AR112465A1 - Métodos y composiciones para la edición de genes de base viral en plantas - Google Patents

Métodos y composiciones para la edición de genes de base viral en plantas

Info

Publication number
AR112465A1
AR112465A1 ARP180102140A AR112465A1 AR 112465 A1 AR112465 A1 AR 112465A1 AR P180102140 A ARP180102140 A AR P180102140A AR 112465 A1 AR112465 A1 AR 112465A1
Authority
AR
Argentina
Prior art keywords
methods
plant
target site
editing
compositions
Prior art date
Application number
Other languages
English (en)
Original Assignee
Reynolds Tobacco Co R
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reynolds Tobacco Co R filed Critical Reynolds Tobacco Co R
Publication of AR112465A1 publication Critical patent/AR112465A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B13/00—Tobacco for pipes, for cigars, e.g. cigar inserts, or for cigarettes; Chewing tobacco; Snuff
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/20—Biochemical treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/00021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/00041—Use of virus, viral particle or viral elements as a vector
    • C12N2770/00043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente divulgación proporciona composiciones y métodos para editar un sitio diana de un genoma de planta mediante el suministro de componentes de edición funcionales utilizando virus mosaico del tabaco modificado (mTMV). Los métodos divulgados en la presente pueden utilizarse para suministrar un sistema de edición de genes, tal como una ADN endonucleasa, a una célula de planta de tabaco para la modificación de un sitio diana del genoma de la planta. Además, los métodos y composiciones divulgados en la presente proporcionan la producción de una molécula que codifica una meganucleasa in vitro antes del suministro del ARN a una célula de planta. Después de la introducción de la molécula de ácido nucleico que codifica un componente de edición funcional y subsiguiente expresión de los componentes de edición funcionales, la planta puede cultivarse y permitírsele producir semillas que tienen una edición en el sitio diana genómico. Las semillas pueden seguidamente experimentar un rescate de embriones y se los puede cultivar para producir una planta modificada sin material genético heterólogo.
ARP180102140 2017-07-31 2018-07-30 Métodos y composiciones para la edición de genes de base viral en plantas AR112465A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201762539160P 2017-07-31 2017-07-31

Publications (1)

Publication Number Publication Date
AR112465A1 true AR112465A1 (es) 2019-10-30

Family

ID=63165583

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180102140 AR112465A1 (es) 2017-07-31 2018-07-30 Métodos y composiciones para la edición de genes de base viral en plantas

Country Status (6)

Country Link
US (1) US20190093117A1 (es)
EP (1) EP3661354A1 (es)
JP (2) JP2020529206A (es)
AR (1) AR112465A1 (es)
CA (1) CA3071440C (es)
WO (1) WO2019027861A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115052979A (zh) * 2019-11-12 2022-09-13 马里兰大学派克分院 植物载体、组合物及其相关用途
CN111154798B (zh) * 2020-02-18 2021-07-20 杭州师范大学 马铃薯x病毒在诱导番茄种子胎萌中的应用及应用方法
CN114763556B (zh) * 2020-12-31 2024-04-26 北京市农林科学院 一种基因编辑效率提高的引导碱基编辑系统及其应用
CN113774081B (zh) * 2021-05-11 2023-12-05 南京师范大学 一种基因编辑载体及其编辑基因的方法和应用

