HK1218318A1 - Method for construction of sequencing library of variable regions and method for determining nucleic acid sequences of variable regions - Google Patents
Method for construction of sequencing library of variable regions and method for determining nucleic acid sequences of variable regionsInfo
- Publication number
- HK1218318A1 HK1218318A1 HK16106264.8A HK16106264A HK1218318A1 HK 1218318 A1 HK1218318 A1 HK 1218318A1 HK 16106264 A HK16106264 A HK 16106264A HK 1218318 A1 HK1218318 A1 HK 1218318A1
- Authority
- HK
- Hong Kong
- Prior art keywords
- variable regions
- construction
- nucleic acid
- acid sequences
- sequencing library
- Prior art date
Links
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410485822.3A CN105506746A (en) | 2014-09-22 | 2014-09-22 | Method for constructing variable region sequencing library, and method for determining variable region nucleic acid sequence |
Publications (1)
Publication Number | Publication Date |
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HK1218318A1 true HK1218318A1 (en) | 2017-02-10 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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HK16106264.8A HK1218318A1 (en) | 2014-09-22 | 2016-06-02 | Method for construction of sequencing library of variable regions and method for determining nucleic acid sequences of variable regions |
Country Status (2)
Country | Link |
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CN (1) | CN105506746A (en) |
HK (1) | HK1218318A1 (en) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105063032A (en) * | 2015-08-14 | 2015-11-18 | 深圳市瀚海基因生物科技有限公司 | Multiple PCR primers and method for constructing leukemia minimal residual disease BCR library based on high-flux sequencing |
CN107345240A (en) * | 2016-05-12 | 2017-11-14 | 眭维国 | T cell antigen receptor β chain CDR3 processing method |
CN107345242A (en) * | 2016-05-12 | 2017-11-14 | 眭维国 | The processing method of TCR β chain complementary determining regions |
CN107345241A (en) * | 2016-05-12 | 2017-11-14 | 眭维国 | B cell antigen receptor H chains CDR3 processing method |
CN106047857B (en) * | 2016-06-01 | 2020-04-03 | 苏州金唯智生物科技有限公司 | Method for discovering specific functional antibody |
CN106192023A (en) * | 2016-08-08 | 2016-12-07 | 中国科学院北京基因组研究所 | A kind of multiple sequencing library construction method based on multidimensional Index |
CN106319066B (en) * | 2016-09-14 | 2020-01-17 | 中国医学科学院北京协和医院 | Primer and kit for detecting Systemic Lupus Erythematosus (SLE) B cell receptor library |
CN106283201B (en) * | 2016-09-20 | 2019-08-06 | 中国医学科学院肿瘤医院 | The detection of TCR diversity and library construction based on high-flux sequence |
CN106834450A (en) * | 2017-01-10 | 2017-06-13 | 武汉赛云博生物科技有限公司 | A kind of method for the detection of T cell Minimal Residual Disease of Leukemia |
CN109136346A (en) * | 2017-06-16 | 2019-01-04 | 深圳华大基因股份有限公司 | A kind of method and its application for improving immune group library multiplex PCR and building library quality |
CN107267629A (en) * | 2017-07-13 | 2017-10-20 | 武汉赛云博生物科技有限公司 | A kind of method of the B cell minimal residual detection of leukaemia |
CN109251980A (en) * | 2017-07-14 | 2019-01-22 | 中国人民解放军第八医院 | Bladder Cancer T cell spectrum model and its construction method and building system |
WO2019061198A1 (en) * | 2017-09-28 | 2019-04-04 | 深圳华大生命科学研究院 | Kit for amplifying tcr full-length sequence and use thereof |
CN111164210A (en) * | 2017-09-28 | 2020-05-15 | 深圳华大生命科学研究院 | PCR primer for amplifying TCR full-length sequence and application thereof |
CN108004304B (en) * | 2017-12-20 | 2021-04-23 | 北京旌准医疗科技有限公司 | Method for detecting clonality of lymphocyte related gene rearrangement |
WO2019227331A1 (en) * | 2018-05-30 | 2019-12-05 | 广州合谐医疗科技有限公司 | Method for constructing variable region sequence library, sequencing method, and kit thereof |
CN108707653B (en) * | 2018-05-30 | 2021-11-09 | 广州合谐医疗科技有限公司 | Kit for constructing variable region sequence library and sequencing method of variable region sequence |
CN109554440A (en) * | 2018-12-26 | 2019-04-02 | 山东艾克韦生物技术有限公司 | Multi-primers group and the method that human T cells immune group library is constructed based on high-flux sequence using the primer sets |
CN110246539B (en) * | 2019-04-15 | 2020-04-28 | 成都益安博生物技术有限公司 | Method and device for evaluating immunity level |
CN112143777B (en) * | 2020-08-18 | 2022-07-01 | 北京臻知医学科技有限责任公司 | Primer group for constructing CDR3 region high-throughput sequencing library of human TCR beta and application thereof |
CN112301099A (en) * | 2020-11-30 | 2021-02-02 | 南方科技大学 | Primer group for amplifying B lymphocyte immune repertoire and application thereof |
CN113736849B (en) * | 2021-08-11 | 2024-03-26 | 深圳泛因医学有限公司 | Method for simultaneously detecting TCR and HLA genotyping and application thereof |
CN114107287A (en) * | 2021-12-13 | 2022-03-01 | 云测智能科技有限公司 | Preparation method for comprehensively amplifying humann TCR beta chain library by adopting a small amount of degenerate primers |
CN117126921B (en) * | 2023-10-26 | 2024-01-26 | 立凌生物制药(苏州)有限公司 | Library construction method for detecting T cell and B cell immune repertoire, primer and kit thereof |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2062982A1 (en) * | 2007-11-26 | 2009-05-27 | ImmunID | Method for studying the V(D)J combinatorial diversity. |
EP2333106A1 (en) * | 2009-12-09 | 2011-06-15 | ImmunID | Use of the combinatory diversity of the catalogue of T lymphocytes as a cancer prognostic marker |
CN102443624B (en) * | 2010-10-11 | 2014-12-10 | 遵义医学院 | Method for simultaneously sequencing multi-sample CDR3 (complementary determining region 3) receptor library with high flux |
CN103215255B (en) * | 2012-01-19 | 2015-06-17 | 深圳华大基因科技有限公司 | Primer set for amplifying immunoglobulin light chain CDR3 sequence and application thereof |
CN103710454B (en) * | 2013-12-31 | 2016-02-17 | 南方科技大学 | The method of TCR or BCR high-flux sequence and utilize sequence label to correct the method for multiple PCR primer deviation |
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2014
- 2014-09-22 CN CN201410485822.3A patent/CN105506746A/en active Pending
-
2016
- 2016-06-02 HK HK16106264.8A patent/HK1218318A1/en unknown
Also Published As
Publication number | Publication date |
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CN105506746A (en) | 2016-04-20 |
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