AR097495A1 - Constructos para expresar transgenes con el uso de elementos reguladores provenientes de genes ubiquitina 1c de brachypodium - Google Patents

Constructos para expresar transgenes con el uso de elementos reguladores provenientes de genes ubiquitina 1c de brachypodium

Info

Publication number
AR097495A1
AR097495A1 ARP140103239A ARP140103239A AR097495A1 AR 097495 A1 AR097495 A1 AR 097495A1 AR P140103239 A ARP140103239 A AR P140103239A AR P140103239 A ARP140103239 A AR P140103239A AR 097495 A1 AR097495 A1 AR 097495A1
Authority
AR
Argentina
Prior art keywords
sequence
seq
transgene
brachypodium
nucleic acid
Prior art date
Application number
ARP140103239A
Other languages
English (en)
Inventor
Kumar Sandeep
Gupta Manju
Alabed Diaa
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of AR097495A1 publication Critical patent/AR097495A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/14—Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce
    • A01H6/1464—Helianthus annuus [sunflower]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4636—Oryza sp. [rice]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4666—Sorghum, e.g. sudangrass
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4678—Triticum sp. [wheat]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542—Glycine max [soybean]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/60—Malvaceae, e.g. cotton or hibiscus
    • A01H6/604—Gossypium [cotton]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Heat Sensitive Colour Forming Recording (AREA)

Abstract

Reivindicación 1: Un vector de ácido nucleico que comprende un promotor operativamente unido a: i) una secuencia polienlazadora; ii) un transgen que no es de ubiquitina; o iii) una combinación de i) y ii), en donde dicho promotor comprende la SEC ID Nº 1 o una secuencia que posee un 90% de identidad de secuencia con la SEC ID Nº 1. Reivindicación 6: El vector de ácido nucleico de la reivindicación 5, en donde el transgen codifica un marcador seleccionable o un producto génico que confiere resistencia insecticida, tolerancia a herbicidas, eficacia en el uso de nitrógeno, eficacia en el uso del agua, o calidad nutricional. Reivindicación 14: Un vegetal que no es Brachypodium que comprende la SEC ID Nº 1, o una secuencia que posee un 90% de identidad de secuencia con la SEC ID Nº 1 operativamente unida a un transgen. Reivindicación 25: Un vector de ácido nucleico que comprende un terminador de la transcripción operativamente unido a: i) una secuencia polienlazadora; ii) un transgen que no es de ubiquitina; o iii) una combinación de i) y ii), en donde el terminador de la transcripción comprende la SEC ID Nº 4 o una secuencia que posee un 90% de identidad de secuencia con la SEC ID Nº 5.
ARP140103239A 2013-08-30 2014-08-29 Constructos para expresar transgenes con el uso de elementos reguladores provenientes de genes ubiquitina 1c de brachypodium AR097495A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361872134P 2013-08-30 2013-08-30

Publications (1)

Publication Number Publication Date
AR097495A1 true AR097495A1 (es) 2016-03-16

Family

ID=52585274

Family Applications (4)

Application Number Title Priority Date Filing Date
ARP140103238A AR097494A1 (es) 2013-08-30 2014-08-29 Constructos para expresar transgenes con el uso de elementos reguladores provenientes de genes de ubiquitina de setaria
ARP140103239A AR097495A1 (es) 2013-08-30 2014-08-29 Constructos para expresar transgenes con el uso de elementos reguladores provenientes de genes ubiquitina 1c de brachypodium
ARP140103236A AR097492A1 (es) 2013-08-30 2014-08-29 Constructos para expresar transgenes con el uso de elementos reguladores provenientes de genes de ubiquitina de panicum
ARP140103237A AR097493A1 (es) 2013-08-30 2014-08-29 Constructos para expresar transgenes con el uso de elementos reguladores provenientes de genes ubiquitina de brachypodium

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP140103238A AR097494A1 (es) 2013-08-30 2014-08-29 Constructos para expresar transgenes con el uso de elementos reguladores provenientes de genes de ubiquitina de setaria

Family Applications After (2)

Application Number Title Priority Date Filing Date
ARP140103236A AR097492A1 (es) 2013-08-30 2014-08-29 Constructos para expresar transgenes con el uso de elementos reguladores provenientes de genes de ubiquitina de panicum
ARP140103237A AR097493A1 (es) 2013-08-30 2014-08-29 Constructos para expresar transgenes con el uso de elementos reguladores provenientes de genes ubiquitina de brachypodium

Country Status (15)

Country Link
US (4) US9650640B2 (es)
EP (4) EP3039143A4 (es)
JP (4) JP2016528919A (es)
KR (4) KR20160046900A (es)
CN (4) CN105683381A (es)
AP (4) AP2016009110A0 (es)
AR (4) AR097494A1 (es)
AU (8) AU2014312166B2 (es)
BR (4) BR102014021330A2 (es)
CA (4) CA2922697A1 (es)
IL (4) IL244330A0 (es)
RU (4) RU2016111632A (es)
UY (4) UY35724A (es)
WO (4) WO2015031706A1 (es)
ZA (1) ZA201601478B (es)

