HRP20230022T1 - Postupci i pripravci za selektivnu regulaciju ekspresije proteina - Google Patents
Postupci i pripravci za selektivnu regulaciju ekspresije proteina Download PDFInfo
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- HRP20230022T1 HRP20230022T1 HRP20230022TT HRP20230022T HRP20230022T1 HR P20230022 T1 HRP20230022 T1 HR P20230022T1 HR P20230022T T HRP20230022T T HR P20230022TT HR P20230022 T HRP20230022 T HR P20230022T HR P20230022 T1 HRP20230022 T1 HR P20230022T1
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- transgenic plant
- herbicide
- recombinant dna
- glyphosate
- plant
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- 238000000034 method Methods 0.000 title claims 7
- 230000014493 regulation of gene expression Effects 0.000 title claims 2
- 239000000203 mixture Substances 0.000 title 1
- 241000196324 Embryophyta Species 0.000 claims 19
- 230000009261 transgenic effect Effects 0.000 claims 13
- 239000004009 herbicide Substances 0.000 claims 12
- 230000002363 herbicidal effect Effects 0.000 claims 11
- 102000053602 DNA Human genes 0.000 claims 9
- 108020004511 Recombinant DNA Proteins 0.000 claims 9
- 239000003112 inhibitor Substances 0.000 claims 7
- 108091033319 polynucleotide Proteins 0.000 claims 7
- 102000040430 polynucleotide Human genes 0.000 claims 7
- 239000002157 polynucleotide Substances 0.000 claims 7
- 239000005562 Glyphosate Substances 0.000 claims 5
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 claims 5
- 229940097068 glyphosate Drugs 0.000 claims 5
- QYOJSKGCWNAKGW-HCWXCVPCSA-N shikimate-3-phosphate Chemical compound O[C@H]1CC(C(O)=O)=C[C@H](OP(O)(O)=O)[C@@H]1O QYOJSKGCWNAKGW-HCWXCVPCSA-N 0.000 claims 5
- 239000002253 acid Substances 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 206010021929 Infertility male Diseases 0.000 claims 3
- 208000007466 Male Infertility Diseases 0.000 claims 3
- 230000001939 inductive effect Effects 0.000 claims 3
- 230000001850 reproductive effect Effects 0.000 claims 3
- 108010000700 Acetolactate synthase Proteins 0.000 claims 2
- 102000000452 Acetyl-CoA carboxylase Human genes 0.000 claims 2
- 108010016219 Acetyl-CoA carboxylase Proteins 0.000 claims 2
- 108010060806 Photosystem II Protein Complex Proteins 0.000 claims 2
- 108020001991 Protoporphyrinogen Oxidase Proteins 0.000 claims 2
- 102100029028 Protoporphyrinogen oxidase Human genes 0.000 claims 2
- 230000008685 targeting Effects 0.000 claims 2
- 125000004203 4-hydroxyphenyl group Chemical group [H]OC1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims 1
- 229930192334 Auxin Natural products 0.000 claims 1
- 102000016680 Dioxygenases Human genes 0.000 claims 1
- 108010028143 Dioxygenases Proteins 0.000 claims 1
- 241001057636 Dracaena deremensis Species 0.000 claims 1
- 241000209510 Liliopsida Species 0.000 claims 1
- 239000002363 auxin Substances 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000004720 fertilization Effects 0.000 claims 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 1
- 230000010144 wind-pollination Effects 0.000 claims 1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
- A01H3/04—Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
- C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
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- C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
- C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
- C12Y205/01019—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
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- Pregnancy & Childbirth (AREA)
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- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Claims (14)
1. Molekula rekombinantne DNK koja sadrži ciljni element mts-siRNK koji se sastoji od sekvence odabrane iz skupine koja sadrži SEQ ID NOs: 1, 2, i njihovih komplemenata pri čemu je ciljni elmts-siRNK operativno povezan s heterolognom polinukleotidnom molekulom sposobnom za prepisivanje u molekulu RNK.
2. Molekula rekombinantne DNK prema zahtjevu 1, naznačena time što:
heterologna polinukleotidna molekula koja se može transkribirati, kodira protein koji biljkama daje otpornost na herbicide.
3. Molekula rekombinantne DNK prema zahtjevu 2, naznačena time što je navedeni herbicid odabran iz skupine koja se sastoji od inhibitora acetil koenzima A karboksilaze (ACCase), inhibitora acetolaktat sintaze (ALS), inhibitora fotosustava II (PSII), inhibitora protoporfirinogen oksidaze (PPO), inhibitora 4-hidroksifenil dioksigenaze (HPPD), inhibitora 5-enolipiruvil šikimat 3-fosfat sintaze (EPSPS), inhibitora glutamin sintaze (GS) sintetskih auksina.
