PE20240226A1 - Composiciones y metodos para el direccionamiento eficaz de transgenes - Google Patents

Composiciones y metodos para el direccionamiento eficaz de transgenes

Info

Publication number
PE20240226A1
PE20240226A1 PE2023001714A PE2023001714A PE20240226A1 PE 20240226 A1 PE20240226 A1 PE 20240226A1 PE 2023001714 A PE2023001714 A PE 2023001714A PE 2023001714 A PE2023001714 A PE 2023001714A PE 20240226 A1 PE20240226 A1 PE 20240226A1
Authority
PE
Peru
Prior art keywords
seq
dna
sequence encoding
ctp
transgenes
Prior art date
Application number
PE2023001714A
Other languages
English (en)
Inventor
Christine M Ellis
Michael E Goley
Clayton T Larue
Sherry L Leclere
Qungang Qi
Aihua Shao
Kwan Y Thai
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of PE20240226A1 publication Critical patent/PE20240226A1/es

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
    • C12Y103/03004—Protoporphyrinogen oxidase (1.3.3.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/08—Fusion polypeptide containing a localisation/targetting motif containing a chloroplast localisation signal

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La invencion se refiere a una molecula de ADN recombinante que comprende una secuencia de ADN codificante de un peptido de transito de cloroplastos (CTP) unida operativamente a una secuencia de ADN codificante de una dicamba monooxigenasa (DMO) o una protoporfirinogeno oxidasa (PPO), donde el CTP comprende una secuencia de SEQ ID NO: 3; en donde la secuencia de ADN codificante del CTP comprende una secuencia que se selecciona del grupo que consiste en SEQ ID NO:13-14 y la DMO o PPO comprende una secuencia polipeptidica seleccionada del grupo que consiste en SEQ ID NO: 18-27 y 40-59. Tambien se refiere a una construccion de ADN que comprende la citada molecula de ADN unida operativamente a un promotor heterologo funcional en una celula vegetal y un metodo para producir una planta con tolerancia a herbicidas.
PE2023001714A 2015-12-21 2016-12-19 Composiciones y metodos para el direccionamiento eficaz de transgenes PE20240226A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562270180P 2015-12-21 2015-12-21
US201662364715P 2016-07-20 2016-07-20
PCT/US2016/067531 WO2017112589A1 (en) 2015-12-21 2016-12-19 Compositions and methods for efficient targeting of transgenes

Publications (1)

Publication Number Publication Date
PE20240226A1 true PE20240226A1 (es) 2024-02-16

Family

ID=59065936

Family Applications (2)

Application Number Title Priority Date Filing Date
PE2018001186A PE20181540A1 (es) 2015-12-21 2016-12-19 Composiciones y metodos para el direccionamiento eficaz de transgenes
PE2023001714A PE20240226A1 (es) 2015-12-21 2016-12-19 Composiciones y metodos para el direccionamiento eficaz de transgenes

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PE2018001186A PE20181540A1 (es) 2015-12-21 2016-12-19 Composiciones y metodos para el direccionamiento eficaz de transgenes

Country Status (25)

Country Link
US (1) US10563220B2 (es)
EP (2) EP4219729A3 (es)
JP (2) JP6961611B2 (es)
KR (1) KR20180089518A (es)
CN (2) CN108463554B (es)
AU (2) AU2016377487B2 (es)
BR (1) BR102016029899A2 (es)
CA (1) CA2950803A1 (es)
CO (1) CO2018007568A2 (es)
DK (1) DK3394272T3 (es)
EC (1) ECSP18054745A (es)
ES (1) ES2941913T3 (es)
HR (1) HRP20230311T1 (es)
HU (1) HUE061632T2 (es)
MX (1) MX2018007705A (es)
PE (2) PE20181540A1 (es)
PH (1) PH12018501317A1 (es)
PL (1) PL3394272T3 (es)
PT (1) PT3394272T (es)
RS (1) RS64091B1 (es)
RU (1) RU2751238C2 (es)
UA (1) UA126554C2 (es)
UY (1) UY37040A (es)
WO (1) WO2017112589A1 (es)
ZA (1) ZA201803723B (es)

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* Cited by examiner, † Cited by third party
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HRP20240421T1 (hr) 2015-08-03 2024-06-21 Monsanto Technology Llc Postupci i pripravci za otpornost biljaka na herbicide
US10378023B2 (en) 2015-09-01 2019-08-13 Monsanto Technology Llc Methods and compositions for herbicide tolerance in plants
PE20190838A1 (es) 2016-07-29 2019-06-17 Monsanto Technology Llc Metodos y composiciones para la expresion genica en plantas
BR112019001645B1 (pt) * 2016-07-29 2022-10-04 Basf Se Método para controlar o crescimento de ervas daninhas resistentes a ppo, uso dos compostos e uso da composição
CA3026528A1 (en) * 2017-12-15 2019-06-15 Monsanto Technology Llc Methods and compositions for ppo herbicide tolerance
CA3086453A1 (en) * 2017-12-20 2018-03-08 Sumitomo Chemical Company, Limited Method for controlling harmful organisms in crops
CA3057917A1 (en) * 2018-10-16 2020-04-16 Monsanto Technology Llc Brassica event mon94100 and methods of use thereof
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WO2023137309A2 (en) 2022-01-14 2023-07-20 Enko Chem, Inc. Protoporphyrinogen oxidase inhibitors

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CA3026528A1 (en) 2017-12-15 2019-06-15 Monsanto Technology Llc Methods and compositions for ppo herbicide tolerance

Also Published As

Publication number Publication date
UA126554C2 (uk) 2022-11-02
ZA201803723B (en) 2019-07-31
US10563220B2 (en) 2020-02-18
JP6961611B2 (ja) 2021-11-05
RU2018124597A (ru) 2020-01-23
JP2019500060A (ja) 2019-01-10
CO2018007568A2 (es) 2018-07-31
PH12018501317A1 (en) 2019-02-18
CN116355928A (zh) 2023-06-30
RU2751238C2 (ru) 2021-07-12
PE20181540A1 (es) 2018-09-26
PL3394272T3 (pl) 2023-05-08
CN108463554B (zh) 2023-04-18
CN108463554A (zh) 2018-08-28
RU2018124597A3 (es) 2020-05-28
EP3394272A1 (en) 2018-10-31
UY37040A (es) 2017-07-31
EP3394272B1 (en) 2023-01-11
HRP20230311T1 (hr) 2023-05-12
PT3394272T (pt) 2023-04-11
EP4219729A2 (en) 2023-08-02
EP4219729A3 (en) 2023-09-27
US20170175131A1 (en) 2017-06-22
AU2016377487A1 (en) 2018-06-28
EP3394272A4 (en) 2019-07-31
KR20180089518A (ko) 2018-08-08
JP2022009102A (ja) 2022-01-14
CA2950803A1 (en) 2017-06-21
AU2016377487B2 (en) 2020-12-24
ES2941913T3 (es) 2023-05-26
MX2018007705A (es) 2018-11-09
JP7312805B2 (ja) 2023-07-21
AU2020281170B2 (en) 2021-05-20
BR102016029899A2 (pt) 2017-06-27
WO2017112589A1 (en) 2017-06-29
DK3394272T3 (da) 2023-04-03
RS64091B1 (sr) 2023-04-28
HUE061632T2 (hu) 2023-07-28
AU2020281170A1 (en) 2021-02-04
AU2020281170C1 (en) 2021-11-18
ECSP18054745A (es) 2018-08-31

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