SG11201910019PA - Microorganisms and methods for producing cannabinoids and cannabinoid derivatives - Google Patents

Microorganisms and methods for producing cannabinoids and cannabinoid derivatives

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Publication number
SG11201910019PA
SG11201910019PA SG11201910019PA SG11201910019PA SG 11201910019P A SG11201910019P A SG 11201910019PA SG 11201910019P A SG11201910019P A SG 11201910019PA SG 11201910019P A SG11201910019P A SG 11201910019PA
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Singapore
Prior art keywords
california
cannabinoid
ave
suite
international
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Jay D Keasling
Leo D'espaux
Jeff Wong
Xiaozhou Luo
Michael Reiter
Charles Denby
Anna Lechner
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Univ California
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Publication of SG11201910019PA publication Critical patent/SG11201910019PA/en

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C63/00—Compounds having carboxyl groups bound to a carbon atoms of six-membered aromatic rings
    • C07C63/04—Monocyclic monocarboxylic acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
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    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01102—Geranyl-pyrophosphate—olivetolic acid geranyltransferase (2.5.1.102)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01088—Hydroxymethylglutaryl-CoA reductase (1.1.1.88)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01086—Fatty-acyl-CoA synthase (2.3.1.86)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01206—3,5,7-Trioxododecanoyl-CoA synthase (2.3.1.206)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/0301—Hydroxymethylglutaryl-CoA synthase (2.3.3.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01036—Mevalonate kinase (2.7.1.36)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04002—Phosphomevalonate kinase (2.7.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01033—Diphosphomevalonate decarboxylase (4.1.1.33), i.e. mevalonate-pyrophosphate decarboxylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/03—Intramolecular oxidoreductases (5.3) transposing C=C bonds (5.3.3)
    • C12Y503/03002—Isopentenyl-diphosphate DELTA-isomerase (5.3.3.2)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01003—Long-chain-fatty-acid-CoA ligase (6.2.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 01 November 2018 (01.11.2018) omits I E H 01010 MODE 011 (10) International Publication Number WO 2018/200888 Al WIPO I PCT (51) International Patent Classification: C12N 9/10 (2006.01) Cl 2P 7/22 (2006.01) C12N 15/52 (2006.01) Cl 2P 17/06 (2006.01) (21) International Application Number: PCT/US2018/029668 (22) International Filing Date: MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) W O 20 18/ 200888 Al 27 April 2018 (27.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/491,114 27 April 2017 (27.04.2017) US 62/569,532 07 October 2017 (07.10.2017) US (71) Applicant: REGENTS OF THE UNIVERSITY OF CALIFORNIA [US/US]; 1111 Franklin Street, Oakland, California 94607 (US). (72) Inventors: KEASLING, Jay D.; 2150 Shattuck Ave., Suite 510, Berkeley, California 94704 (US). D'ESPAUX, Leo; 2150 Shattuck Ave., Suite 510, Berkeley, California 94704 (US). WANG, Jeff; 2150 Shattuck Ave., Suite 510, Berke- ley, California 94704 (US). LUO, Xiaozhou; 2150 Shat- tuck Ave., Suite 510, Berkeley, California 94704 (US). REITER, Michael; 2150 Shattuck Ave., Suite 510, Berke- ley, California 94704 (US). DENBY, Charles; 2150 Shat- tuck Ave., Suite 510, Berkeley, California 94704 (US). LECHNER, Anna; 2150 Shattuck Ave., Suite 510, Berke- ley, California 94704 (US). (74) Agent: OSMAN, Richard Aron; 530 Lawrence Expy #332, Sunnyvale, California 94085 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (54) Title: MICROORGANISMS AND METHODS FOR PRODUCING CANNABINOIDS AND CANNABINOID DERIVATIVES (57) : The present disclosure provides genetically modified host cells that produce a cannabinoid, a cannabinoid derivative, a cannabinoid precursor, or a cannabinoid precursor derivative. The present disclosure provides methods of synthesizing a cannabinoid, a cannabinoid derivative, a cannabinoid precursor, or a cannabinoid precursor derivative.
SG11201910019P 2017-04-27 2018-04-27 Microorganisms and methods for producing cannabinoids and cannabinoid derivatives SG11201910019PA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762491114P 2017-04-27 2017-04-27
US201762569532P 2017-10-07 2017-10-07
PCT/US2018/029668 WO2018200888A1 (en) 2017-04-27 2018-04-27 Microorganisms and methods for producing cannabinoids and cannabinoid derivatives

