SG11201903329WA - Method for preparing electrocompetent yeast cells, and method for using said cells - Google Patents

Method for preparing electrocompetent yeast cells, and method for using said cells

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Publication number
SG11201903329WA
SG11201903329WA SG11201903329WA SG11201903329WA SG11201903329WA SG 11201903329W A SG11201903329W A SG 11201903329WA SG 11201903329W A SG11201903329W A SG 11201903329WA SG 11201903329W A SG11201903329W A SG 11201903329WA SG 11201903329W A SG11201903329W A SG 11201903329WA
Authority
SG
Singapore
Prior art keywords
international
cells
yeast
pct
boehmert
Prior art date
Application number
SG11201903329WA
Inventor
Sebastian Bunk
Dominik Maurer
Felix Unverdorben
Original Assignee
Immatics Biotechnologies Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immatics Biotechnologies Gmbh filed Critical Immatics Biotechnologies Gmbh
Publication of SG11201903329WA publication Critical patent/SG11201903329WA/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503Immunoglobulin superfamily
    • C07K14/7051T-cell receptor (TcR)-CD3 complex
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905Stable introduction of foreign DNA into chromosome using homologous recombination in yeast

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1#1110111101110101011111 3E1 0 011101111110111 0011101111111011 OEN Organization 0 International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2018/091396 Al 24 May 2018 (24.05.2018) W I PO I PCT (51) International Patent Classification: C12N 15/90 (2006.01) Cl 2N 13/00 (2006.01) (21) International Application Number: PCT/EP2017/079011 (22) International Filing Date: 13 November 2017 (13.11.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10 2016 121 899.5 15 November 2016 (15.11.2016) DE (71) Applicant: IMMATICS BIOTECHNOLOGIES GMBH [DE/DE]; Paul-Ehrlich-StraBe 15, 72076 Tiibingen (DE). (72) Inventors: BUNK, Sebastian; Gertrud-Baeumer-Str. 19/1, 72074 Tiibingen (DE). MAURER, Dominik; Fleinerweg 7, 72116 Moessingen (DE). UNVERDORBEN, Felix; Mohringer Strasse 157, 70199 Stuttgart (DE). Agent: BOEHMERT & BOEHMERT; Krauss, Jan B., Pettenkoferstrasse 22, 80336 Munich (DE). (74) = Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (81) = _ = DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, = = — = OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. = Designated States (unless otherwise indicated, for every (84) kind of regional protection available): ARIPO (BW, GH, = _ _ GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, = UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, = TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, = EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, = MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, — TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). = — Published: ,- 1 — with international search report (Art. 21(3)) 01 M 1-1 01 O_ 0 (54) Title: METHOD FOR PREPARING ELECTROCOMPETENT YEAST CELLS, AND METHOD FOR USING SAID CELLS 1-1 0 (57) : The present invention relates to improved yeast transformation of yeast cells and yeast cell libraries transformed thereby. ei More specifically, the present invention relates to the transformation of yeast by electroporation.
SG11201903329WA 2016-11-15 2017-11-13 Method for preparing electrocompetent yeast cells, and method for using said cells SG11201903329WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102016121899.5A DE102016121899A1 (en) 2016-11-15 2016-11-15 Process for the preparation of electrocompetent yeast cells and methods of using these cells
PCT/EP2017/079011 WO2018091396A1 (en) 2016-11-15 2017-11-13 Method for preparing electrocompetent yeast cells, and method for using said cells

Publications (1)

Publication Number Publication Date
SG11201903329WA true SG11201903329WA (en) 2019-05-30

Family

ID=60484335

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201903329WA SG11201903329WA (en) 2016-11-15 2017-11-13 Method for preparing electrocompetent yeast cells, and method for using said cells

Country Status (23)

