SG11201811048UA - Single-cell transcript sequencing - Google Patents

Single-cell transcript sequencing

Info

Publication number
SG11201811048UA
SG11201811048UA SG11201811048UA SG11201811048UA SG11201811048UA SG 11201811048U A SG11201811048U A SG 11201811048UA SG 11201811048U A SG11201811048U A SG 11201811048UA SG 11201811048U A SG11201811048U A SG 11201811048UA SG 11201811048U A SG11201811048U A SG 11201811048UA
Authority
SG
Singapore
Prior art keywords
international
cells
cell
suite
san francisco
Prior art date
Application number
SG11201811048UA
Inventor
Kenneth J Livak
Richard A Fekete
Original Assignee
Fluidigm Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluidigm Corp filed Critical Fluidigm Corp
Publication of SG11201811048UA publication Critical patent/SG11201811048UA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property MD 1101111 0 DOI HIM 011101 0 0111111 01110011111111111111111111111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\" WO 2018/013723 Al 18 January 2018 (18.01.2018) W I P0 I P C T (51) International Patent Classification: DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, C12Q 1/68 (2006.01) HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (21) International Application Number: KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, PCT/US2017/041770 MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (22) International Filing Date: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 12 July 2017 (12.07.2017) TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (30) Priority Data: UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 62/362,425 14 July 2016 (14.07.2016) US TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, 62/463,487 24 February 2017 (24.02.2017) US EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (71) Applicant: FLUIDIGM CORPORATION [US/US]; TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, 7000 Shoreline Court, Suite 100, South San Francisco, Cal- KM, ML, MR, NE, SN, TD, TG). ifornia 94080 (US). (72) Inventors: LIVAK, Kenneth J.; 7000 Shoreline Court, Declarations under Rule 4.17: Suite 100, South San Francisco, California 94080 (US). — of inventorship (Rule 4.17(iv)) — FEKETE, Richard A.; 7000 Shoreline Court, Suite 100, Published: South San Francisco, California 94080 (US). — with international search report (Art. 21(3)) = (74) Agent: HALIDAY, Emily M. et al.; Weaver Austin Vil- leneuve & Sampson LLP, P.O. Box 70250, Oakland, Cali- = fornia 94612-0250 (US). = Designated States (unless otherwise indicated, for every = (81) kind of national protection available): AE, AG, AL, AM, — AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, = = (54) Title: SINGLE-CELL TRANSCRIPT SEQUENCING = = 1 = = = I I = Fresh = = Fixed — ,-1 .4 ei N IN Figure 10 M ,-1 © (57) : Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a popu- P i) \" lation of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate Il reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a — novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.
SG11201811048UA 2016-07-14 2017-07-12 Single-cell transcript sequencing SG11201811048UA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662362425P 2016-07-14 2016-07-14
US201762463487P 2017-02-24 2017-02-24
PCT/US2017/041770 WO2018013723A1 (en) 2016-07-14 2017-07-12 Single-cell transcript sequencing

Publications (1)

Publication Number Publication Date
SG11201811048UA true SG11201811048UA (en) 2019-01-30

Family

ID=60951893

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201811048UA SG11201811048UA (en) 2016-07-14 2017-07-12 Single-cell transcript sequencing

Country Status (5)

Country Link
EP (1) EP3485043A4 (en)
CN (1) CN109661474A (en)
CA (1) CA3027423A1 (en)
SG (1) SG11201811048UA (en)
WO (1) WO2018013723A1 (en)

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CN103952482A (en) 2009-04-02 2014-07-30 弗卢伊蒂格姆公司 Multi-primer amplification method for barcoding of target nucleic acids
CN103890245B (en) 2011-05-20 2020-11-17 富鲁达公司 Nucleic acid encoding reactions
CN103998394B (en) 2011-08-01 2016-08-17 德诺弗科学公司 Cell capture system and using method
US9404864B2 (en) 2013-03-13 2016-08-02 Denovo Sciences, Inc. System for imaging captured cells
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
US9752181B2 (en) 2013-01-26 2017-09-05 Denovo Sciences, Inc. System and method for capturing and analyzing cells
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
US9856535B2 (en) 2013-05-31 2018-01-02 Denovo Sciences, Inc. System for isolating cells
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
US11117113B2 (en) 2015-12-16 2021-09-14 Fluidigm Corporation High-level multiplex amplification
US10391493B2 (en) 2017-08-29 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
WO2020186094A1 (en) * 2019-03-12 2020-09-17 Universal Sequencing Technology Methods for single cell intracellular capture and its applications
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
US11578322B2 (en) 2019-05-07 2023-02-14 Bio-Rad Laboratories, Inc. System and method for automated single cell processing
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
SE543469C2 (en) * 2019-06-03 2021-03-02 Di Wu Method for creating a cDNA sequencing library
CN114302643B (en) * 2019-06-14 2024-02-27 伯乐实验室有限公司 Systems and methods for automated single cell processing and analysis
CA3151004A1 (en) * 2019-08-14 2021-02-18 iRepertoire, Inc. Probe-capture method for tcr alpha and beta chain vdj-recovery from oligo-dt reverse transcribed rna
US11504719B2 (en) 2020-03-12 2022-11-22 Bio-Rad Laboratories, Inc. System and method for receiving and delivering a fluid for sample processing
WO2021204860A1 (en) * 2020-04-07 2021-10-14 Trivarop Srl Device and method for determining, from a sample previously collected from an individual, that at least one target antibody has been or is present in said individual
US20230212673A1 (en) * 2020-06-09 2023-07-06 Simsen Diagnostics Ab Lymphocyte clonality determination
CN114606301A (en) * 2020-12-07 2022-06-10 中国科学院脑科学与智能技术卓越创新中心 Single cell transcriptome sequencing method

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030170675A1 (en) * 2001-04-11 2003-09-11 The Gov't Of The U.S Of America As Represented By The Secretary Of The Dept. Of Health & Human Serv. Methods of manipulating nucleic acids
CN103952482A (en) * 2009-04-02 2014-07-30 弗卢伊蒂格姆公司 Multi-primer amplification method for barcoding of target nucleic acids
AU2012209329A1 (en) * 2011-01-24 2013-09-12 Epic Sciences, Inc. Methods for obtaining single cells and applications of single cell omics
ES2774165T3 (en) * 2012-06-15 2020-07-17 Univ Texas High-throughput sequencing of multiple transcripts from a single cell
WO2013188831A1 (en) * 2012-06-15 2013-12-19 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
ES2895750T3 (en) * 2014-09-15 2022-02-22 Abvitro Llc High-throughput sequencing of nucleotide libraries
EP3757211A1 (en) * 2014-12-19 2020-12-30 The Broad Institute, Inc. Methods for profiling the t-cell-receptor repertoire

Also Published As

Publication number Publication date
EP3485043A4 (en) 2020-03-25
CN109661474A (en) 2019-04-19
WO2018013723A1 (en) 2018-01-18
WO2018013723A9 (en) 2019-03-28
CA3027423A1 (en) 2018-01-18
EP3485043A1 (en) 2019-05-22

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