Publications (1)

Publication Number Publication Date
SG11201910019PA true SG11201910019PA (en) 2019-11-28

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SG11201910019P SG11201910019PA (en) 2017-04-27 2018-04-27 Microorganisms and methods for producing cannabinoids and cannabinoid derivatives

Country Status (11)

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US (4) US10563211B2 (en)
EP (2) EP3998336A1 (en)
JP (1) JP7198555B2 (en)
CN (1) CN110914416B (en)
AU (1) AU2018256863B2 (en)
BR (1) BR112019022500A2 (en)
CA (1) CA3061718A1 (en)
ES (1) ES2898272T3 (en)
IL (1) IL270202B1 (en)
SG (1) SG11201910019PA (en)
WO (1) WO2018200888A1 (en)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11180781B2 (en) * 2016-08-21 2021-11-23 Insectergy, Llc Biosynthetic cannabinoid production methods
US10239808B1 (en) 2016-12-07 2019-03-26 Canopy Holdings, LLC Cannabis extracts
WO2018148849A1 (en) 2017-02-17 2018-08-23 Hyasynth Biologicals Inc. Method and cell line for production of polyketides in yeast
SG11201910019PA (en) * 2017-04-27 2019-11-28 Univ California Microorganisms and methods for producing cannabinoids and cannabinoid derivatives
US10837031B2 (en) 2017-05-10 2020-11-17 Baymedica, Inc. Recombinant production systems for prenylated polyketides of the cannabinoid family
EP3652327A4 (en) * 2017-07-12 2021-04-21 Biomedican, Inc. Production of cannabinoids in yeast
US11981938B2 (en) * 2017-10-05 2024-05-14 Eleszto Genetika, Inc. Microorganisms and methods for the fermentation of cannabinoids
US11202771B2 (en) 2018-01-31 2021-12-21 Treehouse Biotech, Inc. Hemp powder
CA3079760C (en) * 2018-04-30 2021-11-23 Algae-C Inc. Engineered microorganism for the production of cannabinoid biosynthetic pathway products
US10801049B2 (en) 2018-06-14 2020-10-13 Syntiva Therapeutics, Inc. Genetically engineered microorganisms and processes for the production of cannabinoids from a carbon source precursor
CA3107544A1 (en) 2018-08-01 2020-02-06 The Regents Of The University Of California Biosynthetic platform for the production of cannabinoids and other prenylated compounds
WO2020060948A1 (en) 2018-09-17 2020-03-26 Levadura Biotechnology, Inc. Production of cannabinoids in yeast using a fatty acid feedstock
WO2020069214A2 (en) * 2018-09-26 2020-04-02 Demetrix, Inc. Optimized expression systems for producing cannabinoid synthase polypeptides, cannabinoids, and cannabinoid derivatives
CA3119729A1 (en) 2018-10-10 2020-04-16 Treehouse Biotech, Inc. Synthesis of cannabigerol
CN113227353A (en) * 2018-11-14 2021-08-06 马努斯生物合成股份有限公司 Microbial cells and methods for cannabinoid production
WO2020112647A1 (en) * 2018-11-27 2020-06-04 Khona Scientific Llc Bidirectional multi-enzymatic scaffolds for biosynthesizing cannabinoids
EP3917642A4 (en) * 2019-01-30 2023-04-05 Genomatica, Inc. Recovery, decarboxylation, and purification of cannabinoids from engineered cell cultures
US20220127649A1 (en) * 2019-01-30 2022-04-28 Genomatica, Inc. Engineered cells for improved production of cannabinoids
EP3921434A4 (en) * 2019-02-10 2022-11-30 Dyadic International (USA), Inc. Production of cannabinoids in filamentous fungi
WO2020169221A1 (en) * 2019-02-20 2020-08-27 Synbionik Gmbh Production of plant-based active substances (e.g. cannabinoids) by recombinant microorganisms
WO2020176547A1 (en) 2019-02-25 2020-09-03 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
US20220243236A1 (en) * 2019-03-01 2022-08-04 The Regents Of The University Of California Production of cannabinoids using genetically engineered photosynthetic microorganisms
MX2021011185A (en) * 2019-03-15 2021-10-22 Amyris Inc Microbial production of compounds.
