CO2018000499A2 - Métodos y composiciones para la regulación selectiva de la expresión de proteínas - Google Patents

Métodos y composiciones para la regulación selectiva de la expresión de proteínas

Info

Publication number
CO2018000499A2
CO2018000499A2 CONC2018/0000499A CO2018000499A CO2018000499A2 CO 2018000499 A2 CO2018000499 A2 CO 2018000499A2 CO 2018000499 A CO2018000499 A CO 2018000499A CO 2018000499 A2 CO2018000499 A2 CO 2018000499A2
Authority
CO
Colombia
Prior art keywords
compositions
methods
protein expression
selective regulation
dna molecules
Prior art date
Application number
CONC2018/0000499A
Other languages
English (en)
Inventor
Jintai Huang
Youlin Qi
Yuanji Zhang
Heping Yang
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of CO2018000499A2 publication Critical patent/CO2018000499A2/es

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275Glyphosate
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12Processes for modifying agronomic input traits, e.g. crop yield
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A01H3/04Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823Reproductive tissue-specific promoters
    • C12N15/8231Male-specific, e.g. anther, tapetum, pollen
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289Male sterility
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/10923-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y205/00Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/010193-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

Abstract

La invención proporciona moléculas de ADN recombinante novedosas, composiciones y métodos para regular selectivamente la expresión de una molécula de polinucleótido que se puede transcribir o proteína recombinante en un tejido reproductor masculino de una planta transgénica. La invención también proporciona plantas, células de plantas, partes de plantas, semillas y productos básicos transgénicos que comprenden dichas composiciones y moléculas de ADN.
CONC2018/0000499A 2015-07-22 2018-01-19 Métodos y composiciones para la regulación selectiva de la expresión de proteínas CO2018000499A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562195546P 2015-07-22 2015-07-22
PCT/US2016/042217 WO2017015043A1 (en) 2015-07-22 2016-07-14 Methods and compositions for selective regulation of protein expression

Publications (1)

Publication Number Publication Date
CO2018000499A2 true CO2018000499A2 (es) 2018-05-31

Family

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Family Applications (1)

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CONC2018/0000499A CO2018000499A2 (es) 2015-07-22 2018-01-19 Métodos y composiciones para la regulación selectiva de la expresión de proteínas

Country Status (26)

Country Link
US (3) US10704057B2 (es)
EP (1) EP3324732B1 (es)
JP (1) JP6930960B2 (es)
KR (1) KR20180026777A (es)
CN (2) CN114891758B (es)
AR (2) AR105374A1 (es)
AU (3) AU2016296468B2 (es)
BR (1) BR112018001192B1 (es)
CA (1) CA2992113A1 (es)
CL (1) CL2018000165A1 (es)
CO (1) CO2018000499A2 (es)
EA (1) EA037898B1 (es)
EC (1) ECSP18003799A (es)
ES (1) ES2938362T3 (es)
HR (1) HRP20230022T1 (es)
HU (1) HUE061144T2 (es)
MA (1) MA42497A (es)
MX (2) MX2018000890A (es)
PE (1) PE20180465A1 (es)
PH (1) PH12018500119A1 (es)
PL (1) PL3324732T3 (es)
PT (1) PT3324732T (es)
RS (1) RS63980B1 (es)
UA (1) UA126893C2 (es)
WO (1) WO2017015043A1 (es)
ZA (1) ZA201800175B (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX354471B (es) 2011-07-01 2018-03-07 Monsanto Technology Llc Métodos y composiciones para la regulación selectiva de la expresión de proteínas.
HUE061144T2 (hu) 2015-07-22 2023-05-28 Monsanto Technology Llc Eljárások és készítmények fehérjeexpresszió szelektív szabályozására
UA124660C2 (uk) 2018-02-02 2021-10-20 Монсанто Текнолоджі Елелсі Об'єкт кукурудзи mon87429 і спосіб його використання
MY195997A (en) * 2018-04-18 2023-02-27 Kimagri Corp Sdn Bhd Recombinant Gene

