AR082784A1 - Potenciador viral baciliforme de caña de azucar (scbv) y su uso en la genomica funcional de plantas - Google Patents

Potenciador viral baciliforme de caña de azucar (scbv) y su uso en la genomica funcional de plantas

Info

Publication number
AR082784A1
AR082784A1 ARP110103136A ARP110103136A AR082784A1 AR 082784 A1 AR082784 A1 AR 082784A1 AR P110103136 A ARP110103136 A AR P110103136A AR P110103136 A ARP110103136 A AR P110103136A AR 082784 A1 AR082784 A1 AR 082784A1
Authority
AR
Argentina
Prior art keywords
transcription
enhancer
scbv
chimeric
promoter
Prior art date
Application number
ARP110103136A
Other languages
English (en)
Inventor
John P Davies
Vaka S Reddy
William M Ainley
Mark A Thompson
Original Assignee
Agrigenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agrigenetics Inc filed Critical Agrigenetics Inc
Publication of AR082784A1 publication Critical patent/AR082784A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Potenciador de la transcripción del badnavirus baciliforme de la caña de azúcar (SCBV). Este potenciador se puede usar para incrementar el índice de transcripción a partir de promotores de genes y en experimentos de activación etiquetada de genes. Se observó un incremento de diez veces en la transcripción cuando se ubicaba un ordenamiento 4x del potenciador SCBV 5’ con respecto de una forma truncada del promotor mínimo de alcohol deshidrogenasa de maíz. Se describen métodos para usar el potenciador de la transcripción SCBV, así como regiones reguladoras de la transcripción, construcciones, células, tejidos y organismos quiméricos que comprenden una o más copias del potenciador. Plantas. Semillas.Reivindicación 1: Una región reguladora de la transcripción quimérica que comprende una o más copias del elemento potenciador del virus baciliforme de caña de azúcar (SCBV) que se muestra entre la posición 337 y la posición 618 de la SEQ ID Nº 1, o un homólogo del mismo; y ligado operativamente al mismo, un promotor que comprende un sitio de unión a la ARN polimerasa y un sitio de inicio del ARNm, en donde cuando se transcribe una secuencia de nucleótidos de interés bajo el control regulador de la región reguladora de la transcripción quimérica, la cantidad de producto de transcripción aumenta en comparación con la cantidad de producto de transcripción obtenida con la región reguladora de la transcripción quimérica que comprende el promotor y pero que no comprende la o las secuencias del potenciador SCBV.
ARP110103136A 2010-08-30 2011-08-29 Potenciador viral baciliforme de caña de azucar (scbv) y su uso en la genomica funcional de plantas AR082784A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US40257010P 2010-08-30 2010-08-30

Publications (1)

Publication Number Publication Date
AR082784A1 true AR082784A1 (es) 2013-01-09

Family

ID=44654474

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110103136A AR082784A1 (es) 2010-08-30 2011-08-29 Potenciador viral baciliforme de caña de azucar (scbv) y su uso en la genomica funcional de plantas

Country Status (22)

Country Link
US (3) US8785612B2 (es)
EP (1) EP2611925B1 (es)
JP (1) JP6039560B2 (es)
KR (1) KR20140016865A (es)
CN (1) CN103201389B (es)
AR (1) AR082784A1 (es)
AU (2) AU2011296209B2 (es)
BR (1) BR112013004948B8 (es)
CA (1) CA2809643C (es)
CL (1) CL2013000568A1 (es)
DK (1) DK2611925T3 (es)
ES (1) ES2657233T3 (es)
HR (1) HRP20180349T1 (es)
HU (1) HUE036163T2 (es)
IL (1) IL224935A (es)
MX (1) MX345696B (es)
NZ (1) NZ608144A (es)
PL (1) PL2611925T3 (es)
PT (1) PT2611925T (es)
RS (1) RS57012B1 (es)
SI (1) SI2611925T1 (es)
WO (1) WO2012030711A1 (es)

Families Citing this family (6)

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Publication number Priority date Publication date Assignee Title
EP2611925B1 (en) * 2010-08-30 2017-11-29 Dow AgroSciences LLC Sugarcane bacilliform viral (scbv) enhancer and its use in plant functional genomics
WO2013130813A1 (en) * 2012-02-29 2013-09-06 Dow Agrosciences Llc Sugarcane bacilliform viral (scbv) enhancer and its use in plant functional genomics
US10995339B2 (en) * 2015-11-30 2021-05-04 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
EP3420100B1 (en) 2016-02-25 2021-04-07 H. Hoffnabb-La Roche Ag Elimination of primer-primer interactions during primer extension
EP3960863A1 (en) 2016-05-04 2022-03-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN115266633B (zh) * 2022-04-19 2024-08-13 安庆市长三角未来产业研究院 一种利用远红外吸收快速诊断番茄是否处于低温胁迫或弱光胁迫状态的方法

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