CN209081911U - Tissue culture plate - Google Patents

Tissue culture plate Download PDF

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Publication number
CN209081911U
CN209081911U CN201821626194.6U CN201821626194U CN209081911U CN 209081911 U CN209081911 U CN 209081911U CN 201821626194 U CN201821626194 U CN 201821626194U CN 209081911 U CN209081911 U CN 209081911U
Authority
CN
China
Prior art keywords
tissue culture
culture plate
pipette tips
hole
diversion trench
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201821626194.6U
Other languages
Chinese (zh)
Inventor
邢凤
高庆华
祁鑫
李闯
李庆瑾
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tarim University
Original Assignee
Tarim University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tarim University filed Critical Tarim University
Priority to CN201821626194.6U priority Critical patent/CN209081911U/en
Application granted granted Critical
Publication of CN209081911U publication Critical patent/CN209081911U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model discloses a kind of tissue culture plates, the cultivation plate hole that it includes tissue culture plate ontology, is arranged on tissue culture plate ontology, it is characterised in that it further includes the pipette tips card hole that the intrinsic diversion trench of tissue culture plate is arranged in, is provided with tissue culture plate body top surface;The diversion trench has the ramped bottom surface of a cultivation plate hole for making to be loaded liquid flow direction;One end that the diversion trench is located at ramped bottom surface lower position is connected to the side wall of cultivation plate hole, and one end that the diversion trench is located at ramped bottom surface higher position is connected to pipette tips card hole.The utility model born of the same parents culture plate can be in order to be loaded, and can be effectively controlled the flow velocity of sample-adding sample liquid, makes the cell monolayer in tissue culture plate be not easy to be destroyed, will not pollute pipette tips, and the operating efficiency of experimentation addition sample liquid greatly improved.

Description

Tissue culture plate
Technical field
The utility model relates to cell culture apparatus, and in particular to a kind of tissue culture plate.
Background technique
Cell culture, which refers to, simulates tumor growth environment for the cell for acquiring in-vivo tissue, is placed on sterile, certain nutrition At condition, suitable temperature and pH value, make its growth, breeding, and maintain a kind of technology of its structure and function.Cell culture Morphosis, vital movement and its variation of living cells can be directly observed, it is to cytology, science of heredity, virology, immunologic Research and application play a significant role.Cell culture is usually required using cell culture apparatus, such as cell incubator, cell culture Plate etc..Wherein tissue culture plate is the biological utensil for being most commonly used for culture cell, it should be noted that in cell culture The quantity, quality of cell monolayer and uniformity coefficient can be directly related with subsequent experimental result in plate, therefore during the experiment It will have a direct impact on the authenticity and reliability of subsequent experimental result to the destruction of the cell monolayer in tissue culture plate.
In general, the attached cell being grown in tissue culture plate be not grown in Tissue Culture Flask it is adherent securely, and It is easy to make cell monolayer fall off because sample liquid is added during the experiment, causes experimental results error very big, and in order to This experimental error is avoided, pipette tips can be against on the inner wall of cell plate hole by general operation process, and it is slowly added to sample liquid, with Reduce influence to attached cell, but operate in this way it is time-consuming and laborious, and still can not stability contorting sample liquids flow velocity and Cell monolayer is destroyed, while being also easy pollution pipette tips, pipette tips is needed to frequently replace in such operating process, operates very complicated.
Utility model content
It, can be in order to be loaded in view of the above-mentioned problems, the utility model provides a kind of tissue culture plate, and it can be effective The flow velocity of control sample-adding sample liquid, makes the cell monolayer in tissue culture plate be not easy to be destroyed, will not pollute pipette tips, can be substantially Improve the operating efficiency of experimentation addition sample liquid.
The utility model is achieved through the following technical solutions.
Tissue culture plate, it includes tissue culture plate ontology, the cultivation plate hole that is arranged on tissue culture plate ontology, special Sign is that it further includes the pipette tips card that the intrinsic diversion trench of tissue culture plate is arranged in, is provided with tissue culture plate body top surface Hole;The diversion trench has the ramped bottom surface of a cultivation plate hole for making to be loaded liquid flow direction;The diversion trench is located at inclination bottom One end of face lower position is connected to the side wall of cultivation plate hole, and the diversion trench is located at one end and the rifle of ramped bottom surface higher position Head card hole connection.
As optimal technical scheme, the surface of the ramped bottom surface is frosting structure.
As optimal technical scheme, the pipette tips card hole is inverted circular cone rod structure.
As optimal technical scheme, one end that the ramped bottom surface is connected to cultivation plate hole side wall is globoidal structure.
The utility model beneficial effect is: the utility model structure is simple, easy to operate, can be convenient for adding sample repeatedly Liquid without polluting pipette tips, while can control the flow velocity of sample liquid, and the cell monolayer that can effectively avoid in cultivation plate hole is destroyed.
Detailed description of the invention
Fig. 1 is the top view of the utility model tissue culture plate;
Fig. 2 is the partial enlarged view of section at A-A in Fig. 1;
Fig. 3 is the partial enlarged view of section at B-B in Fig. 1;
Fig. 4 is state diagram when the utility model is loaded using liquid-transfering gun;
The meaning respectively identified in above-mentioned figure are as follows: 1- tissue culture plate ontology, 2- pipette tips card hole, 3- cultivation plate hole, 4- water conservancy diversion Slot, 5- pipette tips, 6- ramped bottom surface.
Specific embodiment
The utility model is described further in the form of specific embodiment with reference to the accompanying drawing, it is pointed out that with Lower embodiment is only the indicative explaination that the form to enumerate does the utility model, but the protection scope of the utility model It is not limited to that, all those skilled in the art equivalent are replaced with the spirit of the utility model to what the utility model was done It changes and each falls within the protection scope of the utility model.
Embodiment
Tissue culture plate please refers to Fig. 1, Fig. 2, Fig. 3, it includes tissue culture plate ontology 1, is arranged in tissue culture plate sheet Cultivation plate hole 3 on body 1, is provided with 1 top surface of tissue culture plate ontology at the diversion trench 4 being arranged in tissue culture plate ontology 1 Pipette tips card hole 2;Fig. 2, Fig. 3 are please referred to, diversion trench 4 has the ramped bottom surface 6 of a cultivation plate hole 3 for making to be loaded liquid flow direction, One end that diversion trench 4 is located at 6 lower position of ramped bottom surface is connected to the side wall of cultivation plate hole 3, and diversion trench 4 is located at ramped bottom surface 6 One end of higher position is connected to pipette tips card hole 2;When the utility model is loaded using liquid-transfering gun, referring to Fig. 4, by liquid-transfering gun Pipette tips 5 are directed at pipette tips card hole 2, are caught in pipette tips 5 slowly in pipette tips card hole 2, later slowly infuse the sample-adding liquid in pipette tips 5 Enter, the sample-adding liquid in such pipette tips 5 passes sequentially through the ramped bottom surface 6 of diversion trench 4, the side wall of cultivation plate hole 3 flows slowly into training It supports in plate hole 3, after the sample-adding liquid in pipette tips 5 has been infused, slowly takes out pipette tips 5 from pipette tips card hole 2, repeat aforesaid operations Carry out the addition that next cultivation plate hole 3 is loaded liquid.
It is further decreased to make to be loaded its flow velocity when liquid flows through ramped bottom surface 6, further, referring to Fig. 3, inclination The surface of bottom surface 6 is frosting structure.
Further, Fig. 2, Fig. 3 are please referred to, pipette tips card hole 2 is inverted circular cone rod structure, the rifle of such circular cone rod structure Head card hole 2 is matched with the shape of the pipette tips 5 of liquid-transfering gun, and pipette tips card hole 2 can be caught in convenient for pipette tips 5 or is extracted from pipette tips card hole 2, and The scraping of pipette tips 5 Yu pipette tips card hole 2 can be reduced.
Sample-adding liquid directly flows into culture hole 3 from ramped bottom surface 6 in order to prevent, and is not passed through the side wall of cultivation plate hole 3, Further, referring to Fig. 2, one end that ramped bottom surface 6 is connected to 3 side wall of cultivation plate hole is globoidal structure.

