HRP20230311T1 - Pripravci i postupci za učinkovito ciljanje transgena - Google Patents

Pripravci i postupci za učinkovito ciljanje transgena Download PDF

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Publication number
HRP20230311T1
HRP20230311T1 HRP20230311TT HRP20230311T HRP20230311T1 HR P20230311 T1 HRP20230311 T1 HR P20230311T1 HR P20230311T T HRP20230311T T HR P20230311TT HR P20230311 T HRP20230311 T HR P20230311T HR P20230311 T1 HRP20230311 T1 HR P20230311T1
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Croatia
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plant
amount
dna
seq
plant cell
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HRP20230311TT
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English (en)
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Christine M. Ellis
Michael E. Goley
Clayton T. Larue
Sherry L. Leclere
Qungang Qi
Aihua Shao
Kwan Y. Thai
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Monsanto Technology Llc
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Publication of HRP20230311T1 publication Critical patent/HRP20230311T1/hr

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/001Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221Transit peptides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04Plant cells or tissues
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0069Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y103/00Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/03Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
    • C12Y103/03004Protoporphyrinogen oxidase (1.3.3.4)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/08Fusion polypeptide containing a localisation/targetting motif containing a chloroplast localisation signal

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (11)

1. Molekula rekombinantne DNK koja sadrži DNK sekvencu koja kodira tranzitni peptid kloroplasta (CTP) iz SEQ ID NO:1, operativno povezan sa sekvencom DNK koja kodira (i) dikamba monooksigenazu (DMO) iz SEQ ID NO: 18 ili 20, ili (ii) protoporfirinogen oksidazu (PPO) iz SEQ ID NO: 43, 40, 50, ili 54.
2. Molekula rekombinantne DNK prema zahtjevu 1, naznačena time što DNK sekvenca koja kodira CTP sadrži sekvencu odabranu iz skupine koju čine SEQ ID NO:7-11.
3. Molekula rekombinantne DNK prema zahtjevu 1 ili 2, naznačena time što sekvenca DNK koja kodira DMO ili PPO sadrži sekvencu odabranu iz skupine koja sadrži SEQ ID NO: 28, 30, 71, 74, 76, 81, 85, 88, 93, 96, i 98.
4. Konstrukt DNK, naznačen time što sadrži molekulu DNK prema bilo kojem od zahtjeva 1 do 3 operativno povezanu s heterolognim promotorom funkcionalnim u biljnoj stanici.
5. Transgena biljka, biljna stanica, dio biljke ili sjeme koje sadrži molekulu DNK prema bilo kojem od zahtjeva 1 do 3, ili konstrukt DNK iz zahtjeva 4.
6. Transgena biljka, biljna stanica, dio biljke ili sjeme prema zahtjevu 5, naznačeni time što je biljka jednosupnica ili dvosupnica.
7. Postupak za proizvodnju biljke otporne na herbicide naznačen time što obuhvaća korake: a) transformiranje biljne stanice s DNK konstruktom prema zahtjevu 4, i b) regeneriranje biljke iz transformirane biljne stanice koja sadrži DNK konstrukt.
8. Postupak prema zahtjevu 7, naznačen time što je regenerirana biljka tolerantna na herbicid odabran iz skupine koju čine dikamba i inhibitor PPO.
9. Postupak ekspresije dikamba monooksigenaze (DMO) ili protoporfirinogen oksidaze (PPO) koji se sastoji od uvođenja konstrukta DNK prema zahtjevu 4 u biljnu stanicu pomoću (a) Agrobacterium transformacije; (b) bombardiranja česticama DNK; ili (c) umetanja konstrukta DNK u biljni genom na unaprijed određeno mjesto postupcima mutageneze usmjerene na mjesto.
10. Postupak za kontrolu rasta korova u okruženju uzgoja usjeva koji obuhvaća korake: a) sadnju biljke ili sjemena prema zahtjevu 5 u okoliš za uzgoj usjeva; i b) primjenu na okoliš za uzgoj usjeva (i) dikamba u količini između 1,12 kg ai/ha do 17,92 kg ai/ha ili (ii) u slučaju herbicida inhibitora PPO S-3100 u količini od 40-160 g ai/ha, fomesafena u količini od 420-1680 g ai/ha , acifluorfena u količini od 420-1680 g ai/ha, laktofena u količini od 220-880 g ai/ha, flumioksazina u količini od 105-420 g ai/ha, sulfentrazona u količini od 420-1680 g ai/ha, ili saflufenacila u količini od 100-400 g ai/ha.
11. Postupak prema zahtjevu 10, naznačen time što je biljka jednosupnica ili dvosupnica ili njezino sjeme.
HRP20230311TT 2015-12-21 2016-12-19 Pripravci i postupci za učinkovito ciljanje transgena HRP20230311T1 (hr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562270180P 2015-12-21 2015-12-21
US201662364715P 2016-07-20 2016-07-20
PCT/US2016/067531 WO2017112589A1 (en) 2015-12-21 2016-12-19 Compositions and methods for efficient targeting of transgenes
EP16879934.4A EP3394272B1 (en) 2015-12-21 2016-12-19 Compositions and methods for efficient targeting of transgenes