Country Link
US (3) US10683495B2 (en)
EP (1) EP3541947B1 (en)
JP (2) JP7133226B2 (en)
KR (1) KR102194520B1 (en)
CN (1) CN109983130B (en)
AR (1) AR110087A1 (en)
AU (1) AU2017360666C1 (en)
BR (1) BR112019008385A2 (en)
CA (1) CA3043953A1 (en)
DE (1) DE102016121899A1 (en)
DK (1) DK3541947T3 (en)
EA (1) EA201990758A1 (en)
ES (1) ES2952039T3 (en)
IL (1) IL266538A (en)
MA (1) MA46835A (en)
MX (1) MX2019005622A (en)
MY (1) MY191776A (en)
NZ (1) NZ752651A (en)
PH (1) PH12019501001A1 (en)
SG (1) SG11201903329WA (en)
TW (1) TWI720268B (en)
UA (1) UA125076C2 (en)
WO (1) WO2018091396A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102017125888A1 (en) 2017-11-06 2019-05-23 Immatics Biotechnologies Gmbh New engineered T cell receptors and their use in immunotherapy
ES2941965T3 (en) 2017-11-06 2023-05-29 Immatics Biotechnologies Gmbh New engineered T cell receptors and immunotherapies based on their use
DE102019108125B4 (en) 2019-03-28 2022-02-03 Immatics US, Inc. CD28 T-CELL CULTURES, COMPOSITIONS AND METHODS OF USE
US20200297768A1 (en) 2019-03-19 2020-09-24 Immatics US, Inc. Cd28 t cell cultures, compositions, and methods of using thereof
DE102019121007A1 (en) 2019-08-02 2021-02-04 Immatics Biotechnologies Gmbh Antigen binding proteins that specifically bind to MAGE-A
US20230132241A1 (en) 2020-01-15 2023-04-27 Immatics Biotechnologies Gmbh Antigen binding proteins specifically binding prame
BR112023022765A2 (en) 2021-05-05 2024-01-02 Immatics Biotechnologies Gmbh ANTIGEN-BINDING PROTEINS THAT SPECIFICALLY BIND PRAME

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0580737B1 (en) 1991-04-10 2004-06-16 The Scripps Research Institute Heterodimeric receptor libraries using phagemids
CA2697282C (en) 2007-08-20 2018-02-13 Nextera As Pvii phage display
SG188846A1 (en) * 2008-03-03 2013-04-30 Abbott Lab Methods for transforming yeast
WO2009114096A1 (en) 2008-03-13 2009-09-17 Cardiac Pacemakers, Inc. Systems and devices for modulating autonomic tone
MX2013000989A (en) 2010-07-28 2013-03-05 Immunocore Ltd T cell receptors.
EP3237908A1 (en) * 2014-12-22 2017-11-01 Universität Zürich Directed evolution of membrane proteins in eukaryotic cells with a cell wall

Also Published As

Publication number Publication date
US20210355478A1 (en) 2021-11-18
EP3541947B1 (en) 2023-06-14
US10683495B2 (en) 2020-06-16
AR110087A1 (en) 2019-02-20
UA125076C2 (en) 2022-01-05
TWI720268B (en) 2021-03-01
AU2017360666C1 (en) 2021-09-02
DE102016121899A1 (en) 2018-05-17
AU2017360666B2 (en) 2021-01-07
US20180135039A1 (en) 2018-05-17
WO2018091396A1 (en) 2018-05-24
JP2022130501A (en) 2022-09-06
US11104894B2 (en) 2021-08-31
TW201819621A (en) 2018-06-01
CN109983130B (en) 2023-10-20
CA3043953A1 (en) 2018-05-24
IL266538A (en) 2019-07-31
PH12019501001A1 (en) 2019-11-11
JP7133226B2 (en) 2022-09-08
AU2017360666A1 (en) 2019-05-02
DK3541947T3 (en) 2023-08-07
BR112019008385A2 (en) 2019-07-09
ES2952039T3 (en) 2023-10-26
MY191776A (en) 2022-07-14
KR102194520B1 (en) 2020-12-23
KR20190065400A (en) 2019-06-11
EP3541947A1 (en) 2019-09-25
EA201990758A1 (en) 2019-10-31
NZ752651A (en) 2022-01-28
JP2019531751A (en) 2019-11-07
CN109983130A (en) 2019-07-05
US20200263162A1 (en) 2020-08-20
MA46835A (en) 2019-09-25
MX2019005622A (en) 2019-08-14

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