing There is ice chest to be mutually matched, as shown in Fig. 2 96 hole PCR plates are placed on ice chest and can not completely attached to, preferable cryogenic conditions Difficulty reaches, and 96 hole PCR plates are put very unstable on ice chest, can be slided often, be have impact on feed rate in experimentation, cause Operating efficiency reduces.Also have using method as shown in Figure 3 and Figure 4,96 orifice plate seats are positioned on ice chest, then by 96 hole PCR Plate is fixed on 96 orifice plate seats, though solve the problems, such as stability, because 96 orifice plate seats by 96 hole PCR plates and ice chest every Open, cause cryogenic conditions to be extremely difficult to, influence experimental result.
Utility model content
The utility model purpose is to provide a kind of orifice plate ice chest of quantitative fluorescent PCR 96, solves in the prior art without 96 holes Plate ice chest, the problem of control of 96 orifice plate low temperature is unstable, sample-adding is inconvenient for operation is kept in PCR courses of reaction.
The utility model solves technical problem and adopted the following technical scheme that:A kind of orifice plate ice chest of quantitative fluorescent PCR 96, bag Include box body, the cartridge upper surface be provided with accommodate 96 orifice plates spill receiving portion, and with the spill receiving portion close to and Higher than the halfpace portion of the spill receiving portion, the spill receiving portion is respectively arranged with an arc nest on corresponding two side Portion, the halfpace portion are provided with the big hole portion more than 96 orifice plate apertures, the spill receiving portion account for cartridge upper surface four/ Three, the halfpace portion accounts for a quarter of cartridge upper surface, and periphery also has outer portion above the box body.
Preferably, the ice bin size is wide 10 centimetres to be long 17.3 centimetres, high 6.5 centimetres, and the spill accommodates minister It is 12.3 centimetres, wide 8.3 centimetres, it is deep 2 centimetres.
Preferably, the big hole portion aperture is 1.2 centimetres~1.4 centimetres, and hole depth is 3 centimetres, for placing 5 milliliters of EP pipes Or 2 milliliters of EP pipes.
Preferably, 6-8 big hole portions are provided with the halfpace portion.
Preferably, in addition to a halfpace portion supporting plate, halfpace portion supporting plate is consistent with halfpace portion surface size, sets thereon There is the circular hole portion corresponding with macropore portion position, the Circularhole diameter in the circular hole portion is less than big hole portion upper surface circular aperture.
Preferably, the ice chest also has matching lid, and ice chest outer portion also has on corresponding 96 orifice plates Horizontal stroke, the numeral or letter of vertical hole position.
The utility model has the advantages that:
The orifice plate ice chest of the utility model quantitative fluorescent PCR 96, the orifice plates of receiving PCR 96 being bonded completely are both effective to protect Sample temperature has been held, but it is easy to operation, ensure that having prepared PCR reaction systems during being loaded is in low temperature environment, the activity of enzyme It will not reduce;On the other hand 96 hole PCR plates can be secured firmly on ice chest, ensure sample-adding during 96 orifice plates will not slide or Because being loaded too quickly overturn;Also there is the big hole portion of other sizes hole position in the halfpace portion of setting, other apertures can be accommodated simultaneously EP is managed, it is not necessary to is prepared ice chest in addition, is saved experimental article and space;The arc nest portion of setting is easy to take out the orifice plates of PCR 96, Easily operate and do not influence its steadiness on ice chest box body.
Brief description of the drawings
Fig. 1 is the hole ice chest schematic diagram of prior art 38;
Fig. 2 is placed in the schematic diagram shown in Fig. 1 on ice chest for the orifice plate of prior art 96;
Fig. 3 is the schematic diagram that the orifice plate seat of prior art 96 is positioned on ice chest;
Fig. 4 is the schematic diagram that the orifice plate of prior art 96 is fixed on 96 orifice plate seats;
Fig. 5 is the structural representation of the orifice plate ice chest of 1 quantitative fluorescent PCR of the utility model embodiment 96;
Fig. 6 is Fig. 5 AA sectional views;
Fig. 7 is to prevent the 96 orifice plate side schematic views in the spill receiving portion of the present embodiment ice chest;
Mark is illustrated as in figure:1-96 orifice plates;2-96 orifice plate seats;3- box bodys;301- outers portion;4- spill receiving portions; 401- arc nests portion;5- halfpaces portion;The big hole portions of 501-.
Embodiment
The technical solution of the utility model is further elaborated with reference to embodiment and accompanying drawing.
Embodiment 1
A kind of orifice plate ice chest of quantitative fluorescent PCR 96 is present embodiments provided, as shown in Figure 5 and Figure 6, including box body 3, The upper surface of box body 3 is provided with the spill receiving portion 4 for accommodating 96 orifice plates, and with the spill receiving portion 4 close to and be higher than institute The halfpace portion 5 of spill receiving portion 4 is stated, an arc nest portion is respectively arranged with the corresponding two side of the spill receiving portion 4 401, the halfpace portion 5 is provided with the big hole portion 501 more than 96 orifice plate apertures, and the spill receiving portion 4 accounts for the upper surface of box body 3 3/4ths, the halfpace portion 5 accounts for a quarter of the upper surface of box body 3, and the top periphery of box body 3 also has outer portion 301.What the orifice plates of PCR 96 were bonded completely is placed on the spill receiving portion 4 of the present embodiment ice chest, and sample-adding process can be effectively ensured Middle preparation PCR reaction systems are in low temperature environment all the time, and the activity of enzyme will not reduce;96 hole PCR plates are secured firmly to by and can On ice chest, 96 orifice plates will not slide or because being loaded too quickly overturn during ensureing sample-adding;Meanwhile also have in the halfpace portion 5 of setting There is the big hole portion 501 of other sizes hole position, the EP pipes in other apertures can be accommodated simultaneously, it is not necessary to prepare ice chest in addition, save experiment Articles for use and space;The arc nest portion 402 of setting is easy to take out the orifice plates of PCR 96, easily operates and does not influence it in ice chest box Steadiness on body.Box body 3 can be arranged in pairs or groups matching lid, and firm effect is played by outer portion 301.
In the present embodiment, the size of ice chest 3 is wide 10 centimetres to be long 17.3 centimetres, high 6.5 centimetres, and the spill accommodates 12.3 centimetres of minister, 8.3 centimetres of money are deep 2 centimetres.The aperture of big hole portion 501 is 1.2 centimetres~1.4 centimetres, and hole depth is 3 lis Rice, for placing 5 milliliters of EP pipes or 2 milliliters of EP pipes.
In the present embodiment, 6-8 big hole portion 501 is provided with the halfpace portion 5.The quantity of the big hole portion 501 is set Ice chest size design can be adjusted by their entirety, the size of big hole portion 501, which is also satisfied with, prepares what is be related to during PCR reaction systems Reagent Tube or the placement for being loaded pipe.
Further, the present embodiment also includes a halfpace portion supporting plate, halfpace portion supporting plate and the surface size one of halfpace portion 5 Cause, be provided with the circular hole portion corresponding with the big position of hole portion 501, the Circularhole diameter in the circular hole portion is less than big hole portion 501 Upper surface circular aperture.Put when needing placement small-bore Reagent Tube or sample-adding Guan Shike simultaneously by halfpace portion supporting plate to fix Put.
Further, ice chest described in the present embodiment also has matching lid, and ice chest outer portion also has corresponding The numeral or letter of horizontal stroke, vertical hole position on 96 orifice plates.It is easy to identify, records hole position on 96 orifice plates, beneficial to experimentation In operation.
Finally it should be noted that:Above example is only to illustrate the technical solution of the utility model, rather than its limitations; Although the utility model is described in detail with reference to the foregoing embodiments, it will be understood by those within the art that: It can still modify to the technical scheme described in foregoing embodiments, or which part technical characteristic is carried out etc. With replacement;And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the utility model technology The spirit and scope of scheme.

