AR088113A1 - ARN DE INTERFERENCIA (ARNi) PARA EL CONTROL DE HONGOS Y OOMICETOS POR LA INHIBICION DEL GEN DE SACAROPINA DESHIDROGENASA - Google Patents

ARN DE INTERFERENCIA (ARNi) PARA EL CONTROL DE HONGOS Y OOMICETOS POR LA INHIBICION DEL GEN DE SACAROPINA DESHIDROGENASA

Info

Publication number
AR088113A1
AR088113A1 ARP120103685A ARP120103685A AR088113A1 AR 088113 A1 AR088113 A1 AR 088113A1 AR P120103685 A ARP120103685 A AR P120103685A AR P120103685 A ARP120103685 A AR P120103685A AR 088113 A1 AR088113 A1 AR 088113A1
Authority
AR
Argentina
Prior art keywords
sequence
polynucleotide
seq
polypeptide
depicted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
ARP120103685A
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English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Intellectual Property GmbH
Original Assignee
Bayer Intellectual Property GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Intellectual Property GmbH filed Critical Bayer Intellectual Property GmbH
Publication of AR088113A1 publication Critical patent/AR088113A1/es
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60—Isolated nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/713—Double-stranded nucleic acids or oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

Abstract

Reivindicación 1: Una molécula de ARNdc que comprende 1) una primera cadena que comprende una secuencia sustancialmente idéntica a por lo menos 18 nucleótidos contiguos del gen de sacaropina deshidrogenasa de un hongo u oomiceto, y 2) una segunda cadena que comprende una secuencia sustancialmente complementaria con la primera cadena. Reivindicación 2: Una molécula de ARNdc según la reivindicación 1, en donde el gen de hongo u oomiceto se selecciona del grupo consistente en: a) un polinucleótido que comprende una secuencia como la que se representa en las SEC ID Nº 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43; b) un polinucleótido que codifica un polipéptido que tiene una secuencia como la que se representa en las SEC ID Nº 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44; c) un polinucleótido que tiene una identidad de secuencia de al menos 70%, preferentemente de al menos 80%, más preferentemente de al menos 90%, todavía más preferentemente de al menos 95%, con un polinucleótido que tiene una secuencia como la que se representa en las SEC ID Nº: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43; d) un polinucleótido que codifica un polipéptido que tiene una identidad de secuencia de al menos 70%, preferentemente de al menos 80%, más preferentemente de al menos 90%, todavía más preferentemente de al menos 95%, con un polipéptido que tiene una secuencia como la que se representa en las SEC ID Nº 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44; e) un polinucleótido que hibrida bajo condiciones rigurosas con un polinucleótido que tiene una secuencia como la que se representa en las SEC ID Nº 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43; y f) un polinucleótido que hibrida bajo condiciones rigurosas con un polinucleótido que codifica un polipéptido que tiene una identidad de secuencia de al menos 70% con un polipéptido que tiene una secuencia como la que se representa en las SEC ID Nº 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44. Reivindicación 3: Una composición que comprende al menos una molécula de ARNdc según la reivindicación 1 ó 2. Reivindicación 7: Una construcción genética que comprende al menos una secuencia de ADN, así como elemento(s) heterólogo(s) de regulación en las posiciones 5 y, opcionalmente, 3, caracterizada por que la o las secuencias de ADN son capaces de formar una molécula de ARNdc, según las reivindicaciones 1 ó 2. Reivindicación 8: Un vector de donación y/o expresión, caracterizado por que contiene al menos una construcción genética según la reivindicación 7. Reivindicación 9: Una célula de planta transgénica capaz de expresar al menos una molécula de ARNdc según las reivindicaciones 1 ó 2. Reivindicación 10: Una planta o semilla transgénica, o parte de las mismas, que comprende una célula de planta transgénica según la reivindicación 9. Reivindicación 13: Un método para controlar un patógeno vegetal, en particular un hongo u oomiceto, que comprende proporcionar a dicho patógeno una molécula de ARNdc según las reivindicaciones 1 ó 2, o una composición según una cualquiera de las reivindicaciones 3 a 5.
ARP120103685A 2011-10-04 2012-10-03 ARN DE INTERFERENCIA (ARNi) PARA EL CONTROL DE HONGOS Y OOMICETOS POR LA INHIBICION DEL GEN DE SACAROPINA DESHIDROGENASA Pending AR088113A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11356013 2011-10-04
US201261661062P 2012-06-18 2012-06-18

Publications (1)

Publication Number Publication Date
AR088113A1 true AR088113A1 (es) 2014-05-07

Family

ID=48043183

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120103685A Pending AR088113A1 (es) 2011-10-04 2012-10-03 ARN DE INTERFERENCIA (ARNi) PARA EL CONTROL DE HONGOS Y OOMICETOS POR LA INHIBICION DEL GEN DE SACAROPINA DESHIDROGENASA

Country Status (15)

Country Link
US (2) US20150082495A1 (es)
EP (1) EP2764101B1 (es)
JP (1) JP6255344B2 (es)
CN (1) CN103842507A (es)
AR (1) AR088113A1 (es)
AU (1) AU2012320554B2 (es)
BR (1) BR112014008059A2 (es)
CA (1) CA2844868A1 (es)
EA (1) EA028662B1 (es)
ES (1) ES2628436T3 (es)
IN (1) IN2014DN03473A (es)
PL (1) PL2764101T3 (es)
UA (1) UA115132C2 (es)
WO (1) WO2013050410A1 (es)
ZA (1) ZA201403196B (es)

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US10913955B2 (en) 2017-10-10 2021-02-09 Dow Agrosciences Llc Aldehyde dehydrogenase (ALDH1) nucleic acid molecules that control pathogens
US20210323950A1 (en) 2018-06-04 2021-10-21 Bayer Aktiengesellschaft Herbicidally active bicyclic benzoylpyrazoles
CN112888306A (zh) * 2018-08-17 2021-06-01 国家科学研究中心 保护植物防御致病性细菌和/或促进共生和共栖细菌的有益作用的基于rna的生物防治方法
CN111647572A (zh) * 2020-06-01 2020-09-11 中国科学院天津工业生物技术研究所 源于里氏木霉的还原酶及其编码基因和用途
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US20190382770A1 (en) 2019-12-19
ZA201403196B (en) 2015-07-29
JP6255344B2 (ja) 2017-12-27
JP2015501139A (ja) 2015-01-15
UA115132C2 (uk) 2017-09-25
WO2013050410A1 (en) 2013-04-11
EP2764101A1 (en) 2014-08-13
EP2764101B1 (en) 2017-03-29
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CA2844868A1 (en) 2013-04-11
ES2628436T3 (es) 2017-08-02
BR112014008059A2 (pt) 2018-04-24
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