Claims (4)

1. tissue culture plate, it includes the cultivation plate hole of tissue culture plate ontology (1), setting on tissue culture plate ontology (1) (3), it is characterised in that it further includes the diversion trench (4) being arranged in tissue culture plate ontology (1), is provided with tissue culture plate sheet The pipette tips card hole (2) of body (1) top surface;The diversion trench (4) has the inclination of a cultivation plate hole (3) for making to be loaded liquid flow direction Bottom surface (6);One end that the diversion trench (4) is located at ramped bottom surface (6) lower position is connected to the side wall of cultivation plate hole (3), institute It states diversion trench (4) and is located at one end of ramped bottom surface (6) higher position and be connected to pipette tips card hole (2).
2. tissue culture plate as described in claim 1, it is characterised in that the surface of the ramped bottom surface (6) is frosting knot Structure.
3. tissue culture plate as described in claim 1, it is characterised in that the pipette tips card hole (2) is inverted circular cone column knot Structure.
4. tissue culture plate as described in claim 1, it is characterised in that the ramped bottom surface (6) and cultivation plate hole (3) side wall One end of connection is globoidal structure.
CN201821626194.6U 2018-10-02 2018-10-02 Tissue culture plate Expired - Fee Related CN209081911U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201821626194.6U CN209081911U (en) 2018-10-02 2018-10-02 Tissue culture plate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201821626194.6U CN209081911U (en) 2018-10-02 2018-10-02 Tissue culture plate

Publications (1)

Publication Number Publication Date
CN209081911U true CN209081911U (en) 2019-07-09

Family

ID=67118558

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201821626194.6U Expired - Fee Related CN209081911U (en) 2018-10-02 2018-10-02 Tissue culture plate

Country Status (1)

Country Link
CN (1) CN209081911U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112442440A (en) * 2019-08-30 2021-03-05 台达电子工业股份有限公司 Biological detection cassette and method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112442440A (en) * 2019-08-30 2021-03-05 台达电子工业股份有限公司 Biological detection cassette and method

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GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20190709

Termination date: 20201002