SG11201808675VA - Method and kit for the generation of dna libraries for massively parallel sequencing - Google Patents

Method and kit for the generation of dna libraries for massively parallel sequencing

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Publication number
SG11201808675VA
SG11201808675VA SG11201808675VA SG11201808675VA SG11201808675VA SG 11201808675V A SG11201808675V A SG 11201808675VA SG 11201808675V A SG11201808675V A SG 11201808675VA SG 11201808675V A SG11201808675V A SG 11201808675VA SG 11201808675V A SG11201808675V A SG 11201808675VA
Authority
SG
Singapore
Prior art keywords
primer
international
adaptor
sequencing
library
Prior art date
Application number
SG11201808675VA
Inventor
Nicolò Manaresi
Genny Buson
Paola Tononi
Original Assignee
Menarini Silicon Biosystems Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Menarini Silicon Biosystems Spa filed Critical Menarini Silicon Biosystems Spa
Publication of SG11201808675VA publication Critical patent/SG11201808675VA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/143—Magnetism, e.g. magnetic label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 11M111111111101111011111 0101111101110101111111011111H101111111111110111111 International Bureau 0.. .... .. ..... ..or::,„, (10) International Publication Number (43) International Publication Date WO 2017/178655 Al 19 October 2017(19.10.2017) WIPO I PCT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12Q 1/68 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, PCT/EP2017/059075 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, 14 April 2017 (14.04.2017) KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, (25) Filing Language: English NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (26) Publication Language: English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (30) Priority Data: ZA, ZM, ZW. 102016000039154 15 April 2016 (15.04.2016) IT (84) Designated States (unless otherwise indicated, for every (71) Applicant: MENARINI SILICON BIOSYSTEMS kind of regional protection available): ARIPO (BW, GH, S.P.A. [IT/IT]; Via Giuseppe di Vittorio, 21B/3, 40013 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, CASTEL MAGGIORE (IT). TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (72) Inventors: MANARESI, Nicoll); Via Santo Stefano, 132, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, 40125 BOLOGNA (IT). BUSON, Genny; c/o MENARINI DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SILICON BIOSYSTEMS S.p.A., Via Giuseppe di Vit- SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, torio, 21B/3, 40013 CASTEL MAGGIORE (IT). GW, KM, ML, MR, NE, SN, TD, TG). _ TONONI, Paola; c/o MENARINI SILICON BIOSYS- — TEMS S.p.A., Via Giuseppe di Vittorio, 21B/3, 40013 Published: — CASTEL MAGGIORE (IT). — — with international search report (Art. 21(3)) = (74) Agents: BOSIA, Alessandra et al.; STUDIO TORTA 10121 TORINO S.p.A., Via Viotti, 9, (IT). = = = (54) Title: METHOD AND KIT FOR THE GENERATION OF DNA LIBRARIES FOR MASSIVELY PARALLEL SEQUEN- CING = _ SSONN _ NSB ~6 = 566 = = VMS = ' 1 - R -2PRSBC ' PPR33 .,..- , ' - ' ' , SS / - , , Figure 3 1PR / - BC k / Il , 11 in 8SLINA 11 in ,tD GC Ir---- (57) : There is disclosed a method of generating a massively parallel sequencing library comprising the steps of :a) provid- ing a primary WGA DNA library (pWGAlib), including fragments comprising a WGA library universal sequence adaptor;b)re-amp - 11------ lifying the primary WGA DNA library using at least one first primer (1PR) and at least one second primer (2PR); the at least one 1-1 C first primer (1PR) comprising from 5' to 3' at least one first sequencing adaptor (1PR5SA), at least one first sequencing barcode N (1PR5BC) and a first primer 3' section (1PR3S) hybridizing to either the WGA library universal sequence adaptor or its reverse 0 complementary; the at least one second primer (2PR) comprising from 5' to 3' at least one second sequencing adaptor (2PR5SA) dif - ferent from the at least one first sequencing adaptor (1PR5SA), and a second primer 3' section (2PR3S) hybridizing to either the WGA library universal sequence adaptor or its reverse complementary.
SG11201808675VA 2016-04-15 2017-04-14 Method and kit for the generation of dna libraries for massively parallel sequencing SG11201808675VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITUA2016A002640A ITUA20162640A1 (en) 2016-04-15 2016-04-15 METHOD AND KIT FOR THE GENERATION OF DNA LIBRARIES FOR PARALLEL MAXIMUM SEQUENCING
PCT/EP2017/059075 WO2017178655A1 (en) 2016-04-15 2017-04-14 Method and kit for the generation of dna libraries for massively parallel sequencing

