HRP20180349T1 - Pojačivač virusa šećerne trske u obliku bacila (scbv) i njegova uporaba u funkcionalnoj genomskoj tehnologiji biljaka - Google Patents
Pojačivač virusa šećerne trske u obliku bacila (scbv) i njegova uporaba u funkcionalnoj genomskoj tehnologiji biljaka Download PDFInfo
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- HRP20180349T1 HRP20180349T1 HRP20180349TT HRP20180349T HRP20180349T1 HR P20180349 T1 HRP20180349 T1 HR P20180349T1 HR P20180349T T HRP20180349T T HR P20180349TT HR P20180349 T HRP20180349 T HR P20180349T HR P20180349 T1 HRP20180349 T1 HR P20180349T1
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- plant
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- 241000196324 Embryophyta Species 0.000 title claims 32
- 239000003623 enhancer Substances 0.000 title claims 7
- 240000000111 Saccharum officinarum Species 0.000 title claims 3
- 235000007201 Saccharum officinarum Nutrition 0.000 title claims 3
- 230000003612 virological effect Effects 0.000 title 1
- 230000035897 transcription Effects 0.000 claims 11
- 238000013518 transcription Methods 0.000 claims 11
- 230000001105 regulatory effect Effects 0.000 claims 10
- 230000009261 transgenic effect Effects 0.000 claims 8
- 240000008042 Zea mays Species 0.000 claims 6
- 108091033319 polynucleotide Proteins 0.000 claims 4
- 239000002157 polynucleotide Substances 0.000 claims 4
- 102000040430 polynucleotide Human genes 0.000 claims 4
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 2
- 108700028146 Genetic Enhancer Elements Proteins 0.000 claims 2
- 241000209510 Liliopsida Species 0.000 claims 2
- 241000700605 Viruses Species 0.000 claims 2
- 230000009418 agronomic effect Effects 0.000 claims 2
- 230000011681 asexual reproduction Effects 0.000 claims 2
- 238000013465 asexual reproduction Methods 0.000 claims 2
- 235000013399 edible fruits Nutrition 0.000 claims 2
- 241001233957 eudicotyledons Species 0.000 claims 2
- 230000000977 initiatory effect Effects 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- 108020004999 messenger RNA Proteins 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 230000001850 reproductive effect Effects 0.000 claims 2
- 230000014639 sexual reproduction Effects 0.000 claims 2
- 230000002103 transcriptional effect Effects 0.000 claims 2
- 108010077805 Bacterial Proteins Proteins 0.000 claims 1
- 241001057636 Dracaena deremensis Species 0.000 claims 1
- 108700005088 Fungal Genes Proteins 0.000 claims 1
- 108700001094 Plant Genes Proteins 0.000 claims 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 claims 1
- 108700005077 Viral Genes Proteins 0.000 claims 1
- 230000009466 transformation Effects 0.000 claims 1
- 238000011144 upstream manufacturing Methods 0.000 claims 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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- General Health & Medical Sciences (AREA)
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- Plant Pathology (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physiology (AREA)
- Environmental Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Developmental Biology & Embryology (AREA)
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Claims (15)
1. Kimerno regulacijsko područje transkripcije, naznačeno time, da obuhvaća sljedeće:
jedna ili više kopija elemenata pojačivača virusa šećerne trske bacilnog oblika (SCBV-pojačivača) prikazanog na poziciji 337 do pozicije 618 od SEQ ID NO: 1, ili njegov homolog koji ima najmanje 90% identiteta s pozicijom 337 do pozicije 618 od SEQ ID NO: 1; i
s time operativno povezan jedan heterologni promotor koji obuhvaća mjesto vezanja RNK polimeraze i mjesto inicijacije mRNK,
pri čemu, kada se interesna nukleotidna sekvenca transkribira pod regulacijskim upravljanjem kimernog regulacijskog područja transkripcije, količina transkripcijskog produkta se pojačava u usporedbi prema količini transkripcijskog produkta koji se dobiva s kimernim regulacijskim područjem transkripcije koje obuhvaća promotor i ne obuhvaća sekvencu/sekvence SCBV-pojačivača.
2. Kimerno regulacijsko područje transkripcije prema zahtjevu 1, naznačeno time, da se promotor dobiva iz gornjeg područja biljnog virusnog gena, bakterijskog gena, gljivičnog gena, biljnog nuklearnog gena, biljnog izvan-nuklearnog gena, gena nekralježnjaka ili gena kralježnjaka.
3. Konstrukt, naznačen time, da obuhvaća regulacijsko područje transkripcije prema zahtjevu 1, koje je operativno povezano s polinukleotidnom molekulom koja se može transkribirati i koja je operativno povezana s polinukleotidnom molekulom 3'-terminalne transkripcije.
4. Konstrukt prema zahtjevu 3, naznačen time, da navedena polinukleotidna molekula koja se može transkribirati, dodjeljuje agronomsku značajku biljci u kojoj se ona izražava.
5. Transgenska biljka, naznačena time, da je ona stabilno transformirana s konstruktom prema zahtjevu 3, ili je to transformirana biljna stanica ili biljno tkivo transformirana/o s konstruktom prema zahtjevu 3.
6. Transgenska biljka prema zahtjevu 5, naznačena time, da polinukleotidna molekula koja se može transkribirati, dodjeljuje agronomsku značajku biljci u kojoj se ona izražava.
