CN210340861U

CN210340861U - Transposase fragmentation reaction system accommodating device

Info

Publication number: CN210340861U
Application number: CN201921203380.3U
Authority: CN
Inventors: 张惠丹; 戴敬; 杨晟; 黎晗; 姜坤廷
Original assignee: Suzhou Yimei Biotechnology Co ltd
Current assignee: Suzhou Yimei Biotechnology Co ltd
Priority date: 2019-07-29
Filing date: 2019-07-29
Publication date: 2020-04-17
Anticipated expiration: 2029-07-29

Abstract

The utility model discloses a transposase fragmentation reaction system holding device, include: the low-temperature container and the normal-temperature container are detachably connected; the low-temperature container comprises a first end cover, a refrigeration interlayer and a first reagent area, the normal-temperature container comprises a second end cover, a first layer area and a second layer area, and the first layer area is positioned above the second layer area; the first layer area comprises a second reagent area and a powder area, the second reagent area and the powder area are separated through a partition plate, the second layer area is of a drawable drawer type structure, and a handle is arranged outside the second layer area. A transposase fragmentation reaction system accommodation device low temperature container and the removable use of normal atmospheric temperature container and combination use, provide the required whole reagent of contrast inspection between transposon structure step, DNA enrichment, purification, sorting step and step, the used reagent of experiment can classified storage, concentrate the use, improved experimental efficiency greatly.

Description

Transposase fragmentation reaction system accommodating device

Technical Field

The utility model belongs to the technical field of molecular biology equipment, especially, relate to a transposase fragmentation reaction system holding device.

Background

The existing commercial transposase method library building kit is simple in packaging, does not have the effect of keeping the reagent low temperature and stable, needs to artificially provide an environment at-20 ℃ during transportation and storage, has high requirements on transportation cold chain, needs experimenters to prepare a low-temperature environment by themselves during use, and needs repeated freezing and thawing of the reagent. The construction of the DNA library by the transposase method involves a plurality of reagent types and complicated steps, and reagents are frequently repeatedly taken and stored between a refrigerator and an experiment table in the experiment process, so that the experiment process is low in efficiency and prone to errors.

Meanwhile, in the experimental process of constructing the DNA library by the transposase method, reagents and products required in the transposon construction step and the DNA enrichment step need to be stored at the temperature of-20 ℃ and used on ice, reagents required in the DNA purification and sorting step need to be stored and used at normal temperature, and no device capable of being concentrated and separated exists at present, so that the system used in the experiment can be concentrated and simplified.

SUMMERY OF THE UTILITY MODEL

To the above problem, the utility model provides a transposase fragmentation reaction system accommodating device of centralization, self-carrying low temperature environment can dismantle, the dress of equipment for the used reagent of experiment can classified storage, concentrate the use, improves experimental efficiency.

In order to achieve the above object, the utility model adopts the following technical scheme:

a transposase fragmentation reaction system containment device comprising: the low-temperature container and the normal-temperature container are detachably connected; the low-temperature container comprises a first end cover, a refrigeration interlayer and a first reagent area, the normal-temperature container comprises a second end cover, a first layer area and a second layer area, and the first layer area is positioned above the second layer area; the first layer area comprises a second reagent area and a powder area, the second reagent area and the powder area are separated through a partition plate, the second layer area is of a drawable drawer type structure, and a handle is arranged outside the second layer area.

Preferably, the refrigeration interlayer is arranged on the periphery and the bottom of the first reagent zone.

More preferably, the refrigerating interlayer is filled with a cellulose gel refrigerant.

The optimization effect brought by the optimization scheme is that the cold-keeping agent can provide a low-temperature environment for 7 hours, and the continuous operation of the whole experiment process is met.

Preferably, the first reagent zone and the second reagent zone are provided with a plurality of test tube holes for accommodating reagent tubes.

