AR104024A1 - Métodos y composiciones para la introgresión acelerada de rasgos - Google Patents
Métodos y composiciones para la introgresión acelerada de rasgosInfo
- Publication number
- AR104024A1 AR104024A1 ARP160100742A ARP160100742A AR104024A1 AR 104024 A1 AR104024 A1 AR 104024A1 AR P160100742 A ARP160100742 A AR P160100742A AR P160100742 A ARP160100742 A AR P160100742A AR 104024 A1 AR104024 A1 AR 104024A1
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- AR
- Argentina
- Prior art keywords
- gene
- plant
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- methods
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
- C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Nutrition Science (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
Abstract
Se proporcionan composiciones y métodos para usar genes inhibidores del polen y/o genes marcadores de color en la introgresión acelerada de rasgos. Se proporcionan, además, composiciones y métodos para introducir un gen inhibidor de polen y/o un gen marcador de color cercano a un locus de rasgo de interés; Además, se describen métodos de mejoramiento genético así como métodos para seleccionar plantas que comprenden un locus de rasgo de interés cercano al menos a un gen inhibidor de polen y/o a un gen marcador de color. Los métodos y composiciones usan al menos un gen inhibidor de polen y/o un gen marcador de color para proporcionar un sistema efectivo para realizar la introgresión de rasgo acelerada en el genoma de una planta. Reivindicación 1: Un método para introducir un gen inhibidor de polen cercano a un locus de rasgo de interés en el genoma de una planta progenie; el método comprende: (a) proporcionar una primera planta que tiene una ventana genómica de al menos un gen de rasgo de interés integrado a un primer sitio objetivo ubicado próximo a un telómero, en donde la ventana genómica es de aproximadamente 10 cM de longitud, en donde la primera planta no comprende un gen inhibidor de polen; (b) mejorar genéticamente una primera planta con una segunda planta, en donde la segunda planta comprende en la ventana genómica un gen inhibidor de polen integrado en un segundo sitio objetivo ubicado próximo al telómero y al gen de rasgo de interés de (a); y (c) seleccionar una planta progenie de la etapa (b) que comprende el gen de rasgo de interés y el gen inhibidor de polen, en donde el gen de rasgo de interés y el gen inhibidor de polen están genéticamente unidos.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562135261P | 2015-03-19 | 2015-03-19 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR104024A1 true AR104024A1 (es) | 2017-06-21 |
Family
ID=55646891
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP160100742A AR104024A1 (es) | 2015-03-19 | 2016-03-18 | Métodos y composiciones para la introgresión acelerada de rasgos |
Country Status (7)
Country | Link |
---|---|
US (2) | US11492632B2 (es) |
EP (2) | EP4118955A1 (es) |
CN (1) | CN107429258A (es) |
AR (1) | AR104024A1 (es) |
BR (1) | BR112017017279A2 (es) |
CA (1) | CA2975279A1 (es) |
WO (1) | WO2016149352A1 (es) |
Families Citing this family (13)
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MX2016002306A (es) | 2013-08-22 | 2016-07-08 | Du Pont | Promotor u6 de polimerasa iii de soja y metodos de uso. |
WO2018102131A1 (en) * | 2016-11-29 | 2018-06-07 | Pioneer Hi-Bred International, Inc. | Transcriptional terminators for gene expression in plants |
EP3547825A4 (en) * | 2016-12-02 | 2020-07-01 | Syngenta Participations AG | SIMULTANEOUS EDITING AND HAPLOID INDUCTION |
WO2019165168A1 (en) | 2018-02-23 | 2019-08-29 | Pioneer Hi-Bred International, Inc. | Novel cas9 orthologs |
CN111868247A (zh) | 2018-03-12 | 2020-10-30 | 先锋国际良种公司 | 用于植物转化的方法 |
CN108220333B (zh) * | 2018-03-28 | 2021-01-05 | 中国农业科学院作物科学研究所 | 一种高效植物受体孤雌生殖单倍体筛选方法 |
CN113166744A (zh) | 2018-12-14 | 2021-07-23 | 先锋国际良种公司 | 用于基因组编辑的新颖crispr-cas系统 |
US11807862B2 (en) | 2019-01-15 | 2023-11-07 | Seminis Vegetable Seeds, Inc. | Green bean plants with improved disease resistance |
MX2021008494A (es) | 2019-01-15 | 2021-08-19 | Seminis Vegetable Seeds Inc | Plantas de frijol verde con mejorada resistencia a enfermedades. |
CA3123457A1 (en) * | 2019-03-11 | 2020-09-17 | Pioneer Hi-Bred International, Inc. | Methods for clonal plant production |
WO2022165507A1 (en) * | 2021-01-28 | 2022-08-04 | Central Coast Agriculture, Inc. | Marker-assisted breeding in cannabis plants |
US20230266293A1 (en) * | 2021-09-21 | 2023-08-24 | Pairwise Plants Services, Inc. | Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same |
WO2023247773A1 (en) * | 2022-06-24 | 2023-12-28 | Meiogenix | Induction of meiotic recombination using a crispr system |
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2016
- 2016-03-16 BR BR112017017279A patent/BR112017017279A2/pt not_active Application Discontinuation
- 2016-03-16 EP EP22186775.7A patent/EP4118955A1/en active Pending
- 2016-03-16 US US15/554,234 patent/US11492632B2/en active Active
- 2016-03-16 WO PCT/US2016/022621 patent/WO2016149352A1/en active Application Filing
- 2016-03-16 CN CN201680016751.XA patent/CN107429258A/zh active Pending
- 2016-03-16 CA CA2975279A patent/CA2975279A1/en not_active Abandoned
- 2016-03-16 EP EP16713689.4A patent/EP3270685B1/en active Active
- 2016-03-18 AR ARP160100742A patent/AR104024A1/es unknown
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2022
- 2022-09-12 US US17/931,142 patent/US20230020758A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
EP4118955A1 (en) | 2023-01-18 |
EP3270685B1 (en) | 2023-07-19 |
CN107429258A (zh) | 2017-12-01 |
US11492632B2 (en) | 2022-11-08 |
US20230020758A1 (en) | 2023-01-19 |
BR112017017279A2 (pt) | 2018-04-17 |
EP3270685A1 (en) | 2018-01-24 |
CA2975279A1 (en) | 2016-09-22 |
US20180119162A1 (en) | 2018-05-03 |
WO2016149352A1 (en) | 2016-09-22 |
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