Publications (1)

Publication Number Publication Date
SG11201808675VA true SG11201808675VA (en) 2018-11-29

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SG10202010198VA SG10202010198VA (en) 2016-04-15 2017-04-14 Method and kit for the generation of dna libraries for massively parallel sequencing
SG11201808675VA SG11201808675VA (en) 2016-04-15 2017-04-14 Method and kit for the generation of dna libraries for massively parallel sequencing

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SG10202010198VA SG10202010198VA (en) 2016-04-15 2017-04-14 Method and kit for the generation of dna libraries for massively parallel sequencing

Country Status (9)

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US (2) US20200299680A1 (en)
EP (1) EP3443115A1 (en)
JP (1) JP6899844B2 (en)
KR (1) KR102354422B1 (en)
CN (1) CN109477245A (en)
CA (1) CA3019714A1 (en)
IT (1) ITUA20162640A1 (en)
SG (2) SG10202010198VA (en)
WO (1) WO2017178655A1 (en)

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EP3431611A1 (en) * 2017-07-21 2019-01-23 Menarini Silicon Biosystems S.p.A. Improved method and kit for the generation of dna libraries for massively parallel sequencing
CN108866155B (en) * 2018-06-11 2022-07-26 中国农业科学院深圳农业基因组研究所 Preparation method of next generation sequencing library
IT201900013335A1 (en) * 2019-07-30 2021-01-30 Menarini Silicon Biosystems Spa METHOD FOR ANALYZING THE LOSS OF ETEROZIGOSIS (LOH) FOLLOWING TOTAL AMPLIFICATION OF THE GENOME BASED ON A DETERMINISTIC RESTRICTION SITE (DRS-WGA)
CN112662748B (en) * 2019-10-15 2023-02-17 骏实生物科技(上海)有限公司 Primer pair combination, method and kit for single cell DNA sequencing library quality identification
IT201900024159A1 (en) 2019-12-16 2021-06-16 Menarini Silicon Biosystems Spa METHOD AND KIT FOR TOTAL AMPLIFICATION OF THE GENOME AND ANALYSIS OF TARGET MOLECULES IN A BIOLOGICAL SAMPLE
CN111005074A (en) * 2019-12-19 2020-04-14 江西海普洛斯医学检验实验室有限公司 DNA library construction kit based on illumina sequencing platform, library construction method and application
CN112592968B (en) * 2020-12-27 2022-07-26 苏州科诺医学检验实验室有限公司 Molecular tag joint for high-throughput sequencing and synthesis method and application thereof
IT202100024101A1 (en) 2021-09-20 2023-03-20 Menarini Silicon Biosystems Spa METHOD FOR ANALYZING THE DEGREE OF SIMILARITY OF AT LEAST TWO SAMPLES USING DETERMINISTIC AMPLIFICATION OF THE WHOLE GENOME BY RESTRICTION SITES (DRS-WGA)
CN117721191B (en) * 2024-02-07 2024-05-10 深圳赛陆医疗科技有限公司 Gene sequencing method, sequencing device, readable storage medium and gene sequencing system

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ITTO20120962A1 (en) 2012-10-31 2014-05-01 Silicon Biosystems Spa METHOD AND KIT TO REVEAL A SEQUENCE OF WILD-TYPE AND / OR MUTATE TARGET DNA
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DK2881739T3 (en) 2013-12-04 2017-08-21 Menarini Silicon Biosystems Spa Method and kit for determining the genome integrity and / or quality of a library of DNA sequences obtained by deterministic restriction site whole-genome amplification
JP2017508471A (en) * 2014-03-28 2017-03-30 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション Accurate detection of rare genetic mutations in next-generation sequencing
WO2015171656A1 (en) * 2014-05-06 2015-11-12 Baylor College Of Medicine Methods of linearly amplifying whole genome of a single cell

Also Published As

Publication number Publication date
CA3019714A1 (en) 2017-10-19
US20200299680A1 (en) 2020-09-24
SG10202010198VA (en) 2020-11-27
US20230250419A1 (en) 2023-08-10
ITUA20162640A1 (en) 2017-10-15
JP2019514360A (en) 2019-06-06
CN109477245A (en) 2019-03-15
EP3443115A1 (en) 2019-02-20
KR102354422B1 (en) 2022-01-21
JP6899844B2 (en) 2021-07-07
KR20190019049A (en) 2019-02-26
WO2017178655A1 (en) 2017-10-19

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