SG10202010198VA - Method and kit for the generation of dna libraries for massively parallel sequencing - Google Patents

Method and kit for the generation of dna libraries for massively parallel sequencing

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Publication number
SG10202010198VA
SG10202010198VA SG10202010198VA SG10202010198VA SG10202010198VA SG 10202010198V A SG10202010198V A SG 10202010198VA SG 10202010198V A SG10202010198V A SG 10202010198VA SG 10202010198V A SG10202010198V A SG 10202010198VA SG 10202010198V A SG10202010198V A SG 10202010198VA
Authority
SG
Singapore
Prior art keywords
kit
generation
massively parallel
dna libraries
parallel sequencing
Prior art date
Application number
SG10202010198VA
Inventor
Nicolò Manaresi
Genny Buson
Paola Tononi
Original Assignee
Menarini Silicon Biosystems Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Menarini Silicon Biosystems Spa filed Critical Menarini Silicon Biosystems Spa
Publication of SG10202010198VA publication Critical patent/SG10202010198VA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/143—Magnetism, e.g. magnetic label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG10202010198VA 2016-04-15 2017-04-14 Method and kit for the generation of dna libraries for massively parallel sequencing SG10202010198VA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITUA2016A002640A ITUA20162640A1 (en) 2016-04-15 2016-04-15 METHOD AND KIT FOR THE GENERATION OF DNA LIBRARIES FOR PARALLEL MAXIMUM SEQUENCING

Publications (1)

Publication Number Publication Date
SG10202010198VA true SG10202010198VA (en) 2020-11-27

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
SG10202010198VA SG10202010198VA (en) 2016-04-15 2017-04-14 Method and kit for the generation of dna libraries for massively parallel sequencing
SG11201808675VA SG11201808675VA (en) 2016-04-15 2017-04-14 Method and kit for the generation of dna libraries for massively parallel sequencing

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201808675VA SG11201808675VA (en) 2016-04-15 2017-04-14 Method and kit for the generation of dna libraries for massively parallel sequencing

Country Status (9)

Country Link
US (2) US20200299680A1 (en)
EP (1) EP3443115A1 (en)
JP (1) JP6899844B2 (en)
KR (1) KR102354422B1 (en)
CN (1) CN109477245A (en)
CA (1) CA3019714A1 (en)
IT (1) ITUA20162640A1 (en)
SG (2) SG10202010198VA (en)
WO (1) WO2017178655A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3431611A1 (en) * 2017-07-21 2019-01-23 Menarini Silicon Biosystems S.p.A. Improved method and kit for the generation of dna libraries for massively parallel sequencing
CN108866155B (en) * 2018-06-11 2022-07-26 中国农业科学院深圳农业基因组研究所 Preparation method of next generation sequencing library
IT201900013335A1 (en) * 2019-07-30 2021-01-30 Menarini Silicon Biosystems Spa METHOD FOR ANALYZING THE LOSS OF ETEROZIGOSIS (LOH) FOLLOWING TOTAL AMPLIFICATION OF THE GENOME BASED ON A DETERMINISTIC RESTRICTION SITE (DRS-WGA)
CN112662748B (en) * 2019-10-15 2023-02-17 骏实生物科技(上海)有限公司 Primer pair combination, method and kit for single cell DNA sequencing library quality identification
IT201900024159A1 (en) 2019-12-16 2021-06-16 Menarini Silicon Biosystems Spa METHOD AND KIT FOR TOTAL AMPLIFICATION OF THE GENOME AND ANALYSIS OF TARGET MOLECULES IN A BIOLOGICAL SAMPLE
CN111005074A (en) * 2019-12-19 2020-04-14 江西海普洛斯医学检验实验室有限公司 DNA library construction kit based on illumina sequencing platform, library construction method and application
CN112592968B (en) * 2020-12-27 2022-07-26 苏州科诺医学检验实验室有限公司 Molecular tag joint for high-throughput sequencing and synthesis method and application thereof
IT202100024101A1 (en) 2021-09-20 2023-03-20 Menarini Silicon Biosystems Spa METHOD FOR ANALYZING THE DEGREE OF SIMILARITY OF AT LEAST TWO SAMPLES USING DETERMINISTIC AMPLIFICATION OF THE WHOLE GENOME BY RESTRICTION SITES (DRS-WGA)
CN117721191B (en) * 2024-02-07 2024-05-10 深圳赛陆医疗科技有限公司 Gene sequencing method, sequencing device, readable storage medium and gene sequencing system

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AU755499B2 (en) * 1998-09-18 2002-12-12 Micromet Ag DNA amplification of a single cell
JP4480715B2 (en) * 2003-01-29 2010-06-16 ４５４ コーポレーション Double-end sequencing
US8206913B1 (en) * 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
US20040209299A1 (en) * 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
JP5452021B2 (en) 2005-12-22 2014-03-26 キージーン ナムローゼ フェンノートシャップ High-throughput AFLP polymorphism detection method
RU2010142289A (en) * 2008-03-17 2012-04-27 Экспрессив Рисерч Б.В. (Nl) DETECTION OF EXPRESSION-related GENES
CN102559856B (en) * 2010-12-22 2014-03-12 深圳华大基因科技服务有限公司 Method for deleting vector segments in sequencing library
CA2883901C (en) * 2012-09-04 2023-04-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
ITTO20120962A1 (en) 2012-10-31 2014-05-01 Silicon Biosystems Spa METHOD AND KIT TO REVEAL A SEQUENCE OF WILD-TYPE AND / OR MUTATE TARGET DNA
CN104903466B (en) * 2012-11-05 2016-11-23 鲁比康基因组学公司 Bar coding nucleic acid
CN105189780A (en) * 2012-12-03 2015-12-23 以琳生物药物有限公司 Compositions and methods of nucleic acid preparation and analyses
CN105359151B (en) * 2013-03-06 2019-04-05 生命科技股份有限公司 System and method for determining copy number variation
EP2881739B1 (en) * 2013-12-04 2017-05-17 Menarini Silicon Biosystems S.p.A. Method and kit for determining the genome integrity and/or the quality of a library of dna sequences obtained by deterministic restriction site whole genome amplification
US20170016056A1 (en) * 2014-03-28 2017-01-19 Ge Healthcare Bio-Sciences Corp. Accurate detection of rare genetic variants in next generation sequencing
CN106661620B (en) 2014-05-06 2021-11-23 贝勒医学院 Single cell whole genome linear amplification method

Also Published As

Publication number Publication date
US20230250419A1 (en) 2023-08-10
JP6899844B2 (en) 2021-07-07
KR102354422B1 (en) 2022-01-21
JP2019514360A (en) 2019-06-06
CN109477245A (en) 2019-03-15
ITUA20162640A1 (en) 2017-10-15
WO2017178655A1 (en) 2017-10-19
SG11201808675VA (en) 2018-11-29
CA3019714A1 (en) 2017-10-19
US20200299680A1 (en) 2020-09-24
EP3443115A1 (en) 2019-02-20
KR20190019049A (en) 2019-02-26

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