AR122346A2 - Métodos y composiciones para la regulación selectiva de la expresión de proteínas - Google Patents

Métodos y composiciones para la regulación selectiva de la expresión de proteínas

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Publication number
AR122346A2
AR122346A2 ARP200103370A ARP200103370A AR122346A2 AR 122346 A2 AR122346 A2 AR 122346A2 AR P200103370 A ARP200103370 A AR P200103370A AR P200103370 A ARP200103370 A AR P200103370A AR 122346 A2 AR122346 A2 AR 122346A2
Authority
AR
Argentina
Prior art keywords
compositions
methods
protein expression
mts
seq
Prior art date
Application number
ARP200103370A
Other languages
English (en)
Inventor
Jintai Huang
Youlin Qi
Heping Yang
Yuanji Zhang
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR122346A2 publication Critical patent/AR122346A2/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12Processes for modifying agronomic input traits, e.g. crop yield
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A01H3/04Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823Reproductive tissue-specific promoters
    • C12N15/8231Male-specific, e.g. anther, tapetum, pollen
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275Glyphosate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289Male sterility
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/10923-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y205/00Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/010193-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

Abstract

La invención proporciona moléculas de ADN recombinante novedosas, composiciones y métodos para regular selectivamente la expresión de una molécula de polinucleótido que se puede transcribir o proteína recombinante en un tejido reproductor masculino de una planta transgénica. La invención también proporciona plantas, células de plantas, partes de plantas, semillas y productos básicos transgénicos que comprenden dichas composiciones y moléculas de ADN. Reivindicación 1: Una molécula de ADN recombinante, caracterizada porque comprende un elemento diana de mts-ARNip que comprende SEQ ID Nº 1, o SEQ ID Nº 2, o complementos de longitud completa de estas en donde el elemento diana de mts-ARNip está unido operativamente a una molécula de polinucleótido heterólogo.
ARP200103370A 2015-07-22 2020-12-03 Métodos y composiciones para la regulación selectiva de la expresión de proteínas AR122346A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562195546P 2015-07-22 2015-07-22

Publications (1)

Publication Number Publication Date
AR122346A2 true AR122346A2 (es) 2022-09-07

Family

ID=57835207

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP160102157A AR105374A1 (es) 2015-07-22 2016-07-15 Métodos y composiciones para la regulación selectiva de la expresión de proteínas
ARP200103370A AR122346A2 (es) 2015-07-22 2020-12-03 Métodos y composiciones para la regulación selectiva de la expresión de proteínas

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP160102157A AR105374A1 (es) 2015-07-22 2016-07-15 Métodos y composiciones para la regulación selectiva de la expresión de proteínas

Country Status (26)

Country Link
US (3) US10704057B2 (es)
EP (1) EP3324732B1 (es)
JP (1) JP6930960B2 (es)
KR (1) KR20180026777A (es)
CN (2) CN114891758B (es)
AR (2) AR105374A1 (es)
AU (3) AU2016296468B2 (es)
BR (1) BR112018001192B1 (es)
CA (1) CA2992113A1 (es)
CL (1) CL2018000165A1 (es)
CO (1) CO2018000499A2 (es)
EA (1) EA037898B1 (es)
EC (1) ECSP18003799A (es)
ES (1) ES2938362T3 (es)
HR (1) HRP20230022T1 (es)
HU (1) HUE061144T2 (es)
MA (1) MA42497A (es)
MX (2) MX2018000890A (es)
PE (1) PE20180465A1 (es)
PH (1) PH12018500119A1 (es)
PL (1) PL3324732T3 (es)
PT (1) PT3324732T (es)
RS (1) RS63980B1 (es)
UA (1) UA126893C2 (es)
WO (1) WO2017015043A1 (es)
ZA (1) ZA201800175B (es)

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EP2726493B1 (en) 2011-07-01 2020-10-14 Monsanto Technology LLC Methods and compositions for selective regulation of protein expression
BR112018001192B1 (pt) 2015-07-22 2024-02-06 Monsanto Technology Llc Molécula de dna recombinante, método para regular seletivamente a expressão de uma proteína em um tecido reprodutivo masculino de uma planta transgênica, e uso de uma planta ou semente que compreende a referida molécula de dna recombinante
BR112020014164A2 (pt) 2018-02-02 2020-12-08 Monsanto Technology Llc Evento de milho mon87429 e métodos de uso do mesmo
MY195997A (en) * 2018-04-18 2023-02-27 Kimagri Corp Sdn Bhd Recombinant Gene

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Publication number Publication date
ZA201800175B (en) 2018-12-19
CO2018000499A2 (es) 2018-05-31
US20240102042A1 (en) 2024-03-28
PL3324732T3 (pl) 2023-03-13
EP3324732A1 (en) 2018-05-30
AR105374A1 (es) 2017-09-27
AU2016296468B2 (en) 2020-08-27
CN107846856A (zh) 2018-03-27
US10704057B2 (en) 2020-07-07
WO2017015043A1 (en) 2017-01-26
CN114891758B (zh) 2024-02-13
US20170029841A1 (en) 2017-02-02
MA42497A (fr) 2018-05-30
EP3324732A4 (en) 2019-05-15
MX2021000869A (es) 2021-03-29
UA126893C2 (uk) 2023-02-22
CA2992113A1 (en) 2017-01-26
US20200283790A1 (en) 2020-09-10
AU2020273330B2 (en) 2023-07-20
CN114891758A (zh) 2022-08-12
HRP20230022T1 (hr) 2023-03-03
CN107846856B (zh) 2022-05-24
HUE061144T2 (hu) 2023-05-28
ECSP18003799A (es) 2018-03-31
RS63980B1 (sr) 2023-03-31
BR112018001192A2 (pt) 2018-09-25
AU2023229538A1 (en) 2023-10-05
US11866721B2 (en) 2024-01-09
KR20180026777A (ko) 2018-03-13
AU2016296468A1 (en) 2018-02-08
MX2018000890A (es) 2018-05-15
PT3324732T (pt) 2023-02-14
ES2938362T3 (es) 2023-04-10
EP3324732B1 (en) 2022-11-23
CL2018000165A1 (es) 2018-04-27
EA201890328A1 (ru) 2018-06-29
AU2020273330A1 (en) 2020-12-17
PH12018500119A1 (en) 2018-07-23
PE20180465A1 (es) 2018-03-06
BR112018001192B1 (pt) 2024-02-06
JP6930960B2 (ja) 2021-09-01
JP2018521658A (ja) 2018-08-09
EA037898B1 (ru) 2021-06-03

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