EA201890328A1 - Способы и композиции для селективной регуляции экспрессии белков - Google Patents

Способы и композиции для селективной регуляции экспрессии белков

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Publication number
EA201890328A1
EA201890328A1 EA201890328A EA201890328A EA201890328A1 EA 201890328 A1 EA201890328 A1 EA 201890328A1 EA 201890328 A EA201890328 A EA 201890328A EA 201890328 A EA201890328 A EA 201890328A EA 201890328 A1 EA201890328 A1 EA 201890328A1
Authority
EA
Eurasian Patent Office
Prior art keywords
compositions
methods
protein expression
selective regulation
dna molecules
Prior art date
Application number
EA201890328A
Other languages
English (en)
Other versions
EA037898B1 (ru
Inventor
Цзиньтай Хуан
Юлинь Ци
Хэпин Ян
Юаньцзин Чжан
Original Assignee
Монсанто Текнолоджи Ллс
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Монсанто Текнолоджи Ллс filed Critical Монсанто Текнолоджи Ллс
Publication of EA201890328A1 publication Critical patent/EA201890328A1/ru
Publication of EA037898B1 publication Critical patent/EA037898B1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A01H3/04—Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01019—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

Abstract

В изобретении предложены новые молекулы рекомбинантной ДНК, композиции и способы селективной регуляции экспрессии транскрибируемой полинуклеотидной молекулы или рекомбинантного белка в мужской репродуктивной ткани трансгенного растения. В изобретении также предложены трансгенные растения, растительные клетки, части растений, семена и товарные продукты, содержащие такие молекулы ДНК и композиции.
EA201890328A 2015-07-22 2016-07-14 Способы и композиции для селективной регуляции экспрессии белков EA037898B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562195546P 2015-07-22 2015-07-22
PCT/US2016/042217 WO2017015043A1 (en) 2015-07-22 2016-07-14 Methods and compositions for selective regulation of protein expression

Publications (2)

Publication Number Publication Date
EA201890328A1 true EA201890328A1 (ru) 2018-06-29
EA037898B1 EA037898B1 (ru) 2021-06-03

Family

ID=57835207

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201890328A EA037898B1 (ru) 2015-07-22 2016-07-14 Способы и композиции для селективной регуляции экспрессии белков

Country Status (26)

Country Link
US (3) US10704057B2 (ru)
EP (1) EP3324732B1 (ru)
JP (1) JP6930960B2 (ru)
KR (1) KR20180026777A (ru)
CN (2) CN107846856B (ru)
AR (2) AR105374A1 (ru)
AU (3) AU2016296468B2 (ru)
BR (1) BR112018001192B1 (ru)
CA (1) CA2992113A1 (ru)
CL (1) CL2018000165A1 (ru)
CO (1) CO2018000499A2 (ru)
EA (1) EA037898B1 (ru)
EC (1) ECSP18003799A (ru)
ES (1) ES2938362T3 (ru)
HR (1) HRP20230022T1 (ru)
HU (1) HUE061144T2 (ru)
MA (1) MA42497A (ru)
MX (2) MX2018000890A (ru)
PE (1) PE20180465A1 (ru)
PH (1) PH12018500119A1 (ru)
PL (1) PL3324732T3 (ru)
PT (1) PT3324732T (ru)
RS (1) RS63980B1 (ru)
UA (1) UA126893C2 (ru)
WO (1) WO2017015043A1 (ru)
ZA (1) ZA201800175B (ru)

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CN103635483B (zh) 2011-07-01 2016-11-09 孟山都技术公司 用于选择性调控蛋白质表达的方法和组合物
US10704057B2 (en) 2015-07-22 2020-07-07 Monsanto Technology Llc Methods and compositions for selective regulation of protein expression
EP3717650A4 (en) 2018-02-02 2021-09-01 Monsanto Technology LLC CORN VARIANT MON87429 AND METHOD OF USE THEREOF
MY195997A (en) * 2018-04-18 2023-02-27 Kimagri Corp Sdn Bhd Recombinant Gene

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EP3717650A4 (en) 2018-02-02 2021-09-01 Monsanto Technology LLC CORN VARIANT MON87429 AND METHOD OF USE THEREOF

Also Published As

Publication number Publication date
EA037898B1 (ru) 2021-06-03
PH12018500119A1 (en) 2018-07-23
HRP20230022T1 (hr) 2023-03-03
BR112018001192B1 (pt) 2024-02-06
AU2016296468A1 (en) 2018-02-08
AU2023229538A1 (en) 2023-10-05
CN107846856A (zh) 2018-03-27
CN107846856B (zh) 2022-05-24
EP3324732A4 (en) 2019-05-15
AU2020273330B2 (en) 2023-07-20
CN114891758A (zh) 2022-08-12
KR20180026777A (ko) 2018-03-13
CO2018000499A2 (es) 2018-05-31
PL3324732T3 (pl) 2023-03-13
CL2018000165A1 (es) 2018-04-27
JP6930960B2 (ja) 2021-09-01
CA2992113A1 (en) 2017-01-26
CN114891758B (zh) 2024-02-13
ZA201800175B (en) 2018-12-19
AR105374A1 (es) 2017-09-27
BR112018001192A2 (pt) 2018-09-25
US20170029841A1 (en) 2017-02-02
EP3324732A1 (en) 2018-05-30
US11866721B2 (en) 2024-01-09
AU2016296468B2 (en) 2020-08-27
HUE061144T2 (hu) 2023-05-28
US20200283790A1 (en) 2020-09-10
MX2018000890A (es) 2018-05-15
PE20180465A1 (es) 2018-03-06
RS63980B1 (sr) 2023-03-31
US20240102042A1 (en) 2024-03-28
PT3324732T (pt) 2023-02-14
MX2021000869A (es) 2021-03-29
MA42497A (fr) 2018-05-30
UA126893C2 (uk) 2023-02-22
JP2018521658A (ja) 2018-08-09
WO2017015043A1 (en) 2017-01-26
AU2020273330A1 (en) 2020-12-17
ECSP18003799A (es) 2018-03-31
EP3324732B1 (en) 2022-11-23
US10704057B2 (en) 2020-07-07
ES2938362T3 (es) 2023-04-10
AR122346A2 (es) 2022-09-07

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