SG11201901628XA - Methods and compositions for spinal cord cells - Google Patents

Methods and compositions for spinal cord cells

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Publication number
SG11201901628XA
SG11201901628XA SG11201901628XA SG11201901628XA SG11201901628XA SG 11201901628X A SG11201901628X A SG 11201901628XA SG 11201901628X A SG11201901628X A SG 11201901628XA SG 11201901628X A SG11201901628X A SG 11201901628XA SG 11201901628X A SG11201901628X A SG 11201901628XA
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SG
Singapore
Prior art keywords
california
international
pct
chip
los angeles
Prior art date
Application number
SG11201901628XA
Inventor
Gad Vatine
Sam Sances
Clive Svendsen
Dhruv Sareen
Alexis J Kerl
Original Assignee
Cedars Sinai Medical Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from PCT/US2016/057724 external-priority patent/WO2017070224A1/en
Application filed by Cedars Sinai Medical Center filed Critical Cedars Sinai Medical Center
Publication of SG11201901628XA publication Critical patent/SG11201901628XA/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • C12M23/00—Constructional details, e.g. recesses, hinges
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    • C12M25/02—Membranes; Filters
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
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Abstract

Figure14 Neuroepithelia 'Fgrr Aod Stage iRSC MN precursors (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 08 March 2018 (08.03.2018) WIPO I PCT ill~~~~~~~~ 011101110VIIIOH olo olo Ho mil Immo ow (10) International Publication Number WO 2018/044934 Al (51) International Patent Classification: A61K 35/24 (2015.01) Cl 2M 3/00 (2006.01) A61K 35/30 (2015.01) Cl 2N 5/0797 (2010.01) (21) International Application Number: PCT/US2017/049193 (22) International Filing Date: 29 August 2017 (29.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/380,780 29 August 2016 (29.08.2016) US PCT/US2016/057724 19 October 2016 (19.10.2016) US 15/352,289 15 November 2016 (15.11.2016) US (71) Applicant: CEDARS-SINAI MEDICAL CENTER [US/US]; 8700 Beverly Blvd., Los Angeles, California 90048 (US). (72) Inventors: VATINE, Gad; 508 1/2 South Ogden Drive, Los Angeles, California 90026 (US). SANCES, Sam; 2021 Ocean Avenue, Apt. 223, Santa Monica, California 90405 (US). SVENDSEN, Clive; 15242 Friends Street, Pacifica Palisades, California 90272 (US). SAREEN, Dhruv; 19375 Crystal Ridge Lane, Porter Ranch, California 91326 (US). KERL, Alexis J.; 8700 Beverly Blvd., Los Angeles, Cali- fornia 90048 (US). (74) Agent: CHEN, Stephen, W. et al.; NIXON PEABODY LLP, 300 South Grand Avenue, Suite 4100, Los Angeles, California 90071-3151 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, = (54) Title: METHODS AND COMPOSITIONS FOR SPINAL CORD CELLS Seat Ink reils per well D30 * SLDM IMDWF12 (57) : Described here are systems and methods for deriving both spinal motor neurons and brain microvascular endothelial cells from induced pluripotent stem cells using distinct methods and combining them in a chip format. Neurons cultured alone in chip microvolume displayed increased calcium transient function and chip-specific gene expression. When seeded with endothelial cells, interaction further enhanced neural function, elicited vascular-neural interaction, niche gene expression with enhanced in vivo-like signatures arising from the chip co-cultures. Development of novel media formulations further allow for improved readout of differen- tiation process, by eliminating additives that otherwise confound differentiation processes and resulting phenotypes. [Continued on next page] WO 2018/044934 Al MIDEDIMOMOIDEIREIDIONOMOINOMMEMOIMIE DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))
SG11201901628XA 2016-08-29 2017-08-29 Methods and compositions for spinal cord cells SG11201901628XA (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662380780P 2016-08-29 2016-08-29
PCT/US2016/057724 WO2017070224A1 (en) 2015-10-19 2016-10-19 Microfluidic model of the blood brain barrier
US15/352,289 US20180057788A1 (en) 2016-08-29 2016-11-15 Development of spinal cord on a microfluidic chip
PCT/US2017/049193 WO2018044934A1 (en) 2016-08-29 2017-08-29 Methods and compositions for spinal cord cells

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SG11201901628XA true SG11201901628XA (en) 2019-03-28

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US (2) US20180057788A1 (en)
EP (2) EP3504319B1 (en)
AU (2) AU2017319168B2 (en)
CA (2) CA3035058A1 (en)
GB (1) GB2568446A (en)
SG (2) SG11201901628XA (en)
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US11414648B2 (en) 2017-03-24 2022-08-16 Cedars-Sinai Medical Center Methods and compositions for production of fallopian tube epithelium
US11767513B2 (en) 2017-03-14 2023-09-26 Cedars-Sinai Medical Center Neuromuscular junction
US11913022B2 (en) 2017-01-25 2024-02-27 Cedars-Sinai Medical Center In vitro induction of mammary-like differentiation from human pluripotent stem cells

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WO2011140441A2 (en) 2010-05-06 2011-11-10 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
CN113249297A (en) 2014-05-28 2021-08-13 儿童医院医疗中心 Methods and systems for converting precursor cells to stomach tissue via directed differentiation
US11584916B2 (en) 2014-10-17 2023-02-21 Children's Hospital Medical Center Method of making in vivo human small intestine organoids from pluripotent stem cells
GB2569058B (en) 2016-01-12 2021-04-14 Cedars Sinai Medical Center A method of osteogenic differentiation in microfluidic tissue culture systems
US11473061B2 (en) 2016-02-01 2022-10-18 Cedars-Sinai Medical Center Systems and methods for growth of intestinal cells in microfluidic devices
EP3452578B1 (en) 2016-05-05 2022-08-10 Children's Hospital Medical Center Methods for the in vitro manufacture of gastric fundus tissue and compositions related to same
NZ753873A (en) 2016-12-05 2023-01-27 Children’S Hospital Medical Center Colonic organoids and methods of making and using same
US11981918B2 (en) 2018-04-06 2024-05-14 Cedars-Sinai Medical Center Differentiation technique to generate dopaminergic neurons from induced pluripotent stem cells
WO2019195798A1 (en) * 2018-04-06 2019-10-10 Cedars-Sinai Medical Center Human pluripotent stem cell derived neurodegenerative disease models on a microfluidic chip
CN109097264B (en) * 2018-07-31 2021-10-15 大连理工大学 Microfluidic cell adsorption chip for cell patch clamp
CN110055176B (en) * 2019-04-29 2020-11-24 大连医科大学附属第二医院 Micro-fluidic chip constructed by bionic lung cancer brain metastasis model and model construction method
US20210109080A1 (en) * 2019-10-15 2021-04-15 University Of South Carolina Cell-Based Biosensor for Early Alzheimer's Disease Detection
EP4217114A4 (en) * 2020-09-22 2024-10-23 Cedars Sinai Medical Center Complex microfluidic models for als including nf biomarkers for als research and drug development

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