CL2018000165A1 - Métodos y composiciones para la regulación selectiva de la expresión de proteínas - Google Patents

Métodos y composiciones para la regulación selectiva de la expresión de proteínas

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Publication number
CL2018000165A1
CL2018000165A1 CL2018000165A CL2018000165A CL2018000165A1 CL 2018000165 A1 CL2018000165 A1 CL 2018000165A1 CL 2018000165 A CL2018000165 A CL 2018000165A CL 2018000165 A CL2018000165 A CL 2018000165A CL 2018000165 A1 CL2018000165 A1 CL 2018000165A1
Authority
CL
Chile
Prior art keywords
compositions
methods
protein expression
selective regulation
dna molecules
Prior art date
Application number
CL2018000165A
Other languages
English (en)
Inventor
Jintai Huang
Youlin Qi
Yuanji Zhang
Heping Yang
Original Assignee
Montosanto Tech Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Montosanto Tech Llc filed Critical Montosanto Tech Llc
Publication of CL2018000165A1 publication Critical patent/CL2018000165A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A01H3/04—Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01019—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

Abstract

<p>LA INVENCIÓN PROPORCIONA MOLÉCULAS DE ADN RECOMBINANTE NOVEDOSAS, COMPOSICIONES Y MÉTODOS PARA REGULAR SELECTIVAMENTE LA EXPRESIÓN DE UNA MOLÉCULA DE POLITIUCLEÓTIDO QUE SE PUEDE TRANSCRIBIR O PROTEÍNA RECOMBINANTE EN UN TEJIDO REPRODUCTOR MASCULINO DE UNA PLANTA TRANSGÉNICA. LA INVENCIÓN TAMBIÉN PROPORCIONA PLANTAS, CÉLULAS DE PLANTAS, PARTES DE PLANTAS, SEMILLAS Y PRODUCTOS BÁSICOS TRANSGÉNICOS QUE COMPRENDEN DICHAS COMPOSICIONES Y MOLÉCULAS DE ADN.</p>
CL2018000165A 2015-07-22 2018-01-19 Métodos y composiciones para la regulación selectiva de la expresión de proteínas CL2018000165A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562195546P 2015-07-22 2015-07-22

Publications (1)

Publication Number Publication Date
CL2018000165A1 true CL2018000165A1 (es) 2018-04-27

Family

ID=57835207

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2018000165A CL2018000165A1 (es) 2015-07-22 2018-01-19 Métodos y composiciones para la regulación selectiva de la expresión de proteínas

Country Status (26)

Country Link
US (3) US10704057B2 (es)
EP (1) EP3324732B1 (es)
JP (1) JP6930960B2 (es)
KR (1) KR20180026777A (es)
CN (2) CN114891758B (es)
AR (2) AR105374A1 (es)
AU (3) AU2016296468B2 (es)
BR (1) BR112018001192B1 (es)
CA (1) CA2992113A1 (es)
CL (1) CL2018000165A1 (es)
CO (1) CO2018000499A2 (es)
EA (1) EA037898B1 (es)
EC (1) ECSP18003799A (es)
ES (1) ES2938362T3 (es)
HR (1) HRP20230022T1 (es)
HU (1) HUE061144T2 (es)
MA (1) MA42497A (es)
MX (2) MX2018000890A (es)
PE (1) PE20180465A1 (es)
PH (1) PH12018500119A1 (es)
PL (1) PL3324732T3 (es)
PT (1) PT3324732T (es)
RS (1) RS63980B1 (es)
UA (1) UA126893C2 (es)
WO (1) WO2017015043A1 (es)
ZA (1) ZA201800175B (es)

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EP2726493B1 (en) 2011-07-01 2020-10-14 Monsanto Technology LLC Methods and compositions for selective regulation of protein expression
BR112018001192B1 (pt) 2015-07-22 2024-02-06 Monsanto Technology Llc Molécula de dna recombinante, método para regular seletivamente a expressão de uma proteína em um tecido reprodutivo masculino de uma planta transgênica, e uso de uma planta ou semente que compreende a referida molécula de dna recombinante
BR112020014164A2 (pt) 2018-02-02 2020-12-08 Monsanto Technology Llc Evento de milho mon87429 e métodos de uso do mesmo
MY195997A (en) * 2018-04-18 2023-02-27 Kimagri Corp Sdn Bhd Recombinant Gene

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Also Published As

Publication number Publication date
ZA201800175B (en) 2018-12-19
CO2018000499A2 (es) 2018-05-31
US20240102042A1 (en) 2024-03-28
PL3324732T3 (pl) 2023-03-13
EP3324732A1 (en) 2018-05-30
AR105374A1 (es) 2017-09-27
AU2016296468B2 (en) 2020-08-27
CN107846856A (zh) 2018-03-27
US10704057B2 (en) 2020-07-07
WO2017015043A1 (en) 2017-01-26
CN114891758B (zh) 2024-02-13
US20170029841A1 (en) 2017-02-02
MA42497A (fr) 2018-05-30
EP3324732A4 (en) 2019-05-15
MX2021000869A (es) 2021-03-29
UA126893C2 (uk) 2023-02-22
CA2992113A1 (en) 2017-01-26
US20200283790A1 (en) 2020-09-10
AU2020273330B2 (en) 2023-07-20
CN114891758A (zh) 2022-08-12
HRP20230022T1 (hr) 2023-03-03
CN107846856B (zh) 2022-05-24
HUE061144T2 (hu) 2023-05-28
ECSP18003799A (es) 2018-03-31
RS63980B1 (sr) 2023-03-31
BR112018001192A2 (pt) 2018-09-25
AU2023229538A1 (en) 2023-10-05
US11866721B2 (en) 2024-01-09
KR20180026777A (ko) 2018-03-13
AU2016296468A1 (en) 2018-02-08
MX2018000890A (es) 2018-05-15
PT3324732T (pt) 2023-02-14
AR122346A2 (es) 2022-09-07
ES2938362T3 (es) 2023-04-10
EP3324732B1 (en) 2022-11-23
EA201890328A1 (ru) 2018-06-29
AU2020273330A1 (en) 2020-12-17
PH12018500119A1 (en) 2018-07-23
PE20180465A1 (es) 2018-03-06
BR112018001192B1 (pt) 2024-02-06
JP6930960B2 (ja) 2021-09-01
JP2018521658A (ja) 2018-08-09
EA037898B1 (ru) 2021-06-03

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