SG11201901621VA - Development of spinal cord on a microfluidic chip - Google Patents

Development of spinal cord on a microfluidic chip

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Publication number
SG11201901621VA
SG11201901621VA SG11201901621VA SG11201901621VA SG11201901621VA SG 11201901621V A SG11201901621V A SG 11201901621VA SG 11201901621V A SG11201901621V A SG 11201901621VA SG 11201901621V A SG11201901621V A SG 11201901621VA SG 11201901621V A SG11201901621V A SG 11201901621VA
Authority
SG
Singapore
Prior art keywords
international
street
cells
pct
carroll
Prior art date
Application number
SG11201901621VA
Inventor
S Kerns
Norman Wen
Carol Lucchesi
Christopher Hinojosa
Jacob Fraser
Geraldine Hamilton
Gad Vatine
Samuel Sances
Clive Svendsen
Daniel Levner
Dhruv Sareen
Original Assignee
Emulate Inc
Cedars Sinai Medical Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2016/057724 external-priority patent/WO2017070224A1/en
Application filed by Emulate Inc, Cedars Sinai Medical Center filed Critical Emulate Inc
Publication of SG11201901621VA publication Critical patent/SG11201901621VA/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
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    • C12M25/02—Membranes; Filters
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B01L2300/04—Closures and closing means
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    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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    • C12N2535/10—Patterned coating
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    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties

Abstract

PLASMA TREAT CHIPS `AIR PLUG TOP CHANNEL * COAT BOTTOM CHANNEL WITH COL IV:FN, INCUBATE HOUR AT 37 ° C ' REMOVE COL IV:FN * DRY CHIP FOR 15 MINUTES IN VACUUM DESICCATOR * COAT TOP CHANNEL WITH LAMININ, INCUBATE 2 HOURS AT 37 ° C * PLATE iBMECs ON BOTTOM CHANNEL * WASHED APICAL CHAMBER WITH NEURAL MEDIUM * SEED OF NEURAL CELLS * INCUBATE OVER NIGHT AT 37 ° C FIG. 1 W O 20 18/044885 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 08 March 2018 (08.03.2018) WIPO I PCT olimion °nolo III o Ho mil oimIE (10) International Publication Number WO 2018/044885 Al (51) International Patent Classification: C12N 5/071 (2010.01) C12N 5/0797 (2010.01) C12N 5/079 (2010.01) Cl 2N 15/85 (2006.01) C12N 5/0793 (2010.01) (21) International Application Number: PCT/US2017/049115 (22) International Filing Date: 29 August 2017 (29.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/380,780 29 August 2016 (29.08.2016) US PCT/US2016/057724 19 October 2016 (19.10.2016) US 15/352,289 15 November 2016 (15.11.2016) US (71) Applicants: EMULATE, INC. [US/US]; 27 Drydock, Boston, MA 02210 (US). CEDARS-SINAI MEDICAL CENTER [US/US]; 8700 Beverly Blvd, Los Angeles, CA 90048 (US). (72) Inventors: KERNS, Jordan; 7 Heritage Drive, Reading, MA 01867 (US). WEN, Norman; 4975 Washington Street, Unit 118, West Roxbury, MA 02132 (US). LUCCHESI, Carol; 16 Carroll Ct., Westwood, MA 02090 (US). HINO- JOSA, Christopher; 69 Holworthly Street, Cambridge, MA 02138 (US). FRASER, Jacob; 19 Kington Street, Apt. 2, Somerville, MA 02144 (US). HAMILTON, Geraldine; 509 East 2nd Street, Unit 7, Boston, MA 02127 (US). VATINE, Gad; 508 1/2 South Ogden Drive, Los Ange- les, CA 90026 (US). SANCES, Sam; 2021 Ocean Ave, Apt. 223, Santa Monica, CA 90405 (US). SVENDSEN, Clive; 15242 Friends Street, Pacific Palisades, CA 90272 (US). LEVNER, Daniel; 55 Sewall Ave 5C, Brookline, MA 02446 (US). SAREEN, Dhruv; 19375 Crystal Ridge Lane, Porter Ranch, CA 91326 (US). (74) Agent: CARROLL, Peter, G. et al.; Medlen & Carroll, LLP, 1440 Broadway, Suite 1010, Oakland, CA 94612 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (54) Title: DEVELOPMENT OF SPINAL CORD ON A MICROFLUIDIC CHIP (57) : The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barri- er. Culture of such cells in a microfluidic device, whether alone or in combi- nation with other cells, drives maturation and/or differentiation further than existing systems. [Continued on next page] WO 2018/044885 Al MIDEDIMOMMIDIREEMOMMOHEHMODEVOIS SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))
SG11201901621VA 2016-08-29 2017-08-29 Development of spinal cord on a microfluidic chip SG11201901621VA (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201662380780P 2016-08-29 2016-08-29
PCT/US2016/057724 WO2017070224A1 (en) 2015-10-19 2016-10-19 Microfluidic model of the blood brain barrier
US15/352,289 US20180057788A1 (en) 2016-08-29 2016-11-15 Development of spinal cord on a microfluidic chip
PCT/US2017/049115 WO2018044885A1 (en) 2016-08-29 2017-08-29 Development of spinal cord on a microfluidic chip

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SG11201901621VA true SG11201901621VA (en) 2019-03-28

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US (2) US20180057788A1 (en)
EP (2) EP3503901B1 (en)
AU (2) AU2017319168B2 (en)
CA (2) CA3035058A1 (en)
GB (1) GB2568446A (en)
SG (2) SG11201901621VA (en)
WO (2) WO2018044934A1 (en)

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US11767513B2 (en) 2017-03-14 2023-09-26 Cedars-Sinai Medical Center Neuromuscular junction
US11414648B2 (en) 2017-03-24 2022-08-16 Cedars-Sinai Medical Center Methods and compositions for production of fallopian tube epithelium

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