HK1218318A1 - Method for construction of sequencing library of variable regions and method for determining nucleic acid sequences of variable regions - Google Patents

Method for construction of sequencing library of variable regions and method for determining nucleic acid sequences of variable regions

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Publication number
HK1218318A1
HK1218318A1 HK16106264.8A HK16106264A HK1218318A1 HK 1218318 A1 HK1218318 A1 HK 1218318A1 HK 16106264 A HK16106264 A HK 16106264A HK 1218318 A1 HK1218318 A1 HK 1218318A1
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HK
Hong Kong
Prior art keywords
variable regions
construction
nucleic acid
acid sequences
sequencing library
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Application number
HK16106264.8A
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Chinese (zh)
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Publication of HK1218318A1 publication Critical patent/HK1218318A1/en

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HK16106264.8A 2014-09-22 2016-06-02 Method for construction of sequencing library of variable regions and method for determining nucleic acid sequences of variable regions HK1218318A1 (en)

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Application Number Priority Date Filing Date Title
CN201410485822.3A CN105506746A (en) 2014-09-22 2014-09-22 Method for constructing variable region sequencing library, and method for determining variable region nucleic acid sequence

Publications (1)

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HK1218318A1 true HK1218318A1 (en) 2017-02-10

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HK16106264.8A HK1218318A1 (en) 2014-09-22 2016-06-02 Method for construction of sequencing library of variable regions and method for determining nucleic acid sequences of variable regions

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CN (1) CN105506746A (en)
HK (1) HK1218318A1 (en)

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CN105063032A (en) * 2015-08-14 2015-11-18 深圳市瀚海基因生物科技有限公司 Multiple PCR primers and method for constructing leukemia minimal residual disease BCR library based on high-flux sequencing
CN107345240A (en) * 2016-05-12 2017-11-14 眭维国 T cell antigen receptor β chain CDR3 processing method
CN107345242A (en) * 2016-05-12 2017-11-14 眭维国 The processing method of TCR β chain complementary determining regions
CN107345241A (en) * 2016-05-12 2017-11-14 眭维国 B cell antigen receptor H chains CDR3 processing method
CN106047857B (en) * 2016-06-01 2020-04-03 苏州金唯智生物科技有限公司 Method for discovering specific functional antibody
CN106192023A (en) * 2016-08-08 2016-12-07 中国科学院北京基因组研究所 A kind of multiple sequencing library construction method based on multidimensional Index
CN106319066B (en) * 2016-09-14 2020-01-17 中国医学科学院北京协和医院 Primer and kit for detecting Systemic Lupus Erythematosus (SLE) B cell receptor library
CN106283201B (en) * 2016-09-20 2019-08-06 中国医学科学院肿瘤医院 The detection of TCR diversity and library construction based on high-flux sequence
CN106834450A (en) * 2017-01-10 2017-06-13 武汉赛云博生物科技有限公司 A kind of method for the detection of T cell Minimal Residual Disease of Leukemia
CN109136346A (en) * 2017-06-16 2019-01-04 深圳华大基因股份有限公司 A kind of method and its application for improving immune group library multiplex PCR and building library quality
CN107267629A (en) * 2017-07-13 2017-10-20 武汉赛云博生物科技有限公司 A kind of method of the B cell minimal residual detection of leukaemia
CN109251980A (en) * 2017-07-14 2019-01-22 中国人民解放军第八医院 Bladder Cancer T cell spectrum model and its construction method and building system
WO2019061198A1 (en) * 2017-09-28 2019-04-04 深圳华大生命科学研究院 Kit for amplifying tcr full-length sequence and use thereof
CN111164210A (en) * 2017-09-28 2020-05-15 深圳华大生命科学研究院 PCR primer for amplifying TCR full-length sequence and application thereof
CN108004304B (en) * 2017-12-20 2021-04-23 北京旌准医疗科技有限公司 Method for detecting clonality of lymphocyte related gene rearrangement
WO2019227331A1 (en) * 2018-05-30 2019-12-05 广州合谐医疗科技有限公司 Method for constructing variable region sequence library, sequencing method, and kit thereof
CN108707653B (en) * 2018-05-30 2021-11-09 广州合谐医疗科技有限公司 Kit for constructing variable region sequence library and sequencing method of variable region sequence
CN109554440A (en) * 2018-12-26 2019-04-02 山东艾克韦生物技术有限公司 Multi-primers group and the method that human T cells immune group library is constructed based on high-flux sequence using the primer sets
CN110246539B (en) * 2019-04-15 2020-04-28 成都益安博生物技术有限公司 Method and device for evaluating immunity level
CN112143777B (en) * 2020-08-18 2022-07-01 北京臻知医学科技有限责任公司 Primer group for constructing CDR3 region high-throughput sequencing library of human TCR beta and application thereof
CN112301099A (en) * 2020-11-30 2021-02-02 南方科技大学 Primer group for amplifying B lymphocyte immune repertoire and application thereof
CN113736849B (en) * 2021-08-11 2024-03-26 深圳泛因医学有限公司 Method for simultaneously detecting TCR and HLA genotyping and application thereof
CN114107287A (en) * 2021-12-13 2022-03-01 云测智能科技有限公司 Preparation method for comprehensively amplifying humann TCR beta chain library by adopting a small amount of degenerate primers
CN117126921B (en) * 2023-10-26 2024-01-26 立凌生物制药(苏州)有限公司 Library construction method for detecting T cell and B cell immune repertoire, primer and kit thereof

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EP2062982A1 (en) * 2007-11-26 2009-05-27 ImmunID Method for studying the V(D)J combinatorial diversity.
EP2333106A1 (en) * 2009-12-09 2011-06-15 ImmunID Use of the combinatory diversity of the catalogue of T lymphocytes as a cancer prognostic marker
CN102443624B (en) * 2010-10-11 2014-12-10 遵义医学院 Method for simultaneously sequencing multi-sample CDR3 (complementary determining region 3) receptor library with high flux
CN103215255B (en) * 2012-01-19 2015-06-17 深圳华大基因科技有限公司 Primer set for amplifying immunoglobulin light chain CDR3 sequence and application thereof
CN103710454B (en) * 2013-12-31 2016-02-17 南方科技大学 The method of TCR or BCR high-flux sequence and utilize sequence label to correct the method for multiple PCR primer deviation

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