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US (1) US10563220B2 (hr)
EP (2) EP4219729A3 (hr)
JP (2) JP6961611B2 (hr)
KR (1) KR20180089518A (hr)
CN (2) CN116355928A (hr)
AU (2) AU2016377487B2 (hr)
BR (1) BR102016029899A2 (hr)
CA (1) CA2950803A1 (hr)
CO (1) CO2018007568A2 (hr)
DK (1) DK3394272T3 (hr)
EC (1) ECSP18054745A (hr)
ES (1) ES2941913T3 (hr)
HR (1) HRP20230311T1 (hr)
HU (1) HUE061632T2 (hr)
MX (1) MX2018007705A (hr)
PE (2) PE20240226A1 (hr)
PH (1) PH12018501317A1 (hr)
PL (1) PL3394272T3 (hr)
PT (1) PT3394272T (hr)
RS (1) RS64091B1 (hr)
RU (1) RU2751238C2 (hr)
UA (1) UA126554C2 (hr)
UY (1) UY37040A (hr)
WO (1) WO2017112589A1 (hr)
ZA (1) ZA201803723B (hr)

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EP3394272B1 (en) 2023-01-11
PH12018501317A1 (en) 2019-02-18
PE20181540A1 (es) 2018-09-26
HUE061632T2 (hu) 2023-07-28
UA126554C2 (uk) 2022-11-02
CN108463554B (zh) 2023-04-18
PL3394272T3 (pl) 2023-05-08
AU2020281170C1 (en) 2021-11-18
JP2019500060A (ja) 2019-01-10
EP3394272A1 (en) 2018-10-31
ZA201803723B (en) 2019-07-31
CA2950803A1 (en) 2017-06-21
WO2017112589A1 (en) 2017-06-29
AU2020281170B2 (en) 2021-05-20
US10563220B2 (en) 2020-02-18
CN116355928A (zh) 2023-06-30
JP2022009102A (ja) 2022-01-14
RS64091B1 (sr) 2023-04-28
RU2018124597A3 (hr) 2020-05-28
KR20180089518A (ko) 2018-08-08
BR102016029899A2 (pt) 2017-06-27
ES2941913T3 (es) 2023-05-26
RU2751238C2 (ru) 2021-07-12
US20170175131A1 (en) 2017-06-22
MX2018007705A (es) 2018-11-09
AU2016377487A1 (en) 2018-06-28
CO2018007568A2 (es) 2018-07-31
EP4219729A2 (en) 2023-08-02
EP4219729A3 (en) 2023-09-27
AU2016377487B2 (en) 2020-12-24
JP6961611B2 (ja) 2021-11-05
JP7312805B2 (ja) 2023-07-21
UY37040A (es) 2017-07-31
RU2018124597A (ru) 2020-01-23
CN108463554A (zh) 2018-08-28
PE20240226A1 (es) 2024-02-16
AU2020281170A1 (en) 2021-02-04
EP3394272A4 (en) 2019-07-31
PT3394272T (pt) 2023-04-11
ECSP18054745A (es) 2018-08-31
DK3394272T3 (da) 2023-04-03

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