US20220186267A1 (en) * 2019-03-27 2022-06-16 Rynetech Bio, Inc. Biosynthetic Cannabidiol Production In Engineered Microorganisms
WO2020208411A2 (en) * 2019-04-11 2020-10-15 Eleszto Genetika, Inc. Microorganisms and methods for the fermentation of cannabinoids
WO2020210810A1 (en) * 2019-04-12 2020-10-15 Renew Biopharma, Inc. Compositions and methods for using genetically modified enzymes
BR112021023218A2 (en) * 2019-05-22 2022-02-08 Demetrix Inc Optimized cannabinoid synthase polypeptides
CA3142619A1 (en) * 2019-06-06 2020-12-10 Genomatica, Inc. Olivetolic acid cyclase variants and methods for their use
US20220298522A1 (en) * 2019-07-30 2022-09-22 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization Methods of controlling cannabinoid synthesis in plants or cells and plants and cells produced thereby
WO2021041572A1 (en) * 2019-08-27 2021-03-04 Natural Extraction Systems, LLC Compositions comprising decarboxylated cannabinoids
WO2021042057A1 (en) * 2019-08-30 2021-03-04 Lygos, Inc. Systems and methods for preparing cannabinoids and derivatives
WO2021035359A1 (en) * 2019-08-30 2021-03-04 Exponential Genomics Canada Inc. Production of gpp and cbga in a methylotrophic yeast strain
WO2021055597A1 (en) * 2019-09-18 2021-03-25 Demetrix, Inc. Optimized tetrahydrocannabinolic acid (thca) synthase polypeptides
CN114729386A (en) * 2019-10-01 2022-07-08 杭州恩和生物科技有限公司 Enzymes for cannabinoid synthesis and methods of making and using same
JP2022552952A (en) * 2019-10-11 2022-12-21 ナショナル ユニヴァーシティ オブ シンガポール Biosynthesis of cannabinoid precursors using a novel aromatic prenyltransferase
JP2022552953A (en) * 2019-10-11 2022-12-21 ナショナル ユニヴァーシティ オブ シンガポール Sustainable production of cannabinoids from simple precursor raw materials using Saccharomyces cerevisiae
EP4041874A4 (en) * 2019-10-11 2024-02-21 National University of Singapore Biosynthesis of cannabinoids from cannabigerolic acid using novel cannabinoid synthases
CN110669713A (en) * 2019-10-18 2020-01-10 中国科学院青岛生物能源与过程研究所 Genetically engineered bacterium for synthesizing D-limonene and construction method and application thereof
WO2021081648A1 (en) * 2019-10-29 2021-05-06 Algae-C Inc. Engineered microorganism for the production of cannabinoid biosynthetic pathway products
EP4087933A1 (en) * 2020-01-10 2022-11-16 Barrit Sarl Production of bioactive bibenzylic acid or derivatives thereof by genetically modified microbial hosts
BR112022013503A2 (en) * 2020-01-10 2022-09-13 Univ California BIOSYNTHETIC PLATFORM FOR THE PRODUCTION OF OLIVETOLIC ACID AND OLIVETOLIC ACID ANALOGS
JP2023511109A (en) * 2020-01-20 2023-03-16 ベイメディカ インコーポレイテッド Genetically modified yeast for the production of cannabigerolic acid, cannabichromenic acid and related cannabinoids
CN113999870B (en) * 2020-02-26 2024-02-20 森瑞斯生物科技(深圳)有限公司 Recombinant saccharomyces cerevisiae for expressing CBDAS, and construction method and application thereof
CA3174592A1 (en) * 2020-03-03 2021-09-10 Ido Margalit Odorless cannabis plant
WO2021183448A1 (en) 2020-03-09 2021-09-16 Demetrix, Inc. Optimized olivetolic acid cyclase polypeptides
WO2021195517A2 (en) 2020-03-27 2021-09-30 Willow Biosciences, Inc. Compositions and methods for recombinant biosynthesis of cannabinoids
EP4142711A1 (en) 2020-04-29 2023-03-08 Willow Biosciences Inc. Compositions and methods for enhancing recombinant biosynthesis of cannabinoids
EP4146809A1 (en) * 2020-05-08 2023-03-15 Lygos, Inc. Large scale production of olivetol, olivetolic acid and other alkyl resorcinols by fermentation
BR112023002818A2 (en) * 2020-08-19 2023-04-25 Amyris Inc MICROBIAL PRODUCTION OF CANNABINOIDS