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MX2017012936A (es) 2015-04-15 2018-02-01 Dow Agrosciences Llc Promotor de plantas para la expresion transgenica.
US10443065B2 (en) 2015-08-17 2019-10-15 Dow Agrosciences Llc Plant promotor and 3′ UTR for transgene expression
AU2017260655B2 (en) * 2016-05-02 2022-11-03 Corteva Agriscience Llc Plant promoter and 3'UTR for transgene expression
CA3020563A1 (en) * 2016-05-02 2017-11-09 Dow Agrosciences Llc Plant promoter and 3'utr for transgene expression
CA3024452A1 (en) * 2016-05-24 2017-11-30 Monsanto Technology Llc Plant regulatory elements and uses thereof
TW201805425A (zh) * 2016-06-16 2018-02-16 艾格里遺傳學股份有限公司 用於轉殖基因表現之植物啟動子與３’ｕｔｒ
CN107881172B (zh) * 2016-09-30 2021-06-08 江汉大学 一种逆境诱导型启动子、逆境诱导型启动子植物表达载体及诱导目标基因表达方法
EP3518657B1 (en) * 2016-10-03 2022-07-13 Corteva Agriscience LLC Plant promoter for transgene expression
WO2019005183A1 (en) * 2017-06-28 2019-01-03 Dow Agrosciences Llc PLANT PROMOTER FOR TRANSGENIC EXPRESSION
CN109486818B (zh) * 2018-11-14 2021-06-01 中国热带农业科学院三亚研究院 狗尾草u6启动子基因及应用
US11499159B2 (en) 2019-01-10 2022-11-15 Monsanto Technology Llc Plant regulatory elements and uses thereof
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US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants

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Also Published As

Publication number Publication date
IL244333A0 (en) 2016-04-21
US20150067924A1 (en) 2015-03-05
WO2015031733A1 (en) 2015-03-05
RU2016111662A (ru) 2017-10-05
CA2922697A1 (en) 2015-03-05
RU2016111674A3 (es) 2018-05-11
CN105683382A (zh) 2016-06-15
RU2016111608A (ru) 2017-10-05
AU2014312166B2 (en) 2017-06-08
JP2016531575A (ja) 2016-10-13
CA2922659A1 (en) 2015-03-05
AP2016009100A0 (en) 2016-03-31
AU2017203177B2 (en) 2019-06-20
US9758789B2 (en) 2017-09-12
BR102014021335A2 (pt) 2015-09-22
AU2017203180A1 (en) 2017-06-01
EP3039144A1 (en) 2016-07-06
RU2016111608A3 (es) 2018-04-25
AU2014312223A1 (en) 2016-03-17
AU2017203182B2 (en) 2019-06-20
AP2016009110A0 (en) 2016-03-31
BR102014021309A2 (pt) 2015-09-22
JP2016528918A (ja) 2016-09-23
AR097493A1 (es) 2016-03-16
AU2014312164B2 (en) 2017-06-08
AU2017203177A1 (en) 2017-06-01
EP3039143A4 (en) 2017-01-18
UY35724A (es) 2015-03-27
EP3039141A4 (en) 2017-03-08
EP3039143A1 (en) 2016-07-06
JP2016528919A (ja) 2016-09-23
CN106414746A (zh) 2017-02-15
EP3039142A4 (en) 2017-03-08
AU2014312222B2 (en) 2017-06-08
IL244331A0 (en) 2016-04-21
EP3039142A1 (en) 2016-07-06
US9708618B2 (en) 2017-07-18
AU2017203178A1 (en) 2017-06-01
CA2922656A1 (en) 2015-03-05
WO2015031705A1 (en) 2015-03-05
JP2016528916A (ja) 2016-09-23
RU2016111674A (ru) 2017-10-05
UY35725A (es) 2015-03-27
AR097492A1 (es) 2016-03-16
US20150067926A1 (en) 2015-03-05
WO2015031731A1 (en) 2015-03-05
UY35726A (es) 2015-03-27
EP3039144A4 (en) 2017-03-08
KR20160046901A (ko) 2016-04-29
KR20160048887A (ko) 2016-05-04
AP2016009109A0 (en) 2016-03-31
BR102014021330A2 (pt) 2015-09-22
AU2014312164A1 (en) 2016-03-17
US20150067925A1 (en) 2015-03-05
AP2016009098A0 (en) 2016-03-31
AU2017203178B2 (en) 2019-06-20
KR20160046900A (ko) 2016-04-29
US9752155B2 (en) 2017-09-05
UY35727A (es) 2015-03-27
BR102014021564A2 (pt) 2016-02-16
EP3039141A1 (en) 2016-07-06
US9650640B2 (en) 2017-05-16
CN105705644A (zh) 2016-06-22
AR097494A1 (es) 2016-03-16
IL244332A0 (en) 2016-04-21
ZA201601478B (en) 2018-05-30
WO2015031706A1 (en) 2015-03-05
AU2014312166A1 (en) 2016-03-17
RU2016111632A (ru) 2017-10-05
AU2017203182A1 (en) 2017-06-01
CA2922695A1 (en) 2015-03-05
IL244330A0 (en) 2016-04-21
US20150067927A1 (en) 2015-03-05
RU2016111662A3 (es) 2018-04-25
KR20160046899A (ko) 2016-04-29
AU2017203180B2 (en) 2019-06-20
CN105683381A (zh) 2016-06-15
AU2014312223B2 (en) 2017-06-08
AU2014312222A1 (en) 2016-03-17

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