4. Molekula rekombinantne DNK prema zahtjevu 1, što navedena heterologna polinukleotidna molekula koja se može transkribirati, kodira 5-enolipiruvil šikimat 3-fosfat sintazu (EPSPS) tolerantnu na glifosat.
5. Transgena biljka, njezin dio ili sjeme, naznačena time što u svom genomu sadrži molekulu rekombinantne DNK prema zahtjevu 1 .
6. Biljka prema zahtjevu 5, naznačena time što je navedena biljka jednosupnica.
7. Biljka prema zahtjevu 6, naznačena time što je navedena biljka, biljka kukuruza.
8. Postupak selektivne regulacije ekspresije proteina u muškom reproduktivnom tkivu transgene biljke, naznačen time što obuhvaća ekspresiju u navedenoj transgenoj biljci rekombinantne DNK molekule prema zahtjevu 1 .
9. Postupak prema zahtjevu 8, naznačen time što navedeni protein sadrži 5-enolipiruvil šikimat 3-fosfat sintazu (EPSPS) tolerantnu na glifosat.
10. Postupak izazivanja muške sterilnosti u transgenoj biljci, naznačen time što obuhvaća:
(a) uzgoj transgene biljke koja sadrži molekulu rekombinantne DNK koja sadrži ciljni element mts-siRNK koji se sastoji od sekvence odabrane iz skupine koja se sastoji od SEQ ID NOs: 1, 2, i njihovih komplemenata, pri čemu je ciljni element mts-siRNK operativan povezan s heterolognom polinukleotidnom molekulom koja kodira protein koji daje toleranciju na barem prvi herbicid; i
(b) primjenu učinkovite količine navedenog herbicida na navedenu transgenu biljku, pri čemu se primjena herbicida provodi prije ili istovremeno s razvojem muškog reproduktivnog tkiva navedene transgene biljke, čime se inducira muška sterilnost transgene biljke.
11. Postupak prema zahtjevu 10, naznačen time što:
(a) navedena heterologna polinukleotidna molekula kodira 5-enolipiruvil šikimat 3-fosfat sintazu (EPSPS) tolerantnu na glifosat; ili
(b) navedeni herbicid je glifosat; ili
(c) navedena učinkovita količina herbicida se primjenjuje u razvojnom stadiju odabranom iz skupine koju čine stadiji V4, VS, V6, V7, V8, V9, V10, V11, V12, V13, i V14.
12. Postupak prema zahtjevu 11, naznačen time što je navedena učinkovita količina herbicida oko 0,125 funti kiselinskog ekvivalenta po jutru do oko 8 funti kiselinskog ekvivalenta po jutru glifosata.
13. Hibridno sjeme proizvedeno postupkom koji obuhvaća:
(a) primjenu učinkovite količine herbicida na transgenu biljku koja sadrži molekulu rekombinantne DNK koja sadrži ciljni element mts-siRNK koji se sastoji od sekvence odabrane iz skupine koja sadrži SEQ ID NOs: 1, 2, i njihove komplemente, pri čemu je ciljni element mts-siRNK operativno povezan s heterolognom polinukleotidnom molekulom koja kodira protein koji daje otpornost na barem prvi herbicid, pri čemu se navedena primjena herbicida provodi prije ili istodobno s razvojem muškog reproduktivnog tkiva transgene biljke, čime se inducira muška sterilnost navedene transgene biljke;
(b) oplodnju navedene transgene biljke polenom iz druge biljke; i
(c) omogućavanje formiranja hibridnog sjemena iz navedene transgene biljke
pri čemu hibridno sjeme sadrži navedenu molekulu rekombinantne DNK.