CN112063647B (en) * 2020-09-17 2023-05-02 云南农业大学 Construction method of saccharomyces cerevisiae recombinant Cuol01, saccharomyces cerevisiae recombinant Cuol02 and application
CN112280699B (en) * 2020-09-28 2022-06-21 嘉兴欣贝莱生物科技有限公司 Method for producing amyl dihydroxy benzoic acid
WO2022081615A1 (en) * 2020-10-13 2022-04-21 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
CN112410235A (en) * 2020-11-23 2021-02-26 森瑞斯生物科技(深圳)有限公司 Saccharomyces cerevisiae strain for high yield of cannabigerol and construction method and application thereof
US11884620B2 (en) 2020-12-11 2024-01-30 The Regents Of The University Of California Use of polyamines in the pretreatment of biomass
CA3201757A1 (en) 2020-12-11 2022-06-16 Mathias Schuetz Recombinant acyl activating enzyme (aae) genes for enhanced biosynthesis of cannabinoids and cannabinoid precursors
CN112795495B (en) * 2020-12-14 2021-10-26 大连理工大学 Method for producing heterologous cannabichromene by using saccharomyces cerevisiae
CN113234845B (en) * 2021-03-12 2023-03-24 内蒙古农业大学 SNP molecular marker primer and marking method for identifying main-cultivated jujube variety in inner Mongolia region
WO2022204007A2 (en) * 2021-03-22 2022-09-29 Willow Biosciences, Inc. Recombinant polypeptides for enhanced biosynthesis of cannabinoids
WO2022241298A2 (en) * 2021-05-14 2022-11-17 Cellibre, Inc. Engineered cells, enzymes, and methods for producing cannabinoids
CN113278597B (en) * 2021-05-26 2023-04-21 重庆大学 Novel short side chain fatty acid CoA ligase and application thereof in preparation of patchoulenone
BR112023025334A2 (en) * 2021-06-04 2024-02-27 Amyris Inc CANNABINOID PURIFICATION METHODS
CN113528365B (en) * 2021-07-13 2023-04-07 浙江寿仙谷医药股份有限公司 Recombinant saccharomyces cerevisiae for producing cannabidiol, construction method and application thereof
CA3227215A1 (en) 2021-07-30 2023-02-02 Trish Choudhary Recombinant prenyltransferase polypeptides engineered for enhanced biosynthesis of cannabinoids
WO2023023621A1 (en) 2021-08-19 2023-02-23 Willow Biosciences, Inc. Recombinant olivetolic acid cyclase polypeptides engineered for enhanced biosynthesis of cannabinoids
CN113502254B (en) * 2021-09-10 2022-01-07 北京蓝晶微生物科技有限公司 Olive alcohol synthetase variants and engineered microorganisms expressing same
CN113502255B (en) * 2021-09-10 2022-01-28 北京蓝晶微生物科技有限公司 Engineered microorganisms for the production of olivetol and olivetol
WO2023069921A1 (en) 2021-10-19 2023-04-27 Epimeron Usa, Inc. Recombinant thca synthase polypeptides engineered for enhanced biosynthesis of cannabinoids
CN114214339A (en) * 2021-12-08 2022-03-22 福建农林大学 Hemp THCSAS2 gene, terpene phenolic acid oxidative cyclase as coded product thereof and application of terpene phenolic acid oxidative cyclase
CN114591923B (en) * 2022-05-10 2022-08-30 森瑞斯生物科技(深圳)有限公司 Cannabidiol synthetase mutant and construction method and application thereof
WO2023220728A2 (en) * 2022-05-12 2023-11-16 Manus Bio Inc. Enzymes, cells, and methods for producing cis-3 hexenol
WO2024042486A1 (en) 2022-08-26 2024-02-29 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for the production of polyurethanes
WO2024042405A1 (en) 2022-08-26 2024-02-29 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for the synthesis of bio-based polymers
WO2024116153A1 (en) 2022-12-02 2024-06-06 Amyris Bio Products Portugal, Unipessoal, Ltda. Compositions and methods for using previously cultured cells
CN116024111A (en) * 2022-12-03 2023-04-28 中国科学院深圳先进技术研究院 Microbial cell for producing cannabigerol, and construction method and application thereof
CN116622784B (en) * 2023-02-14 2024-03-01 黑龙江八一农垦大学 Application of cannabidiol synthase
CN116574700B (en) * 2023-05-12 2023-11-14 黑龙江八一农垦大学 Cannabidiol synthetase mutant and application thereof