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5583210A (en) 1993-03-18 1996-12-10 Pioneer Hi-Bred International, Inc. Methods and compositions for controlling plant development
US5750868A (en) * 1994-12-08 1998-05-12 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
US6271439B1 (en) 1998-03-04 2001-08-07 Pioneer Hi-Bred International, Inc. Methods and compositions for regulating cell death and enhancing disease resistance to plant pathogens
CN1300324A (zh) 1998-03-09 2001-06-20 孟山都公司 杀配子剂草甘膦
CN1249133A (zh) * 1998-12-25 2000-04-05 北京大学 获得雄性不育植物的分子方法
AR044724A1 (es) 2000-03-27 2005-10-05 Syngenta Participations Ag Promotores del virus de la rizadura amarilla del cestrum
US20020062499A1 (en) 2000-05-08 2002-05-23 Conner Timothy W. Methods for translational repression of gene expression in plants
US6646186B1 (en) 2000-07-26 2003-11-11 Stine Seed Farm Inc. Hybrid soybeans and methods of production
GB0108048D0 (en) 2001-03-30 2001-05-23 Isis Innovation Specification of meiocyte and tapetal cell layers in plants
US7977046B2 (en) * 2002-05-10 2011-07-12 Monsanto Technology Llc Method of selecting DNA constructs for herbicide tolerance in plants
US7230168B2 (en) 2001-12-20 2007-06-12 The Curators Of The University Of Missouri Reversible male sterility in transgenic plants by expression of cytokinin oxidase
US6967656B2 (en) 2002-10-28 2005-11-22 Syngenta Participations Ag Growing degree unit meter and method
AU2003903132A0 (en) 2003-06-20 2003-07-03 Molecular Plant Breeding Nominees Ltd. Plant promoter
HUE029170T2 (en) 2004-07-20 2017-03-28 Genentech Inc Preparations and methods for the use of angiopoietin-like protein 4
US7750207B2 (en) 2004-09-01 2010-07-06 Monsanto Technology Llc Zea mays ribulose bisphosphate carboxylase activase promoter
US20060200878A1 (en) 2004-12-21 2006-09-07 Linda Lutfiyya Recombinant DNA constructs and methods for controlling gene expression
WO2007047016A2 (en) 2005-10-13 2007-04-26 Monsanto Technology, Llc Methods for producing hybrid seed
ZA200803234B (en) * 2005-10-13 2009-01-28 Monsanto Technology Llc Methods for producing hybrid seed
US8158850B2 (en) 2007-12-19 2012-04-17 Monsanto Technology Llc Method to enhance yield and purity of hybrid crops
CA2710221A1 (en) * 2007-12-21 2009-07-09 E. I. Du Pont De Nemours And Company Drought tolerant plants and related constructs and methods involving genes encoding mir827
WO2009091518A2 (en) * 2008-01-15 2009-07-23 Monsanto Technology, Llc Isolated novel nucleic acid and protein molecules from corn and methods of using those molecules to generate transgenic plant with enhanced agronomic traits
EA201071408A1 (ru) * 2008-06-03 2011-06-30 Нордзаат Заатцухтгезелльшафт Мбх Способ получения однодольных растений с мужской стерильностью
US20120210458A9 (en) 2009-02-27 2012-08-16 Yongwei Cao Isolated Novel Nucleic Acid and Protein Molecules from Corn and Methods of Using Thereof
EP2503872B1 (en) 2009-11-23 2018-05-09 Monsanto Technology LLC Transgenic maize event mon 87427 and the relative development scale
WO2011079070A1 (en) 2009-12-23 2011-06-30 Gradalis, Inc. Furin-knockdown bi-functional rna
MX354471B (es) * 2011-07-01 2018-03-07 Monsanto Technology Llc Métodos y composiciones para la regulación selectiva de la expresión de proteínas.
HUE061144T2 (hu) 2015-07-22 2023-05-28 Monsanto Technology Llc Eljárások és készítmények fehérjeexpresszió szelektív szabályozására
UA124660C2 (uk) 2018-02-02 2021-10-20 Монсанто Текнолоджі Елелсі Об'єкт кукурудзи mon87429 і спосіб його використання

Also Published As

Publication number Publication date
AR105374A1 (es) 2017-09-27
ECSP18003799A (es) 2018-03-31
US20170029841A1 (en) 2017-02-02
US20200283790A1 (en) 2020-09-10
AU2016296468A1 (en) 2018-02-08
MA42497A (fr) 2018-05-30
ZA201800175B (en) 2018-12-19
CN107846856A (zh) 2018-03-27
AU2023229538A1 (en) 2023-10-05
US10704057B2 (en) 2020-07-07
HUE061144T2 (hu) 2023-05-28
EP3324732A1 (en) 2018-05-30
CA2992113A1 (en) 2017-01-26
MX2018000890A (es) 2018-05-15
RS63980B1 (sr) 2023-03-31
UA126893C2 (uk) 2023-02-22
BR112018001192B1 (pt) 2024-02-06
PH12018500119A1 (en) 2018-07-23
MX2021000869A (es) 2021-03-29
WO2017015043A1 (en) 2017-01-26
EA201890328A1 (ru) 2018-06-29
JP6930960B2 (ja) 2021-09-01
CN114891758A (zh) 2022-08-12
AU2016296468B2 (en) 2020-08-27
CN107846856B (zh) 2022-05-24
AU2020273330A1 (en) 2020-12-17
BR112018001192A2 (pt) 2018-09-25
HRP20230022T1 (hr) 2023-03-03
US20240102042A1 (en) 2024-03-28
CL2018000165A1 (es) 2018-04-27
US11866721B2 (en) 2024-01-09
AR122346A2 (es) 2022-09-07
EP3324732A4 (en) 2019-05-15
PT3324732T (pt) 2023-02-14
PE20180465A1 (es) 2018-03-06
JP2018521658A (ja) 2018-08-09
EA037898B1 (ru) 2021-06-03
AU2020273330B2 (en) 2023-07-20
KR20180026777A (ko) 2018-03-13
EP3324732B1 (en) 2022-11-23
PL3324732T3 (pl) 2023-03-13
ES2938362T3 (es) 2023-04-10
CN114891758B (zh) 2024-02-13

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