CO2019001678A2 - Métodos y composiciones para la expresión génica en plantas - Google Patents

Métodos y composiciones para la expresión génica en plantas

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Publication number
CO2019001678A2
CO2019001678A2 CONC2019/0001678A CO2019001678A CO2019001678A2 CO 2019001678 A2 CO2019001678 A2 CO 2019001678A2 CO 2019001678 A CO2019001678 A CO 2019001678A CO 2019001678 A2 CO2019001678 A2 CO 2019001678A2
Authority
CO
Colombia
Prior art keywords
compositions
methods
recombinant dna
dna molecules
plants
Prior art date
Application number
CONC2019/0001678A
Other languages
English (en)
Inventor
Yuanji Zhang
Clayton T Larue
Xuefeng Zhou
Joel E Ream
Aabid Shariff
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of CO2019001678A2 publication Critical patent/CO2019001678A2/es

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221Transit peptides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277Phosphinotricin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/001Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y103/00Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/03Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
    • C12Y103/03004Protoporphyrinogen oxidase (1.3.3.4)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/07Fusion polypeptide containing a localisation/targetting motif containing a mitochondrial localisation signal
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/08Fusion polypeptide containing a localisation/targetting motif containing a chloroplast localisation signal

Abstract

RESUMEN La invención proporciona moléculas de ADN recombinante útiles para proporcionar la expresión eficaz de proteínas en plantas transgénicas, así como composiciones y métodos para utilizar las moléculas de ADN recombinante. En modalidades particulares, la invención proporciona moléculas de ADN recombinante y construcciones que comprenden secuencias que codifican péptidos de tránsito y secuencias unidas operativamente que confieren tolerancia a herbicidas.
CONC2019/0001678A 2016-07-29 2019-02-25 Métodos y composiciones para la expresión génica en plantas CO2019001678A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662368840P 2016-07-29 2016-07-29
PCT/US2017/043990 WO2018022777A1 (en) 2016-07-29 2017-07-26 Methods and compositions for gene expression in plants

Publications (1)

Publication Number Publication Date
CO2019001678A2 true CO2019001678A2 (es) 2019-03-08

Family

ID=61016595

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2019/0001678A CO2019001678A2 (es) 2016-07-29 2019-02-25 Métodos y composiciones para la expresión génica en plantas

Country Status (20)

Country Link
US (3) US10745712B2 (es)
EP (2) EP3797583A3 (es)
JP (1) JP7118047B2 (es)
KR (1) KR102587506B1 (es)
CN (1) CN109475099A (es)
AR (4) AR109196A1 (es)
AU (2) AU2017301813B2 (es)
BR (1) BR112019000923A2 (es)
CA (1) CA2974824A1 (es)
CL (2) CL2019000220A1 (es)
CO (1) CO2019001678A2 (es)
EC (1) ECSP19013893A (es)
MX (3) MX2019001215A (es)
PE (2) PE20240136A1 (es)
PH (1) PH12019500209A1 (es)
RU (1) RU2763534C2 (es)
UA (1) UA127343C2 (es)
UY (1) UY37347A (es)
WO (1) WO2018022777A1 (es)
ZA (1) ZA201900225B (es)

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Also Published As

Publication number Publication date
MX2021010925A (es) 2021-10-01
PH12019500209A1 (en) 2019-12-02
AR109196A1 (es) 2018-11-07
CN109475099A (zh) 2019-03-15
KR20190033548A (ko) 2019-03-29
RU2019103990A3 (es) 2020-12-30
US20180044690A1 (en) 2018-02-15
UA127343C2 (uk) 2023-07-26
AR125392A2 (es) 2023-07-12
JP7118047B2 (ja) 2022-08-15
AR125394A2 (es) 2023-07-12
RU2763534C2 (ru) 2021-12-30
PE20240136A1 (es) 2024-01-30
EP3797583A3 (en) 2021-06-23
MX2019001215A (es) 2019-06-03
CA2974824A1 (en) 2018-01-29
EP3490366A1 (en) 2019-06-05
ECSP19013893A (es) 2019-03-29
WO2018022777A1 (en) 2018-02-01
MX2020013900A (es) 2021-03-09
AU2017301813B2 (en) 2023-06-15
US10745712B2 (en) 2020-08-18
EP3797583A2 (en) 2021-03-31
AR125393A2 (es) 2023-07-12
RU2019103990A (ru) 2020-08-28
PE20190838A1 (es) 2019-06-17
AU2023229539A1 (en) 2023-10-05
US20210002663A1 (en) 2021-01-07
US20230340519A1 (en) 2023-10-26
CL2020002234A1 (es) 2021-02-05
UY37347A (es) 2018-02-28
JP2019523004A (ja) 2019-08-22
ZA201900225B (en) 2019-10-30
BR112019000923A2 (pt) 2019-04-30
KR102587506B1 (ko) 2023-10-11
AU2017301813A1 (en) 2019-02-07
US11629358B2 (en) 2023-04-18
CL2019000220A1 (es) 2019-04-12
EP3490366A4 (en) 2020-04-22

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