14. Hibridno sjeme prema zahtjevu 13, naznačeno time što:
(a) navedena oplodnja uključuje dopuštanje oprašivanja vjetrom; ili
(b) navedena transkribirana heterologna polinukleotidna molekula kodira 5-enolipiruvil šikimat 3-fosfat sintazu (EPSPS) tolerantnu na glifosat; ili
(c) navedeni herbicid je glifosat, koji se glifosat poželjno primjenjuje istovremeno s razvojem u učinkovitoj količini od oko 0,125 funti kiselinskog ekvivalenta po jutru do oko 8 funti kiselinskog ekvivalenta po jutru.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562195546P | 2015-07-22 | 2015-07-22 | |
| EP16828263.0A EP3324732B1 (en) | 2015-07-22 | 2016-07-14 | Methods and compositions for selective regulation of protein expression |
| PCT/US2016/042217 WO2017015043A1 (en) | 2015-07-22 | 2016-07-14 | Methods and compositions for selective regulation of protein expression |
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| Publication Number | Publication Date |
|---|---|
| HRP20230022T1 true HRP20230022T1 (hr) | 2023-03-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| HRP20230022TT HRP20230022T1 (hr) | 2015-07-22 | 2016-07-14 | Postupci i pripravci za selektivnu regulaciju ekspresije proteina |
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| Country | Link |
|---|---|
| US (3) | US10704057B2 (hr) |
| EP (1) | EP3324732B1 (hr) |
| JP (1) | JP6930960B2 (hr) |
| KR (1) | KR20180026777A (hr) |
| CN (2) | CN107846856B (hr) |
| AR (2) | AR105374A1 (hr) |
| AU (3) | AU2016296468B2 (hr) |
| BR (1) | BR112018001192B1 (hr) |
| CA (1) | CA2992113A1 (hr) |
| CL (1) | CL2018000165A1 (hr) |
| CO (1) | CO2018000499A2 (hr) |
| EA (1) | EA037898B1 (hr) |
| EC (1) | ECSP18003799A (hr) |
| ES (1) | ES2938362T3 (hr) |
| HR (1) | HRP20230022T1 (hr) |
| HU (1) | HUE061144T2 (hr) |
| MA (1) | MA42497A (hr) |
| MX (2) | MX2018000890A (hr) |
| PE (1) | PE20180465A1 (hr) |
| PH (1) | PH12018500119A1 (hr) |
| PL (1) | PL3324732T3 (hr) |
| PT (1) | PT3324732T (hr) |
| RS (1) | RS63980B1 (hr) |
| UA (1) | UA126893C2 (hr) |
| WO (1) | WO2017015043A1 (hr) |
| ZA (1) | ZA201800175B (hr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| PE20141518A1 (es) | 2011-07-01 | 2014-11-17 | Monsanto Technology Llc | Metodos y composiciones para la regulacion selectiva de la expresion de proteinas |
| BR112018001192B1 (pt) | 2015-07-22 | 2024-02-06 | Monsanto Technology Llc | Molécula de dna recombinante, método para regular seletivamente a expressão de uma proteína em um tecido reprodutivo masculino de uma planta transgênica, e uso de uma planta ou semente que compreende a referida molécula de dna recombinante |
| AU2019215434B2 (en) | 2018-02-02 | 2020-11-05 | Monsanto Technology Llc | Maize event MON87429 and methods of use thereof |
| MY195997A (en) * | 2018-04-18 | 2023-02-27 | Kimagri Corp Sdn Bhd | Recombinant Gene |
| WO2024059543A2 (en) * | 2022-09-14 | 2024-03-21 | Monsanto Technology Llc | Methods and compositions for selective regulation of protein expression |
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| US5583210A (en) | 1993-03-18 | 1996-12-10 | Pioneer Hi-Bred International, Inc. | Methods and compositions for controlling plant development |
| US5750868A (en) * | 1994-12-08 | 1998-05-12 | Pioneer Hi-Bred International, Inc. | Reversible nuclear genetic system for male sterility in transgenic plants |
| US6271439B1 (en) | 1998-03-04 | 2001-08-07 | Pioneer Hi-Bred International, Inc. | Methods and compositions for regulating cell death and enhancing disease resistance to plant pathogens |
| EP1062354A2 (en) | 1998-03-09 | 2000-12-27 | Monsanto Company | Glyphosate as a gametocide |
| CN1249133A (zh) * | 1998-12-25 | 2000-04-05 | 北京大学 | 获得雄性不育植物的分子方法 |
| AR044724A1 (es) | 2000-03-27 | 2005-10-05 | Syngenta Participations Ag | Promotores del virus de la rizadura amarilla del cestrum |
| US20020062499A1 (en) | 2000-05-08 | 2002-05-23 | Conner Timothy W. | Methods for translational repression of gene expression in plants |
| US6646186B1 (en) | 2000-07-26 | 2003-11-11 | Stine Seed Farm Inc. | Hybrid soybeans and methods of production |
| GB0108048D0 (en) | 2001-03-30 | 2001-05-23 | Isis Innovation | Specification of meiocyte and tapetal cell layers in plants |
| US7977046B2 (en) * | 2002-05-10 | 2011-07-12 | Monsanto Technology Llc | Method of selecting DNA constructs for herbicide tolerance in plants |
| US7230168B2 (en) | 2001-12-20 | 2007-06-12 | The Curators Of The University Of Missouri | Reversible male sterility in transgenic plants by expression of cytokinin oxidase |
| US6967656B2 (en) | 2002-10-28 | 2005-11-22 | Syngenta Participations Ag | Growing degree unit meter and method |
| AU2003903132A0 (en) | 2003-06-20 | 2003-07-03 | Molecular Plant Breeding Nominees Ltd. | Plant promoter |
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