CO2018007568A2 - Composiciones y métodos para el direccionamiento eficaz de transgenes - Google Patents

Composiciones y métodos para el direccionamiento eficaz de transgenes

Info

Publication number
CO2018007568A2
CO2018007568A2 CONC2018/0007568A CO2018007568A CO2018007568A2 CO 2018007568 A2 CO2018007568 A2 CO 2018007568A2 CO 2018007568 A CO2018007568 A CO 2018007568A CO 2018007568 A2 CO2018007568 A2 CO 2018007568A2
Authority
CO
Colombia
Prior art keywords
methods
plants
addressing
compositions
efficient transgene
Prior art date
Application number
CONC2018/0007568A
Other languages
English (en)
Inventor
Aihua Shao
Christine M Ellis
Michael E Goley
Clayton T Larue
Sherry L Leclere
Qungang Qi
Kwan Y Thai
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of CO2018007568A2 publication Critical patent/CO2018007568A2/es

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/001Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221Transit peptides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04Plant cells or tissues
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0069Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y103/00Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/03Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
    • C12Y103/03004Protoporphyrinogen oxidase (1.3.3.4)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/08Fusion polypeptide containing a localisation/targetting motif containing a chloroplast localisation signal

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La invención provee moléculas de ADN recombinante y construcciones útiles para proveer localización subcelular de transgén eficaz de proteínas en plantas transgénicas. También se provén moléculas de ADN recombinante y construcciones para conferir tolerancia o resistencia a herbicidas a plantas, así como plantas que presentan tolerancia a herbicidas y métodos para producir o utilizar dichas plantas.
CONC2018/0007568A 2015-12-21 2018-07-19 Composiciones y métodos para el direccionamiento eficaz de transgenes CO2018007568A2 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562270180P 2015-12-21 2015-12-21
US201662364715P 2016-07-20 2016-07-20
PCT/US2016/067531 WO2017112589A1 (en) 2015-12-21 2016-12-19 Compositions and methods for efficient targeting of transgenes

Publications (1)

Publication Number Publication Date
CO2018007568A2 true CO2018007568A2 (es) 2018-07-31

Family

ID=59065936

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2018/0007568A CO2018007568A2 (es) 2015-12-21 2018-07-19 Composiciones y métodos para el direccionamiento eficaz de transgenes

Country Status (25)

Country Link
US (1) US10563220B2 (es)
EP (2) EP4219729A3 (es)
JP (2) JP6961611B2 (es)
KR (1) KR20180089518A (es)
CN (2) CN108463554B (es)
AU (2) AU2016377487B2 (es)
BR (1) BR102016029899A2 (es)
CA (1) CA2950803A1 (es)
CO (1) CO2018007568A2 (es)
DK (1) DK3394272T5 (es)
EC (1) ECSP18054745A (es)
ES (1) ES2941913T3 (es)
HR (1) HRP20230311T1 (es)
HU (1) HUE061632T2 (es)
MX (1) MX2018007705A (es)
PE (2) PE20181540A1 (es)
PH (1) PH12018501317A1 (es)
PL (1) PL3394272T3 (es)
PT (1) PT3394272T (es)
RS (1) RS64091B1 (es)
RU (1) RU2751238C2 (es)
UA (1) UA126554C2 (es)
UY (1) UY37040A (es)
WO (1) WO2017112589A1 (es)
ZA (1) ZA201803723B (es)

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* Cited by examiner, † Cited by third party
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ES2975746T3 (es) 2015-08-03 2024-07-12 Monsanto Technology Llc Métodos y composiciones para tolerancia a los herbicidas en plantas
US10378023B2 (en) 2015-09-01 2019-08-13 Monsanto Technology Llc Methods and compositions for herbicide tolerance in plants
US10863743B2 (en) * 2016-07-29 2020-12-15 Basf Se Method for controlling PPO resistant weeds
US10745712B2 (en) 2016-07-29 2020-08-18 Monsanto Technology Llc Methods and compositions for gene expression in plants
CA3026528A1 (en) * 2017-12-15 2019-06-15 Monsanto Technology Llc Methods and compositions for ppo herbicide tolerance
BR112020012211A2 (pt) 2017-12-20 2020-11-24 Sumitomo Chemical Company, Limited método para controlar organismos nocivos em safras
CA3057917A1 (en) * 2018-10-16 2020-04-16 Monsanto Technology Llc Brassica event mon94100 and methods of use thereof
WO2023044364A1 (en) 2021-09-15 2023-03-23 Enko Chem, Inc. Protoporphyrinogen oxidase inhibitors
PE20241633A1 (es) 2022-01-14 2024-08-09 Enko Chem Inc Inhibidores de protoporfirinogeno oxidasa
US20240229063A1 (en) * 2022-12-20 2024-07-11 Monsanto Technology Llc Transgenic corn event zm_csm63715 and methods for detection and uses thereof

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Also Published As

Publication number Publication date
ZA201803723B (en) 2019-07-31
US20170175131A1 (en) 2017-06-22
JP2022009102A (ja) 2022-01-14
CA2950803A1 (en) 2017-06-21
DK3394272T3 (da) 2023-04-03
UA126554C2 (uk) 2022-11-02
UY37040A (es) 2017-07-31
EP3394272B1 (en) 2023-01-11
JP2019500060A (ja) 2019-01-10
RU2751238C2 (ru) 2021-07-12
RU2018124597A (ru) 2020-01-23
HUE061632T2 (hu) 2023-07-28
EP3394272A1 (en) 2018-10-31
EP3394272A4 (en) 2019-07-31
AU2016377487B2 (en) 2020-12-24
AU2020281170B2 (en) 2021-05-20
RU2018124597A3 (es) 2020-05-28
CN108463554A (zh) 2018-08-28
US10563220B2 (en) 2020-02-18
RS64091B1 (sr) 2023-04-28
CN116355928A (zh) 2023-06-30
WO2017112589A1 (en) 2017-06-29
AU2020281170A1 (en) 2021-02-04
PH12018501317A1 (en) 2019-02-18
BR102016029899A2 (pt) 2017-06-27
ES2941913T3 (es) 2023-05-26
PT3394272T (pt) 2023-04-11
CN108463554B (zh) 2023-04-18
DK3394272T5 (da) 2024-09-30
EP4219729A2 (en) 2023-08-02
AU2020281170C1 (en) 2021-11-18
HRP20230311T1 (hr) 2023-05-12
JP6961611B2 (ja) 2021-11-05
PE20181540A1 (es) 2018-09-26
PE20240226A1 (es) 2024-02-16
AU2016377487A1 (en) 2018-06-28
PL3394272T3 (pl) 2023-05-08
ECSP18054745A (es) 2018-08-31
KR20180089518A (ko) 2018-08-08
MX2018007705A (es) 2018-11-09
EP4219729A3 (en) 2023-09-27
JP7312805B2 (ja) 2023-07-21

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