Family Cites Families (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2927188A (en) 1958-10-22 1960-03-01 George W Green Air conditioning unit
US4528993A (en) 1982-08-20 1985-07-16 R. J. Reynolds Tobacco Company Process for producing moist snuff
US4660577A (en) 1982-08-20 1987-04-28 R.J. Reynolds Tobacco Company Dry pre-mix for moist snuff
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
EP0388186A1 (en) 1989-03-17 1990-09-19 E.I. Du Pont De Nemours And Company External regulation of gene expression
US5231020A (en) 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5034323A (en) 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5310667A (en) 1989-07-17 1994-05-10 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
ES2187497T3 (es) 1990-04-12 2003-06-16 Syngenta Participations Ag Promotores preferentemente en tejidos.
ES2171391T3 (es) 1990-04-26 2002-09-16 Aventis Cropscience Nv Nueva cepa de bacillus thuringiensis y su gen de codificado de toxina insecticida.
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5866775A (en) 1990-09-28 1999-02-02 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5668295A (en) 1990-11-14 1997-09-16 Philip Morris Incorporated Protein involved in nicotine synthesis, DNA encoding, and use of sense and antisense DNAs corresponding thereto to affect nicotine content in transgenic tobacco cells and plants
US5277905A (en) 1991-01-16 1994-01-11 Mycogen Corporation Coleopteran-active bacillus thuringiensis isolate
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
DE69227911T2 (de) 1991-08-02 1999-05-12 Kubota Corp., Tokio/Tokyo Neuer mikroorganismus und insektizid
DE69230290T2 (de) 1991-08-27 2000-07-20 Novartis Ag, Basel Proteine mit insektiziden eigenschaften gegen homopteran insekten und ihre verwendung im pflanzenschutz
EP0612208B1 (en) 1991-10-04 2004-09-15 North Carolina State University Pathogen-resistant transgenic plants
US5327149A (en) 1992-05-18 1994-07-05 Hughes Missile Systems Company R.F. transparent RF/UV-IR detector apparatus
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5387416A (en) 1993-07-23 1995-02-07 R. J. Reynolds Tobacco Company Tobacco composition
US5470353A (en) 1993-10-20 1995-11-28 Hollister Incorporated Post-operative thermal blanket
US5593881A (en) 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5539093A (en) 1994-06-16 1996-07-23 Fitzmaurice; Wayne P. DNA sequences encoding enzymes useful in carotenoid biosynthesis
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5792931A (en) 1994-08-12 1998-08-11 Pioneer Hi-Bred International, Inc. Fumonisin detoxification compositions and methods
US5736514A (en) 1994-10-14 1998-04-07 Nissan Chemical Industries, Ltd. Bacillus strain and harmful organism controlling agents
US5637785A (en) 1994-12-21 1997-06-10 The Salk Institute For Biological Studies Genetically modified plants having modulated flower development
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US5705624A (en) 1995-12-27 1998-01-06 Fitzmaurice; Wayne Paul DNA sequences encoding enzymes useful in phytoene biosynthesis
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
CA2318662A1 (en) 1998-01-16 1999-07-22 Biosource Technologies, Inc. Method of determining the function of nucleotide sequences and the proteins they encode by transfecting the same into a host
ES2273127T3 (es) 1998-02-26 2007-05-01 Pioneer Hi-Bred International, Inc. Promotor alfa-tubulin 3-18 del maiz.
ATE309362T1 (de) 1998-08-20 2005-11-15 Pioneer Hi Bred Int Samen-bevorzugende promotoren
AU5788299A (en) 1998-08-28 2000-03-21 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from (end) genes
WO2000066748A1 (en) 1999-04-29 2000-11-09 Syngenta Limited Herbicide resistant plants
EP1313868B1 (en) 2000-08-30 2006-07-19 North Carolina State University Transgenic plants containing molecular decoys that alter protein content therein
AU2001287862B2 (en) 2000-09-29 2006-12-14 Syngenta Limited Herbicide resistant plants
ES2380178T3 (es) 2001-03-08 2012-05-09 Michigan State University Reguladores del metabolismo de lípidos en plantas
US7208659B2 (en) 2001-05-02 2007-04-24 Conopco Inc. Process for increasing the flavonoid content of a plant and plants obtainable thereby
US6730832B1 (en) 2001-09-10 2004-05-04 Luis Mayan Dominguez High threonine producing lines of Nicotiana tobacum and methods for producing
US7045684B1 (en) 2002-08-19 2006-05-16 Mertec, Llc Glyphosate-resistant plants
US7025066B2 (en) 2002-10-31 2006-04-11 Jerry Wayne Lawson Method of reducing the sucrose ester concentration of a tobacco mixture
WO2006022784A1 (en) 2004-08-23 2006-03-02 U.S. Smokeless Tobacco Company Nicotiana compositions
US10066233B2 (en) 2005-08-26 2018-09-04 Dupont Nutrition Biosciences Aps Method of modulating cell resistance
US8021867B2 (en) 2005-10-18 2011-09-20 Duke University Rationally-designed meganucleases with altered sequence specificity and DNA-binding affinity
CN101686731B (zh) 2007-02-23 2013-05-08 美国无烟烟草有限责任公司 新型烟草组合物及制备方法
US8186360B2 (en) 2007-04-04 2012-05-29 R.J. Reynolds Tobacco Company Cigarette comprising dark air-cured tobacco
AU2008318430A1 (en) 2007-10-31 2009-05-07 Precision Biosciences, Inc. Rationally-designed single-chain meganucleases with non-palindromic recognition sequences
WO2009114321A2 (en) 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Rationally-designed meganucleases for maize genome engineering
EA201391373A1 (ru) 2011-03-23 2014-07-30 Пайонир Хай-Бред Интернэшнл, Инк. Способы получения сложного локуса трансгенных признаков
US9254001B2 (en) 2011-04-27 2016-02-09 R.J. Reynolds Tobacco Company Tobacco-derived components and materials
US10563215B2 (en) 2012-12-21 2020-02-18 Philip Morris Products S.A. Tobacco specific nitrosamine reduction in plants
US9155334B2 (en) 2013-04-05 2015-10-13 R.J. Reynolds Tobacco Company Modification of bacterial profile of tobacco
US11584936B2 (en) * 2014-06-12 2023-02-21 King Abdullah University Of Science And Technology Targeted viral-mediated plant genome editing using CRISPR /Cas9
KR20170081268A (ko) * 2014-11-27 2017-07-11 단지거 이노베이션즈 엘티디. 게놈 편집용 핵산 구조체
US20190359993A1 (en) * 2017-02-15 2019-11-28 National Agriculture And Food Research Organization Method for producing genome-edited plant utilizing plant virus vectors