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5576198A (en) 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5545817A (en) 1994-03-11 1996-08-13 Calgene, Inc. Enhanced expression in a plant plastid
US5545818A (en) 1994-03-11 1996-08-13 Calgene Inc. Expression of Bacillus thuringiensis cry proteins in plant plastids
WO1996017951A2 (en) 1994-12-09 1996-06-13 Rpms Technology Limited Identification of genes responsible for in vivo survival of microorganisms
US6900012B1 (en) 1997-06-03 2005-05-31 The University Of Chicago Plant artificial chromosome compositions and methods
US6080849A (en) 1997-09-10 2000-06-27 Vion Pharmaceuticals, Inc. Genetically modified tumor-targeted bacteria with reduced virulence
WO2002010398A2 (en) 2000-07-31 2002-02-07 Hahn Frederick M Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
GB0105924D0 (en) 2001-03-09 2001-04-25 Microscience Ltd Promoter
US6733996B2 (en) 2002-08-26 2004-05-11 Trustees Of Dartmouth College Methods for regulating gene expression using light
CA2501574C (en) 2002-10-04 2013-12-03 E. I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
MX284139B (en) 2006-05-26 2011-02-18 Amyris Biotechnologies Inc Production of isoprenoids.
SG162265A1 (en) 2007-12-13 2010-07-29 Danisco Us Inc Compositions and methods for producing isoprene
SG165884A1 (en) 2008-04-23 2010-11-29 Goodyear Tire & Rubber Isoprene synthase variants for improved microbial production of isoprene
CA2729801A1 (en) 2008-07-02 2010-01-07 Danisco Us Inc. Compositions and methods for producing isoprene free of c5 hydrocarbons under decoupling conditions and/or safe operating ranges
WO2011017798A1 (en) 2009-08-12 2011-02-17 National Research Council Of Canada Aromatic prenyltransferase from cannabis
CA3130705A1 (en) 2010-04-15 2011-10-20 National Research Council Of Canada Genes and proteins for aromatic polyketide synthesis
JP6177774B2 (en) 2011-07-13 2017-08-16 ナショナル、リサーチ、カウンシル、オブ、カナダＮａｔｉｏｎａｌ Ｒｅｓｅａｒｃｈ Ｃｏｕｎｃｉｌ ｏｆ Ｃａｎａｄａ Genes and proteins for alkanoyl-COA synthesis
BR112015020332A2 (en) 2013-02-28 2017-07-18 Full Spectrum Laboratories Ltd chemical engineering processes and apparatus for the synthesis of compounds
EP2968259B1 (en) 2013-03-14 2022-09-14 SC Laboratories Inc. Bioactive concentrates and uses thereof
DK3553177T3 (en) 2013-03-15 2021-02-15 Univ Leland Stanford Junior BENZYLISOQUINOLINAL KALOIDS (BIA) PRODUCING MICROBS, AND METHODS OF MANUFACTURE AND USE
WO2015196275A1 (en) 2014-06-27 2015-12-30 National Research Council Of Canada (Nrc) Cannabichromenic acid synthase from cannabis sativa
WO2016010827A1 (en) 2014-07-14 2016-01-21 Librede Inc. Production of cannabinoids in yeast
AU2015308136B2 (en) 2014-08-25 2020-07-09 Teewinot Technologies Limited Apparatus and methods for the simultaneous production of cannabinoid compounds
EP3250200A4 (en) 2015-01-31 2018-09-19 Constance Therapeutics, Inc. Methods for preparation of cannabis oil extracts and compositions
EP3067058A1 (en) * 2015-03-13 2016-09-14 Farmagens Health Care Srl Biological composition based on engineered lactobacillus paracasei subsp. paracasei f19 for the biosynthesis of cannabinoids
US20160298151A1 (en) 2015-04-09 2016-10-13 Sher Ali Butt Novel Method for the cheap, efficient, and effective production of pharmaceutical and therapeutic api's intermediates, and final products
WO2017139496A1 (en) 2016-02-09 2017-08-17 Cevolva Biotech, Inc. Microbial engineering for the production of cannabinoids and cannabinoid precursors
WO2018148849A1 (en) 2017-02-17 2018-08-23 Hyasynth Biologicals Inc. Method and cell line for production of polyketides in yeast
SG11201910019PA (en) * 2017-04-27 2019-11-28 Univ California Microorganisms and methods for producing cannabinoids and cannabinoid derivatives
EP3652327A4 (en) * 2017-07-12 2021-04-21 Biomedican, Inc. Production of cannabinoids in yeast
US11981938B2 (en) * 2017-10-05 2024-05-14 Eleszto Genetika, Inc. Microorganisms and methods for the fermentation of cannabinoids

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