7. Sjeme transgenske biljke prema zahtjevu 5, naznačeno time, da sjeme obuhvaća konstrukt.
8. Transgenska biljka prema zahtjevu 5, naznačena time, da ta biljka je biljka kukuruza.
9. Transgenska biljna stanica, naznačena time, da ona obuhvaća kimerno regulacijsko područje transkripcije prema zahtjevu 1.
10. Postupak proizvodnje transgenske biljke, naznačen time, da obuhvaća transformaciju biljne stanice ili biljnog tkiva s konstruktom prema zahtjevu 3.
11. Postupak prema zahtjevu 10, naznačen time, da transgenska biljka je dikotiledon ili monokotiledon.
12. Biljna stanica ili biljno tkivo prema zahtjevu 5, naznačena/o time, da je biljna stanica ili biljno tkivo iz dikotiledona ili monokotiledona.
13. Biljna stanica, voćka, list, korijen, izbojak, cvijet, sjeme, sadnica i ostali reproduktivni materijal koristan u seksualnom ili aseksualnom razmnožavanju, potomstvo biljke s uključenim F1 hibridima, muške sterilne biljke i sve ostale biljke i biljni produkti izvedeni iz transgenske biljke prema zahtjevu 6, naznačeni time, da biljna stanica, voćka, list, korijen, izbojak, cvijet, sjeme, sadnica i ostali reproduktivni materijal koristan u seksualnom ili aseksualnom razmnožavanju, potomstvo biljke s uključenim F1 hibridima, muške sterilne biljke i sve ostale biljke i biljni produkti obuhvaćaju konstrukt prema zahtjevu 3.
14. Biljna stanica kukuruza, tkivo ili biljka kukuruza, naznačena time, da obuhvaća kimerno regulacijsko područje transkripcije koje obuhvaća jednu ili više kopija elemenata pojačivača virusa šećerne trske bacilnog oblika (SCBV-pojačivača) prikazanog na poziciji 337 do pozicije 618 od SEQ ID NO: 1, ili njegov homolog koji ima najmanje 90% identiteta s pozicijom 337 do pozicije 618 od SEQ ID NO: 1; i s time operativno povezan jedan heterologni promotor koji obuhvaća mjesto vezanja RNK polimeraze i mjesto inicijacije mRNK, gdje se kimerno regulacijsko područje transkripcije uvodi u genom biljne stanice kukuruza, tkivo ili biljku kukuruza na nasumičnim mjestima.
15. Biljna stanica kukuruza, tkivo ili biljka kukuruza prema zahtjevu 14, naznačena time, da SCBV-pojačivač dodjeljuje pojačanu transkripciju interesne nukleotidne sekvence koja je pod regulacijskim upravljanjem SCBV-pojačivača, u usporedbi s transkripcijom interesne nukleotidne sekvence u odsutnosti SCBV-pojačivača.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40257010P | 2010-08-30 | 2010-08-30 | |
| PCT/US2011/049532 WO2012030711A1 (en) | 2010-08-30 | 2011-08-29 | Sugarcane bacilliform viral (scbv) enhancer and its use in plant functional genomics |
| EP11758296.5A EP2611925B1 (en) | 2010-08-30 | 2011-08-29 | Sugarcane bacilliform viral (scbv) enhancer and its use in plant functional genomics |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20180349T1 true HRP20180349T1 (hr) | 2018-04-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20180349TT HRP20180349T1 (hr) | 2010-08-30 | 2018-02-27 | Pojačivač virusa šećerne trske u obliku bacila (scbv) i njegova uporaba u funkcionalnoj genomskoj tehnologiji biljaka |
Country Status (22)
| Country | Link |
|---|---|
| US (3) | US8785612B2 (hr) |
| EP (1) | EP2611925B1 (hr) |
| JP (1) | JP6039560B2 (hr) |
| KR (1) | KR20140016865A (hr) |
| CN (1) | CN103201389B (hr) |
| AR (1) | AR082784A1 (hr) |
| AU (2) | AU2011296209B2 (hr) |
| BR (1) | BR112013004948B8 (hr) |
| CA (1) | CA2809643C (hr) |
| CL (1) | CL2013000568A1 (hr) |
| DK (1) | DK2611925T3 (hr) |
| ES (1) | ES2657233T3 (hr) |
| HR (1) | HRP20180349T1 (hr) |
| HU (1) | HUE036163T2 (hr) |
| IL (1) | IL224935A (hr) |
| MX (1) | MX345696B (hr) |
| NZ (1) | NZ608144A (hr) |
| PL (1) | PL2611925T3 (hr) |
| PT (1) | PT2611925T (hr) |
| RS (1) | RS57012B1 (hr) |
| SI (1) | SI2611925T1 (hr) |
| WO (1) | WO2012030711A1 (hr) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8785612B2 (en) * | 2010-08-30 | 2014-07-22 | Agrigenetics, Inc. | Sugarcane bacilliform viral (SCBV) enhancer and its use in plant functional genomics |
| CA2865977A1 (en) * | 2012-02-29 | 2013-09-06 | Dow Agrosciences Llc | Sugarcane bacilliform viral (scbv) enhancer and its use in plant functional genomics |
| WO2017095698A1 (en) | 2015-11-30 | 2017-06-08 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
| US10961567B2 (en) | 2016-02-25 | 2021-03-30 | Roche Sequencing Solutions, Inc. | Elimination of primer-primer interactions during primer extension |
| EP3451837B1 (en) | 2016-05-04 | 2021-08-25 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| CN115266633B (zh) * | 2022-04-19 | 2024-08-13 | 安庆市长三角未来产业研究院 | 一种利用远红外吸收快速诊断番茄是否处于低温胁迫或弱光胁迫状态的方法 |
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