Also Published As

Publication number Publication date
WO2019027861A1 (en) 2019-02-07
JP2020529206A (ja) 2020-10-08
US20190093117A1 (en) 2019-03-28
CA3071440C (en) 2023-09-26
CA3071440A1 (en) 2019-02-07
JP2024028733A (ja) 2024-03-05
EP3661354A1 (en) 2020-06-10

Similar Documents

Publication Publication Date Title
AR112465A1 (es) Métodos y composiciones para la edición de genes de base viral en plantas
BR112018076027A2 (pt) método para modificar uma sequência-alvo no genoma de uma célula vegetal; método para editar uma sequência de nucleotídeos no genoma de uma célula vegetal; método para modificar simultaneamente múltiplas sequências-alvo no genoma de uma célula vegetal; método para modificar uma sequênciaalvo de dna no genoma de uma célula vegetal e modelo de modificação de polinucleotídeo
AR111790A1 (es) Método para la producción de una planta resistente a herbicidas mediante la introducción de un sistema de edición de bases de un gen relacionado con la resistencia a herbicidas
BR112018008134A2 (pt) método para restaurar a função de um produto gênico não funcional no genoma de uma célula, método para editar uma sequência nucleotídica no genoma de uma célula, planta ou planta de progênie, método para editar uma sequência nucleotídica no genoma de uma célula sem o uso de um molde polinucleotídico de modificação e método para entregar um complexo de rna guia/endonuclease cas numa célula
BR112017000621A2 (pt) método para melhorar um traço agronômico de uma planta, método para melhorar a produtividade de grãos ou a eficiência de uso de nitrogênio de uma planta de milho ou de cultura, método para editar, substituir ou eliminar uma sequência reguladora, método para inserir um promotor ou elemento do promotor no genoma de uma célula vegetal e método para fornecer um perfil de expressão adicional para um polinucleotídeo endógeno de uma célula vegetal
AR075356A1 (es) Promotores inducibles por patogenos y su uso en el mejoramiento de la resistencia a las enfermedades en plantas
BR112019002455A2 (pt) método para edição de base em plantas
AR100216A1 (es) Edición de genes multiplexados
CO2017003427A2 (es) Moleculas de acido nucleico de la subunidad gamma coatomero copi que confieren resistencia a plagas de coleopteros y hemipteros.
AR104024A1 (es) Métodos y composiciones para la introgresión acelerada de rasgos
AR098299A1 (es) Locus óptimos del maíz
BR112018007061A2 (pt) método e composição para aumentar a eficiência de produção de uma planta editada pelo genoma a partir de um protoplasto vegetal e planta regenerada a partir do mesmo
CO2017003543A2 (es) Moleculas de acido nucleico de la subunidad beta coatomero copi que confieren resistencia a plagas de coleopteros y hemipteros
EA201490056A1 (ru) Способы и композиции для получения растений, обладающих мужской стерильностью
AR101080A1 (es) Transformación de maíz haploide con una nucleasa específica de sitio
ECSP15001671A (es) Plantas que tienen una mayor tolerancia a herbicidas
AR085533A1 (es) Metodos de produccion de un locus de rasgos transgenicos complejo
AR118316A2 (es) Elementos regulatorios de plantas y sus usos
BR112017013406B1 (pt) Polinucleotídeo indutor haploide, métodos: para gerar uma planta haploide, para gerar uma planta duplo-haploide, para facilitar uma troca de citoplasma, para gerar uma planta, vetor, seu uso, uso de uma sequência de nucleotídeos, e uso de uma planta, célula de planta, parte de planta ou semente
PH12020551719A1 (en) Genes, constructs and maize event dp-202216-6
MX368807B (es) Metodos para reproducir plantas asexualmente y composiciones de las mismas.
AR086365A1 (es) Elementos reguladores de las plantas y sus usos
AR107260A1 (es) Una soja transgénica resistente al glifosato y el método de preparación de la misma
UY37414A (es) Optimización dirigida de genomas en plantas
CL2024000004A1 (es) Monocotiledóneas heterocigotas con cenh3, su uso para inducción de haploides y edición simultánea del genoma