BR112018007061A2 - método e composição para aumentar a eficiência de produção de uma planta editada pelo genoma a partir de um protoplasto vegetal e planta regenerada a partir do mesmo - Google Patents

método e composição para aumentar a eficiência de produção de uma planta editada pelo genoma a partir de um protoplasto vegetal e planta regenerada a partir do mesmo

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Publication number
BR112018007061A2
BR112018007061A2 BR112018007061A BR112018007061A BR112018007061A2 BR 112018007061 A2 BR112018007061 A2 BR 112018007061A2 BR 112018007061 A BR112018007061 A BR 112018007061A BR 112018007061 A BR112018007061 A BR 112018007061A BR 112018007061 A2 BR112018007061 A2 BR 112018007061A2
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Brazil
Prior art keywords
plant
increasing
production efficiency
edited
genome
Prior art date
Application number
BR112018007061A
Other languages
English (en)
Inventor
Kim Hyeran
Wook WOO Je
Soo Kim Jin
Kim Jungeun
Il Kwon Soon
Choe Sunghwa
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Aict
Inst Basic Science
Seoul Nat Univ R&Db Foundation
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Application filed by Aict, Inst Basic Science, Seoul Nat Univ R&Db Foundation filed Critical Aict
Publication of BR112018007061A2 publication Critical patent/BR112018007061A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C12N15/8298—Brassinosteroids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/14—Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce
    • A01H6/1472—Lactuca sativa [lettuce]
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

método e composição para aumentar a eficiência de produção de uma planta editada pelo genoma a partir de um protoplasto vegetal e planta regenerada a partir do mesmo, através da utilização de uma proteínas cas-ribonucleoproteína de rna guia (rnp). de acordo com a presente invenção, o método de aumentar a eficiência de produção de plantas editadas por genes torna possível produzir eficientemente plantas mutadas em genes alvo e minimizar a introdução de dna estranho em plantas. assim, a presente invenção pode ser muito vantajosamente utilizada em uma ampla variedade de campos, incluindo agricultura, alimentos e biotecnologia.
BR112018007061A 2015-10-06 2016-10-06 método e composição para aumentar a eficiência de produção de uma planta editada pelo genoma a partir de um protoplasto vegetal e planta regenerada a partir do mesmo BR112018007061A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20150140314 2015-10-06
PCT/KR2016/011216 WO2017061805A1 (ko) 2015-10-06 2016-10-06 식물 원형질체로부터 유전체 교정 식물체를 고효율로 제조하는 방법

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BR112018007061A2 true BR112018007061A2 (pt) 2019-01-15

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BR112018007070A BR112018007070A2 (pt) 2015-10-06 2016-10-06 método, composição e kit para a preparação de uma planta a partir de um protoplasto, planta regenerada e composição para clivagem de dna

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US (3) US11149281B2 (pt)
EP (2) EP3359674A4 (pt)
JP (3) JP6916188B2 (pt)
KR (2) KR102107735B1 (pt)
CN (3) CN111961683A (pt)
AU (3) AU2016336565B2 (pt)
BR (2) BR112018007061A2 (pt)
CA (3) CA3001108A1 (pt)
MX (2) MX2018004264A (pt)
WO (2) WO2017061805A1 (pt)

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KR102264215B1 (ko) * 2020-12-23 2021-06-11 전남대학교산학협력단 유전자 교정을 이용한 아스코르브산 함량이 증가된 토마토 식물체의 제조방법 및 상기 제조방법에 의해 제조된 토마토 식물체
KR102280955B1 (ko) * 2021-06-07 2021-07-23 전남대학교산학협력단 토마토 열매의 아스코르브산 함량을 조절하는 토마토 유래 apx4 유전자 및 이의 용도
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BR112018007061A2 (pt) 2015-10-06 2019-01-15 Aict método e composição para aumentar a eficiência de produção de uma planta editada pelo genoma a partir de um protoplasto vegetal e planta regenerada a partir do mesmo

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JP2018530348A (ja) 2018-10-18
AU2016336566B2 (en) 2020-05-14
AU2019204375B2 (en) 2021-05-27
BR112018007070A2 (pt) 2019-06-04
US20180312869A1 (en) 2018-11-01
US11149281B2 (en) 2021-10-19
US20210340554A1 (en) 2021-11-04
US20190338298A1 (en) 2019-11-07
EP3366777A4 (en) 2019-03-20
KR20170041640A (ko) 2017-04-17
CA3001108A1 (en) 2017-04-13
CN108473998A (zh) 2018-08-31
JP2018530350A (ja) 2018-10-18
US11136588B2 (en) 2021-10-05
KR20170041641A (ko) 2017-04-17
JP6855543B2 (ja) 2021-04-07
AU2016336566A1 (en) 2018-05-24
KR101829885B1 (ko) 2018-03-30
JP6885954B2 (ja) 2021-06-16
CN111961683A (zh) 2020-11-20
JP2020022455A (ja) 2020-02-13
CN108368516A (zh) 2018-08-03
JP6916188B2 (ja) 2021-08-11
CA3001232C (en) 2020-07-07
MX2018004265A (es) 2018-11-09
CA3001232A1 (en) 2017-04-13
EP3359674A1 (en) 2018-08-15
WO2017061806A1 (en) 2017-04-13
WO2017061805A1 (ko) 2017-04-13
AU2016336565B2 (en) 2019-07-18
CN108473998B (zh) 2020-11-20
MX2018004264A (es) 2018-11-09
EP3366777A1 (en) 2018-08-29
AU2019204375A1 (en) 2019-07-11
AU2016336565A1 (en) 2018-05-17
CA3080849A1 (en) 2017-04-13
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EP3359674A4 (en) 2019-03-13

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