Claims (6)

1. a kind of orifice plate ice chest of quantitative fluorescent PCR 96, it is characterised in that including box body, the cartridge upper surface is provided with appearance Receive the spill receiving portions of 96 orifice plates, and with the spill receiving portion close to and higher than the spill receiving portion halfpace portion, it is described An arc nest portion is respectively arranged with the corresponding two side of spill receiving portion, the halfpace portion is provided with more than 96 orifice plate apertures Big hole portion, the spill receiving portion accounts for 3/4ths of cartridge upper surface, the halfpace portion account for cartridge upper surface four/ One, box body top periphery also has outer portion.
2. the orifice plate ice chest of a kind of quantitative fluorescent PCR 96 according to claim 1, it is characterised in that the ice bin size is Long 17.3 centimetres, wide 10 centimetres, high 6.5 centimetres, the spill accommodates 12.3 centimetres of minister, wide 8.3 centimetres, deep 2 centimetres.
A kind of 3. orifice plate ice chest of quantitative fluorescent PCR 96 according to claim 1, it is characterised in that the big hole portion aperture For 1.2 centimetres~1.4 centimetres, hole depth is 3 centimetres, for placing 5 milliliters of EP pipes or 2 milliliters of EP pipes.
4. the orifice plate ice chest of a kind of quantitative fluorescent PCR 96 according to claim 1, it is characterised in that set in the halfpace portion It is equipped with 6-8 big hole portions.
5. the orifice plate ice chest of a kind of quantitative fluorescent PCR 96 according to claim 1, it is characterised in that also including a halfpace portion Supporting plate, halfpace portion supporting plate is consistent with halfpace portion surface size, is provided with the circular hole portion corresponding with macropore portion position, The Circularhole diameter in the circular hole portion is less than big hole portion upper surface circular aperture.
6. the orifice plate ice chest of a kind of quantitative fluorescent PCR 96 according to claim 1, it is characterised in that the ice chest also has Matching lid, ice chest outer portion also have the horizontal stroke corresponded on 96 orifice plates, the numeral or letter of vertical hole position.
CN201720552709.1U 2017-05-18 2017-05-18 The orifice plate ice chest of quantitative fluorescent PCR 96 Active CN206783656U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720552709.1U CN206783656U (en) 2017-05-18 2017-05-18 The orifice plate ice chest of quantitative fluorescent PCR 96

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720552709.1U CN206783656U (en) 2017-05-18 2017-05-18 The orifice plate ice chest of quantitative fluorescent PCR 96

Publications (1)

Publication Number Publication Date
CN206783656U true CN206783656U (en) 2017-12-22

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Country Status (1)

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CN (1) CN206783656U (en)

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Effective date of registration: 20180115

Address after: 518118 Guangdong province Shenzhen City Ping mountain industrial park, Pingshan Street Haike Hing Building 7 floor B District 02

Patentee after: Shenzhen Maine Medical Laboratory Laboratory

Address before: 361015 room 1402C, room 1402C, No. 619, Simi waterway, Huli District, Xiamen, Fujian

Patentee before: Xiamen beauty Biotechnology Co., Ltd.