More preferably, the first reagent region is divided into three regions by providing the test tube holes with different colors, the first region stores the Tn5 enzyme, the linker sequence and the buffer solution, the second region stores the amplification enzyme, the amplification buffer solution and the amplification primers, and the third region stores the product of the first region, the sample retention after DNA conversion and the control DNA.

The optimization effect brought by the optimization scheme is that the concentrated and separated management and placement of the medicament are realized, and the experiment efficiency is improved.

More preferably, the second reagent region is used for storing magnetic beads, a stop solution, absolute ethanol, sterile water and a loading buffer solution.

Preferably, the powder area is used for storing agarose powder.

Preferably, the first end cover and the second end cover are both opened and closed through magnet attraction.

Preferably, the second layer region comprises a double-layer frame structure which is a magnetic frame.

More preferably, the low-temperature container and the normal-temperature container are connected through a mortise and tenon structure.

The optimized effect brought by the optimized scheme is that the transposase fragmentation reaction system accommodating device can be detachably connected in a partitioned mode, and the storage and the centralized management during experiments are facilitated.

Compared with the prior art, the beneficial effects of the utility model are that:

1. a transposase fragmentation reaction system storage device from taking cold-proof agent, can keep the stability of reagent in storage, transportation and use.

2. A transposase fragmentation reaction system accommodating device possess two containers of low temperature container and normal atmospheric temperature container, can avoid in the experimentation reagent to fetch repeatedly between refrigerator and laboratory bench, lead to the experimentation inefficiency and easily make mistakes.

3. A transposase fragmentation reaction system accommodation device low temperature container and normal atmospheric temperature container but split use and use, the split is deposited during the storage, can make up during the use, provides the required whole reagents of contrast inspection between transposon structure step, DNA enrichment, purification, sorting step and step, the used reagent of experiment can classified storage, centralized use, the researcher only need additionally prepare the PCR appearance when using can, improved experimental efficiency greatly.

Drawings

FIG. 1 is a schematic view of a low temperature container of a transposase fragmentation reaction system container according to the present invention.

FIG. 2 is a schematic view of a normal temperature container of the device for containing transposase fragmentation reaction system of the present invention.

FIG. 3 is a schematic structural view of a transposase fragmentation reaction system containing device of the present invention after the low temperature container and the normal temperature container are connected.

The device comprises a low-temperature container 1, a low-temperature container 11, a first end cover 12, a refrigerating interlayer 13 and a first reagent area; 2. a normal temperature container, 21, a second end cover, 22, a first layer area, 221, a second reagent area, 222, a powder area, 223, a partition plate, 23, a second layer area, 231, a double-layer frame structure (magnetic frame), 232, an EP pipe hole, 233, a PCR pipe hole, 234 and a handle; 3. connecting tenons; 4. connecting the mortises; 5. a magnet; 6. test tube hole.

Detailed Description

For a better understanding of the present invention, the contents of the present invention will be further clarified below with reference to the accompanying drawings and examples, but the present invention is not limited to the following examples.

Examples

It should be noted that the terms "first," "second," and the like in the description and claims of the present invention and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged under appropriate circumstances for purposes of describing the embodiments of the invention herein. Further, the orientations and positional relationships indicated by the terms "upper", "lower", "top", "bottom", "inner", "outer", and the like are all based on the orientations and positional relationships shown in the drawings, and the objects are only for convenience of description and simplification of the description, and do not indicate or imply that the indicated components must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

A transposase fragmentation reaction system containment device comprising: the low-temperature container 1 and the normal-temperature container 2 are detachably connected with each other. The low-temperature container 1 comprises a first end cover 11, a refrigeration interlayer 12 and a first reagent area 13, wherein the refrigeration interlayer 12 is arranged on the periphery and the bottom of the first reagent area 13. In this embodiment, the refrigeration interlayer 12 is filled with a cellulose gel refrigerant, which can provide a low temperature environment for 7 hours, and the continuous operation of the whole experiment process is satisfied. The first reagent area 13 is provided with a plurality of test tube holes 6 for accommodating reagent tubes, the first reagent area 13 is divided into three areas through the test tube holes 6 with different colors, Tn5 enzyme, a connector sequence and buffer solution are stored in the first area, amplification enzyme, amplification buffer solution and amplification primers are stored in the second area, and products in the first area, sample retention after DNA conversion and control DNA are stored in the third area. Test tube reagents with different purposes are placed in corresponding color areas, and partitioned management is achieved, so that experiment efficiency is improved.

The ambient temperature container 2 includes a second end cap 21, a first layer region 22, and a second layer region 23, and the first layer region 22 is located above the second layer region 23. The first layer region 22 includes a second reagent region 221 and a powder region 223, and the first layer region 22 is divided into the second reagent region 221 and the powder region 223 by a partition plate 223. The second reagent zone 221 is also provided with a plurality of test tube wells 6 for accommodating reagent tubes, the second reagent zone 221 is used for storing magnetic beads, stop solution, absolute ethyl alcohol, sterile water and sample buffer solution, and test tube reagents with different purposes are placed in corresponding areas. The powder section 223 is used to store agarose powder. The agarose powder can be packaged individually for a specific weight and can be used directly during the experiment. The second layer region 23 includes a double-layer frame structure 231, the double-layer frame structure 231 is a magnetic frame, the double-layer frame structure 231 is provided with an EP tube hole 232 and a PCR tube hole 233, the EP tube hole 232 is suitable for an EP tube of 1.5/2ml, and the PCR tube hole 233 is suitable for a PCR tube of 200 μ L. The second layer 23 is of a drawer-type construction and is externally provided with a handle 234.

The first end cover 11 and the second end cover 21 are both opened and closed through the attraction of the magnets 5, and the paired magnets 5 can be arranged on the outer surfaces of the end covers and the corresponding containers. The low-temperature container 1 and the normal-temperature container 2 are connected through a mortise and tenon structure, in the embodiment, the low-temperature container 1 is provided with a connecting tenon 3, and the normal-temperature container 2 is provided with a connecting mortise 4.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A transposase fragmentation reaction system accommodating device is characterized by comprising: the low-temperature container and the normal-temperature container are detachably connected; the low-temperature container comprises a first end cover, a refrigeration interlayer and a first reagent area, the normal-temperature container comprises a second end cover, a first layer area and a second layer area, and the first layer area is positioned above the second layer area; the first layer area comprises a second reagent area and a powder area, the second reagent area and the powder area are separated through a partition plate, the second layer area is of a drawable drawer type structure, and a handle is arranged outside the second layer area.

2. The apparatus as claimed in claim 1, wherein the refrigerating layer is disposed around and at the bottom of the first reagent region.

3. The apparatus as claimed in claim 2, wherein the refrigerating interlayer is filled with a cellulose gel refrigerant.

4. The apparatus as claimed in claim 1, wherein the first reagent region and the second reagent region each have a plurality of wells for receiving reagent tubes.

5. The apparatus as claimed in claim 4, wherein the first reagent region is divided into three regions by the test tube holes with different colors, the first region stores Tn5 enzyme, linker sequence and buffer, the second region stores amplification enzyme, amplification buffer and amplification primers, and the third region stores products of the first region, DNA-converted sample residues and control DNA.

6. The transposase fragmentation reaction system containment device of claim 4, wherein the second reagent region is configured to store magnetic beads, stop solution, absolute ethanol, sterile water, and loading buffer.

7. The transposase fragmentation reaction system containment device of claim 1, wherein the powder region is configured to hold agarose powder.

8. The apparatus as claimed in claim 1, wherein the first and second end caps are opened and closed by magnetic attraction.

9. The transposase fragmentation reaction system containment device of claim 1, wherein the second layer region comprises a double-layered framework structure, and the double-layered framework structure is a magnetic framework.

10. The apparatus as claimed in any of claims 1 to 9, wherein the low temperature vessel and the normal temperature vessel are connected by a mortise and tenon structure.